FastQCFastQC Report
Sun 1 Jan 2017
ERR1142483_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142483_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818369
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19370.23669029496474087No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17670.2159172695935452No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20850.041.4442331
GTATCAA28650.030.5771
GTTTACG900.00945437226.4861971
TATACGT900.00947420626.4748152
TACGAGT1159.978554E-425.8992754
ATCAACG35050.024.30324
TCAACGC35750.024.1605825
CAACGCA35700.023.5096686
TATCAAC36800.023.3093472
AACGCAG37100.022.4786157
GTACATG56900.020.63271
CATGGGT11150.020.3013154
ACGCAGA41650.020.0266578
TACATGG58350.020.0092432
ACATGGG58500.019.652463
ATAAGAC2158.4830994E-519.394343
CCGTCGA1856.876656E-419.328959
CGCAGAG43700.019.0930549
GTCTAGT1600.006769080618.6231061
CAGAGTA44850.018.47060610-11
Produced by FastQC (version 0.11.5)