FastQCFastQC Report
Sun 1 Jan 2017
ERR1142495_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142495_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419437
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9780.23316970129006265No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5060.12063790271244548No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG700.002781430734.0137984
TCGAGAT800.005347735229.7620747
GTATCAA19050.028.7534431
AATTGCC1701.3916175E-524.5099417
GGTATCA14600.024.0600281
ATCAACG21350.023.9769383
CAACGCA21400.023.6427675
AACGCAG21050.023.464726
TATCAAC22350.023.43682
CATGGGT4850.023.3187394
TAGTACT1300.002045162522.89394
TCAACGC22650.022.337984
GTACATG31250.021.3385541
CATGGGG14000.020.833454
ACATGGG30450.020.7210483
ACGCAGA23100.020.6144227
TACATGG32100.020.3976822
GTACTGT2357.3652755E-620.2586966
CGCAGAG23600.020.1776758
CTGTTAC1856.915115E-419.3097461
Produced by FastQC (version 0.11.5)