FastQCFastQC Report
Sun 1 Jan 2017
ERR1142495_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142495_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419437
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17720.42247107432105424No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17390.41460338501372074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9040.21552700405543623No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4490.10704825754523324No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC4340.10347203513280898No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16650.047.8713951
TATCACG505.3398026E-447.5799182
GTATCAA24350.034.687651
AACCGTC800.005369431329.7374487
CAACGCA29900.028.2456075
ATCAACG30050.027.7087753
TATCAAC30650.027.5544412
TCAACGC30800.027.4202464
AACGCAG31400.027.0857036
GTAACAC1107.7620905E-427.0340463
ACGCAGA34250.024.4845587
CGCAGAG34650.024.02748
GTACATG29050.023.9566061
ACATGGG28600.023.706783
TACATGG30150.022.8825472
GAGTACT17350.022.79313912-13
CAGAGTA36000.022.63080610-11
GCAGAGT37000.022.5013359
CATGGGG13500.022.4682964
AGAGTAC35350.020.27120610-11
Produced by FastQC (version 0.11.5)