##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142470_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	365058
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.89829013471832	33.0	14.0	33.0	14.0	33.0
2	30.24910288228172	33.0	27.0	33.0	27.0	33.0
3	29.686597746111577	33.0	27.0	33.0	27.0	33.0
4	30.83440987459527	33.0	33.0	33.0	27.0	33.0
5	31.736710330961106	33.0	33.0	33.0	27.0	33.0
6	33.8368670183916	37.0	33.0	37.0	27.0	37.0
7	35.48688701521402	37.0	37.0	37.0	33.0	37.0
8	35.23892641717207	37.0	37.0	37.0	33.0	37.0
9	35.81669214207057	37.0	37.0	37.0	33.0	37.0
10-11	35.93530343123558	37.0	37.0	37.0	33.0	37.0
12-13	36.00445271710249	37.0	37.0	37.0	33.0	37.0
14-15	36.08709027058714	37.0	37.0	37.0	37.0	37.0
16-17	36.11981383780112	37.0	37.0	37.0	37.0	37.0
18-19	36.1512814402095	37.0	37.0	37.0	37.0	37.0
20-21	36.18017547896498	37.0	37.0	37.0	37.0	37.0
22-23	36.18034668463641	37.0	37.0	37.0	37.0	37.0
24-25	36.17491330144799	37.0	37.0	37.0	37.0	37.0
26-27	36.13590032268845	37.0	37.0	37.0	37.0	37.0
28-29	36.11146447961694	37.0	37.0	37.0	37.0	37.0
30-31	36.03646680801407	37.0	37.0	37.0	37.0	37.0
32-33	35.97600244344734	37.0	37.0	37.0	37.0	37.0
34-35	35.8964602885021	37.0	37.0	37.0	35.0	37.0
36-37	35.81365974721825	37.0	37.0	37.0	33.0	37.0
38-39	35.75186956593199	37.0	37.0	37.0	33.0	37.0
40-41	35.6103591758022	37.0	37.0	37.0	33.0	37.0
42-43	35.559072804869366	37.0	37.0	37.0	33.0	37.0
44-45	35.46416870743827	37.0	37.0	37.0	33.0	37.0
46-47	35.297929370127484	37.0	37.0	37.0	33.0	37.0
48-49	35.150634967594186	37.0	37.0	37.0	33.0	37.0
50-51	35.065655320524414	37.0	37.0	37.0	33.0	37.0
52-53	34.99076174196977	37.0	37.0	37.0	30.0	37.0
54-55	34.94140246207452	37.0	37.0	37.0	27.0	37.0
56-57	34.89005171780923	37.0	37.0	37.0	27.0	37.0
58-59	34.843098904831564	37.0	37.0	37.0	27.0	37.0
60-61	34.816308641366575	37.0	37.0	37.0	27.0	37.0
62-63	34.80393800437191	37.0	37.0	37.0	27.0	37.0
64-65	34.78740364544812	37.0	37.0	37.0	27.0	37.0
66-67	34.738912720718346	37.0	37.0	37.0	27.0	37.0
68-69	34.71091032109966	37.0	37.0	37.0	27.0	37.0
70-71	34.67477907620159	37.0	37.0	37.0	27.0	37.0
72-73	34.64434829533937	37.0	37.0	37.0	27.0	37.0
74-75	34.54535854576533	37.0	37.0	37.0	27.0	37.0
76-77	34.49802497137441	37.0	37.0	37.0	27.0	37.0
78-79	34.48236307655222	37.0	37.0	37.0	27.0	37.0
80-81	34.44727961036328	37.0	37.0	37.0	27.0	37.0
82-83	34.39564261021536	37.0	37.0	37.0	27.0	37.0
84-85	34.31779881553068	37.0	37.0	37.0	27.0	37.0
86-87	34.145552213620846	37.0	37.0	37.0	27.0	37.0
88-89	34.06747968815914	37.0	37.0	37.0	27.0	37.0
90-91	34.027190199913434	37.0	37.0	37.0	27.0	37.0
92-93	33.973237129442445	37.0	37.0	37.0	27.0	37.0
94-95	34.00654142629391	37.0	37.0	37.0	27.0	37.0
96-97	33.97154013882725	37.0	37.0	37.0	22.0	37.0
98-99	33.90713667417232	37.0	37.0	37.0	22.0	37.0
100-101	33.852552197185105	37.0	37.0	37.0	22.0	37.0
102-103	33.75640172246602	37.0	37.0	37.0	22.0	37.0
104-105	33.66238515523561	37.0	35.0	37.0	22.0	37.0
106-107	33.58178152512751	37.0	33.0	37.0	22.0	37.0
108-109	33.48726914627265	37.0	33.0	37.0	18.0	37.0
110-111	33.40935139073791	37.0	33.0	37.0	14.0	37.0
112-113	33.3550682357324	37.0	33.0	37.0	14.0	37.0
114-115	33.244826849432144	37.0	33.0	37.0	14.0	37.0
116-117	33.13090796530962	37.0	33.0	37.0	14.0	37.0
118-119	32.99170679727605	37.0	33.0	37.0	14.0	37.0
120-121	32.9061984670929	37.0	33.0	37.0	14.0	37.0
122-123	32.78810490387829	37.0	33.0	37.0	14.0	37.0
124-125	31.575666058544122	37.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	4.0
16	7.0
17	8.0
18	48.0
19	285.0
20	1069.0
21	2626.0
22	4050.0
23	5366.0
24	4565.0
25	3161.0
26	2956.0
27	3323.0
28	4021.0
29	5191.0
30	6802.0
31	9291.0
32	13165.0
33	19524.0
34	32100.0
35	67791.0
36	179705.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.63244481466056	16.78631710471514	13.270512286547273	25.310725794077026
2	17.13070251850391	21.17690887475415	38.447315221142944	23.245073385599
3	20.154112919352322	26.215084219724595	29.38856123838195	24.242241622541137
4	14.09008979395055	17.79388480734568	36.19753573404774	31.918489664656025
5	14.940308892577923	35.16241788706081	33.27909359368408	16.618179626677186
6	28.519106971647716	35.18093411861389	19.32831119024791	16.97164771949048
7	24.920140924557145	32.79208148639245	23.608150830917925	18.679626758132475
8	25.577579453127996	32.004777323055514	21.947197431641	20.47044579217549
9	26.99269705087959	15.931988889436747	21.528633806134913	35.54668025354875
10-11	24.894948200012053	25.471568901380053	27.28114436610073	22.35233853250716
12-13	26.354442307797665	24.581984232642483	27.285664195826413	21.777909263733434
14-15	24.484602446734492	24.44419790827759	26.683568090549997	24.387631554437924
16-17	22.680505563499498	26.86682664124605	27.379621868305858	23.073045926948595
18-19	22.586547891020057	25.286392847164013	30.356546083088165	21.770513178727764
20-21	24.12314822931276	24.12150464586255	30.094971730364655	21.660375394460026
22-23	23.942496808726286	24.040837346394273	29.891688444028077	22.12497740085137
24-25	23.044009445074483	24.70922428764744	30.0168740309759	22.229892236302177
26-27	22.647360145511126	24.77209101019564	30.154112497192227	22.42643634710101
28-29	22.39986522689545	24.64019416092785	30.618011384492327	22.341929227684368
30-31	23.219597981690583	24.361334363306653	30.29326846692854	22.125799188074225
32-33	22.504232204197688	24.710183039407436	30.14890784478083	22.636676911614046
34-35	22.633557727207357	24.751169336337426	30.28851619265458	22.326756743800637
36-37	22.76408510987995	24.76377009101306	29.902275668901474	22.569869130205518
38-39	22.853765702984184	24.57582082847109	29.85059908288546	22.719814385659266
40-41	22.490193070500318	24.659496833293158	30.017970239530143	22.83233985667638
42-43	22.94063264006355	24.685426268464557	29.669983495750667	22.70395759572122
44-45	22.875953958001194	24.761681705372844	29.434226889973647	22.928137446652315
46-47	22.59470446672794	24.76513073099507	29.43197180609982	23.208192996177168
48-49	22.888846578993867	24.875935364482242	29.31733288770969	22.9178851688142
50-51	22.68064252803664	25.323510236729508	28.838705082480043	23.15714215275381
52-53	22.670918045899555	25.170246919667562	28.617233425921363	23.541601608511524
54-55	22.46177319768366	25.21188413895874	28.523412717979063	23.802929945378544
56-57	22.79637756192167	25.07889157339382	28.197848013192424	23.926882851492092
58-59	22.578466983328678	25.10655840989651	28.65544653178399	23.659528074990824
60-61	22.94296104038405	25.03989097607911	28.911061955993233	23.106086027543608
62-63	22.40643952467827	25.182984621621767	28.88691659955404	23.52365925414592
64-65	22.12894937242849	24.99986303546286	29.115373447506972	23.75581414460168
66-67	22.645504964653636	24.967913640093087	28.80197682111495	23.584604574138325
68-69	22.48149609103211	24.88316924981784	28.86719370620559	23.768140952944464
70-71	22.426025453489583	24.986440510822938	29.001829846216214	23.58570418947126
72-73	22.715157591396434	25.203803231267358	29.07510587358721	23.005933303748993
74-75	22.644902703680756	25.04756526173231	29.222342239597328	23.08518979498961
76-77	22.382744199558218	24.988489555642033	29.125261040434548	23.5035052043652
78-79	22.383521454898077	25.184868643620916	29.116862732207206	23.314747169273797
80-81	22.257148179193443	25.47471908573432	29.00388431427335	23.264248420798886
82-83	22.454514077215126	25.225580592672948	29.16618729078667	23.15371803932526
84-85	22.384966771147845	25.44909317921103	28.709481564120242	23.45645848552088
86-87	22.200500544338535	25.65749642713483	28.648955872197824	23.49304715632881
88-89	22.361673784967795	25.123116854580662	28.856997679113395	23.65821168133815
90-91	22.715998573270777	24.961450873871648	28.64707657694735	23.675473975910226
92-93	22.55974217437515	25.248534302465114	28.475880275653992	23.715843247505745
94-95	21.85993381837307	25.347480934431978	28.757642663043477	24.034942584151473
96-97	22.083888039708757	25.331591144420884	28.336593089317315	24.24792772655304
98-99	22.070739444143122	25.4518460080316	28.074716894301726	24.40269765352355
100-101	22.360556404735686	25.669208728476022	27.735318771263746	24.234916095524547
102-103	22.377129113729875	25.60784861583639	28.069786170964612	23.945236099469124
104-105	22.504222007031885	25.820192493387918	27.753070457182066	23.922515042398125
106-107	22.449164080241722	25.69066448977803	27.748070796363205	24.112100633617043
108-109	22.39244476708351	25.844895834760095	27.619334602583244	24.143324795573147
110-111	22.063905351378075	26.360375340187996	27.57210889850995	24.00361040992397
112-113	22.044990111160416	26.303765429055108	27.286759912123554	24.36448454766092
114-115	22.019240778177714	26.46935555445984	27.28456847952928	24.226835187833164
116-117	22.15955316567002	26.178514588132813	27.3866820797848	24.27525016641237
118-119	21.965824608692316	26.09790772973062	27.43536643492267	24.500901226654396
120-121	22.063798605689552	26.060182712194052	27.748421470737288	24.127597211379108
122-123	21.95459351664667	26.501131327076795	27.66327542472703	23.880999731549508
124-125	21.762432271036385	26.326775471294972	27.619309808304433	24.29148244936421
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	158.0
1	160.5
2	288.0
3	398.0
4	287.0
5	233.5
6	360.0
7	736.5
8	1178.5
9	1358.5
10	1179.0
11	894.0
12	826.5
13	793.5
14	767.0
15	750.0
16	729.5
17	734.0
18	733.0
19	741.0
20	757.0
21	770.0
22	784.5
23	826.0
24	855.0
25	930.0
26	1076.5
27	1243.0
28	1498.5
29	1978.5
30	2712.5
31	3289.0
32	4094.0
33	5079.5
34	5759.5
35	6378.5
36	7005.0
37	7942.0
38	8620.5
39	8761.5
40	8882.0
41	8949.0
42	9276.0
43	9891.0
44	10780.0
45	11768.5
46	12802.0
47	13898.0
48	14962.5
49	15812.0
50	16918.5
51	16628.5
52	14749.0
53	14111.0
54	13875.0
55	13190.5
56	12295.5
57	10828.0
58	9446.0
59	8400.5
60	7197.0
61	5913.0
62	4731.0
63	3710.5
64	2794.5
65	2027.0
66	1475.0
67	1159.5
68	949.5
69	780.0
70	646.5
71	542.0
72	454.0
73	405.5
74	325.0
75	277.5
76	214.0
77	136.5
78	91.5
79	55.5
80	45.0
81	35.0
82	21.5
83	9.0
84	5.0
85	3.0
86	2.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029036481874113153
2	0.0
3	2.739290742840864E-4
4	0.0
5	0.003287148891409036
6	0.002191432594272691
7	0.010957162971363454
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.478581485681728E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	1.369645371420432E-4
36-37	1.369645371420432E-4
38-39	0.0
40-41	0.0027392907428408636
42-43	1.369645371420432E-4
44-45	0.0
46-47	0.003971971577119252
48-49	0.007259120468528288
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	1.369645371420432E-4
62-63	0.0
64-65	0.0
66-67	0.007533049542812375
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.08108300598808957
76-77	0.04711580077686286
78-79	4.108936114261295E-4
80-81	0.0
82-83	0.0
84-85	0.06560601329103868
86-87	0.23407239397575183
88-89	0.26653298927841607
90-91	0.16107029567904277
92-93	0.12888362945066265
94-95	5.478581485681728E-4
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0017805389828465612
106-107	0.00301321981712495
108-109	0.0035610779656931224
110-111	6.848226857102159E-4
112-113	0.0
114-115	0.0
116-117	2.739290742840864E-4
118-119	0.0
120-121	8.21787222852259E-4
122-123	0.0
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	365058.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.3514627234054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.34693634158985	58.49583411527218
2	10.402150687027262	14.428087312292345
3	2.4861279054651706	5.172498202844573
4	0.8793388891745031	2.439337527753051
5	0.48037952989128063	1.665751153017108
6	0.3064542009140155	1.2751828254671602
7	0.19151087015981166	0.9297091281110578
8	0.13998524074704005	0.7766544964396225
9	0.11610864856593109	0.7247074151596634
>10	0.5907106625478517	7.610623273515508
>50	0.03990244229825333	1.9398722104732473
>100	0.01729105832922678	2.068397584378575
>500	0.0017734418799206953	0.7154989116766678
>1k	0.0013300814099405215	1.7578458435992736
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2367	0.6483901188304325	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2060	0.5642938930252179	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1290	0.3533685058264714	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	665	0.18216283439891742	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	603	0.16517923179330407	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	549	0.15038706178196343	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	510	0.13970382788488406	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	415	0.11368056582789583	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	377	0.10327126100510056	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.369645371420432E-4	0.0
12-13	0.0	0.0	0.0	2.739290742840864E-4	0.0
14-15	0.0	0.0	0.0	2.739290742840864E-4	0.0
16-17	0.0	0.0	0.0	5.478581485681728E-4	8.217872228522591E-4
18-19	0.0	0.0	0.0	6.848226857102159E-4	8.217872228522591E-4
20-21	0.0	0.0	0.0	0.0013696453714204318	8.217872228522591E-4
22-23	0.0	0.0	0.0	0.002876255279982907	8.217872228522591E-4
24-25	0.0	0.0	0.0	0.0067112623199601165	8.217872228522591E-4
26-27	0.0	0.0	0.0	0.014518240937056576	8.217872228522591E-4
28-29	0.0	0.0	0.0	0.029995233634107457	8.217872228522591E-4
30-31	0.0	0.0	0.0	0.05903171550822062	8.217872228522591E-4
32-33	0.0	0.0	0.0	0.10217554470796422	8.217872228522591E-4
34-35	0.0	0.0	0.0	0.15271545891337815	8.217872228522591E-4
36-37	0.0	0.0	0.0	0.21681486229585434	8.217872228522591E-4
38-39	0.0	0.0	0.0	0.30597877597532447	8.217872228522591E-4
40-41	0.0	0.0	0.0	0.41144146957469774	8.217872228522591E-4
42-43	0.0	0.0	0.0	0.5111516526141051	8.217872228522591E-4
44-45	0.0	0.0	0.0	0.6067528995392513	8.217872228522591E-4
46-47	0.0	0.0	0.0	0.720159536292863	8.217872228522591E-4
48-49	0.0	0.0	0.0	0.843427619720702	8.217872228522591E-4
50-51	0.0	0.0	0.0	0.9605322989771489	9.587517599943024E-4
52-53	0.0	0.0	0.0	1.0640774890565337	0.0012326808342783887
54-55	0.0	0.0	0.0	1.1740600123815943	0.0013696453714204318
56-57	0.0	0.0	0.0	1.2933561242323137	0.0013696453714204318
58-59	0.0	0.0	0.0	1.4163502785858686	0.0013696453714204318
60-61	0.0	0.0	0.0	1.5493428441507926	0.0013696453714204318
62-63	0.0	0.0	0.0	1.6705564595215008	0.0013696453714204318
64-65	0.0	0.0	0.0	1.7882089969265158	0.0013696453714204318
66-67	0.0	0.0	0.0	1.9172295909143204	0.0013696453714204318
68-69	0.0	0.0	0.0	2.065425220102011	0.0013696453714204318
70-71	0.0	0.0	0.0	2.232521955415304	0.0013696453714204318
72-73	0.0	0.0	0.0	2.3814024072887046	0.0013696453714204318
74-75	0.0	0.0	0.0	2.5191887316536006	0.0013696453714204318
76-77	0.0	0.0	0.0	2.667247396304149	0.0013696453714204318
78-79	0.0	0.0	0.0	2.832015734486027	0.0013696453714204318
80-81	0.0	0.0	0.0	3.0081521292506945	0.0013696453714204318
82-83	0.0	0.0	0.0	3.1963414032838617	0.0013696453714204318
84-85	0.0	0.0	0.0	3.3739844079570918	0.0013696453714204318
86-87	0.0	0.0	0.0	3.546559724756066	0.0013696453714204318
88-89	0.0	0.0	0.0	3.7446104454634606	0.0013696453714204318
90-91	0.0	0.0	0.0	3.948276712193679	0.0013696453714204318
92-93	0.0	0.0	0.0	4.158243347632431	0.0013696453714204318
94-95	0.0	0.0	0.0	4.36999052205403	0.0016435744457045183
96-97	0.0	0.0	0.0	4.578861441195645	0.0016435744457045183
98-99	0.0	0.0	0.0	4.806907395537148	0.0016435744457045183
100-101	0.0	0.0	0.0	5.0538544560042515	0.0016435744457045183
102-103	0.0	0.0	0.0	5.320935303431235	0.0016435744457045183
104-105	0.0	0.0	0.0	5.578291668721135	0.0016435744457045183
106-107	0.0	0.0	0.0	5.839620005588153	0.0016435744457045183
108-109	0.0	0.0	0.0	6.1101249664436885	0.0016435744457045183
110-111	0.0	0.0	0.0	6.3944633455505695	0.0016435744457045183
112-113	0.0	0.0	0.0	6.681404050863151	0.0016435744457045183
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2065	0.0	35.444595	1
GTATCAA	2915	0.0	33.478832	1
CGCATAG	80	0.005354571	29.75329	1
GTGTTAT	145	4.053407E-6	28.727316	1
TATCAAC	3545	0.0	27.521591	2
GTACTAG	130	6.361738E-5	27.464575	1
ATCAACG	3530	0.0	27.301485	3
CAACGCA	3660	0.0	26.981926	5
TCAACGC	3680	0.0	26.835285	4
CGGGGGA	45	0.009496174	26.461876	92-93
AACGCAG	3835	0.0	26.057358	6
CCCCTAA	115	0.0010028484	25.872425	1
CATGGGT	285	1.1641532E-10	25.048534	4
ACGCAGA	4095	0.0	24.257668	7
CGCAGAG	4195	0.0	23.67617	8
GAGTACT	1955	0.0	23.272425	12-13
CTCAACG	180	2.1699258E-5	23.141449	1
GCAGAGT	4470	0.0	22.219585	9
TATATGT	165	3.2130152E-4	21.632826	3
TGGGTAC	220	4.1753865E-6	21.629864	6
>>END_MODULE