FastQCFastQC Report
Sun 1 Jan 2017
ERR1142470_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142470_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365058
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14280.39117071807767534No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7650.20955574182732606No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5970.16353565734759956No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4790.13121202658207737No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAT850.007147294528.0453571
TACGTCG450.00950263126.4581822-23
TAGACTT1250.001619975123.8287244
CATGGGG17700.023.5594754
GTATAAG1802.1447446E-523.176371
GGTATCA25300.022.6136471
GGGTAAA1602.5781183E-422.3486441
GTGTTAT1350.002528083722.0727351
CCGATCG2004.8263682E-520.8644719
ATGGGGG10450.019.952285
GTATTAT1500.004656023319.8654611
GTATCCA1805.7105924E-419.8654611
ATAAGGT2107.062974E-519.857273
GTATCAA31100.019.1628231
TATGTAA1600.00677656718.616192
TGGGGGG7550.018.1377326
GTAAGTA1650.00809258518.0520635
ATGGGGA4651.4551915E-1017.93565
AGGGCTA2351.6736376E-417.7447955
GTACATG45000.017.0842971
Produced by FastQC (version 0.11.5)