FastQCFastQC Report
Sun 1 Jan 2017
ERR1142471_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142471_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853204
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31920.37411920244162006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28580.3349726442914004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16340.19151340124987692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33650.040.485561
GTATCAA46200.033.865971
ATCAACG56950.027.470143
TCAACGC58550.027.0242464
CAACGCA57900.027.0194195
TATCAAC58650.026.8767492
AACGCAG60450.026.1733156
AACGCGA1150.001006539725.8610156
TTAGTAC1901.1703978E-625.045823
ACGCGAG1250.001636328223.7921337
ACGCAGA67200.023.4557887
CGCAGAG69400.022.7082398
GAGTACT29750.022.3886612-13
GCAGAGT74000.021.3770129
CAGAGTA73600.020.32156810-11
GTACTTT33350.020.06105214-15
AGAGTAC70800.018.94132410-11
ACATGGG61650.018.911323
GTACATG63250.018.9053761
TACATGG64050.018.8527952
Produced by FastQC (version 0.11.5)