FastQCFastQC Report
Sun 1 Jan 2017
ERR1142473_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142473_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974031
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40460.4153871899354332No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34600.35522483370652475No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24100.2474253899516545No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26300.053.1489071
GTATCAA42350.043.6806561
ATCAACG56200.033.4399833
CAACGCA56900.033.235935
TCAACGC57650.033.0098574
TATCAAC58050.032.3742832
AACGCAG59350.032.1645366
ACGCAGA66850.028.464077
CGCAGAG69750.027.2806178
GCAGAGT74950.025.38799
AGAGTAC71650.022.9055810-11
CAGAGTA74950.022.33341810-11
GTACATG62400.022.1149311
TACATGG63800.022.0924032
GAGTACT33900.021.83826312-13
CATGGGT15550.021.7990444
AGTACTT35950.021.50269512-13
ACATGGG64250.021.2897223
CATGGGG32150.021.08714
GTACTTT38400.019.2790914-15
Produced by FastQC (version 0.11.5)