##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142507_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	520336
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.133629039697425	33.0	33.0	33.0	27.0	33.0
2	31.207913732665048	33.0	33.0	33.0	27.0	33.0
3	31.3094404384859	33.0	33.0	33.0	27.0	33.0
4	31.252619461271177	33.0	33.0	33.0	27.0	33.0
5	31.304991390178653	33.0	33.0	33.0	27.0	33.0
6	34.637820562098334	37.0	37.0	37.0	27.0	37.0
7	34.62365279357953	37.0	37.0	37.0	27.0	37.0
8	34.625759124873156	37.0	37.0	37.0	27.0	37.0
9	34.53974162848621	37.0	37.0	37.0	27.0	37.0
10-11	34.62752913502045	37.0	37.0	37.0	27.0	37.0
12-13	34.560569516620035	37.0	37.0	37.0	27.0	37.0
14-15	34.550594231419694	37.0	37.0	37.0	27.0	37.0
16-17	34.539612865533044	37.0	37.0	37.0	27.0	37.0
18-19	34.58834099504935	37.0	37.0	37.0	27.0	37.0
20-21	34.57017196580671	37.0	37.0	37.0	27.0	37.0
22-23	34.650792949171304	37.0	37.0	37.0	27.0	37.0
24-25	34.74042157375234	37.0	37.0	37.0	27.0	37.0
26-27	34.809392392607855	37.0	37.0	37.0	27.0	37.0
28-29	34.86786903078011	37.0	37.0	37.0	27.0	37.0
30-31	34.80990263983273	37.0	37.0	37.0	27.0	37.0
32-33	34.836699747855235	37.0	37.0	37.0	27.0	37.0
34-35	34.8932439808124	37.0	37.0	37.0	27.0	37.0
36-37	34.86933827373082	37.0	37.0	37.0	27.0	37.0
38-39	34.84196269333661	37.0	37.0	37.0	27.0	37.0
40-41	34.81474950801021	37.0	37.0	37.0	27.0	37.0
42-43	34.82174306601888	37.0	37.0	37.0	27.0	37.0
44-45	34.802582369853326	37.0	37.0	37.0	27.0	37.0
46-47	34.78289316902924	37.0	37.0	37.0	27.0	37.0
48-49	34.774638887180586	37.0	37.0	37.0	27.0	37.0
50-51	34.69227191660774	37.0	37.0	37.0	27.0	37.0
52-53	34.71529934503859	37.0	37.0	37.0	27.0	37.0
54-55	34.686145106239046	37.0	37.0	37.0	27.0	37.0
56-57	34.6538256049937	37.0	37.0	37.0	27.0	37.0
58-59	34.60982615079487	37.0	37.0	37.0	27.0	37.0
60-61	34.58651429076597	37.0	37.0	37.0	27.0	37.0
62-63	34.56069155315027	37.0	37.0	37.0	27.0	37.0
64-65	34.52350980904646	37.0	37.0	37.0	27.0	37.0
66-67	34.48460898342609	37.0	37.0	37.0	27.0	37.0
68-69	34.481050705697854	37.0	37.0	37.0	27.0	37.0
70-71	34.43836482580487	37.0	37.0	37.0	27.0	37.0
72-73	34.38703260969835	37.0	37.0	37.0	27.0	37.0
74-75	34.31519249100582	37.0	37.0	37.0	27.0	37.0
76-77	34.28233968051413	37.0	37.0	37.0	27.0	37.0
78-79	34.228229451738876	37.0	37.0	37.0	27.0	37.0
80-81	34.17954744626549	37.0	37.0	37.0	27.0	37.0
82-83	34.13645221549153	37.0	37.0	37.0	27.0	37.0
84-85	34.090628939762	37.0	37.0	37.0	27.0	37.0
86-87	34.008627117862304	37.0	37.0	37.0	27.0	37.0
88-89	33.97163371360044	37.0	37.0	37.0	27.0	37.0
90-91	33.8826229590111	37.0	37.0	37.0	24.5	37.0
92-93	33.804965445404505	37.0	37.0	37.0	22.0	37.0
94-95	33.742275183727436	37.0	35.0	37.0	22.0	37.0
96-97	33.659358568309706	37.0	33.0	37.0	22.0	37.0
98-99	33.55980846991175	37.0	33.0	37.0	22.0	37.0
100-101	33.461096291626944	37.0	33.0	37.0	22.0	37.0
102-103	33.35462470403739	37.0	33.0	37.0	22.0	37.0
104-105	33.220492143538024	37.0	33.0	37.0	18.0	37.0
106-107	33.13027447649211	37.0	33.0	37.0	14.0	37.0
108-109	33.01976030872359	37.0	33.0	37.0	14.0	37.0
110-111	32.95060115002614	37.0	33.0	37.0	14.0	37.0
112-113	32.802713054641615	37.0	33.0	37.0	14.0	37.0
114-115	32.69709476184619	37.0	33.0	37.0	14.0	37.0
116-117	32.498490398511734	37.0	33.0	37.0	14.0	37.0
118-119	32.42390974293534	37.0	33.0	37.0	14.0	37.0
120-121	32.317244049998465	37.0	33.0	37.0	14.0	37.0
122-123	32.23672876756557	37.0	33.0	37.0	14.0	37.0
124-125	30.664954952184743	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1025.0
1	62.0
2	19.0
3	24.0
4	20.0
5	19.0
6	22.0
7	33.0
8	33.0
9	23.0
10	13.0
11	11.0
12	15.0
13	23.0
14	45.0
15	170.0
16	280.0
17	619.0
18	1178.0
19	2424.0
20	3586.0
21	4427.0
22	4494.0
23	4916.0
24	5197.0
25	6207.0
26	7134.0
27	8434.0
28	10048.0
29	12165.0
30	14282.0
31	17296.0
32	21290.0
33	28617.0
34	42765.0
35	80463.0
36	242957.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.250737076084775	20.41797230754982	12.603615145948782	23.72767547041662
2	17.21250766684797	23.21270825859362	36.10477832683338	23.47000574772502
3	19.485185635186504	28.06812505665195	28.350468740658453	24.09622056750309
4	14.38100582575946	19.71598217367744	33.95486338102318	31.948148619539918
5	14.906182974061444	38.322056259354696	30.842733038097737	15.929027728486119
6	28.83704971625956	36.48898192696768	18.468958179126574	16.205010177646187
7	25.783843173538422	34.28400701632645	21.282985408354055	18.649164401781068
8	25.38432479106917	34.39995990995158	20.306187744780583	19.90952755419866
9	26.623217655642495	18.695604401959255	19.751951253833592	34.929226688564654
10-11	24.805799604467282	27.833494604794968	25.49884925423193	21.86185653650582
12-13	25.60623122399318	26.93091586420729	25.171900488610028	22.2909524231895
14-15	24.28787501712187	26.848534657553007	23.512905547099123	25.350684778226
16-17	23.554839026939568	28.97688648817519	24.14215036365863	23.326124121226613
18-19	23.77379809268193	27.783465796688994	25.64515631447225	22.79757979615683
20-21	25.965987522460342	26.347427760597814	25.004048645438914	22.682536071502934
22-23	25.657392484519466	26.190439469767657	24.90052360058298	23.251644445129898
24-25	24.369407746367568	26.969552696810705	24.960100077486803	23.700939479334924
26-27	23.999876635067253	27.013643390530973	25.01889025532686	23.967589719074915
28-29	23.72646637061708	26.912179665093905	25.49630708977087	23.865046874518146
30-31	24.388666636469036	26.7128561914941	25.30750986161454	23.590967310422325
32-33	23.795610027339755	27.110784314670276	25.040402512135206	24.053203145854756
34-35	24.039452916474755	26.91025386541067	25.348286846907957	23.702006371206615
36-37	24.00879592735458	27.048151149647364	24.966056470084887	23.97699645291317
38-39	24.03197428490491	26.89322184472661	24.99792837775598	24.076875492612498
40-41	23.76641210339926	27.15276205160327	25.20475077693994	23.876075068057528
42-43	24.03018310867519	26.893357962379998	25.173564437815028	23.902894491129786
44-45	23.90276944066799	27.073515483257427	24.976417889451692	24.0472971866229
46-47	23.969237054280295	26.932319206992773	25.103095564770996	23.99534817395594
48-49	23.919980268685016	27.06600741465821	25.14586528752996	23.868147029126813
50-51	23.617706993482	27.276073205863792	25.041357748134445	24.064862052519764
52-53	23.680719147890684	26.94029742893067	25.214592998328346	24.1643904248503
54-55	23.58675271568943	26.965153229054454	25.208361837553884	24.23973221770223
56-57	23.817171926410158	26.67809984439959	25.02247293853543	24.482255290654823
58-59	23.84322060024972	26.688202333790638	25.234400289796987	24.234176776162656
60-61	23.790063477291625	26.741964665046257	25.3805121150202	24.087459742641922
62-63	23.514960702745512	26.80537117070943	25.223172566874695	24.456495559670362
64-65	23.48840704247604	26.586252605862565	25.336016585038784	24.58932376662261
66-67	23.851112857979594	26.449547612441815	25.108087487283825	24.59125204229477
68-69	23.84499625227128	26.30375678493047	25.015752021750316	24.83549494104794
70-71	23.626911957241138	26.3224621799275	25.34587917037168	24.704746692459683
72-73	23.79172129323607	26.38277868701649	25.340017281422277	24.485482738325164
74-75	23.832274523631977	26.22630202763503	25.40497785074155	24.53644559799144
76-77	23.675147623379225	26.119798492160157	25.480354141934292	24.724699742526326
78-79	23.73785205788813	26.238591508315086	25.357802478580023	24.66575395521676
80-81	23.458526795306234	26.35161631275852	25.55721383927152	24.63264305266373
82-83	23.67456101306604	26.237188787985644	25.62252037172988	24.465729827218443
84-85	23.586289953513017	26.4285955150902	25.272924344244522	24.71219018715225
86-87	23.390641046143532	26.57226918119269	25.456878563267445	24.58021120939633
88-89	23.65423293878306	26.107649805807288	25.47536757906418	24.76274967634548
90-91	23.895822938680052	26.118914191374635	25.43811657551479	24.547146294430522
92-93	23.720314208242428	26.382607774294065	25.362249738721687	24.53482827874182
94-95	23.116382776870807	26.59818638003188	25.6019956561731	24.683435186924214
96-97	23.26965100724143	26.405645213327926	25.516333423542203	24.80837035588844
98-99	23.12118909412763	26.661870378877843	25.39875161682091	24.818188910173618
100-101	23.382446408967468	26.804279578590553	25.200978409506753	24.612295602935227
102-103	23.442641738020008	26.697336323897776	25.421691736238376	24.43833020184384
104-105	23.23300421066419	26.966453372576364	25.40758304793531	24.392959368824137
106-107	23.240624819430266	26.925210423929585	25.469385003563243	24.36477975307691
108-109	23.217962422596518	27.025106173980873	25.24985795317751	24.50707345024509
110-111	23.12396353571793	27.316431461903377	25.12110140159825	24.438503600780443
112-113	23.181696931994257	27.3087082674083	25.077930369684147	24.431664430913294
114-115	23.004697096043	27.42062215819912	25.166536031290864	24.408144714467017
116-117	22.99763300161973	27.320727317549494	25.269007728856387	24.412631951974394
118-119	22.96412343180236	27.19595814480618	25.341033939220036	24.498884484171423
120-121	22.986047586492447	27.219627743059903	25.3968590218867	24.397465648560946
122-123	22.959592380629605	27.43381577021729	25.299725788934506	24.3068660602186
124-125	22.942596331503033	27.38394052917939	25.081188915751273	24.592274223566303
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2230.0
1	1369.5
2	412.5
3	296.5
4	240.5
5	199.0
6	176.5
7	155.0
8	153.5
9	154.5
10	149.0
11	127.5
12	128.0
13	139.5
14	135.5
15	147.0
16	163.0
17	176.0
18	201.5
19	243.5
20	296.5
21	357.5
22	425.0
23	545.5
24	723.0
25	930.0
26	1198.5
27	1596.5
28	2100.5
29	2950.0
30	4006.0
31	5020.0
32	6258.5
33	7461.5
34	8554.0
35	9476.5
36	10314.5
37	11477.5
38	11993.0
39	12052.0
40	12204.0
41	12107.5
42	12511.5
43	13678.0
44	14909.5
45	16084.0
46	17536.5
47	18982.5
48	20327.0
49	21377.5
50	21826.0
51	21564.0
52	21068.0
53	20978.0
54	20602.0
55	19840.5
56	18716.0
57	17301.0
58	16075.5
59	14435.5
60	12718.0
61	10704.0
62	8533.0
63	6569.5
64	5037.5
65	4154.0
66	3266.0
67	2448.5
68	1947.0
69	1630.0
70	1364.5
71	1178.5
72	1005.0
73	936.0
74	773.5
75	670.5
76	558.0
77	379.0
78	266.0
79	149.0
80	87.5
81	71.5
82	60.0
83	34.0
84	18.0
85	13.0
86	9.0
87	2.5
88	1.0
89	1.5
90	0.5
91	0.5
92	1.0
93	0.5
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3982042372620768
2	0.35938316779926816
3	0.3495818086774699
4	0.3415101011654008
5	0.3613050029211894
6	0.29826881092217333
7	0.29711570984902064
8	0.28904400233695154
9	0.3011515636050552
10-11	0.2963469758002521
12-13	0.3309400079948341
14-15	0.3830217397988992
16-17	0.34333584453122595
18-19	0.3270963377509917
20-21	0.3159496940438486
22-23	0.31133728975123764
24-25	0.2955782417514837
26-27	0.2978844438977891
28-29	0.28904400233695154
30-31	0.2943290489222349
32-33	0.28721825897112635
34-35	0.2765520740444636
36-37	0.2804918360444021
38-39	0.2727084038006211
40-41	0.2831824052150918
42-43	0.27607161526398327
44-45	0.26992174287383536
46-47	0.26905691706897084
48-49	0.26213831063005444
50-51	0.26684680667876143
52-53	0.26684680667876143
54-55	0.27040220165431567
56-57	0.2649249715568402
58-59	0.259832108483749
60-61	0.25589234648381043
62-63	0.25810245687401984
64-65	0.2525291350204483
66-67	0.25214476799606406
68-69	0.26040865902032534
70-71	0.2560845299960026
72-73	0.24628317087420437
74-75	0.26079302604470955
76-77	0.2782817256541927
78-79	0.2631953199471111
80-81	0.2676155407275299
82-83	0.26079302604470955
84-85	0.2639640539958796
86-87	0.25464315365456164
88-89	0.2429199594108422
90-91	0.23811537160603916
92-93	0.24090203253282497
94-95	0.23225377448417944
96-97	0.2249508010208788
98-99	0.22764137019156855
100-101	0.21639863472832938
102-103	0.22053058024046002
104-105	0.22639217736231976
106-107	0.22024230497217184
108-109	0.21928138741121123
110-111	0.21966575443559547
112-113	0.21572599243565696
114-115	0.20736600965529967
116-117	0.2139002490698318
118-119	0.20554026628947447
120-121	0.2058285415577627
122-123	0.19756465053350145
124-125	0.19650764121644476
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	520336.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.26348747765724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.3112874649941	67.45974565709702
2	8.791511182965799	13.585256283895228
3	1.959503634680935	4.541942536217827
4	0.7143283505841407	2.2076599828117325
5	0.34296110991609347	1.3249185710662759
6	0.200381594171308	0.9289308475204708
7	0.1329580581395789	0.7190962282084684
8	0.09489720850011797	0.5865671424490794
9	0.05934816310128728	0.41269014509384416
>10	0.35793666244817135	5.178115514507859
>50	0.026746370952009664	1.4431931924746633
>100	0.0075587567216607865	0.9359977990114096
>500	0.0	0.0
>1k	5.814428247431346E-4	0.6758860996461057
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1861	0.35765351618953906	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	1148	0.22062667199655608	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	2.88275268288183E-4	0.0
18-19	0.0	0.0	0.0	3.84367024384244E-4	0.0
20-21	0.0	0.0	0.0	8.64825804864549E-4	0.0
22-23	0.0	0.0	0.0	0.004420220780418807	0.0
24-25	0.0	0.0	0.0	0.008263891024261246	0.0
26-27	0.0	0.0	0.0	0.012011469512007626	0.0
28-29	0.0	0.0	0.0	0.02498385658497586	0.0
30-31	0.0	0.0	0.0	0.0458357676578211	0.0
32-33	0.0	0.0	0.0	0.07869914824267396	0.0
34-35	0.0	0.0	0.0	0.11732803419329049	0.0
36-37	0.0	0.0	0.0	0.17949940038744194	0.0
38-39	0.0	0.0	0.0	0.2640601457519757	0.0
40-41	0.0	0.0	0.0	0.34833261584822117	0.0
42-43	0.0	0.0	0.0	0.4343347375541957	0.0
44-45	0.0	0.0	0.0	0.521201685065035	0.0
46-47	0.0	0.0	0.0	0.6214253866732266	0.0
48-49	0.0	0.0	0.0	0.7302012545739676	0.0
50-51	0.0	0.0	0.0	0.830040589157775	0.0
52-53	0.0	0.0	0.0	0.9263245287660281	0.0
54-55	0.0	0.0	0.0	1.0257794963254514	0.0
56-57	0.0	0.0	0.0	1.1358045570554411	0.0
58-59	0.0	0.0	0.0	1.261973032809569	0.0
60-61	0.0	0.0	0.0	1.3817994526613573	0.0
62-63	0.0	0.0	0.0	1.4928815227084038	0.0
64-65	9.6091756096061E-5	0.0	0.0	1.5966606192921495	9.6091756096061E-5
66-67	1.92183512192122E-4	0.0	0.0	1.7247509301681991	1.92183512192122E-4
68-69	1.92183512192122E-4	0.0	0.0	1.860240306263645	1.92183512192122E-4
70-71	1.92183512192122E-4	0.0	0.0	2.0056271332369855	1.92183512192122E-4
72-73	1.92183512192122E-4	0.0	0.0	2.1476507487469636	1.92183512192122E-4
74-75	2.88275268288183E-4	0.0	0.0	2.2741074997693795	1.92183512192122E-4
76-77	3.84367024384244E-4	0.0	0.0	2.4154584729866855	1.92183512192122E-4
78-79	3.84367024384244E-4	0.0	0.0	2.5859252483010975	1.92183512192122E-4
80-81	3.84367024384244E-4	0.0	0.0	2.757929491713047	1.92183512192122E-4
82-83	3.84367024384244E-4	0.0	0.0	2.9305102856615726	1.92183512192122E-4
84-85	3.84367024384244E-4	0.0	0.0	3.096845115463854	1.92183512192122E-4
86-87	3.84367024384244E-4	0.0	0.0	3.2671197072660743	1.92183512192122E-4
88-89	3.84367024384244E-4	0.0	0.0	3.4388356754097353	1.92183512192122E-4
90-91	3.84367024384244E-4	0.0	0.0	3.6394752621383106	1.92183512192122E-4
92-93	3.84367024384244E-4	0.0	0.0	3.8309861320377605	1.92183512192122E-4
94-95	3.84367024384244E-4	0.0	0.0	4.02835859905907	1.92183512192122E-4
96-97	3.84367024384244E-4	0.0	0.0	4.232361397251006	1.92183512192122E-4
98-99	3.84367024384244E-4	0.0	0.0	4.447318655637895	1.92183512192122E-4
100-101	3.84367024384244E-4	0.0	0.0	4.688220688170721	1.92183512192122E-4
102-103	3.84367024384244E-4	0.0	0.0	4.916726884167154	1.92183512192122E-4
104-105	3.84367024384244E-4	0.0	0.0	5.15051812674887	1.92183512192122E-4
106-107	3.84367024384244E-4	0.0	0.0	5.398915316257187	1.92183512192122E-4
108-109	3.84367024384244E-4	0.0	0.0	5.649618707911811	1.92183512192122E-4
110-111	3.84367024384244E-4	0.0	0.0	5.927708250053811	1.92183512192122E-4
112-113	3.84367024384244E-4	0.0	0.0	6.209161003659174	1.92183512192122E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1860	0.0	38.779232	1
ATTAGAC	100	1.0517588E-5	35.7459	3
GTATCAA	2610	0.0	32.203667	1
ATGGGTG	660	0.0	29.788252	5
CATGGGT	1450	0.0	27.11758	4
TACACGG	90	0.009464752	26.478445	2
ATCAACG	3180	0.0	25.666605	3
AACGCAG	3240	0.0	25.549181	6
CAACGCA	3295	0.0	25.132421	5
GTATTAG	190	1.1473476E-6	25.099388	1
TCAACGC	3255	0.0	25.075207	4
TATCAAC	3365	0.0	24.786655	2
GTACATG	5220	0.0	23.067877	1
TACATGG	5310	0.0	22.551556	2
ACATGGG	5160	0.0	22.398918	3
ACGCAGA	3815	0.0	21.39031	7
CGCAGAG	3880	0.0	20.880466	8
CATGGGG	2375	0.0	20.318722	4
GCAGAGT	4090	0.0	19.67792	9
ATGGGGG	1215	0.0	19.123322	5
>>END_MODULE