FastQCFastQC Report
Sun 1 Jan 2017
ERR1142470_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142470_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365058
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23670.6483901188304325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20600.5642938930252179No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12900.3533685058264714No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6650.18216283439891742No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6030.16517923179330407No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5490.15038706178196343No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5100.13970382788488406No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG4150.11368056582789583No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3770.10327126100510056No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20650.035.4445951
GTATCAA29150.033.4788321
CGCATAG800.00535457129.753291
GTGTTAT1454.053407E-628.7273161
TATCAAC35450.027.5215912
GTACTAG1306.361738E-527.4645751
ATCAACG35300.027.3014853
CAACGCA36600.026.9819265
TCAACGC36800.026.8352854
CGGGGGA450.00949617426.46187692-93
AACGCAG38350.026.0573586
CCCCTAA1150.001002848425.8724251
CATGGGT2851.1641532E-1025.0485344
ACGCAGA40950.024.2576687
CGCAGAG41950.023.676178
GAGTACT19550.023.27242512-13
CTCAACG1802.1699258E-523.1414491
GCAGAGT44700.022.2195859
TATATGT1653.2130152E-421.6328263
TGGGTAC2204.1753865E-621.6298646
Produced by FastQC (version 0.11.5)