##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142496_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	180270
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.78766849725412	33.0	33.0	33.0	27.0	33.0
2	30.880518111721308	33.0	33.0	33.0	27.0	33.0
3	30.99760914184279	33.0	33.0	33.0	27.0	33.0
4	30.90360015532257	33.0	33.0	33.0	27.0	33.0
5	31.04261385699229	33.0	33.0	33.0	27.0	33.0
6	34.27049425861208	37.0	37.0	37.0	27.0	37.0
7	34.30660120929716	37.0	37.0	37.0	27.0	37.0
8	34.200072114051146	37.0	37.0	37.0	27.0	37.0
9	34.05699783657847	37.0	37.0	37.0	27.0	37.0
10-11	34.26093637321795	37.0	37.0	37.0	27.0	37.0
12-13	34.201004049481334	37.0	37.0	37.0	27.0	37.0
14-15	34.141529372607756	37.0	37.0	37.0	27.0	37.0
16-17	34.15709213956842	37.0	37.0	37.0	27.0	37.0
18-19	34.19157929772008	37.0	37.0	37.0	27.0	37.0
20-21	34.168186608975425	37.0	37.0	37.0	27.0	37.0
22-23	34.299597825484	37.0	37.0	37.0	27.0	37.0
24-25	34.44030343373828	37.0	37.0	37.0	27.0	37.0
26-27	34.51316636156876	37.0	37.0	37.0	27.0	37.0
28-29	34.5808980972985	37.0	37.0	37.0	27.0	37.0
30-31	34.55875076274477	37.0	37.0	37.0	27.0	37.0
32-33	34.567337882065786	37.0	37.0	37.0	27.0	37.0
34-35	34.63071503855328	37.0	37.0	37.0	27.0	37.0
36-37	34.61002662672658	37.0	37.0	37.0	27.0	37.0
38-39	34.5873190214678	37.0	37.0	37.0	27.0	37.0
40-41	34.55007211405115	37.0	37.0	37.0	27.0	37.0
42-43	34.54036722693738	37.0	37.0	37.0	27.0	37.0
44-45	34.507172574471625	37.0	37.0	37.0	27.0	37.0
46-47	34.49001220391635	37.0	37.0	37.0	27.0	37.0
48-49	34.460317856548514	37.0	37.0	37.0	27.0	37.0
50-51	34.37274920951906	37.0	37.0	37.0	27.0	37.0
52-53	34.3673489765352	37.0	37.0	37.0	27.0	37.0
54-55	34.32380595773007	37.0	37.0	37.0	27.0	37.0
56-57	34.28204637488212	37.0	37.0	37.0	27.0	37.0
58-59	34.223780995173904	37.0	37.0	37.0	27.0	37.0
60-61	34.19051145503966	37.0	37.0	37.0	27.0	37.0
62-63	34.13713318910523	37.0	37.0	37.0	27.0	37.0
64-65	34.09778942697065	37.0	37.0	37.0	27.0	37.0
66-67	34.044732900649024	37.0	37.0	37.0	27.0	37.0
68-69	34.00052698729684	37.0	37.0	37.0	27.0	37.0
70-71	33.929661063959614	37.0	37.0	37.0	27.0	37.0
72-73	33.85922782492927	37.0	37.0	37.0	22.0	37.0
74-75	33.77958062905641	37.0	37.0	37.0	22.0	37.0
76-77	33.72507072724247	37.0	37.0	37.0	22.0	37.0
78-79	33.67907305708104	37.0	37.0	37.0	22.0	37.0
80-81	33.61940422699284	37.0	37.0	37.0	14.0	37.0
82-83	33.57310145892272	37.0	37.0	37.0	14.0	37.0
84-85	33.49015920563599	37.0	35.0	37.0	14.0	37.0
86-87	33.416880235202754	37.0	33.0	37.0	14.0	37.0
88-89	33.35915016364342	37.0	33.0	37.0	14.0	37.0
90-91	33.26746824208132	37.0	33.0	37.0	14.0	37.0
92-93	33.186572918400174	37.0	33.0	37.0	14.0	37.0
94-95	33.13490042713707	37.0	33.0	37.0	14.0	37.0
96-97	33.046846397071064	37.0	33.0	37.0	14.0	37.0
98-99	32.95931658068453	37.0	33.0	37.0	14.0	37.0
100-101	32.87782492927276	37.0	33.0	37.0	14.0	37.0
102-103	32.77007266877462	37.0	33.0	37.0	14.0	37.0
104-105	32.64012037499306	37.0	33.0	37.0	14.0	37.0
106-107	32.56996727131525	37.0	33.0	37.0	14.0	37.0
108-109	32.47032229433627	37.0	33.0	37.0	14.0	37.0
110-111	32.36946524657458	37.0	33.0	37.0	14.0	37.0
112-113	32.24551228712487	37.0	33.0	37.0	14.0	37.0
114-115	32.14839130193599	37.0	33.0	37.0	14.0	37.0
116-117	31.925686470294558	37.0	33.0	37.0	14.0	37.0
118-119	31.838031841127197	37.0	30.0	37.0	14.0	37.0
120-121	31.711335774116602	37.0	27.0	37.0	14.0	37.0
122-123	31.647032229433627	37.0	27.0	37.0	14.0	37.0
124-125	30.104190935818494	35.0	24.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	365.0
1	17.0
2	7.0
3	12.0
4	9.0
5	10.0
6	6.0
7	10.0
8	11.0
9	6.0
10	2.0
11	4.0
12	3.0
13	5.0
14	25.0
15	109.0
16	183.0
17	357.0
18	750.0
19	1547.0
20	2423.0
21	2800.0
22	2534.0
23	2168.0
24	2017.0
25	2202.0
26	2509.0
27	2922.0
28	3410.0
29	4185.0
30	4733.0
31	5946.0
32	7182.0
33	9654.0
34	14620.0
35	27184.0
36	80343.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.69819518716578	20.48852495543672	12.04879679144385	23.764483065953655
2	17.046023986369864	24.29148895892028	35.44838030756896	23.2141067471409
3	19.2896509491733	29.00573400879586	27.66241719089239	24.042197851138454
4	13.989167163032526	20.771658715535047	33.6658520699848	31.573322051447626
5	14.542002371977262	38.95443687812158	30.792273813036967	15.711286936864202
6	28.525004312527475	37.58743301671035	17.801556961532256	16.086005709229923
7	25.3164627394684	35.3086172456196	20.99365127058051	18.381268744331493
8	24.903048478542694	35.625858932058826	19.63233534933149	19.83875724006699
9	25.539740479423088	20.25807384985199	19.40227915155022	34.7999065191747
10-11	24.274283742954278	29.035049160077676	24.889132479036718	21.801534617931324
12-13	25.10589269910889	28.156882607993854	24.655326917617984	22.081897775279273
14-15	23.920609904827668	28.013187131409094	22.899275487417093	25.16692747634614
16-17	23.215672520479995	29.702465950179956	23.873704603719386	23.208156925620663
18-19	23.449623189696435	28.475894159162003	25.568833279529795	22.505649371611767
20-21	25.55242354001853	27.172198090552662	24.875129044914587	22.40024932451422
22-23	25.478580729891263	26.960789769502163	24.445458491469022	23.11517100913755
24-25	24.250829012084658	27.73184519172991	24.85589655709612	23.16142923908931
26-27	23.96915175677518	27.434293075003406	24.972387372474174	23.62416779574724
28-29	23.89357144844085	27.325377618292578	25.334223483267966	23.446827449998608
30-31	24.24821546798413	27.24728634297509	25.16231869544172	23.34217949359905
32-33	23.551861494981864	27.645648352137435	25.057858780069875	23.744631372810822
34-35	23.750229456036223	27.67990743882563	25.140872099814764	23.42899100532338
36-37	23.589426925569583	27.90104501317045	24.948750684946745	23.56077737631322
38-39	23.70248614251585	27.93357307545758	24.805244289437162	23.55869649258941
40-41	23.340333504860716	27.955439967734307	25.119675117867125	23.58455140953785
42-43	23.49983173083609	27.923781022019618	24.842091210230766	23.734296036913527
44-45	23.527906640412063	28.07351288811757	24.847311684410773	23.551268787059595
46-47	23.473346664404648	28.03335178175609	24.863375060142783	23.62992649369648
48-49	23.267461650555635	28.14220719267606	25.013070514032727	23.577260642735574
50-51	22.88010679422644	28.433128458992684	24.875545790805685	23.811218955975193
52-53	22.998153339563032	28.12764205936012	24.607306545632536	24.26689805544431
54-55	22.947856147601396	27.921115353915727	24.69024899669877	24.44077950178411
56-57	23.206329782796118	27.265344717301222	24.67975637567094	24.84856912423172
58-59	23.129847629059217	27.39339735193266	24.988945528570593	24.487809490437535
60-61	23.10806827472289	27.421956252120378	24.979004799697446	24.490970673459287
62-63	22.700816195998275	27.41973609199238	24.902737802249753	24.976709909759588
64-65	22.714354836018618	27.062072110651975	25.032673922594782	25.19089913073462
66-67	23.134826399123515	26.795655389885937	24.863883342880502	25.20563486811005
68-69	23.062654690063685	26.59334241775355	24.850802302622434	25.493200589560335
70-71	22.993239654390887	26.547496224940698	24.944868839283977	25.514395281384438
72-73	23.259202411340418	26.49805079608713	25.123041759121772	25.119705033450675
74-75	23.278166046950133	26.314823415551224	25.230481710250768	25.176528827247875
76-77	23.140254502468537	26.084695083791114	25.46554481607677	25.309505597663584
78-79	23.04492072894332	26.085336314514628	25.586711495624172	25.283031460917883
80-81	22.79412173700224	26.241093330218433	25.765514709563302	25.199270223216025
82-83	23.359085170167916	26.02798774145823	25.696773511760746	24.916153576613105
84-85	23.282726167863014	26.30858782925018	25.272341455139095	25.136344547747708
86-87	23.006654449693972	26.492384784585482	25.43957464913281	25.061386116587737
88-89	23.344668711298212	25.8808089177819	25.55520644197964	25.21931592894025
90-91	23.432525836111335	25.99041902182828	25.470499428644512	25.106555713415872
92-93	23.209678136886357	26.626276219498635	25.34838846008408	24.815657183530927
94-95	22.504879310057216	26.715265150883283	25.642372985025663	25.13748255403384
96-97	22.80905623450132	26.81953139768924	25.27188719739344	25.099525170416005
98-99	22.534118804889644	27.466576220673396	24.995899349178064	25.003405625258896
100-101	22.607895570763816	27.82240803233392	24.932313354422288	24.63738304247997
102-103	22.53016837581415	28.035949084176785	25.00340532231028	24.430477217698783
104-105	22.621556253648773	28.265269244669316	24.811931833977372	24.30124266770454
106-107	22.377989792174	28.449589129443687	24.907429028922174	24.26499204946014
108-109	22.520278647237713	28.70978323112932	24.496995035275674	24.272943086357294
110-111	22.275967130355497	29.117323281182237	24.465145500439224	24.141564088023042
112-113	22.318834939581535	29.143189127733564	24.420346860658086	24.11762907202682
114-115	22.315675952125503	29.14079394286413	24.48120208797745	24.062328017032915
116-117	22.39002207063716	29.114617210649502	24.406121961117876	24.089238757595467
118-119	22.23266052597933	28.6734336522266	24.705519392083072	24.388386429710994
120-121	22.32157250779639	28.833015548304253	24.854217276017142	23.99119466788222
122-123	22.159596229099964	29.004079953753113	24.867151369619354	23.96917244752757
124-125	22.329128418056957	28.904971138106315	24.61028800751493	24.155612436321796
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	539.0
1	309.0
2	73.5
3	61.5
4	49.0
5	42.0
6	43.0
7	38.5
8	32.5
9	30.0
10	30.5
11	41.0
12	39.0
13	34.5
14	46.0
15	61.0
16	70.5
17	77.5
18	94.0
19	120.0
20	140.5
21	165.0
22	213.0
23	265.5
24	333.0
25	439.5
26	556.0
27	682.0
28	858.0
29	1163.5
30	1502.5
31	1790.5
32	2161.0
33	2568.0
34	2922.0
35	3280.0
36	3618.0
37	4034.0
38	4328.0
39	4355.5
40	4379.0
41	4501.0
42	4621.0
43	4932.5
44	5450.0
45	5937.0
46	6409.5
47	6793.5
48	7254.5
49	7619.5
50	7906.5
51	7918.5
52	7620.5
53	7380.5
54	6940.5
55	6487.0
56	6176.0
57	5588.0
58	4930.0
59	4446.0
60	3854.0
61	3226.5
62	2648.0
63	2066.5
64	1583.0
65	1270.5
66	986.5
67	753.0
68	651.5
69	542.0
70	437.0
71	383.5
72	343.5
73	307.0
74	253.5
75	217.0
76	172.5
77	110.5
78	80.5
79	52.5
80	26.0
81	23.0
82	20.5
83	15.0
84	7.0
85	3.5
86	2.0
87	1.0
88	1.0
89	0.5
90	0.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.41604260276252286
2	0.3705552781938204
3	0.3550230210240195
4	0.35003050979086925
5	0.3733288955455705
6	0.31119986686636714
7	0.3045431852221667
8	0.3001053974593665
9	0.3073168025739169
10-11	0.3056526321628668
12-13	0.33671714650246853
14-15	0.3888611527153714
16-17	0.3569645531702446
18-19	0.33505297609141843
20-21	0.32423586841959284
22-23	0.3173018250402174
24-25	0.29844122704831644
26-27	0.3059299938980418
28-29	0.291229821933766
30-31	0.2945581627558662
32-33	0.28956565152271596
34-35	0.2756975647639652
36-37	0.2837410550840406
38-39	0.27375603261774006
40-41	0.28429577855439064
42-43	0.2765296499694902
44-45	0.272923947412215
46-47	0.27153713873633994
48-49	0.2640483718866145
50-51	0.2690408831197648
52-53	0.26959560659011483
54-55	0.27098241526598993
56-57	0.2690408831197648
58-59	0.2654351805624896
60-61	0.25905586065346425
62-63	0.26210683974038945
64-65	0.25683696677206413
66-67	0.25572751983136405
68-69	0.2634936484162645
70-71	0.2615521162700394
72-73	0.2512897320685638
74-75	0.26820879791423974
76-77	0.2815221612026405
78-79	0.2637710101514395
80-81	0.27125977700116494
82-83	0.2646030953569646
84-85	0.26987296832528984
86-87	0.2582237754479392
88-89	0.24934819992233873
90-91	0.24102734786708827
92-93	0.24518777389471347
94-95	0.23797636878016307
96-97	0.2307649636656127
98-99	0.23326121928218782
100-101	0.22022521772896209
102-103	0.22438564375658734
104-105	0.23021024019526265
106-107	0.22632717590281246
108-109	0.22244411161036226
110-111	0.22632717590281246
112-113	0.22161202640483718
114-115	0.2124590891440617
116-117	0.21800632384756197
118-119	0.20913074832196152
120-121	0.21024019526266155
122-123	0.20302879014811115
124-125	0.20053253453153602
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	180270.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.43264718016205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.74951261234548	58.282672261557
2	11.768742554478438	16.57807384187478
3	2.5048741903848235	5.292747602462051
4	0.9613524359668923	2.708423877529819
5	0.4741979482955322	1.6699508392927978
6	0.2715069746197637	1.1473772970208216
7	0.23377837202902574	1.1525940716831127
8	0.1664039181125265	0.9376214763052926
9	0.11885707029753009	0.7534276287420291
>10	0.7101647977535647	8.854788490466547
>50	0.033675860350223186	1.5870667583731377
>100	0.0069332653662224205	1.0352558546926314
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	403	0.2235535585510623	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	314	0.17418316968990957	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	205	0.11371831142175627	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	190	0.1053974593665058	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	5.547234703500305E-4	0.0
4	0.0	0.0	0.0	5.547234703500305E-4	0.0
5	0.0	0.0	0.0	5.547234703500305E-4	0.0
6	0.0	0.0	0.0	5.547234703500305E-4	0.0
7	0.0	0.0	0.0	5.547234703500305E-4	0.0
8	0.0	0.0	0.0	5.547234703500305E-4	0.0
9	0.0	0.0	0.0	5.547234703500305E-4	0.0
10-11	0.0	0.0	0.0	8.320852055250458E-4	0.0
12-13	0.0	0.0	0.0	0.0019415321462251068	0.0
14-15	0.0	0.0	0.0	0.002218893881400122	0.0
16-17	0.0	0.0	0.0	0.0027736173517501524	0.0
18-19	0.0	0.0	0.0	0.0027736173517501524	5.547234703500305E-4
20-21	0.0	0.0	0.0	0.0030509790869251677	5.547234703500305E-4
22-23	0.0	0.0	0.0	0.006101958173850336	5.547234703500305E-4
24-25	0.0	0.0	0.0	0.017473789316025962	5.547234703500305E-4
26-27	0.0	0.0	0.0	0.02940034392855162	5.547234703500305E-4
28-29	0.0	0.0	0.0	0.05935541132745326	5.547234703500305E-4
30-31	0.0	0.0	0.0	0.11177677927553115	5.547234703500305E-4
32-33	0.0	0.0	0.0	0.1780662339823598	5.547234703500305E-4
34-35	0.0	0.0	0.0	0.2504576468630388	5.547234703500305E-4
36-37	0.0	0.0	0.0	0.3428191046763188	5.547234703500305E-4
38-39	0.0	0.0	0.0	0.45931103344982527	5.547234703500305E-4
40-41	0.0	0.0	0.0	0.599656071448383	5.547234703500305E-4
42-43	0.0	0.0	0.0	0.7369501303600156	5.547234703500305E-4
44-45	0.0	0.0	0.0	0.8606534642480723	5.547234703500305E-4
46-47	0.0	0.0	0.0	0.9807510955788539	5.547234703500305E-4
48-49	0.0	0.0	0.0	1.1044544294669107	0.0013868086758750764
50-51	0.0	0.0	0.0	1.2115160592444667	0.002218893881400122
52-53	0.0	0.0	0.0	1.3302268818993732	0.002218893881400122
54-55	0.0	0.0	0.0	1.436179064736229	0.002218893881400122
56-57	0.0	0.0	0.0	1.5546125256559606	0.002218893881400122
58-59	0.0	0.0	0.0	1.6846951794530427	0.002218893881400122
60-61	0.0	0.0	0.0	1.8045154490486492	0.002218893881400122
62-63	0.0	0.0	0.0	1.9149054196483053	0.002218893881400122
64-65	0.0	0.0	0.0	2.0222444111610365	0.002218893881400122
66-67	0.0	0.0	0.0	2.142619404226993	0.002218893881400122
68-69	0.0	0.0	0.0	2.273811504964775	0.002218893881400122
70-71	0.0	0.0	0.0	2.4241415654296334	0.002218893881400122
72-73	0.0	0.0	0.0	2.5619903478116157	0.002218893881400122
74-75	0.0	0.0	0.0	2.684861596494148	0.002218893881400122
76-77	0.0	0.0	0.0	2.815221612026405	0.002218893881400122
78-79	0.0	0.0	0.0	2.964164863815388	0.002218893881400122
80-81	0.0	0.0	0.0	3.1230931380706717	0.002218893881400122
82-83	0.0	0.0	0.0	3.2825761357963055	0.002218893881400122
84-85	0.0	0.0	0.0	3.4484384534309647	0.002218893881400122
86-87	0.0	0.0	0.0	3.607089365951073	0.002218893881400122
88-89	0.0	0.0	0.0	3.7696233427636323	0.002218893881400122
90-91	0.0	0.0	0.0	3.944915959394242	0.002218893881400122
92-93	0.0	0.0	0.0	4.115770788262051	0.002218893881400122
94-95	0.0	0.0	0.0	4.289676596216786	0.002218893881400122
96-97	0.0	0.0	0.0	4.481333555222721	0.002218893881400122
98-99	0.0	0.0	0.0	4.669662173406557	0.002218893881400122
100-101	0.0	0.0	0.0	4.884894879902369	0.002218893881400122
102-103	0.0	0.0	0.0	5.0976313307816055	0.002218893881400122
104-105	0.0	0.0	0.0	5.310090419925666	0.002218893881400122
106-107	0.0	0.0	0.0	5.532811893271204	0.002218893881400122
108-109	0.0	0.0	0.0	5.761357963055417	0.002218893881400122
110-111	0.0	0.0	0.0	5.981305819049204	0.002218893881400122
112-113	0.0	0.0	0.0	6.241748488378544	0.002218893881400122
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTGT	15	0.0042273244	59.524364	22-23
GTATATC	60	0.0012993761	39.705025	4
GCTAACG	35	0.0027922688	33.98553	62-63
CAGGGTA	75	0.003883356	31.764017	4
ATGGGGG	435	0.0	30.121054	5
GATACTA	40	0.0053831427	29.720802	100-101
ACAGGGT	105	5.852898E-4	28.360731	3
TAGGACT	110	7.682124E-4	27.071608	4
GTACAAA	155	6.7094225E-6	26.911951	1
CATGGGG	715	0.0	26.655123	4
CATGGGT	210	9.6248186E-8	25.524658	4
GGTATCA	1085	0.0	24.715055	1
TATAAGC	145	1.3252065E-4	24.644497	2
TGGGGGG	235	2.8844806E-7	22.783876	6
ATATAAG	185	2.6428681E-5	22.547848	1
GTGTTGG	160	2.5727053E-4	22.346529	1
GTAAAAA	160	2.5727053E-4	22.346529	1
GTACATG	1880	0.0	21.554098	1
GGTTAAT	140	0.0031177348	21.28241	1
TACATGG	1940	0.0	20.875837	2
>>END_MODULE