##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142469_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	389147
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.169444965527166	33.0	33.0	33.0	27.0	33.0
2	31.235674436652474	33.0	33.0	33.0	27.0	33.0
3	31.354043587641687	33.0	33.0	33.0	27.0	33.0
4	31.300940261649195	33.0	33.0	33.0	27.0	33.0
5	31.3528640847803	33.0	33.0	33.0	27.0	33.0
6	34.69700652966617	37.0	37.0	37.0	27.0	37.0
7	34.685640644794894	37.0	37.0	37.0	27.0	37.0
8	34.69791107216553	37.0	37.0	37.0	27.0	37.0
9	34.63813931496324	37.0	37.0	37.0	27.0	37.0
10-11	34.72188016353717	37.0	37.0	37.0	27.0	37.0
12-13	34.65827823418914	37.0	37.0	37.0	27.0	37.0
14-15	34.638003119643734	37.0	37.0	37.0	27.0	37.0
16-17	34.606863216213924	37.0	37.0	37.0	27.0	37.0
18-19	34.65189889681791	37.0	37.0	37.0	27.0	37.0
20-21	34.643831251429404	37.0	37.0	37.0	27.0	37.0
22-23	34.71828769076981	37.0	37.0	37.0	27.0	37.0
24-25	34.789657892775736	37.0	37.0	37.0	27.0	37.0
26-27	34.83282666961328	37.0	37.0	37.0	27.0	37.0
28-29	34.87493158112487	37.0	37.0	37.0	27.0	37.0
30-31	34.82537447288557	37.0	37.0	37.0	27.0	37.0
32-33	34.84269183624697	37.0	37.0	37.0	27.0	37.0
34-35	34.90679100699735	37.0	37.0	37.0	27.0	37.0
36-37	34.88908561546151	37.0	37.0	37.0	27.0	37.0
38-39	34.86315454057207	37.0	37.0	37.0	27.0	37.0
40-41	34.839844583152384	37.0	37.0	37.0	27.0	37.0
42-43	34.85257884552624	37.0	37.0	37.0	27.0	37.0
44-45	34.843781398803024	37.0	37.0	37.0	27.0	37.0
46-47	34.825293526610764	37.0	37.0	37.0	27.0	37.0
48-49	34.83606966005134	37.0	37.0	37.0	27.0	37.0
50-51	34.764215065258114	37.0	37.0	37.0	27.0	37.0
52-53	34.78493217216116	37.0	37.0	37.0	27.0	37.0
54-55	34.78355223090503	37.0	37.0	37.0	27.0	37.0
56-57	34.750740722657504	37.0	37.0	37.0	27.0	37.0
58-59	34.71197002675082	37.0	37.0	37.0	27.0	37.0
60-61	34.714228813276215	37.0	37.0	37.0	27.0	37.0
62-63	34.67577033871519	37.0	37.0	37.0	27.0	37.0
64-65	34.63633536941053	37.0	37.0	37.0	27.0	37.0
66-67	34.59603697317466	37.0	37.0	37.0	27.0	37.0
68-69	34.55343096567621	37.0	37.0	37.0	27.0	37.0
70-71	34.495995086689604	37.0	37.0	37.0	27.0	37.0
72-73	34.43186122467859	37.0	37.0	37.0	27.0	37.0
74-75	34.347859549219194	37.0	37.0	37.0	27.0	37.0
76-77	34.3048667984078	37.0	37.0	37.0	27.0	37.0
78-79	34.25040151922025	37.0	37.0	37.0	27.0	37.0
80-81	34.205022780594476	37.0	37.0	37.0	27.0	37.0
82-83	34.158554479412665	37.0	37.0	37.0	27.0	37.0
84-85	34.10232251565604	37.0	37.0	37.0	27.0	37.0
86-87	34.03232711546022	37.0	37.0	37.0	27.0	37.0
88-89	33.99616982785425	37.0	37.0	37.0	27.0	37.0
90-91	33.90842920541595	37.0	37.0	37.0	24.5	37.0
92-93	33.84002446376305	37.0	37.0	37.0	22.0	37.0
94-95	33.76450801368121	37.0	37.0	37.0	22.0	37.0
96-97	33.690331674148844	37.0	33.0	37.0	22.0	37.0
98-99	33.600084286914715	37.0	33.0	37.0	22.0	37.0
100-101	33.51727881751626	37.0	33.0	37.0	22.0	37.0
102-103	33.40173122239153	37.0	33.0	37.0	22.0	37.0
104-105	33.262411119705405	37.0	33.0	37.0	18.0	37.0
106-107	33.2105412607575	37.0	33.0	37.0	14.0	37.0
108-109	33.09914762287773	37.0	33.0	37.0	14.0	37.0
110-111	33.02788406437671	37.0	33.0	37.0	14.0	37.0
112-113	32.88932202997839	37.0	33.0	37.0	14.0	37.0
114-115	32.78396210172505	37.0	33.0	37.0	14.0	37.0
116-117	32.57865536673802	37.0	33.0	37.0	14.0	37.0
118-119	32.48560312683896	37.0	33.0	37.0	14.0	37.0
120-121	32.39795886901351	37.0	33.0	37.0	14.0	37.0
122-123	32.329709595602694	37.0	33.0	37.0	14.0	37.0
124-125	30.743418296941776	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	718.0
1	40.0
2	27.0
3	21.0
4	16.0
5	12.0
6	22.0
7	17.0
8	19.0
9	19.0
10	20.0
11	9.0
12	11.0
13	18.0
14	27.0
15	171.0
16	246.0
17	443.0
18	928.0
19	1742.0
20	2522.0
21	3059.0
22	3471.0
23	3720.0
24	4104.0
25	4471.0
26	5135.0
27	6063.0
28	7259.0
29	8709.0
30	10101.0
31	12391.0
32	15832.0
33	21016.0
34	31800.0
35	59879.0
36	185089.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.79209563043809	19.90821830526313	12.058535981365067	24.241150082933714
2	16.888428175057506	23.3855246464709	36.21545935575703	23.51058782271457
3	19.461382768889635	28.33760909641738	28.20611326689786	23.994894867795125
4	14.280631646793426	19.48797937479858	34.42423461166613	31.807154366741862
5	14.964646178125498	38.1202390960149	31.45071868053657	15.464396045323031
6	29.1927802746052	36.64108563889003	18.329948872304456	15.836185214200304
7	25.84877119834253	33.83617524049054	21.81641546045596	18.49863810071097
8	25.352373982818065	34.524404636133596	20.551326781453405	19.57189459959493
9	26.220492732707967	18.701680239150605	19.881455520049478	35.19637150809195
10-11	24.643892080778354	27.808694509334504	26.000548899045096	21.546864510842045
12-13	25.41859946562593	26.528463216993764	25.528155172124904	22.5247821452554
14-15	24.115315278139562	26.74338533593647	23.62980577040623	25.511493615517733
16-17	23.585866398535593	28.781808337845156	24.328769948694152	23.303555314925102
18-19	23.665006624701633	27.92853055353635	25.813781441555694	22.59268138020632
20-21	25.250171721220106	26.81763528603665	25.22426855781238	22.707924434930867
22-23	25.192140165670956	26.58248238392981	24.811596966994674	23.413780483404555
24-25	24.233830390632146	27.045835529138607	25.152911798850923	23.567422281378324
26-27	24.054932339623907	26.873062382583285	25.208676402751255	23.863328875041557
28-29	23.902027353488762	26.9808731728372	25.521333273206338	23.5957662004677
30-31	24.38516247068828	26.892957456129047	25.364238410596023	23.357641662586648
32-33	23.840795096868288	27.115132634247967	25.280581119541534	23.763491149342208
34-35	23.90694666489746	27.137120721217457	25.490986714211918	23.46494589967317
36-37	24.04456978102886	27.286172365859723	25.055750875072953	23.61350697803847
38-39	24.048014098151164	27.044901787002495	25.179705051837498	23.72737906300884
40-41	23.697609342066613	27.021417491638623	25.462130308636567	23.818842857658197
42-43	23.961048079264245	27.06202599972431	25.329827540229232	23.647098380782207
44-45	23.81394653822719	27.107161857700635	25.32896728425994	23.749924319812234
46-47	23.724062595002913	27.114281739345504	25.30181941846625	23.85983624718534
48-49	23.7618533622202	27.328359708486506	25.417653105123637	23.492133824169652
50-51	23.47349017193162	27.47402089664136	25.353440757854273	23.69904817357274
52-53	23.464883184402087	27.160447708322334	25.325353692923823	24.04931541435176
54-55	23.423372360693705	26.981049680983855	25.347903433368586	24.24767452495385
56-57	23.77260249620957	26.858663630028197	25.05857938392136	24.310154489840873
58-59	23.595323261454517	27.0225013621381	25.486859714588594	23.895315661818785
60-61	23.724047857946033	27.048832982775213	25.490062224603644	23.737056934675113
62-63	23.36285967111	27.170198707058113	25.49985895881984	23.967082663012047
64-65	23.301511084549638	26.9927974899407	25.58423794043246	24.1214534850772
66-67	23.682437871499136	26.837709214788486	25.31751704951605	24.162335864196336
68-69	23.63182958135988	26.847659616125263	25.307626615418105	24.21288418709675
70-71	23.513349201933085	26.915437880614547	25.398647047306977	24.172565870145394
72-73	23.923251483016468	26.878594849946808	25.45701119699971	23.741142470037012
74-75	23.87579444717812	26.740183891818642	25.506939095173937	23.8770825658293
76-77	23.843007414182928	26.62020187705726	25.5364815160748	24.000309192685016
78-79	23.70997243475797	26.660354999098335	25.60115413349821	24.02851843264549
80-81	23.617158315084374	26.906479453819397	25.564343681566402	23.91201854952982
82-83	23.776131899272237	26.690281445224446	25.719198814967477	23.81438784053584
84-85	23.726839924980936	26.890289771439168	25.409874075142724	23.97299622843717
86-87	23.453735391971104	26.99366668770472	25.53431284785238	24.018285072471794
88-89	23.599761480674395	26.469334425473654	25.667229479546194	24.26367461430575
90-91	23.7782121912209	26.590223637070746	25.53732907848546	24.094235093222892
92-93	23.653522458141712	26.869279539053352	25.53030371325286	23.946894289552073
94-95	23.209132525931505	26.964259269990276	25.65207033538725	24.174537868690965
96-97	23.360185008730227	26.946274330038683	25.47076274896602	24.22277791226507
98-99	23.21785206121516	27.179325397080884	25.388968652017436	24.213853889686522
100-101	23.36307933569121	27.329542016281977	25.299251822162596	24.008126825864213
102-103	23.432345783923676	27.39284380753731	25.4590752829387	23.715735125600318
104-105	23.435309023812067	27.415876171578745	25.51212106775102	23.636693736858167
106-107	23.330058622896278	27.396064424670286	25.508234630642278	23.76564232179116
108-109	23.29941454357704	27.414862893496373	25.36814741089719	23.917575152029396
110-111	23.13418038359325	28.014590758328012	25.26511093714592	23.58611792093282
112-113	23.028640954827246	27.94903982953192	25.113784867739174	23.908534347901657
114-115	23.057325004763353	28.049703642290325	25.101060296306215	23.79191105664011
116-117	23.231280255433102	27.96644865588629	25.046863734679164	23.75540735400144
118-119	22.988817969520404	27.731461336642717	25.293068068003638	23.986652625833248
120-121	23.110879956538362	27.896680771959005	25.441535987806024	23.55090328369661
122-123	23.000933286132653	28.12164902004956	25.35397290251987	23.523444791297912
124-125	23.03007600098863	27.9779926676553	25.14301676552974	23.84891456582633
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1307.0
1	791.5
2	220.5
3	145.5
4	123.5
5	108.5
6	83.0
7	75.0
8	79.0
9	79.5
10	77.5
11	74.5
12	80.5
13	98.5
14	103.5
15	116.0
16	134.5
17	154.5
18	190.5
19	230.5
20	271.0
21	322.0
22	397.5
23	503.5
24	652.0
25	777.5
26	940.0
27	1188.0
28	1480.0
29	1991.5
30	2722.5
31	3378.0
32	4196.5
33	5137.0
34	5942.5
35	6774.5
36	7652.0
37	8660.5
38	9283.0
39	9676.0
40	10041.0
41	10285.5
42	10625.5
43	11416.5
44	12468.5
45	13410.0
46	14521.0
47	15302.5
48	16397.5
49	17212.5
50	17508.0
51	17208.5
52	16056.5
53	15579.0
54	15187.0
55	14279.0
56	13228.5
57	11870.5
58	10591.0
59	9388.5
60	8040.5
61	6779.5
62	5605.0
63	4370.5
64	3266.0
65	2524.0
66	1965.5
67	1527.5
68	1238.5
69	1061.0
70	887.5
71	758.5
72	637.5
73	561.0
74	469.5
75	368.0
76	284.0
77	202.5
78	137.5
79	103.0
80	76.5
81	45.5
82	33.0
83	17.5
84	13.0
85	11.5
86	5.5
87	3.0
88	2.0
89	2.0
90	1.5
91	1.0
92	1.5
93	1.0
94	1.5
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3823747838220518
2	0.3451138001834782
3	0.33457793584429535
4	0.32686876681562493
5	0.347683523193035
6	0.2824125587502923
7	0.27907191883786847
8	0.27367550051779915
9	0.28446833715793773
10-11	0.2820271002988588
12-13	0.31260680411258474
14-15	0.3623309443475088
16-17	0.32815362832040335
18-19	0.30965162265159435
20-21	0.29898727216193366
22-23	0.2952611737980763
24-25	0.2817701279979031
26-27	0.28421136485698206
28-29	0.27740159888165655
30-31	0.2767591681292674
32-33	0.27457490357114406
34-35	0.26352509463004986
36-37	0.2663517899405623
38-39	0.25979899626619246
40-41	0.27059183290633104
42-43	0.2640390392319612
44-45	0.25710078710615786
46-47	0.2572292732566357
48-49	0.24823524272318687
50-51	0.25658684250424646
52-53	0.25504500869851243
54-55	0.25710078710615786
56-57	0.25658684250424646
58-59	0.24939161807748744
60-61	0.2457940058641079
62-63	0.247335839669842
64-65	0.2438667136069403
66-67	0.24142547674786136
68-69	0.25016253498035446
70-71	0.24643643661649708
72-73	0.2359005722773142
74-75	0.25286074414038906
76-77	0.2671227068434293
78-79	0.25106193803369936
80-81	0.25620138405281295
82-83	0.24977707652892095
84-85	0.2515758826356107
86-87	0.24707886736888632
88-89	0.2360290584277921
90-91	0.2271635140448211
92-93	0.23191750161250121
94-95	0.22215255417618535
96-97	0.21611370510372685
98-99	0.21701310815707175
100-101	0.2064772438178889
102-103	0.20866150837601216
104-105	0.2147003574484706
106-107	0.21007485603126838
108-109	0.21007485603126838
110-111	0.2104603144827019
112-113	0.20673421611884457
114-115	0.1965838102310952
116-117	0.20223720085212016
118-119	0.19427105952249407
120-121	0.19491349027488328
122-123	0.18874615505194695
124-125	0.18784675199860207
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	389147.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.03011408671323
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.06806724882998	67.06863153486992
2	8.81501382312392	13.580430409423794
3	2.0076042273322816	4.639379480171203
4	0.7734428635086594	2.383135680625048
5	0.36058947861018026	1.3888124337905312
6	0.23127901127511863	1.0689269174630776
7	0.14000346968390706	0.7549138269600941
8	0.10301186309338731	0.6348012453094807
9	0.07328561960413432	0.508067967511974
>10	0.39914385807439995	5.465345037436683
>50	0.021704488017132113	1.1368914479210321
>100	0.006092487863917692	0.8714407872917697
>500	7.615609829897115E-4	0.49922323122537593
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	902	0.23178901546202338	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	800	0.205577840764544	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	462	0.11872120304152414	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.5697230095568E-4	0.0
2	0.0	0.0	0.0	2.5697230095568E-4	0.0
3	0.0	0.0	0.0	2.5697230095568E-4	0.0
4	0.0	0.0	0.0	2.5697230095568E-4	0.0
5	0.0	0.0	0.0	2.5697230095568E-4	0.0
6	0.0	0.0	0.0	2.5697230095568E-4	0.0
7	0.0	0.0	0.0	2.5697230095568E-4	0.0
8	0.0	0.0	0.0	2.5697230095568E-4	0.0
9	0.0	0.0	0.0	2.5697230095568E-4	0.0
10-11	0.0	0.0	0.0	2.5697230095568E-4	0.0
12-13	0.0	0.0	0.0	2.5697230095568E-4	0.0
14-15	0.0	0.0	0.0	3.8545845143352E-4	0.0
16-17	0.0	0.0	0.0	6.424307523892E-4	0.0
18-19	0.0	0.0	0.0	0.0012848615047784	0.0
20-21	0.0	0.0	0.0	0.00282669531051248	0.0
22-23	0.0	0.0	0.0	0.00603884907245848	0.0
24-25	0.0	0.0	0.0	0.009764947436315839	0.0
26-27	0.0	0.0	0.0	0.017088658013552718	0.0
28-29	0.0	0.0	0.0	0.02916635615846968	0.0
30-31	0.0	0.0	0.0	0.051394460191136	0.0
32-33	0.0	0.0	0.0	0.09135365298974424	0.0
34-35	0.0	0.0	0.0	0.13439651339982062	0.0
36-37	0.0	0.0	0.0	0.20300811775498717	0.0
38-39	0.0	0.0	0.0	0.2879374632208394	0.0
40-41	0.0	0.0	0.0	0.3839166176277859	0.0
42-43	0.0	0.0	0.0	0.4744993537146631	0.0
44-45	0.0	0.0	0.0	0.558914754578604	0.0
46-47	0.0	0.0	0.0	0.6583630350484522	0.0
48-49	0.0	0.0	0.0	0.7646210814936258	0.0
50-51	0.0	0.0	0.0	0.8657396819196859	0.0
52-53	0.0	0.0	0.0	0.9609479194237653	0.0
54-55	0.0	0.0	0.0	1.057569504583101	0.0
56-57	0.0	0.0	0.0	1.1625426895234963	0.0
58-59	0.0	0.0	0.0	1.2803644895116755	0.0
60-61	0.0	0.0	0.0	1.3854661606025487	0.0
62-63	0.0	0.0	0.0	1.5000758068287818	0.0
64-65	0.0	0.0	0.0	1.6091605485844682	0.0
66-67	0.0	0.0	0.0	1.725697487067869	0.0
68-69	0.0	0.0	0.0	1.8626637234772465	0.0
70-71	0.0	0.0	0.0	2.000143904488535	0.0
72-73	0.0	0.0	0.0	2.1386519747036465	0.0
74-75	0.0	0.0	0.0	2.263412026817629	0.0
76-77	0.0	0.0	0.0	2.4092438076099776	0.0
78-79	0.0	0.0	0.0	2.571393329513012	0.0
80-81	0.0	0.0	0.0	2.7379113805322923	0.0
82-83	0.0	0.0	0.0	2.909825849871642	0.0
84-85	0.0	0.0	0.0	3.0749305532356663	0.0
86-87	0.0	0.0	0.0	3.2433758965121147	0.0
88-89	0.0	0.0	0.0	3.4238989379334805	0.0
90-91	0.0	0.0	0.0	3.605578354709146	0.0
92-93	0.0	0.0	0.0	3.800620331134507	0.0
94-95	0.0	0.0	0.0	3.9856403878225968	0.0
96-97	0.0	0.0	0.0	4.167319804598263	2.5697230095568E-4
98-99	0.0	0.0	0.0	4.374310993018063	2.5697230095568E-4
100-101	0.0	0.0	0.0	4.597876894849504	2.5697230095568E-4
102-103	0.0	0.0	0.0	4.833520494825863	2.5697230095568E-4
104-105	0.0	0.0	0.0	5.0736611100689455	2.5697230095568E-4
106-107	0.0	0.0	0.0	5.300953110264245	2.5697230095568E-4
108-109	0.0	0.0	0.0	5.545333768473096	2.5697230095568E-4
110-111	0.0	0.0	0.0	5.788815023628603	2.5697230095568E-4
112-113	0.0	0.0	0.0	6.0626190102968796	2.5697230095568E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGGC	75	0.0038910601	31.76038	3
ATAGACT	155	2.090674E-4	23.051888	4
CATGGGG	1515	0.0	22.405218	4
GGTAAAC	135	0.002541569	22.052977	8
GTATCAA	2605	0.0	21.9599	1
TAGACTG	165	3.1915432E-4	21.654804	5
GTACATA	195	3.9838746E-5	21.39096	1
TATCAAC	2900	0.0	19.918686	2
TATTCAC	150	0.004677275	19.850237	2
TATTCTC	210	7.083793E-5	19.850235	5
AACGCAG	2770	0.0	19.768387	6
ATCAACG	2840	0.0	19.291075	3
CAACGCA	2825	0.0	19.182707	5
TCAACGC	2880	0.0	18.816372	4
CCAGTAC	190	8.2606915E-4	18.805487	3
GTCCTAC	160	0.0067660105	18.621595	1
TGTTAAG	160	0.0067912326	18.609596	2
ACATGGG	3725	0.0	18.065048	3
GTACAAG	330	3.6918027E-7	18.057304	1
ACGCAGA	3035	0.0	18.046965	7
>>END_MODULE