##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142471_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	853204
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.322520757052242	33.0	33.0	33.0	27.0	33.0
2	31.4293896887497	33.0	33.0	33.0	27.0	33.0
3	31.525329229586358	33.0	33.0	33.0	27.0	33.0
4	31.517135409585514	33.0	33.0	33.0	27.0	33.0
5	31.503032100177684	33.0	33.0	33.0	27.0	33.0
6	34.881760985649386	37.0	37.0	37.0	27.0	37.0
7	34.855463640583025	37.0	37.0	37.0	27.0	37.0
8	34.89733170496153	37.0	37.0	37.0	33.0	37.0
9	34.87618201508666	37.0	37.0	37.0	27.0	37.0
10-11	34.93756241180304	37.0	37.0	37.0	33.0	37.0
12-13	34.877378094804996	37.0	37.0	37.0	30.0	37.0
14-15	34.86805383003362	37.0	37.0	37.0	27.0	37.0
16-17	34.824409519880355	37.0	37.0	37.0	27.0	37.0
18-19	34.87429852649542	37.0	37.0	37.0	27.0	37.0
20-21	34.85686189938163	37.0	37.0	37.0	27.0	37.0
22-23	34.92187741735857	37.0	37.0	37.0	27.0	37.0
24-25	34.978847966019856	37.0	37.0	37.0	27.0	37.0
26-27	35.014279117303715	37.0	37.0	37.0	30.0	37.0
28-29	35.03548740981055	37.0	37.0	37.0	33.0	37.0
30-31	34.976047932264734	37.0	37.0	37.0	27.0	37.0
32-33	34.991578215760825	37.0	37.0	37.0	27.0	37.0
34-35	35.04682584704244	37.0	37.0	37.0	33.0	37.0
36-37	35.03172394878599	37.0	37.0	37.0	33.0	37.0
38-39	35.00534280195592	37.0	37.0	37.0	30.0	37.0
40-41	34.968788824243674	37.0	37.0	37.0	27.0	37.0
42-43	34.9706060918608	37.0	37.0	37.0	27.0	37.0
44-45	34.964497939531455	37.0	37.0	37.0	27.0	37.0
46-47	34.953323589669054	37.0	37.0	37.0	27.0	37.0
48-49	34.95350291372286	37.0	37.0	37.0	27.0	37.0
50-51	34.881170857145534	37.0	37.0	37.0	27.0	37.0
52-53	34.90714237157819	37.0	37.0	37.0	27.0	37.0
54-55	34.87631562908753	37.0	37.0	37.0	27.0	37.0
56-57	34.84583288404649	37.0	37.0	37.0	27.0	37.0
58-59	34.82050599856541	37.0	37.0	37.0	27.0	37.0
60-61	34.80492531680583	37.0	37.0	37.0	27.0	37.0
62-63	34.780911130280685	37.0	37.0	37.0	27.0	37.0
64-65	34.7419327616842	37.0	37.0	37.0	27.0	37.0
66-67	34.70119572810254	37.0	37.0	37.0	27.0	37.0
68-69	34.664479421099756	37.0	37.0	37.0	27.0	37.0
70-71	34.6194995569641	37.0	37.0	37.0	27.0	37.0
72-73	34.55813849911627	37.0	37.0	37.0	27.0	37.0
74-75	34.478127153646724	37.0	37.0	37.0	27.0	37.0
76-77	34.43325746245915	37.0	37.0	37.0	27.0	37.0
78-79	34.38742668810741	37.0	37.0	37.0	27.0	37.0
80-81	34.33129357105686	37.0	37.0	37.0	27.0	37.0
82-83	34.27618658609195	37.0	37.0	37.0	27.0	37.0
84-85	34.22803163135663	37.0	37.0	37.0	27.0	37.0
86-87	34.151970689307596	37.0	37.0	37.0	27.0	37.0
88-89	34.10088618900052	37.0	37.0	37.0	27.0	37.0
90-91	34.00942095911412	37.0	37.0	37.0	27.0	37.0
92-93	33.945489589828455	37.0	37.0	37.0	27.0	37.0
94-95	33.88519744398761	37.0	37.0	37.0	24.5	37.0
96-97	33.792966863727784	37.0	33.0	37.0	22.0	37.0
98-99	33.69541750859115	37.0	33.0	37.0	22.0	37.0
100-101	33.59881458596068	37.0	33.0	37.0	22.0	37.0
102-103	33.49213787089606	37.0	33.0	37.0	22.0	37.0
104-105	33.36037864332563	37.0	33.0	37.0	22.0	37.0
106-107	33.26233819813315	37.0	33.0	37.0	22.0	37.0
108-109	33.161507681633	37.0	33.0	37.0	14.0	37.0
110-111	33.08618396069404	37.0	33.0	37.0	14.0	37.0
112-113	32.94239361278194	37.0	33.0	37.0	14.0	37.0
114-115	32.837576359229445	37.0	33.0	37.0	14.0	37.0
116-117	32.63613450007267	37.0	33.0	37.0	14.0	37.0
118-119	32.567386580466106	37.0	33.0	37.0	14.0	37.0
120-121	32.470618984439824	37.0	33.0	37.0	14.0	37.0
122-123	32.38368022184612	37.0	33.0	37.0	14.0	37.0
124-125	30.75442039652885	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1661.0
1	91.0
2	59.0
3	32.0
4	36.0
5	38.0
6	38.0
7	44.0
8	33.0
9	36.0
10	21.0
11	38.0
12	36.0
13	28.0
14	47.0
15	227.0
16	336.0
17	690.0
18	1426.0
19	2691.0
20	3961.0
21	5165.0
22	5954.0
23	7038.0
24	7906.0
25	9190.0
26	10796.0
27	13054.0
28	15823.0
29	19283.0
30	22899.0
31	28504.0
32	36257.0
33	49390.0
34	74034.0
35	138132.0
36	398210.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.893651195217956	19.07983220469615	12.122681194806113	24.903835405279786
2	17.778964479710925	22.210119953608842	35.58945236319666	24.42146320348357
3	20.38445117971046	25.970095889331503	27.809435096224526	25.83601783473351
4	15.247454190721365	18.35158334695567	33.449329016522036	32.95163344580093
5	16.310883803214516	36.15293409116573	31.087439304400196	16.448742801219556
6	30.900892004871466	34.586701274762895	18.066977951445217	16.445428768920426
7	27.196884382178066	31.788141749796043	21.43209801077253	19.582875857253356
8	25.774135004631177	33.242081160755845	20.417017335170133	20.56676649944285
9	26.500668897107403	17.659001192024018	19.812050492914864	36.02827941795372
10-11	25.62184960878724	26.254161337646703	25.647652253235027	22.476336800331026
12-13	26.38492904621771	24.959620874258786	25.183697828317424	23.471752251206084
14-15	24.802996755240674	25.403889005230674	23.241323974747818	26.551790264780834
16-17	24.661796234509897	27.289571575242178	23.88495368110822	24.163678509139704
18-19	24.67977077896232	26.47336013889191	25.110133453584034	23.736735628561735
20-21	26.095748920975808	25.5028293889625	24.59322757269819	23.808194117363502
22-23	25.890333654682685	25.330421594676572	24.46177902137365	24.317465729267088
24-25	25.050971559956015	25.87315795629464	24.599647709967858	24.476222773781487
26-27	24.73031630856763	25.94247492425111	24.642679762188152	24.68452900499311
28-29	24.76237660421137	25.697883736880243	24.98523393849208	24.554505720416305
30-31	24.840625449971938	25.72602329128208	24.89992624133483	24.533425017411158
32-33	24.604450744552935	25.871348493557083	24.651701558218996	24.87249920367099
34-35	24.918157634477982	25.812121308279078	24.791405537955992	24.478315519286944
36-37	24.8726994072332	25.898508592385998	24.522868857324024	24.705923143056776
38-39	24.760826912194787	25.8245823101526	24.58524821644834	24.829342561204275
40-41	24.682771395594294	25.929354047808783	24.833569579904232	24.55430497669269
42-43	24.762174508586046	25.786341656613427	24.823992389114178	24.627491445686353
44-45	24.66557645423194	25.970246954858034	24.6487120470041	24.715464543905927
46-47	24.735989770999183	25.774637624953872	24.70790232620453	24.781470277842413
48-49	24.74876084335509	25.985508506642912	24.71485883367686	24.55087181632514
50-51	24.371591185529997	26.276531608766522	24.75441258216117	24.59746462354231
52-53	24.502509633679274	25.90416736688835	24.852072048991623	24.741250950440758
54-55	24.48739630109731	25.78554967029067	24.926107737562887	24.800946291049137
56-57	24.669980668323667	25.753435926363355	24.616980145369503	24.959603259943474
58-59	24.628250385439777	25.783159572067838	24.796468995600346	24.792121046892042
60-61	24.666591071062413	25.85933694821329	24.903835928553928	24.570236052170365
62-63	24.308106482036386	26.035255076980206	24.768683357657714	24.887955083325693
64-65	24.374601589236665	25.712556732223902	24.992435703479575	24.920405975059854
66-67	24.661756264757496	25.688781100533987	24.823497548030346	24.82596508667817
68-69	24.707034635277584	25.69816679622067	24.67313320325054	24.921665365251208
70-71	24.508268284170935	25.739668610601598	24.79874221965799	24.953320885569486
72-73	24.6772312708367	25.81826726784062	24.786441275389564	24.718060185933115
74-75	24.69425527789366	25.72650727711806	24.911951865844845	24.66728557914343
76-77	24.762213708831457	25.589477872138115	24.834791050714227	24.813517368316198
78-79	24.67799924439036	25.645661384645845	24.89516356950933	24.78117580145446
80-81	24.547209335805174	25.75032244606557	24.930090167378154	24.7723780507511
82-83	24.60674104488311	25.761840073516453	24.96051546125227	24.67090342034817
84-85	24.54831922280764	25.81430042875081	24.768305931943964	24.869074416497593
86-87	24.411014758413234	25.896197593532623	24.87632778717804	24.816459860876105
88-89	24.56892500305477	25.52054450094463	24.8922018779784	25.018328618022203
90-91	24.828108800300754	25.552093092925514	24.819943784749043	24.79985432202469
92-93	24.59195416140669	25.860986265222596	24.8493674224779	24.69769215089281
94-95	24.17277106494657	25.929991935204388	25.026711331419634	24.87052566842941
96-97	24.383317533763503	25.702728450620178	24.988634881926114	24.925319133690206
98-99	24.242787332017667	25.93112724368011	24.84288365755098	24.983201766751247
100-101	24.417459449586758	26.101140679498013	24.63287480245273	24.8485250684625
102-103	24.458354525389943	25.951469505168905	24.949050967826054	24.641125001615098
104-105	24.335731448671932	26.121719477477512	24.92148712518068	24.62106194866988
106-107	24.304276532165613	26.088984006465072	24.973512469694974	24.63322699167434
108-109	24.31956238683355	26.048413951797478	24.83850563607842	24.793518025290552
110-111	24.21896381808849	26.408226119621254	24.700148119957195	24.67266194233306
112-113	24.292562947406253	26.377742579420943	24.650223670092338	24.679470803080463
114-115	24.165751566436278	26.425354246554427	24.82761860579831	24.581275581210985
116-117	24.18513800723878	26.557151188091428	24.697948581519675	24.55976222315012
118-119	24.080221405724345	26.34401624957353	24.8664513132312	24.709311031470925
120-121	24.060247069593952	26.30720978835777	24.950364696894336	24.682178445153944
122-123	24.12589773656764	26.507883288227536	24.894526412582728	24.4716925626221
124-125	24.11523924109426	26.412848621076186	24.700431172421407	24.771480965408145
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2641.0
1	1537.0
2	364.5
3	270.0
4	222.5
5	184.0
6	156.5
7	153.0
8	150.0
9	140.0
10	140.0
11	131.0
12	133.5
13	145.0
14	170.5
15	194.5
16	213.5
17	235.0
18	269.5
19	330.5
20	398.0
21	490.5
22	622.5
23	815.0
24	1032.5
25	1304.5
26	1634.5
27	2033.0
28	2650.0
29	3547.5
30	4655.5
31	5668.0
32	6821.5
33	8121.0
34	9254.5
35	10576.0
36	12112.0
37	13962.0
38	15611.5
39	16800.0
40	18119.5
41	19450.5
42	20829.5
43	22807.0
44	25255.5
45	27524.5
46	29920.0
47	32333.0
48	34676.0
49	36626.5
50	37457.5
51	36808.0
52	35925.0
53	36160.0
54	36047.5
55	35187.5
56	33719.0
57	31119.0
58	28935.0
59	26415.0
60	22767.0
61	19258.0
62	15880.5
63	12525.5
64	9778.5
65	7889.5
66	6267.5
67	4953.0
68	4135.5
69	3483.5
70	2959.0
71	2542.0
72	2125.0
73	1951.5
74	1688.0
75	1445.5
76	1143.0
77	808.0
78	587.0
79	369.5
80	263.5
81	186.0
82	115.0
83	79.0
84	47.5
85	24.0
86	12.0
87	6.0
88	4.5
89	3.5
90	2.0
91	1.5
92	2.0
93	1.5
94	1.5
95	2.0
96	3.5
97	2.5
98	0.0
99	0.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.39369248151672986
2	0.35653841285319804
3	0.3461071443640677
4	0.3381371864173164
5	0.3586481076038087
6	0.29723254930825455
7	0.29559167561333516
8	0.286918486083047
9	0.29887342300317393
10-11	0.29488844402979825
12-13	0.3312806784778318
14-15	0.3771665393036132
16-17	0.340246881167927
18-19	0.3233107205310805
20-21	0.31258643888214305
22-23	0.3075466125334621
24-25	0.2911964782162296
26-27	0.29670512562060186
28-29	0.2854534202840118
30-31	0.28791473082639085
32-33	0.2842227650128223
34-35	0.27314686757211637
36-37	0.2770146412815693
38-39	0.26998232544620043
40-41	0.2810582228869063
42-43	0.2716231991411198
44-45	0.2695135043905092
46-47	0.26840005438324244
48-49	0.2604300964364912
50-51	0.2678140280636284
52-53	0.26757961753578274
54-55	0.269923722814239
56-57	0.26593874384086336
58-59	0.26113332802002803
60-61	0.25603489903938564
62-63	0.2585548122137261
64-65	0.253925204288775
66-67	0.2524015358577784
68-69	0.25873062010961034
70-71	0.2556246806156558
72-73	0.245134809494564
74-75	0.2635946385624071
76-77	0.27982756761571675
78-79	0.2620123674994492
80-81	0.26798983595951265
82-83	0.2623053806592562
84-85	0.26295000961083165
86-87	0.25363219112896795
88-89	0.24331812790376042
90-91	0.23575838838073895
92-93	0.239508956826269
94-95	0.23130458835167203
96-97	0.22444808041218747
98-99	0.22550292778749279
100-101	0.21395820929109569
102-103	0.21835340668820116
104-105	0.2239206567245348
106-107	0.21800179089643273
108-109	0.2178259830005485
110-111	0.21882222774389243
112-113	0.21548187772209224
114-115	0.20499200660100045
116-117	0.21302056717971318
118-119	0.20340973553804245
120-121	0.204347377649425
122-123	0.19778388286974743
124-125	0.19790108813367027
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	853204.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.71042595894505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.34713158138841	70.49824196225882
2	9.07314116121337	14.645941758143366
3	1.8953581390523722	4.589254882430111
4	0.7082093410795488	2.286395103465367
5	0.3361447099147936	1.3565191360534505
6	0.20410401394924307	0.9883993143464321
7	0.109795133611241	0.6203128401387782
8	0.07616052716532805	0.49175588710171464
9	0.05117718745015514	0.37174793386345184
>10	0.18332981102802304	2.4263791068327705
>50	0.010299122192724733	0.5493342016026839
>100	0.004794149753573424	0.6974913337281079
>500	0.0	0.0
>1k	3.551222012646945E-4	0.4782265400349403
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	1879	0.2202286909109662	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1458	0.1708852747994618	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.172052639228133E-4
7	0.0	0.0	0.0	0.0	1.172052639228133E-4
8	0.0	0.0	0.0	0.0	1.172052639228133E-4
9	0.0	0.0	0.0	0.0	1.172052639228133E-4
10-11	0.0	0.0	0.0	5.860263196140665E-5	1.172052639228133E-4
12-13	0.0	0.0	0.0	1.172052639228133E-4	1.172052639228133E-4
14-15	0.0	0.0	0.0	1.172052639228133E-4	1.172052639228133E-4
16-17	0.0	0.0	0.0	1.7580789588421996E-4	1.172052639228133E-4
18-19	0.0	0.0	0.0	3.5161579176843993E-4	1.172052639228133E-4
20-21	0.0	0.0	0.0	6.446289515754732E-4	1.172052639228133E-4
22-23	0.0	0.0	0.0	0.0016994763268807927	1.172052639228133E-4
24-25	0.0	1.172052639228133E-4	0.0	0.004160786869259872	1.172052639228133E-4
26-27	0.0	1.172052639228133E-4	0.0	0.00802856057871271	1.172052639228133E-4
28-29	0.0	1.172052639228133E-4	0.0	0.01593991589350261	1.172052639228133E-4
30-31	0.0	1.172052639228133E-4	0.0	0.031879831787005215	1.172052639228133E-4
32-33	0.0	1.172052639228133E-4	0.0	0.0571961687943329	1.172052639228133E-4
34-35	0.0	1.172052639228133E-4	0.0	0.08936901374114514	1.172052639228133E-4
36-37	0.0	1.172052639228133E-4	0.0	0.13830221142891969	1.172052639228133E-4
38-39	0.0	1.172052639228133E-4	0.0	0.20587104608042156	1.172052639228133E-4
40-41	0.0	1.172052639228133E-4	0.0	0.2741431123154603	1.172052639228133E-4
42-43	0.0	1.172052639228133E-4	0.0	0.33696513377808823	1.172052639228133E-4
44-45	0.0	1.172052639228133E-4	0.0	0.40312750526251634	1.7580789588421996E-4
46-47	0.0	1.172052639228133E-4	0.0	0.4806001847154959	2.344105278456266E-4
48-49	0.0	1.172052639228133E-4	0.0	0.560299764183009	2.344105278456266E-4
50-51	0.0	1.172052639228133E-4	0.0	0.6416988199774029	2.344105278456266E-4
52-53	0.0	1.172052639228133E-4	0.0	0.7161241625683893	2.930131598070333E-4
54-55	0.0	1.172052639228133E-4	0.0	0.789143041992302	3.5161579176843993E-4
56-57	0.0	1.172052639228133E-4	0.0	0.8694286477794291	3.5161579176843993E-4
58-59	0.0	1.172052639228133E-4	0.0	0.9654197589322131	4.102184237298466E-4
60-61	0.0	1.172052639228133E-4	0.0	1.0605904332375375	4.688210556912532E-4
62-63	0.0	1.172052639228133E-4	0.0	1.1502524601384898	4.688210556912532E-4
64-65	0.0	1.172052639228133E-4	0.0	1.2327649659401503	4.688210556912532E-4
66-67	0.0	1.172052639228133E-4	0.0	1.325825945494864	4.688210556912532E-4
68-69	0.0	2.344105278456266E-4	0.0	1.428849372483017	4.688210556912532E-4
70-71	0.0	2.344105278456266E-4	0.0	1.5404873863694966	4.688210556912532E-4
72-73	0.0	2.344105278456266E-4	0.0	1.6531216449993202	4.688210556912532E-4
74-75	0.0	2.344105278456266E-4	0.0	1.7571999193627783	4.688210556912532E-4
76-77	0.0	2.344105278456266E-4	0.0	1.8663180200749176	4.688210556912532E-4
78-79	0.0	2.344105278456266E-4	0.0	1.990614202465061	4.688210556912532E-4
80-81	0.0	2.344105278456266E-4	0.0	2.1200674164678084	4.688210556912532E-4
82-83	0.0	2.344105278456266E-4	0.0	2.259190064744188	4.688210556912532E-4
84-85	0.0	2.344105278456266E-4	0.0	2.3896981261222408	4.688210556912532E-4
86-87	0.0	2.344105278456266E-4	0.0	2.513584090088654	4.688210556912532E-4
88-89	0.0	2.344105278456266E-4	0.0	2.6605008884159007	4.688210556912532E-4
90-91	0.0	2.344105278456266E-4	0.0	2.82294738421292	4.688210556912532E-4
92-93	0.0	2.344105278456266E-4	0.0	2.98668313791309	4.688210556912532E-4
94-95	0.0	2.344105278456266E-4	0.0	3.1461408994800775	8.204368474596931E-4
96-97	0.0	2.344105278456266E-4	0.0	3.3078255610615983	8.204368474596931E-4
98-99	0.0	2.344105278456266E-4	0.0	3.48662219117585	8.790394794210998E-4
100-101	0.0	2.344105278456266E-4	0.0	3.6808313134959514	9.376421113825064E-4
102-103	0.0	2.344105278456266E-4	0.0	3.874513012128401	9.376421113825064E-4
104-105	0.0	2.344105278456266E-4	0.0	4.079387813465479	9.376421113825064E-4
106-107	0.0	2.344105278456266E-4	0.0	4.279398596349759	0.0010548473753053196
108-109	0.0	2.344105278456266E-4	0.0	4.494177242488314	0.0014064631670737595
110-111	0.0	2.344105278456266E-4	0.0	4.725540433471948	0.001699476326880793
112-113	0.0	2.344105278456266E-4	0.0	4.967276290312751	0.0017580789588421996
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3140	0.0	33.00391	1
GGTATCA	2260	0.0	30.569994	1
CAACGCA	3715	0.0	26.443426	5
ATCAACG	3715	0.0	26.443426	3
AACGCAG	3760	0.0	26.434313	6
TCAACGC	3780	0.0	25.988712	4
TATCAAC	3875	0.0	25.505215	2
ACGCAGA	4180	0.0	22.933361	7
CGCAGAG	4345	0.0	22.199507	8
CATGGGG	2920	0.0	21.205235	4
GCAGAGT	4795	0.0	20.247452	9
GTACATG	5635	0.0	19.870544	1
TACATGG	5765	0.0	19.209064	2
ACATGGG	5695	0.0	18.81791	3
TATTACT	360	4.663343E-8	18.192097	2
AGAGTAC	4270	0.0	18.06397	10-11
GAGTAAA	365	5.4384145E-8	17.949223	1
ATGGGGG	1495	0.0	17.92107	5
CAGAGTA	4575	0.0	17.445566	10-11
GAGTACA	4780	0.0	16.510466	12-13
>>END_MODULE