##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142496_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	180270
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.19553447606368	27.0	14.0	33.0	14.0	33.0
2	28.78838409053087	33.0	27.0	33.0	14.0	33.0
3	31.278853941310256	33.0	33.0	33.0	27.0	33.0
4	30.959965607144838	33.0	33.0	33.0	27.0	33.0
5	32.05976590669551	33.0	33.0	33.0	33.0	33.0
6	35.06427580850946	37.0	37.0	37.0	33.0	37.0
7	35.63028235424641	37.0	37.0	37.0	33.0	37.0
8	35.510789371498305	37.0	37.0	37.0	33.0	37.0
9	35.906723248460644	37.0	37.0	37.0	33.0	37.0
10-11	35.994164309091914	37.0	37.0	37.0	33.0	37.0
12-13	36.06151605924447	37.0	37.0	37.0	37.0	37.0
14-15	36.0182975536695	37.0	37.0	37.0	33.0	37.0
16-17	36.01237588062351	37.0	37.0	37.0	33.0	37.0
18-19	36.01226493592944	37.0	37.0	37.0	33.0	37.0
20-21	36.02675153935763	37.0	37.0	37.0	35.0	37.0
22-23	36.02444388972097	37.0	37.0	37.0	37.0	37.0
24-25	36.007577522604976	37.0	37.0	37.0	35.0	37.0
26-27	35.95230210240196	37.0	37.0	37.0	33.0	37.0
28-29	35.90231874410607	37.0	37.0	37.0	33.0	37.0
30-31	35.80630443224052	37.0	37.0	37.0	33.0	37.0
32-33	35.73870305652632	37.0	37.0	37.0	33.0	37.0
34-35	35.60660953014922	37.0	37.0	37.0	33.0	37.0
36-37	35.49022854606979	37.0	37.0	37.0	33.0	37.0
38-39	35.39980307316803	37.0	37.0	37.0	33.0	37.0
40-41	35.22816885782437	37.0	37.0	37.0	33.0	37.0
42-43	35.1306623398236	37.0	37.0	37.0	33.0	37.0
44-45	34.98881122760304	37.0	37.0	37.0	30.0	37.0
46-47	34.75667609696566	37.0	37.0	37.0	27.0	37.0
48-49	34.54995562212237	37.0	37.0	37.0	27.0	37.0
50-51	34.38979863538026	37.0	37.0	37.0	27.0	37.0
52-53	34.304310201364615	37.0	37.0	37.0	27.0	37.0
54-55	34.22865146724358	37.0	37.0	37.0	27.0	37.0
56-57	34.167715094025624	37.0	37.0	37.0	27.0	37.0
58-59	34.099403672269375	37.0	37.0	37.0	27.0	37.0
60-61	34.074155433516395	37.0	37.0	37.0	27.0	37.0
62-63	34.0608587119321	37.0	37.0	37.0	27.0	37.0
64-65	34.04811116658346	37.0	37.0	37.0	27.0	37.0
66-67	34.014575359183446	37.0	37.0	37.0	22.0	37.0
68-69	33.97153991235369	37.0	37.0	37.0	22.0	37.0
70-71	33.926146890774945	37.0	37.0	37.0	22.0	37.0
72-73	33.90986020968547	37.0	37.0	37.0	22.0	37.0
74-75	33.80757752260498	37.0	37.0	37.0	22.0	37.0
76-77	33.75621845010262	37.0	37.0	37.0	22.0	37.0
78-79	33.72311532700948	37.0	37.0	37.0	22.0	37.0
80-81	33.68789593387696	37.0	37.0	37.0	22.0	37.0
82-83	33.62267432185055	37.0	35.0	37.0	14.0	37.0
84-85	33.542733122538415	37.0	33.0	37.0	14.0	37.0
86-87	33.36121928218783	37.0	33.0	37.0	14.0	37.0
88-89	33.28615132856271	37.0	33.0	37.0	14.0	37.0
90-91	33.21979530703944	37.0	33.0	37.0	14.0	37.0
92-93	33.1497697897598	37.0	33.0	37.0	14.0	37.0
94-95	33.14671048982082	37.0	33.0	37.0	14.0	37.0
96-97	33.107854884340156	37.0	33.0	37.0	14.0	37.0
98-99	33.01740999611694	37.0	33.0	37.0	14.0	37.0
100-101	32.94762855716425	37.0	33.0	37.0	14.0	37.0
102-103	32.84321850557497	37.0	33.0	37.0	14.0	37.0
104-105	32.73513341099462	37.0	33.0	37.0	14.0	37.0
106-107	32.66620069895157	37.0	33.0	37.0	14.0	37.0
108-109	32.56581239252233	37.0	33.0	37.0	14.0	37.0
110-111	32.46638375769679	37.0	33.0	37.0	14.0	37.0
112-113	32.420280690076	37.0	33.0	37.0	14.0	37.0
114-115	32.29130193598491	37.0	33.0	37.0	14.0	37.0
116-117	32.15055472347035	37.0	33.0	37.0	14.0	37.0
118-119	31.986744882675985	37.0	33.0	37.0	14.0	37.0
120-121	31.89323514727908	37.0	33.0	37.0	14.0	37.0
122-123	31.75450435457924	37.0	27.0	37.0	14.0	37.0
124-125	30.54032839629445	35.0	24.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	7.0
15	100.0
16	37.0
17	15.0
18	46.0
19	232.0
20	797.0
21	2027.0
22	3133.0
23	4037.0
24	3246.0
25	2031.0
26	1743.0
27	2016.0
28	2354.0
29	3099.0
30	3892.0
31	5305.0
32	7381.0
33	10617.0
34	17448.0
35	34426.0
36	76281.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.18122294972811	14.216513150593718	12.220064365775164	21.382199533903005
2	18.262051367393354	20.948577134298553	37.98690852609974	22.802462972208353
3	20.36167970266822	27.429966161868307	28.681977034448323	23.526377101015143
4	14.269151827813834	18.810118156099183	34.5803516946802	32.340378321406774
5	15.600019970155712	35.244551198486704	32.78877677249	16.36665205886758
6	28.853077413807448	34.67894488669459	19.163453804121712	17.30452389537625
7	25.328555023604927	32.742331866924815	23.331428666529828	18.597684442940434
8	25.782992178399066	31.666389304931492	21.673600710046042	20.8770178066234
9	26.663338325844567	16.099184556498585	21.615909469129637	35.62156764852721
10-11	25.129527930326734	25.31452820768847	27.000332834082208	22.555611027902593
12-13	26.495811837798854	24.511843346091975	27.220280690076	21.77206412603317
14-15	24.68630387751706	24.248904421146058	26.65973262328729	24.405059078049593
16-17	22.63687801630887	26.48277583624563	27.595828479502966	23.28451766794253
18-19	22.282687080490376	24.50130360015532	31.14217562545071	22.073833693903588
20-21	23.82093526377101	23.291451711321905	31.160481499972263	21.72713152493482
22-23	23.924113829256115	23.391024574249737	30.78687524269152	21.89798635380263
24-25	22.651023464802797	24.307982470738338	31.00266267265768	22.038331391801186
26-27	22.61746269484662	23.94047817163144	31.14078881677484	22.3012703167471
28-29	22.274366228435124	23.921894935374716	31.555999334331837	22.247739501858323
30-31	22.77112109613358	23.72524546513563	31.364897098796252	22.138736339934542
32-33	22.225273201309147	24.005935541132743	31.29250568591557	22.47628557164254
34-35	22.493481999223384	23.783768791257557	31.13440949686581	22.588339712653244
36-37	22.543129749819716	23.876684972541188	30.820436012647694	22.759749264991402
38-39	22.68264270261275	23.67837133189105	30.796582903422642	22.842403062073558
40-41	22.311965894938883	23.955088715873444	30.810217095245097	22.92272829394258
42-43	22.758917179785875	23.862816885782436	30.303433738281466	23.07483219615022
44-45	22.67792755311477	24.10134797803295	30.05020247406668	23.170521994785602
46-47	22.67342359209917	24.04034184051326	29.654192982894106	23.632041584493464
48-49	22.922225982369227	24.12442511359034	29.405613221416566	23.54773568262387
50-51	22.79608365229933	24.45692572252732	28.984023964053918	23.76296666111943
52-53	22.50318965995451	24.68630387751706	28.704443334997503	24.106063127530923
54-55	22.568647029455814	24.407000610195816	28.683918566594553	24.340433793753814
56-57	22.793032673212405	24.35679813612914	28.289232817440507	24.560936373217952
58-59	22.706218450102625	24.330171409552335	28.671992011982027	24.29161812836301
60-61	22.842125700338382	24.54873245687025	28.614855494535973	23.994286348255393
62-63	22.452432462417484	24.580351694680203	28.820103178565486	24.147112664336827
64-65	22.216952349253898	24.375381372385867	28.981527708437344	24.42613856992289
66-67	22.620302462081284	24.427751950026074	28.981881123303783	23.97006446458886
68-69	22.600266267265766	24.141010706162977	29.253064847173682	24.00565817939757
70-71	22.370056027070508	24.162090198036278	29.561491096688304	23.906362678204914
72-73	22.555333666167414	24.253064847173682	29.833028235424642	23.35857325123426
74-75	22.44838486751723	24.306224345079666	29.85615204642656	23.389238740976545
76-77	22.377127637438097	24.257078252666915	29.830621332556497	23.535172777338495
78-79	22.370395435722624	24.26394925375618	29.943501257838957	23.422154052682234
80-81	22.096854717923115	24.585898929383703	29.866589005380817	23.450657347312365
82-83	22.356465302046928	24.44583125312032	30.025517279636098	23.17218616519665
84-85	22.691792206494867	24.24405553073587	29.465809416250206	23.598342846519063
86-87	22.132930966929816	24.507948681610912	29.601996270885955	23.757124080573316
88-89	22.52219448876239	24.22326238138387	29.28171574377248	23.972827386081264
90-91	22.56404559907802	24.20361293547536	29.187020091920186	24.04532137352643
92-93	22.42903469414953	24.420283416996135	29.203500510861357	23.947181377992983
94-95	21.8172740888667	24.518500027736174	29.116880235202753	24.547345648194373
96-97	22.307649636656127	24.686858600987406	28.41210406612304	24.593387696233428
98-99	22.275475675375827	24.838298108392966	28.28701392355911	24.599212292672103
100-101	22.37477117656848	25.103733288955453	27.768624840517003	24.75287069395906
102-103	22.512065235480115	25.034670216896878	27.801908248738005	24.651356298885005
104-105	22.54159062290244	25.225080296667755	27.673088550998788	24.560240529431017
106-107	22.434194103125954	25.31883615787868	27.539178431753253	24.707791307242115
108-109	22.488620887640106	25.453430930660225	27.177009339054557	24.880938842645115
110-111	22.175132232574008	26.090381345707396	27.055602861271595	24.678883560446998
112-113	22.2957230820436	26.002385310922506	26.8228213235702	24.879070283463694
114-115	22.292733934470334	26.013551931968525	27.03452331093169	24.65919082262945
116-117	22.42253958656345	25.955305806029305	26.886966458552337	24.73518814885491
118-119	22.087147057191988	25.845675930548623	27.14705719198979	24.920119820269594
120-121	22.20832316329021	25.808241063305744	27.370082321876314	24.613353451527725
122-123	22.081322460753313	26.00987407777223	27.353136961224827	24.555666500249625
124-125	22.047484329061962	25.930825983247352	27.376157985244355	24.64553170244633
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	35.0
1	45.0
2	107.5
3	154.5
4	127.0
5	119.5
6	171.0
7	293.0
8	445.5
9	557.5
10	556.0
11	502.0
12	489.0
13	512.5
14	522.5
15	524.5
16	562.0
17	585.5
18	577.0
19	565.0
20	577.5
21	586.0
22	590.0
23	606.0
24	629.0
25	638.5
26	674.5
27	759.0
28	834.0
29	1043.5
30	1353.5
31	1572.5
32	1864.0
33	2155.5
34	2461.5
35	2844.5
36	3219.0
37	3623.0
38	3897.0
39	3984.5
40	4011.5
41	4015.5
42	4053.0
43	4501.0
44	5034.0
45	5477.0
46	6143.0
47	6648.5
48	7084.5
49	7513.5
50	7884.5
51	7746.5
52	7259.0
53	7008.0
54	6808.5
55	6532.0
56	6090.5
57	5497.5
58	5004.0
59	4503.5
60	3940.5
61	3273.5
62	2611.0
63	2085.5
64	1575.5
65	1282.0
66	972.0
67	730.5
68	646.5
69	543.5
70	434.0
71	370.0
72	344.0
73	321.0
74	242.0
75	194.5
76	168.5
77	116.0
78	78.5
79	50.5
80	37.0
81	27.0
82	21.5
83	13.5
84	7.0
85	5.0
86	4.5
87	3.5
88	1.5
89	1.0
90	1.5
91	2.0
92	1.5
93	0.5
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.027736173517501527
2	0.0
3	0.0
4	0.0
5	5.547234703500305E-4
6	0.0027736173517501524
7	0.006101958173850335
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0019415321462251068
42-43	0.0
44-45	0.0
46-47	0.0058245964386753205
48-49	0.009430298995950518
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.008875575525600489
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0635158373550785
76-77	0.0291229821933766
78-79	2.7736173517501527E-4
80-81	0.0
82-83	0.0
84-85	0.04520996283352749
86-87	0.1855550008320852
88-89	0.2124590891440617
90-91	0.1234259721528818
92-93	0.10095967160370556
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	5.547234703500305E-4
106-107	0.0027736173517501524
108-109	0.0030509790869251677
110-111	2.7736173517501527E-4
112-113	0.0
114-115	2.7736173517501527E-4
116-117	2.7736173517501527E-4
118-119	0.0
120-121	0.001109446940700061
122-123	0.0
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	180270.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.3062423637909
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.51934765341575	51.77896685174891
2	12.62906364459443	16.242552551140594
3	2.9980851578071595	5.783867723556944
4	1.1447299432294178	2.9445312468159814
5	0.615911204308931	1.9803467589432224
6	0.40088658869014426	1.5467706079621064
7	0.2745385799723345	1.2358181123338354
8	0.2277851195287654	1.1718404082625495
9	0.17805001393195488	1.0304754613906173
>10	0.9479417867987658	10.64952041652629
>50	0.04774523079178194	2.03088635751279
>100	0.012931000006107609	1.479508707063648
>500	9.946923081621238E-4	0.4356011368397511
>1k	0.0019893846163242476	1.6893136599027643
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1324	0.7344538747434404	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1317	0.7305708104509901	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	681	0.37776668330837077	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	343	0.19027015033006048	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	308	0.1708548288678094	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	209	0.11593720530315638	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	206	0.11427303489210629	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	185	0.10262384201475563	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	5.547234703500305E-4	0.0
4	0.0	0.0	0.0	5.547234703500305E-4	0.0
5	0.0	0.0	0.0	5.547234703500305E-4	0.0
6	0.0	0.0	0.0	5.547234703500305E-4	0.0
7	0.0	0.0	0.0	5.547234703500305E-4	0.0
8	0.0	0.0	0.0	5.547234703500305E-4	0.0
9	0.0	0.0	0.0	5.547234703500305E-4	0.0
10-11	0.0	0.0	0.0	8.320852055250458E-4	0.0
12-13	0.0	0.0	0.0	0.0019415321462251068	0.0
14-15	0.0	0.0	0.0	0.002218893881400122	0.0
16-17	0.0	0.0	0.0	0.0027736173517501524	0.0
18-19	0.0	0.0	0.0	0.0027736173517501524	0.0
20-21	0.0	0.0	0.0	0.0030509790869251677	0.0
22-23	0.0	0.0	0.0	0.006101958173850336	0.0
24-25	0.0	0.0	0.0	0.017751151051200977	0.0
26-27	0.0	0.0	0.0	0.02995506739890165	0.0
28-29	0.0	0.0	0.0	0.06018749653297831	0.0
30-31	0.0	0.0	0.0	0.1128862262162312	0.0
32-33	0.0	0.0	0.0	0.1783435957175348	0.0
34-35	0.0	0.0	0.0	0.24879347645198868	0.0
36-37	0.0	0.0	0.0	0.3419870194707938	0.0
38-39	0.0	0.0	0.0	0.45931103344982527	0.0
40-41	0.0	0.0	0.0	0.599656071448383	0.0
42-43	0.0	0.0	0.0	0.7358406834193155	0.0
44-45	0.0	0.0	0.0	0.8592666555721973	0.0
46-47	0.0	0.0	0.0	0.9810284573140289	0.0
48-49	0.0	0.0	0.0	1.1061185998779608	0.0
50-51	0.0	0.0	0.0	1.2145670383313918	0.0
52-53	0.0	0.0	0.0	1.3346646696621733	0.0
54-55	0.0	0.0	0.0	1.4411715759693793	0.0
56-57	0.0	0.0	0.0	1.5601597603594608	0.0
58-59	0.0	0.0	0.0	1.691074499362068	0.0
60-61	0.0	0.0	0.0	1.8103400454873246	0.0
62-63	0.0	0.0	0.0	1.9215621012925057	0.0
64-65	0.0	0.0	0.0	2.0289010928052367	0.0
66-67	0.0	0.0	0.0	2.147889277195318	0.0
68-69	0.0	0.0	0.0	2.2788040161979253	0.0
70-71	0.0	0.0	0.0	2.429411438397959	0.0
72-73	0.0	0.0	0.0	2.566705497309591	0.0
74-75	0.0	0.0	0.0	2.690686192932823	0.0
76-77	0.0	0.0	0.0	2.8227103788761303	0.0
78-79	0.0	0.0	0.0	2.9741498862816886	0.0
80-81	0.0	0.0	0.0	3.1344649692128472	0.0
82-83	0.0	0.0	0.0	3.2969989460254063	0.0
84-85	0.0	0.0	0.0	3.4606423697786655	0.0
86-87	0.0	0.0	0.0	3.6179064736228987	0.0
88-89	0.0	0.0	0.0	3.7793310034947583	0.0
90-91	0.0	0.0	0.0	3.957674599212293	0.0
92-93	0.0	0.0	0.0	4.128252066344928	0.0
94-95	0.0	0.0	0.0	4.304376768181061	0.0
96-97	0.0	0.0	0.0	4.495756365451822	0.0
98-99	0.0	0.0	0.0	4.686026515781883	0.0
100-101	0.0	0.0	0.0	4.90209130748322	0.0
102-103	0.0	0.0	0.0	5.117324013979031	0.0
104-105	0.0	0.0	0.0	5.329505741387917	0.0
106-107	0.0	0.0	0.0	5.555000832085206	0.0
108-109	0.0	0.0	0.0	5.7824374549287185	0.0
110-111	0.0	0.0	0.0	6.001830587452155	2.7736173517501527E-4
112-113	0.0	0.0	0.0	6.264492150662894	5.547234703500305E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGTA	15	0.004231049	59.511166	68-69
GGTATCA	1465	0.0	41.846424	1
GCGCAGA	75	8.1454804E-5	39.679615	1
CTGACCG	70	0.0027859015	33.992233	9
TATGACT	75	0.0039061862	31.726082	4
TAGTAGT	75	0.0039061862	31.726082	4
GTATCAA	1995	0.0	30.71228	2
CGCATAA	70	3.0921492E-6	29.743204	106-107
ATCAACG	2200	0.0	28.120848	4
CAACGCA	2255	0.0	27.69877	6
TATCAAC	2260	0.0	27.63749	3
TCAACGC	2265	0.0	27.576479	5
ATGGGGG	490	0.0	26.708185	5
AACGCAG	2340	0.0	26.69262	7
ACGCAGA	2395	0.0	26.079638	8
CATGGGG	720	0.0	25.612204	4
CGCAGAG	2490	0.0	25.084631	9
GAGTACT	1310	0.0	24.748161	12-13
CAGAGTA	2615	0.0	23.203112	10-11
GCAGAGT	2640	0.0	22.53273	9
>>END_MODULE