FastQCFastQC Report
Sun 1 Jan 2017
ERR1142496_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142496_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180270
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13240.7344538747434404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13170.7305708104509901No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6810.37776668330837077No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.19027015033006048No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3080.1708548288678094No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2090.11593720530315638No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2060.11427303489210629No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1850.10262384201475563No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGTA150.00423104959.51116668-69
GGTATCA14650.041.8464241
GCGCAGA758.1454804E-539.6796151
CTGACCG700.002785901533.9922339
TATGACT750.003906186231.7260824
TAGTAGT750.003906186231.7260824
GTATCAA19950.030.712282
CGCATAA703.0921492E-629.743204106-107
ATCAACG22000.028.1208484
CAACGCA22550.027.698776
TATCAAC22600.027.637493
TCAACGC22650.027.5764795
ATGGGGG4900.026.7081855
AACGCAG23400.026.692627
ACGCAGA23950.026.0796388
CATGGGG7200.025.6122044
CGCAGAG24900.025.0846319
GAGTACT13100.024.74816112-13
CAGAGTA26150.023.20311210-11
GCAGAGT26400.022.532739
Produced by FastQC (version 0.11.5)