ERR1142484_2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1142484_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	68928
Sequences flagged as poor quality	0
Sequence length	125
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	342	0.49616991643454034	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	264	0.383008356545961	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	206	0.29886258124419685	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	170	0.24663416898792942	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	149	0.21616759517177347	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	147	0.21326601671309192	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	138	0.20020891364902507	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	135	0.19585654596100277	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	127	0.1842502321262767	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	124	0.17989786443825442	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	115	0.16684076137418757	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	115	0.16684076137418757	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	115	0.16684076137418757	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	114	0.1653899721448468	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	109	0.15813602599814297	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	101	0.1465297121634169	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	99	0.14362813370473537	No Hit
GGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGA	95	0.13782497678737235	No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	93	0.1349233983286908	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	91	0.13202181987000927	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	88	0.127669452181987	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	84	0.12186629526462396	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	83	0.1204155060352832	No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT	82	0.11896471680594242	No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA	77	0.11171077065923862	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	76	0.11025998142989786	No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC	76	0.11025998142989786	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	74	0.10735840297121634	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	74	0.10735840297121634	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	73	0.10590761374187557	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	72	0.10445682451253482	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	71	0.10300603528319405	No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA	71	0.10300603528319405	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	69	0.10010445682451254	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAG	15	2.6698984E-4	119.23306	1
GTGAAAA	25	0.0020327556	71.53984	1
AGTACTC	25	0.002038641	71.48778	3
GATTTTT	30	0.00418706	59.61653	1
CAGTTCT	30	0.004199162	59.573143	4
AACAGTT	30	0.004199162	59.573143	2
TTGCGAA	15	0.0042324276	59.486553	26-27
TCTCTGC	50	5.265461E-4	47.658516	8
TATTCTC	55	8.421306E-4	43.325924	5
TTCTCTG	55	8.421306E-4	43.325924	7
ATGGGAC	45	5.1048155E-6	39.686546	18-19
GGGACTG	40	1.1884808E-4	37.206135	20-21
TTCATAG	65	0.0019146708	36.660397	7
GCTGCTA	35	0.0027835863	33.992317	26-27
GAGTACT	65	1.7049697E-6	32.077847	12-13
TCATAGC	75	0.0038618671	31.77234	8
GTCTTCT	80	0.00527686	29.808268	1
CATAGCT	80	0.0052957027	29.786572	9
GACTGCT	50	4.4244298E-4	29.743277	22-23
AGGGCTA	105	5.8069447E-4	28.368164	5

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content