ERR1142497_2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1142497_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	93364
Sequences flagged as poor quality	0
Sequence length	125
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	436	0.4669894177627351	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	367	0.3930851291718435	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	250	0.2677691615612013	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	247	0.2645559316224669	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	244	0.26134270168373247	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	237	0.25384516516001887	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	217	0.23242363223512277	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	197	0.21100209931022665	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	194	0.2077888693714922	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	187	0.2002913328477786	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	179	0.19172271967782015	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	179	0.19172271967782015	No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC	176	0.18850948973908574	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	172	0.1842251831541065	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	168	0.1799408765691273	No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG	166	0.17779872327663768	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	161	0.17244334004541365	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	156	0.16708795681418961	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	154	0.16494580352170002	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	149	0.159590420290476	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	149	0.159590420290476	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	146	0.15637719035174158	No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT	144	0.15423503705925196	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	142	0.15209288376676236	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	142	0.15209288376676236	No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC	138	0.14780857718178314	No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	128	0.13709781071933508	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	123	0.13174242748811105	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	122	0.13067135084186624	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	118	0.12638704425688702	No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA	117	0.1253159676106422	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	116	0.1242448909643974	No Hit
GGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGA	116	0.1242448909643974	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	114	0.1221027376719078	No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA	113	0.12103166102566298	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	113	0.12103166102566298	No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC	110	0.11781843108692859	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	109	0.11674735444068378	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	101	0.10817874127072534	No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG	100	0.10710766462448053	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	99	0.10603658797823572	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	99	0.10603658797823572	No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA	98	0.10496551133199093	No Hit
GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT	96	0.1028233580395013	No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA	96	0.1028233580395013	No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA	96	0.1028233580395013	No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC	96	0.1028233580395013	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	95	0.10175228139325651	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	94	0.1006812047470117	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAA	50	9.094947E-12	95.30445	1
ATTCAAG	20	8.420163E-4	89.34793	1
ACGTTAA	20	8.4381684E-4	89.29994	8
TACAAGA	20	8.4381684E-4	89.29994	2
TCAAGAC	20	8.456203E-4	89.252014	3
GTACATA	25	0.0020507171	71.4016	3
TAACCAC	25	0.0020507171	71.4016	6
GTACAAG	35	9.059065E-5	68.07461	1
TTCTCTG	95	0.0	62.666626	7
CGTTAAC	30	0.004206038	59.56529	9
TACAAAG	30	0.004215002	59.5333	2
TATTCTC	100	1.8189894E-12	59.50134	5
CCTCTTC	15	0.004230612	59.50134	64-65
TCTCTGC	105	1.8189894E-12	56.69838	8
GAACAAA	75	1.4452125E-6	47.65223	1
CTCTGCA	140	3.8198777E-11	42.54663	9
ACTGTGC	70	5.395479E-5	42.52378	8
TAGTACT	70	5.4126194E-5	42.500954	4
TACTGTG	85	3.4331915E-6	42.023506	7
GCATATA	60	0.0012953249	39.710194	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content