##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142470_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	365058
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.089158435097985	33.0	33.0	33.0	27.0	33.0
2	31.180012491165787	33.0	33.0	33.0	27.0	33.0
3	31.292265886516663	33.0	33.0	33.0	27.0	33.0
4	31.221972946764623	33.0	33.0	33.0	27.0	33.0
5	31.32110513945729	33.0	33.0	33.0	27.0	33.0
6	34.660618312706475	37.0	37.0	37.0	27.0	37.0
7	34.64350870272669	37.0	37.0	37.0	27.0	37.0
8	34.60970311566929	37.0	37.0	37.0	27.0	37.0
9	34.49676489763271	37.0	37.0	37.0	27.0	37.0
10-11	34.62908633696563	37.0	37.0	37.0	27.0	37.0
12-13	34.56160253987038	37.0	37.0	37.0	27.0	37.0
14-15	34.547421231694685	37.0	37.0	37.0	27.0	37.0
16-17	34.53500402675739	37.0	37.0	37.0	27.0	37.0
18-19	34.57982978047324	37.0	37.0	37.0	27.0	37.0
20-21	34.55333125147237	37.0	37.0	37.0	27.0	37.0
22-23	34.65826526195838	37.0	37.0	37.0	27.0	37.0
24-25	34.75001369645372	37.0	37.0	37.0	27.0	37.0
26-27	34.8069430063168	37.0	37.0	37.0	27.0	37.0
28-29	34.85153455067414	37.0	37.0	37.0	27.0	37.0
30-31	34.816508609590805	37.0	37.0	37.0	27.0	37.0
32-33	34.836209588613315	37.0	37.0	37.0	27.0	37.0
34-35	34.9052588903681	37.0	37.0	37.0	27.0	37.0
36-37	34.89716428622302	37.0	37.0	37.0	27.0	37.0
38-39	34.87133414416339	37.0	37.0	37.0	27.0	37.0
40-41	34.84331119986413	37.0	37.0	37.0	27.0	37.0
42-43	34.84914451950101	37.0	37.0	37.0	27.0	37.0
44-45	34.84634222507108	37.0	37.0	37.0	27.0	37.0
46-47	34.838814654109754	37.0	37.0	37.0	27.0	37.0
48-49	34.8467777722992	37.0	37.0	37.0	27.0	37.0
50-51	34.79175363914776	37.0	37.0	37.0	27.0	37.0
52-53	34.82510724323258	37.0	37.0	37.0	27.0	37.0
54-55	34.81962044387467	37.0	37.0	37.0	27.0	37.0
56-57	34.78073758142541	37.0	37.0	37.0	27.0	37.0
58-59	34.76345539612883	37.0	37.0	37.0	27.0	37.0
60-61	34.74561302587534	37.0	37.0	37.0	27.0	37.0
62-63	34.732131606484444	37.0	37.0	37.0	27.0	37.0
64-65	34.68397898416142	37.0	37.0	37.0	27.0	37.0
66-67	34.643674429816635	37.0	37.0	37.0	27.0	37.0
68-69	34.60592289444418	37.0	37.0	37.0	27.0	37.0
70-71	34.54169611404215	37.0	37.0	37.0	27.0	37.0
72-73	34.48107287061234	37.0	37.0	37.0	27.0	37.0
74-75	34.40100751113522	37.0	37.0	37.0	27.0	37.0
76-77	34.3626451139271	37.0	37.0	37.0	27.0	37.0
78-79	34.310987569098614	37.0	37.0	37.0	27.0	37.0
80-81	34.26289521117192	37.0	37.0	37.0	27.0	37.0
82-83	34.222368774276966	37.0	37.0	37.0	27.0	37.0
84-85	34.17838398281917	37.0	37.0	37.0	27.0	37.0
86-87	34.11649655671154	37.0	37.0	37.0	27.0	37.0
88-89	34.07442789912836	37.0	37.0	37.0	27.0	37.0
90-91	33.97958817502972	37.0	37.0	37.0	27.0	37.0
92-93	33.918695111461744	37.0	37.0	37.0	22.0	37.0
94-95	33.87730442833741	37.0	37.0	37.0	22.0	37.0
96-97	33.79476275002877	37.0	37.0	37.0	22.0	37.0
98-99	33.704019635236044	37.0	35.0	37.0	22.0	37.0
100-101	33.62679218096851	37.0	33.0	37.0	22.0	37.0
102-103	33.513792328890204	37.0	33.0	37.0	22.0	37.0
104-105	33.393492266982236	37.0	33.0	37.0	18.0	37.0
106-107	33.32239808468791	37.0	33.0	37.0	22.0	37.0
108-109	33.22229344378154	37.0	33.0	37.0	14.0	37.0
110-111	33.15111571311956	37.0	33.0	37.0	14.0	37.0
112-113	33.00791791989218	37.0	33.0	37.0	14.0	37.0
114-115	32.904883607536334	37.0	33.0	37.0	14.0	37.0
116-117	32.71282919426502	37.0	33.0	37.0	14.0	37.0
118-119	32.64238422387675	37.0	33.0	37.0	14.0	37.0
120-121	32.530795106531016	37.0	33.0	37.0	14.0	37.0
122-123	32.47329465454804	37.0	33.0	37.0	14.0	37.0
124-125	30.958169660711448	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	678.0
1	43.0
2	27.0
3	15.0
4	14.0
5	13.0
6	22.0
7	11.0
8	16.0
9	18.0
10	10.0
11	14.0
12	10.0
13	11.0
14	28.0
15	105.0
16	204.0
17	452.0
18	887.0
19	1765.0
20	2603.0
21	3201.0
22	3265.0
23	3583.0
24	3805.0
25	4144.0
26	4628.0
27	5475.0
28	6672.0
29	7839.0
30	9236.0
31	11142.0
32	14121.0
33	18905.0
34	28423.0
35	53940.0
36	179733.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.35642412625778	20.748988689269794	12.471228175350694	23.423359009121732
2	16.548139066275578	23.963834191478657	36.555066539479725	22.932960202766044
3	19.275002198962092	29.229703579910282	28.234948104494677	23.26034611663295
4	14.082017991078668	20.270883090969555	34.444697908736366	31.202401009215414
5	14.446219104196963	39.276946803275756	31.093596654964912	15.183237437562369
6	28.22819027944134	37.51662033119788	18.442798589057503	15.812390800303286
7	25.14017125479714	34.94652751642084	21.578818688035515	18.3344825407465
8	24.928235316739784	35.412059763155945	20.36908336240547	19.290621557698806
9	26.352502252796768	19.41855864964065	19.676531352337413	34.552407745225175
10-11	24.30891416593752	28.821558718773222	25.477973849655434	21.39155326563382
12-13	25.139400734859663	27.8273821792409	25.14929413348943	21.883922952410003
14-15	23.970975600367343	27.527536885403663	23.769707501361033	24.73178001286796
16-17	23.092567882334997	29.657699057157345	24.354775983828343	22.894957076679315
18-19	23.32782387313678	28.717889533262163	25.813950612704183	22.140335980896875
20-21	25.300478209920556	27.24958546439665	25.305561165123237	22.144375160559566
22-23	25.33915953273185	27.106525489851315	24.727326175588207	22.826988801828634
24-25	24.02514012902446	27.671310393514993	25.07694906734022	23.22660041012033
26-27	23.657046787200173	27.514109782278744	25.333188652836856	23.495654777684223
28-29	23.58141636554169	27.62603642616498	25.656809544803416	23.13573766348992
30-31	24.28533757447405	27.295338865494863	25.435719524354973	22.983604035676127
32-33	23.586326997847884	27.698418257405024	25.35649981253116	23.358754932215938
34-35	23.486461025495952	27.76512852344293	25.530284512094305	23.218125938966814
36-37	23.725831901683787	27.814238492902177	25.213374147361712	23.246555458052327
38-39	23.777495200613004	27.57896673798231	25.206874864395	23.436663197009686
40-41	23.309056499603777	27.644964010498143	25.53077820001676	23.515201289881325
42-43	23.55387165392068	27.557425607969204	25.306130296085023	23.582572442025096
44-45	23.566359431816934	27.840091401452316	25.340283652103224	23.25326551462753
46-47	23.501236581952906	27.650274574749012	25.426212241680705	23.422276601617376
48-49	23.4790668296099	27.669957570681202	25.62112952613728	23.229846073571615
50-51	23.26762992603904	27.957271271672653	25.29750543103614	23.477593371252173
52-53	22.9953118434315	27.77945615186661	25.446500910441657	23.778731094260237
54-55	22.933640574511706	27.672167437287232	25.345474669768375	24.048717318432686
56-57	23.370775716463204	27.415239690757044	25.015173777515344	24.19881081526441
58-59	23.155466454288305	27.460385027325408	25.65278883915085	23.73135967923544
60-61	23.553577165892733	27.54416364370313	25.572211985489773	23.330047204914365
62-63	23.00683246188321	27.684926842128387	25.57545915901402	23.732781536974386
64-65	22.93504795955635	27.432646549191155	25.667355922243186	23.96494956900931
66-67	23.400422608889066	27.270630329807933	25.45264322775434	23.876303833548665
68-69	23.408958904598638	27.216143219444145	25.295318702533777	24.079579173423447
70-71	23.32280653576823	27.328024165865717	25.438830152409718	23.910339145956335
72-73	23.625667568198356	27.387937780584576	25.54318428314502	23.443210368072048
74-75	23.6584387907549	27.296857149134368	25.61008766148168	23.434616398629053
76-77	23.30255094613265	27.08749357937269	25.7254965514021	23.88445892309256
78-79	23.3902173107079	27.21409484995867	25.70159804247349	23.694089796859938
80-81	23.273694516881644	27.350775936293715	25.749330213384198	23.626199333440447
82-83	23.302076052822013	27.285559905363076	25.85941501420515	23.55294902760976
84-85	23.47147270854789	27.42354960521799	25.263027806385168	23.841949879848954
86-87	23.028296744412117	27.672840260490965	25.529542767778317	23.769320227318598
88-89	23.390193507643517	26.925363298759603	25.713246246885273	23.971196946711608
90-91	23.65128937271348	26.97695808251711	25.63095867280752	23.740793871961895
92-93	23.415845593938638	27.253345267889134	25.61129133449659	23.71951780367564
94-95	22.71030090050516	27.436854820997148	25.893778827146935	23.95906545135076
96-97	22.899285967238498	27.442219897932574	25.615410589427047	24.043083545401885
98-99	22.82058004395146	27.605139657749632	25.470021179606633	24.104259118692273
100-101	22.972551958309833	27.938288227083515	25.215855330607607	23.87330448399905
102-103	22.91350697728043	28.009179512448203	25.51473484687316	23.562578663398202
104-105	23.009664627408778	28.040871175361993	25.467206015376103	23.482258181853123
106-107	23.074990975608753	27.8675946806189	25.569840553075085	23.487573790697258
108-109	22.890405393534785	28.215450412860154	25.35754677617709	23.536597417427966
110-111	22.64916968965787	28.754857030702063	25.190815596850324	23.405157682789742
112-113	22.59784107987181	28.803535523363138	25.00312240514408	23.595500991620973
114-115	22.698483931485285	28.884350884704958	24.97704693952332	23.440118244286438
116-117	22.806145906960467	28.634738510736124	25.04916862815067	23.509946954152742
118-119	22.63617550350491	28.411592277670128	25.342943246254933	23.60928897257003
120-121	22.74976018575826	28.374244705217176	25.57346138064387	23.302533728380695
122-123	22.626585318326157	28.738551812909797	25.533608336832632	23.10125453193142
124-125	22.630865373267866	28.716280193104133	25.095028858583333	23.557825575044667
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1670.0
1	1011.0
2	284.0
3	192.5
4	158.5
5	132.0
6	107.0
7	98.5
8	102.5
9	100.0
10	96.5
11	101.5
12	112.0
13	115.5
14	118.0
15	125.5
16	147.5
17	189.0
18	216.0
19	246.5
20	310.5
21	367.5
22	448.0
23	578.5
24	719.0
25	841.5
26	1000.5
27	1287.0
28	1678.5
29	2247.5
30	2985.0
31	3748.5
32	4657.5
33	5502.0
34	6228.5
35	6943.0
36	7658.5
37	8612.5
38	9158.5
39	9325.0
40	9321.0
41	9230.5
42	9556.5
43	10331.0
44	11241.0
45	12239.0
46	13361.0
47	14184.5
48	15320.5
49	16321.5
50	17004.5
51	16477.0
52	14938.5
53	14585.5
54	14147.0
55	13118.5
56	12174.0
57	10778.5
58	9418.0
59	8234.5
60	7056.5
61	5880.5
62	4684.5
63	3672.5
64	2788.0
65	2128.0
66	1640.5
67	1205.0
68	960.0
69	840.5
70	686.0
71	569.5
72	446.0
73	398.0
74	344.0
75	280.5
76	222.0
77	157.5
78	111.5
79	71.5
80	44.5
81	36.5
82	28.0
83	14.5
84	6.5
85	4.0
86	3.5
87	1.0
88	1.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.5
95	1.5
96	1.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3892532145576867
2	0.3530945767521873
3	0.34241134285510794
4	0.3317281089580286
5	0.35473815119789187
6	0.2865298117011543
7	0.28461230818116573
8	0.27968158484405214
9	0.2920083931868361
10-11	0.2869407053125805
12-13	0.3229623785809378
14-15	0.3736392573234938
16-17	0.33186507349517064
18-19	0.3118682510724323
20-21	0.3003632299525007
22-23	0.2931041094839724
24-25	0.27899676215834196
26-27	0.2842014145697396
28-29	0.27570961326693294
30-31	0.2752987196555068
32-33	0.2732442515983762
34-35	0.263245840387007
36-37	0.26502637936985357
38-39	0.25995869149559797
40-41	0.2721485353012398
42-43	0.26201315955272864
44-45	0.260232620569882
46-47	0.26009565603274
48-49	0.2528365355642117
50-51	0.25941083334702975
52-53	0.25995869149559797
54-55	0.2622870886270127
56-57	0.25831511704989346
58-59	0.25420618093563213
60-61	0.2468100959299618
62-63	0.25037117389565494
64-65	0.24571437963282547
66-67	0.244207769724263
68-69	0.2513299256556492
70-71	0.24872759944995043
72-73	0.23640079110716652
74-75	0.25475403908420036
76-77	0.2731072870612341
78-79	0.2533843937127799
80-81	0.26009565603274
82-83	0.2546170745470583
84-85	0.2555758263070526
86-87	0.24639920231853568
88-89	0.2357159684214563
90-91	0.2274980961929337
92-93	0.23270274860433138
94-95	0.22407398276438265
96-97	0.21613003961014413
98-99	0.21708879137013842
100-101	0.20585769932449088
102-103	0.21051449358732038
104-105	0.21681486229585437
106-107	0.21024056451303627
108-109	0.2109253871987465
110-111	0.20996663543875219
112-113	0.20695341562162725
114-115	0.19928340154167284
116-117	0.20558377025020683
118-119	0.19503750089026947
120-121	0.1954483945016956
122-123	0.19147642292457637
124-125	0.19038070662744003
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	365058.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.9355497262679
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.16248586478106	63.704707581943744
2	9.269677474229754	13.707173996847589
3	2.1670472233990647	4.806654832343769
4	0.8184903796266444	2.420621446534306
5	0.43031273251913976	1.5907704216507539
6	0.23935966865535385	1.0618313210598551
7	0.16026248643481134	0.829436652453943
8	0.11678211275519342	0.6907479763800182
9	0.08724766400724422	0.5805633600637486
>10	0.5042834712889792	6.750047554627331
>50	0.029786814128575687	1.501909616696822
>100	0.013005510394122075	1.4901226349839847
>500	8.390651867175533E-4	0.42247023914408555
>1k	4.1953259335877663E-4	0.4429423652700104
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1428	0.39117071807767534	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	765	0.20955574182732606	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	597	0.16353565734759956	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	479	0.13121202658207737	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.369645371420432E-4	0.0
12-13	0.0	0.0	0.0	2.739290742840864E-4	0.0
14-15	0.0	0.0	0.0	2.739290742840864E-4	0.0
16-17	0.0	0.0	0.0	5.478581485681728E-4	0.0
18-19	0.0	0.0	0.0	6.848226857102159E-4	0.0
20-21	0.0	0.0	0.0	0.0013696453714204318	0.0
22-23	0.0	0.0	0.0	0.002876255279982907	0.0
24-25	0.0	0.0	0.0	0.0067112623199601165	0.0
26-27	0.0	0.0	0.0	0.014381276399914534	0.0
28-29	0.0	0.0	0.0	0.029036481874113153	0.0
30-31	0.0	0.0	0.0	0.05779903467394222	0.0
32-33	0.0	0.0	0.0	0.10121679294796991	0.0
34-35	0.0	0.0	0.0	0.15230456530195202	0.0
36-37	0.0	0.0	0.0	0.21640396868442824	0.0
38-39	0.0	0.0	0.0	0.30584181143818245	0.0
40-41	0.0	0.0	0.0	0.4111675405004136	0.0
42-43	0.0	0.0	0.0	0.5101929008541108	0.0
44-45	0.0	0.0	0.0	0.6044245024078365	0.0
46-47	0.0	0.0	0.0	0.7164614937900279	0.0
48-49	0.0	0.0	0.0	0.8389077899950145	0.0
50-51	0.0	0.0	0.0	0.9557385401771773	0.0
52-53	0.0	0.0	0.0	1.059283730256562	2.739290742840864E-4
54-55	0.0	0.0	0.0	1.1689923245073386	5.478581485681728E-4
56-57	0.0	0.0	0.0	1.2856861101523593	5.478581485681728E-4
58-59	0.0	0.0	0.0	1.4099129453401926	5.478581485681728E-4
60-61	0.0	0.0	0.0	1.5413989009965539	5.478581485681728E-4
62-63	0.0	0.0	0.0	1.661653764607268	5.478581485681728E-4
64-65	0.0	0.0	0.0	1.779580231086567	5.478581485681728E-4
66-67	0.0	0.0	0.0	1.9084638605372297	5.478581485681728E-4
68-69	0.0	0.0	0.0	2.0576182414849145	5.478581485681728E-4
70-71	0.0	0.0	0.0	2.223619260501071	5.478581485681728E-4
72-73	0.0	0.0	0.0	2.371267031540194	5.478581485681728E-4
74-75	0.0	0.0	0.0	2.5094642495165154	5.478581485681728E-4
76-77	0.0	0.0	0.0	2.6569750560184957	5.478581485681728E-4
78-79	0.0	0.0	0.0	2.819141067994675	5.478581485681728E-4
80-81	0.0	0.0	0.0	2.9954144272964847	5.478581485681728E-4
82-83	0.0	0.0	0.0	3.184288524015362	5.478581485681728E-4
84-85	0.0	0.0	0.0	3.3633011740600125	5.478581485681728E-4
86-87	0.0	0.0	0.0	3.536698278081839	5.478581485681728E-4
88-89	0.0	0.0	0.0	3.7340641761035234	5.478581485681728E-4
90-91	0.0	0.0	0.0	3.9384152655194518	5.478581485681728E-4
92-93	0.0	0.0	0.0	4.1467383265125	5.478581485681728E-4
94-95	0.0	0.0	0.0	4.3583485363969565	5.478581485681728E-4
96-97	0.0	0.0	0.0	4.564891058407158	5.478581485681728E-4
98-99	0.0	0.0	0.0	4.792252190062949	5.478581485681728E-4
100-101	0.0	0.0	0.0	5.038651392381484	5.478581485681728E-4
102-103	0.0	0.0	0.0	5.302445090917059	5.478581485681728E-4
104-105	0.0	0.0	0.0	5.55651430731555	6.848226857102159E-4
106-107	0.0	0.0	0.0	5.813870672605448	8.217872228522591E-4
108-109	0.0	0.0	0.0	6.083553846238132	8.217872228522591E-4
110-111	0.0	0.0	0.0	6.367481331733588	8.217872228522591E-4
112-113	0.0	0.0	0.0	6.651134888154759	8.217872228522591E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAAT	85	0.0071472945	28.045357	1
TACGTCG	45	0.009502631	26.45818	22-23
TAGACTT	125	0.0016199751	23.828724	4
CATGGGG	1770	0.0	23.559475	4
GTATAAG	180	2.1447446E-5	23.17637	1
GGTATCA	2530	0.0	22.613647	1
GGGTAAA	160	2.5781183E-4	22.348644	1
GTGTTAT	135	0.0025280837	22.072735	1
CCGATCG	200	4.8263682E-5	20.864471	9
ATGGGGG	1045	0.0	19.95228	5
GTATTAT	150	0.0046560233	19.865461	1
GTATCCA	180	5.7105924E-4	19.865461	1
ATAAGGT	210	7.062974E-5	19.85727	3
GTATCAA	3110	0.0	19.162823	1
TATGTAA	160	0.006776567	18.61619	2
TGGGGGG	755	0.0	18.137732	6
GTAAGTA	165	0.008092585	18.052063	5
ATGGGGA	465	1.4551915E-10	17.9356	5
AGGGCTA	235	1.6736376E-4	17.744795	5
GTACATG	4500	0.0	17.084297	1
>>END_MODULE