FastQCFastQC Report
Sun 1 Jan 2017
ERR1142483_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142483_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818369
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33160.4051961890052042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31820.3888221572420265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21310.26039598274128173No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28050.049.0052381
GTATCAA43550.041.538311
ATCAACG55450.033.470113
TATCAAC56700.032.9420552
TCAACGC56950.032.7974434
CAACGCA57050.032.7399565
AACGCAG58850.031.7366266
ACGCAGA65200.028.8281697
CGCAGAG68400.027.561398
GCAGAGT73500.025.6489689
GTAAGTA3301.8189894E-1225.2358652
GAGTACT31500.023.03282512-13
CAGAGTA71900.022.62176110-11
AGAGTAC69250.021.81282410-11
GTACTTT35100.020.75519814-15
AGTACTT33250.020.2108612-13
AGTACAC5550.019.2922825
GTACATG58700.019.1596281
CATGGGG29200.018.7417034
GAGTACA49750.018.34903512-13
Produced by FastQC (version 0.11.5)