FastQCFastQC Report
Sun 1 Jan 2017
ERR1142496_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142496_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180270
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4030.2235535585510623No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3140.17418316968990957No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2050.11371831142175627No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1900.1053974593665058No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTGT150.004227324459.52436422-23
GTATATC600.001299376139.7050254
GCTAACG350.002792268833.9855362-63
CAGGGTA750.00388335631.7640174
ATGGGGG4350.030.1210545
GATACTA400.005383142729.720802100-101
ACAGGGT1055.852898E-428.3607313
TAGGACT1107.682124E-427.0716084
GTACAAA1556.7094225E-626.9119511
CATGGGG7150.026.6551234
CATGGGT2109.6248186E-825.5246584
GGTATCA10850.024.7150551
TATAAGC1451.3252065E-424.6444972
TGGGGGG2352.8844806E-722.7838766
ATATAAG1852.6428681E-522.5478481
GTGTTGG1602.5727053E-422.3465291
GTAAAAA1602.5727053E-422.3465291
GTACATG18800.021.5540981
GGTTAAT1400.003117734821.282411
TACATGG19400.020.8758372
Produced by FastQC (version 0.11.5)