FastQCFastQC Report
Sun 1 Jan 2017
ERR1142507_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142507_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520336
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32600.6265182497463178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32120.617293441161096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16930.3253666861412626No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7990.15355462624150548No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6320.1214599797054211No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG1550.057.5595781
GGTATCA26300.050.6582641
GTATCAA39000.035.3819241
TAGGACG700.00279259533.987564
ATCAACG48200.028.6285253
TATCAAC49050.028.3749372
TCAACGC49450.027.9048544
CAACGCA49850.027.8002595
AACGCAG51850.026.7279226
CATGGGT14000.026.340364
GAGTACT25250.025.08444812-13
ACGCAGA55500.024.9677417
CGCAGAG57050.024.289398
GTACTAG1802.1802467E-523.1304231
ATGGGTG6750.022.9101335
GCAGAGT60450.022.8248529
CAGAGTA60150.022.6915110-11
ACATGGG48850.022.6467783
GTACATG50450.022.5180231
GTATTAT1602.6150665E-422.3043371
Produced by FastQC (version 0.11.5)