##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142507_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	520336
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.220945696626796	27.0	14.0	33.0	14.0	33.0
2	29.014955720918792	33.0	27.0	33.0	27.0	33.0
3	31.380654038928693	33.0	33.0	33.0	27.0	33.0
4	31.099214738169184	33.0	33.0	33.0	27.0	33.0
5	32.12641254881461	33.0	33.0	33.0	33.0	33.0
6	35.176061237354325	37.0	37.0	37.0	33.0	37.0
7	35.71566641554688	37.0	37.0	37.0	33.0	37.0
8	35.60637165216322	37.0	37.0	37.0	33.0	37.0
9	35.97365356231358	37.0	37.0	37.0	33.0	37.0
10-11	36.05636069770303	37.0	37.0	37.0	35.0	37.0
12-13	36.10558369976323	37.0	37.0	37.0	37.0	37.0
14-15	36.085619676516714	37.0	37.0	37.0	37.0	37.0
16-17	36.07110309492328	37.0	37.0	37.0	37.0	37.0
18-19	36.06598236524092	37.0	37.0	37.0	37.0	37.0
20-21	36.09251714276929	37.0	37.0	37.0	37.0	37.0
22-23	36.09890820546724	37.0	37.0	37.0	37.0	37.0
24-25	36.07887019156853	37.0	37.0	37.0	37.0	37.0
26-27	36.03977237784816	37.0	37.0	37.0	37.0	37.0
28-29	36.0110861059008	37.0	37.0	37.0	37.0	37.0
30-31	35.94171074075213	37.0	37.0	37.0	35.0	37.0
32-33	35.896022954398696	37.0	37.0	37.0	33.0	37.0
34-35	35.823945489068606	37.0	37.0	37.0	33.0	37.0
36-37	35.7677788967129	37.0	37.0	37.0	33.0	37.0
38-39	35.71500338242981	37.0	37.0	37.0	33.0	37.0
40-41	35.576039328433936	37.0	37.0	37.0	33.0	37.0
42-43	35.52191276406015	37.0	37.0	37.0	33.0	37.0
44-45	35.42410384828265	37.0	37.0	37.0	33.0	37.0
46-47	35.25475077642139	37.0	37.0	37.0	33.0	37.0
48-49	35.10888541250269	37.0	37.0	37.0	33.0	37.0
50-51	34.99649649457274	37.0	37.0	37.0	30.0	37.0
52-53	34.917690684480796	37.0	37.0	37.0	27.0	37.0
54-55	34.866662118323546	37.0	37.0	37.0	27.0	37.0
56-57	34.81667710863749	37.0	37.0	37.0	27.0	37.0
58-59	34.759655299652536	37.0	37.0	37.0	27.0	37.0
60-61	34.72989087820178	37.0	37.0	37.0	27.0	37.0
62-63	34.716914647458566	37.0	37.0	37.0	27.0	37.0
64-65	34.681927639063986	37.0	37.0	37.0	27.0	37.0
66-67	34.64251176163094	37.0	37.0	37.0	27.0	37.0
68-69	34.60716440915101	37.0	37.0	37.0	27.0	37.0
70-71	34.57021712893207	37.0	37.0	37.0	27.0	37.0
72-73	34.54298376433689	37.0	37.0	37.0	27.0	37.0
74-75	34.447511800067645	37.0	37.0	37.0	27.0	37.0
76-77	34.39236089757387	37.0	37.0	37.0	27.0	37.0
78-79	34.37163390578395	37.0	37.0	37.0	27.0	37.0
80-81	34.34169748008979	37.0	37.0	37.0	27.0	37.0
82-83	34.28820031671843	37.0	37.0	37.0	27.0	37.0
84-85	34.21030930014452	37.0	37.0	37.0	27.0	37.0
86-87	34.03556644168384	37.0	37.0	37.0	27.0	37.0
88-89	33.962561690907414	37.0	37.0	37.0	27.0	37.0
90-91	33.90815934319363	37.0	37.0	37.0	24.5	37.0
92-93	33.852767250392056	37.0	37.0	37.0	22.0	37.0
94-95	33.85157090802866	37.0	37.0	37.0	22.0	37.0
96-97	33.80199428830602	37.0	37.0	37.0	22.0	37.0
98-99	33.7348203852895	37.0	33.0	37.0	22.0	37.0
100-101	33.68333826604348	37.0	33.0	37.0	22.0	37.0
102-103	33.56733245441407	37.0	33.0	37.0	22.0	37.0
104-105	33.46773431013807	37.0	33.0	37.0	22.0	37.0
106-107	33.39347556194459	37.0	33.0	37.0	14.0	37.0
108-109	33.2880532963316	37.0	33.0	37.0	14.0	37.0
110-111	33.2065905491836	37.0	33.0	37.0	14.0	37.0
112-113	33.155106508102456	37.0	33.0	37.0	14.0	37.0
114-115	33.031684334737555	37.0	33.0	37.0	14.0	37.0
116-117	32.90174714030934	37.0	33.0	37.0	14.0	37.0
118-119	32.74201477506842	37.0	33.0	37.0	14.0	37.0
120-121	32.651932597398606	37.0	33.0	37.0	14.0	37.0
122-123	32.521981950124534	37.0	33.0	37.0	14.0	37.0
124-125	31.231089142400297	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	137.0
16	53.0
17	40.0
18	120.0
19	434.0
20	1439.0
21	3220.0
22	5072.0
23	7134.0
24	6389.0
25	4856.0
26	4791.0
27	5208.0
28	6526.0
29	8306.0
30	10976.0
31	14644.0
32	20435.0
33	29949.0
34	48605.0
35	99593.0
36	242403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.89801826601641	14.807161078121725	12.72123659893003	21.573584056931843
2	18.538213769564283	20.8284646843578	38.01389871160173	22.619422834476186
3	20.299923894744126	27.00641129125251	28.973424659640383	23.720240154362983
4	14.53234064143169	18.77709787521909	34.561321915070266	32.129239568278955
5	15.861017900316341	35.56116742196239	32.25477873487006	16.323035942851213
6	28.994372823504992	34.74397115687676	19.04072784299255	17.220928176625694
7	25.936275960684675	32.553151414744406	22.728740395075206	18.78183222949572
8	26.253997417053597	31.393561083607512	21.383298484056457	20.969143015282434
9	27.26238430552566	15.95507518218997	20.991244119184525	35.79129639309984
10-11	25.521009501552843	25.250222932874145	26.583592140463086	22.64517542510993
12-13	26.87157913348298	24.513199163617355	26.716775314412228	21.89844638848744
14-15	24.910634666830664	24.49965022600781	25.94458196242428	24.645133144737247
16-17	23.015513053104147	26.725807939485257	26.778562313581993	23.480116693828602
18-19	22.970926478275576	24.971652931951663	29.615094861781614	22.442325727991143
20-21	24.914237942863732	23.641596279320055	29.401155025128045	22.043010752688172
22-23	24.624665600688786	23.55362688724209	29.430694781833278	22.39101273023585
24-25	23.394979397927493	24.43430783186249	29.48287260539344	22.68784016481658
26-27	23.14936886934596	24.471207066203377	29.510450939393007	22.868973125057654
28-29	22.86666692291135	24.426140032594322	29.85426724270471	22.852925801789613
30-31	23.316856800221395	24.23078549245103	29.72617693182866	22.726180775498907
32-33	22.908562928569232	24.357242243473447	29.544179145782724	23.190015682174593
34-35	23.264871160173424	24.425371298545556	29.471053319393626	22.838704221887397
36-37	23.213173795393747	24.44285999815504	29.194309061836965	23.14965714461425
38-39	23.166569293687157	24.340041819132253	29.29693505734756	23.19645382983303
40-41	22.95234928737258	24.582282713980412	29.390980304730864	23.074387693916147
42-43	23.281398942221948	24.39116263337536	29.155584084130254	23.17185434027244
44-45	23.26746563758802	24.60535115771348	28.813497432428274	23.313685772270226
46-47	23.35670746649318	24.58752518957649	28.570096933622523	23.48567041030781
48-49	23.36075420202389	24.84859259828748	28.35868802675457	23.431965172934067
50-51	23.183000983979582	25.160184957412135	28.074167461025183	23.5826465975831
52-53	23.293698687002244	24.916207988684235	27.960971372344023	23.829121951969498
54-55	23.282455951539006	25.037571876633557	27.98691614648996	23.693056025337476
56-57	23.524222809876694	24.90025675717229	27.61590587620307	23.959614556747947
58-59	23.326465975831002	24.921973494049997	27.91686525629593	23.834695273823066
60-61	23.44331355124381	24.867585560099627	28.181886319608868	23.507214569047694
62-63	23.10987515759048	24.90755973063559	28.083200086098216	23.899365025675717
64-65	22.965064881153715	24.868066018880107	28.253186402632146	23.91368269733403
66-67	23.339096239247407	24.655979503403447	28.205211986203622	23.799712271145523
68-69	23.216633098613205	24.6535892192737	28.212731773315703	23.917045908797395
70-71	23.091041173395652	24.562205959226347	28.587873989114726	23.758878878263275
72-73	23.23268042188125	24.705382675809474	28.618431167553272	23.443505734756005
74-75	23.230391237661134	24.690332289968794	28.57645650323828	23.502819969131796
76-77	23.110374161920554	24.598755196693343	28.6763271093168	23.614543532069305
78-79	23.16517243698771	24.699088087482103	28.546993763633044	23.588745711897143
80-81	22.892419513545097	24.84240952000246	28.690884351649704	23.57428661480274
82-83	23.094884843639495	24.789366870637433	28.668302788967132	23.447445496755943
84-85	23.026594108145527	24.928659333897393	28.351761402969	23.69298515498808
86-87	22.80897005009141	24.99968710918734	28.545462072338427	23.645880768382817
88-89	23.109987722972487	24.702438554680917	28.39219084759635	23.795382874750246
90-91	23.302557925180736	24.57728713301865	28.2556154857133	23.864539456087318
92-93	23.128144449895625	24.917318403509277	28.180908679884176	23.773628466710917
94-95	22.79248408720519	24.938309092586326	28.252225485071186	24.016981335137295
96-97	22.969773377202422	24.879597029611634	28.02631376648935	24.124315826696595
98-99	22.816507026229203	25.08590602994988	27.741882168445002	24.355704775375912
100-101	22.998985271055624	25.22639217736232	27.573337228252516	24.20128532332954
102-103	23.14677439193137	25.13817994526614	27.705943851665076	24.009101811137416
104-105	23.148556542428324	25.234057968925523	27.58448716729079	24.03289832135537
106-107	23.22140350472588	25.16460834169348	27.570119502004513	24.043868651576126
108-109	23.143701124109448	25.242205322463164	27.294296043034805	24.319797510392583
110-111	22.995592258441444	25.552889535481505	27.193180540594557	24.258337665482493
112-113	23.052220103932843	25.567710095015528	27.113249900064574	24.266819900987056
114-115	22.91857849406777	25.672090410898353	27.143064426701617	24.26626666833226
116-117	23.023821192116618	25.662698069512913	27.125986143542143	24.187494594828333
118-119	22.840433873497126	25.576550536576363	27.200885581624178	24.382130008302326
120-121	22.957531962735366	25.524352217091955	27.27813465428356	24.23998116588912
122-123	22.8581147566188	25.73346837428123	27.124877002552196	24.28353986654777
124-125	22.82179207281449	25.561271947357096	27.21405015220934	24.402885827619077
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	289.0
1	261.5
2	432.5
3	592.0
4	434.0
5	348.0
6	509.0
7	1030.0
8	1695.5
9	2041.5
10	1764.5
11	1307.5
12	1148.0
13	1075.0
14	1078.5
15	1083.5
16	1020.0
17	987.0
18	994.0
19	969.5
20	933.5
21	915.5
22	963.0
23	1010.5
24	1083.0
25	1201.5
26	1372.5
27	1660.0
28	2042.0
29	2744.0
30	3641.0
31	4581.0
32	5715.5
33	6818.5
34	7797.5
35	8733.5
36	9723.0
37	10863.5
38	11352.0
39	11351.5
40	11425.0
41	11535.5
42	11946.5
43	12878.0
44	14280.5
45	15541.5
46	16868.0
47	18201.5
48	19732.0
49	20902.5
50	21413.0
51	21300.5
52	20502.0
53	20192.5
54	19992.0
55	19465.5
56	18727.0
57	17275.5
58	15982.0
59	14712.5
60	13018.5
61	10696.0
62	8294.5
63	6556.5
64	5113.5
65	4162.0
66	3240.0
67	2376.0
68	1904.5
69	1576.5
70	1293.0
71	1104.5
72	934.5
73	910.5
74	740.0
75	568.5
76	474.0
77	330.5
78	222.5
79	163.5
80	124.0
81	81.5
82	59.5
83	39.0
84	25.5
85	12.5
86	6.0
87	5.5
88	3.0
89	1.5
90	1.5
91	1.5
92	0.5
93	0.5
94	1.5
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02594477414593647
2	0.0
3	7.68734048768488E-4
4	0.0
5	0.0019218351219212203
6	7.68734048768488E-4
7	0.0057655053657636604
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.92183512192122E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.001537468097536976
42-43	0.0
44-45	0.0
46-47	0.006053780634051844
48-49	0.009801359121798223
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.01047400141447065
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.07370237692567878
76-77	0.03334383936533317
78-79	1.92183512192122E-4
80-81	0.0
82-83	0.0
84-85	0.05688631960886812
86-87	0.18939685126533626
88-89	0.20630900033824298
90-91	0.12626456751022416
92-93	0.10973678546170167
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.001057009317056671
106-107	0.0019218351219212203
108-109	0.002498385658497586
110-111	2.88275268288183E-4
112-113	0.0
114-115	9.6091756096061E-5
116-117	1.92183512192122E-4
118-119	0.0
120-121	6.726422926724271E-4
122-123	0.0
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	520336.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.7583890325218
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.19679447596683	63.57736700314687
2	9.553693640785093	14.093301045091131
3	2.13756519484935	4.729900956722298
4	0.741299490517201	2.1870822484471164
5	0.3825591976814515	1.4108475065278956
6	0.21486650476431843	0.9508924349078877
7	0.14329856033414984	0.7398629672648565
8	0.0953384346154889	0.5625607480096699
9	0.07920712343841592	0.5257970842245893
>10	0.40468286672825005	5.576721018982203
>50	0.0350961998184689	1.753637079488778
>100	0.014098472952855457	1.7689033658096764
>500	5.999350192704435E-4	0.3166104711036452
>1k	8.999025289056654E-4	1.8065160702734193
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3260	0.6265182497463178	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3212	0.617293441161096	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1693	0.3253666861412626	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	799	0.15355462624150548	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	632	0.1214599797054211	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	3.84367024384244E-4
16-17	0.0	0.0	0.0	2.88275268288183E-4	3.84367024384244E-4
18-19	0.0	0.0	0.0	3.84367024384244E-4	3.84367024384244E-4
20-21	0.0	0.0	0.0	8.64825804864549E-4	3.84367024384244E-4
22-23	0.0	0.0	0.0	0.004228037268226684	3.84367024384244E-4
24-25	0.0	0.0	0.0	0.008071707512069124	3.84367024384244E-4
26-27	0.0	0.0	0.0	0.011723194243719443	3.84367024384244E-4
28-29	0.0	0.0	0.0	0.0248877648288798	3.84367024384244E-4
30-31	0.0	0.0	0.0	0.045067033609052606	3.84367024384244E-4
32-33	0.0	0.0	0.0	0.07783432243780941	3.84367024384244E-4
34-35	0.0	0.0	0.0	0.11598274960794563	3.84367024384244E-4
36-37	0.0	0.0	0.0	0.17690492297284832	3.84367024384244E-4
38-39	0.0	0.0	0.0	0.2615617600934781	3.84367024384244E-4
40-41	0.0	0.0	0.0	0.346602964238492	3.84367024384244E-4
42-43	0.0	0.0	0.0	0.433085544724947	3.84367024384244E-4
44-45	0.0	0.0	0.0	0.5206251345284585	3.84367024384244E-4
46-47	0.0	0.0	0.0	0.6213292949171305	3.84367024384244E-4
48-49	0.0	0.0	0.0	0.73087389686664	3.84367024384244E-4
50-51	0.0	0.0	0.0	0.8311936902309277	3.84367024384244E-4
52-53	0.0	0.0	0.0	0.9272854463269887	3.84367024384244E-4
54-55	0.0	0.0	0.0	1.0266443221303159	3.84367024384244E-4
56-57	0.0	0.0	0.0	1.13753420866517	3.84367024384244E-4
58-59	0.0	0.0	0.0	1.2639909596875865	3.84367024384244E-4
60-61	0.0	0.0	0.0	1.3854509393930077	3.84367024384244E-4
62-63	0.0	0.0	0.0	1.4973978352449187	3.84367024384244E-4
64-65	0.0	0.0	0.0	1.6014652070969526	3.84367024384244E-4
66-67	0.0	0.0	0.0	1.7301320685095785	3.84367024384244E-4
68-69	0.0	0.0	0.0	1.8661019033855046	3.84367024384244E-4
70-71	0.0	0.0	0.0	2.011680913871037	3.84367024384244E-4
72-73	0.0	0.0	0.0	2.153608437624919	3.84367024384244E-4
74-75	0.0	0.0	0.0	2.279488638110759	3.84367024384244E-4
76-77	0.0	0.0	0.0	2.421896620645122	3.84367024384244E-4
78-79	0.0	0.0	0.0	2.59245948771563	3.84367024384244E-4
80-81	0.0	0.0	0.0	2.7624458042495617	3.84367024384244E-4
82-83	0.0	0.0	0.0	2.9335852218566463	3.84367024384244E-4
84-85	0.0	0.0	0.0	3.0978060330248147	3.84367024384244E-4
86-87	0.0	0.0	0.0	3.2676001660465546	3.84367024384244E-4
88-89	0.0	0.0	0.0	3.4399887764828883	3.84367024384244E-4
90-91	0.0	0.0	0.0	3.6422619230650963	3.84367024384244E-4
92-93	0.0	9.6091756096061E-5	0.0	3.8349258940376987	3.84367024384244E-4
94-95	0.0	1.92183512192122E-4	0.0	4.034796746717506	3.84367024384244E-4
96-97	0.0	1.92183512192122E-4	0.0	4.240048737738691	3.84367024384244E-4
98-99	0.0	1.92183512192122E-4	0.0	4.456735647735309	3.84367024384244E-4
100-101	0.0	1.92183512192122E-4	0.0	4.70042434119492	3.84367024384244E-4
102-103	0.0	1.92183512192122E-4	0.0	4.930660188801083	3.84367024384244E-4
104-105	0.0	1.92183512192122E-4	0.0	5.1681029181144496	3.84367024384244E-4
106-107	0.0	1.92183512192122E-4	0.0	5.421112511915378	3.84367024384244E-4
108-109	0.0	1.92183512192122E-4	0.0	5.675659573813844	3.84367024384244E-4
110-111	0.0	1.92183512192122E-4	0.0	5.957592786199687	3.84367024384244E-4
112-113	0.0	1.92183512192122E-4	0.0	6.2401986408782015	3.84367024384244E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCAACG	155	0.0	57.559578	1
GGTATCA	2630	0.0	50.658264	1
GTATCAA	3900	0.0	35.381924	1
TAGGACG	70	0.002792595	33.98756	4
ATCAACG	4820	0.0	28.628525	3
TATCAAC	4905	0.0	28.374937	2
TCAACGC	4945	0.0	27.904854	4
CAACGCA	4985	0.0	27.800259	5
AACGCAG	5185	0.0	26.727922	6
CATGGGT	1400	0.0	26.34036	4
GAGTACT	2525	0.0	25.084448	12-13
ACGCAGA	5550	0.0	24.967741	7
CGCAGAG	5705	0.0	24.28939	8
GTACTAG	180	2.1802467E-5	23.130423	1
ATGGGTG	675	0.0	22.910133	5
GCAGAGT	6045	0.0	22.824852	9
CAGAGTA	6015	0.0	22.69151	10-11
ACATGGG	4885	0.0	22.646778	3
GTACATG	5045	0.0	22.518023	1
GTATTAT	160	2.6150665E-4	22.304337	1
>>END_MODULE