FastQCFastQC Report
Sun 1 Jan 2017
ERR1142469_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142469_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389147
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9020.23178901546202338No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8000.205577840764544No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4620.11872120304152414No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGGC750.003891060131.760383
ATAGACT1552.090674E-423.0518884
CATGGGG15150.022.4052184
GGTAAAC1350.00254156922.0529778
GTATCAA26050.021.95991
TAGACTG1653.1915432E-421.6548045
GTACATA1953.9838746E-521.390961
TATCAAC29000.019.9186862
TATTCAC1500.00467727519.8502372
TATTCTC2107.083793E-519.8502355
AACGCAG27700.019.7683876
ATCAACG28400.019.2910753
CAACGCA28250.019.1827075
TCAACGC28800.018.8163724
CCAGTAC1908.2606915E-418.8054873
GTCCTAC1600.006766010518.6215951
TGTTAAG1600.006791232618.6095962
ACATGGG37250.018.0650483
GTACAAG3303.6918027E-718.0573041
ACGCAGA30350.018.0469657
Produced by FastQC (version 0.11.5)