FastQCFastQC Report
Sun 1 Jan 2017
ERR1142473_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142473_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974031
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23210.2382881037667179No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21680.22258018481957964No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24200.034.7009121
GTATCAA34150.028.6015951
CATGGGG32750.025.0895544
GTACATG67100.024.4976081
CATGGGT15500.024.2010384
TACATGG68800.023.8861312
CAACGCA40500.023.8168965
TCAACGC40450.023.6991374
ATCAACG40550.023.6406923
ACATGGG67150.023.4976833
AACGCAG41900.023.4341356
TATCAAC42750.022.8419342
ATGGGGG15900.021.3453315
AACGCAC1400.00314705221.2596057
ACGCAGA46650.021.054537
CGCAGAG48200.020.2591848
ATGGGTG8550.020.1956145
GCAGAGT52100.018.6351039
AGAGTAC49100.017.46879210-11
CAGAGTA51750.017.207310-11
Produced by FastQC (version 0.11.5)