FastQCFastQC Report
Sun 1 Jan 2017
ERR1142469_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142469_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389147
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19710.5064924051836452No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16090.41346843223768914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9940.25543046714994594No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5430.13953595941893424No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4370.11229689551763215No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4180.10741442179947425No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTACG602.1976533E-549.5567259
ATCACGC700.002790554433.990493
GGTATCA21750.030.091271
TTTTACG850.007214443827.9921682
GTATCAA28550.026.8838041
TAAACCG900.00953435826.4370465
CGCACTA600.00129152324.77836258-59
GTTTAGA1250.001631805923.7994611
ATCAACG33850.022.4929683
GTATTAG1350.002552607822.0365371
GAGTACT16350.022.00500312-13
ATGGGGG7300.022.0006945
CAACGCA35150.021.9995335
GTATATA1903.298964E-521.9205551
TCAACGC35650.021.857844
AACGCAG37000.021.7005986
TATCAAC35200.021.6303122
CATGGGG11900.020.9941254
TGGGGGG5950.020.9914266
TTCAACG2004.9089816E-520.8191764
Produced by FastQC (version 0.11.5)