##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142469_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	389147
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.14543604344888	33.0	14.0	33.0	14.0	33.0
2	30.24375107607151	33.0	27.0	33.0	27.0	33.0
3	29.769842758649045	33.0	27.0	33.0	27.0	33.0
4	30.837506135213687	33.0	33.0	33.0	27.0	33.0
5	31.711283910707266	33.0	33.0	33.0	27.0	33.0
6	33.83317615194258	37.0	33.0	37.0	27.0	37.0
7	35.459140119286545	37.0	37.0	37.0	33.0	37.0
8	35.207705571416454	37.0	37.0	37.0	33.0	37.0
9	35.80277375901652	37.0	37.0	37.0	33.0	37.0
10-11	35.91544968867806	37.0	37.0	37.0	33.0	37.0
12-13	35.99065648713725	37.0	37.0	37.0	33.0	37.0
14-15	36.07005064924052	37.0	37.0	37.0	37.0	37.0
16-17	36.08848455724957	37.0	37.0	37.0	37.0	37.0
18-19	36.120182090572456	37.0	37.0	37.0	37.0	37.0
20-21	36.1580251164727	37.0	37.0	37.0	37.0	37.0
22-23	36.15014506086389	37.0	37.0	37.0	37.0	37.0
24-25	36.15423092044908	37.0	37.0	37.0	37.0	37.0
26-27	36.11509660873655	37.0	37.0	37.0	37.0	37.0
28-29	36.08959724731271	37.0	37.0	37.0	37.0	37.0
30-31	36.01953760404166	37.0	37.0	37.0	37.0	37.0
32-33	35.977161586752565	37.0	37.0	37.0	37.0	37.0
34-35	35.90856797045846	37.0	37.0	37.0	35.0	37.0
36-37	35.83198508532765	37.0	37.0	37.0	33.0	37.0
38-39	35.779348164061396	37.0	37.0	37.0	33.0	37.0
40-41	35.66205829673619	37.0	37.0	37.0	33.0	37.0
42-43	35.611750829378096	37.0	37.0	37.0	33.0	37.0
44-45	35.53239007367396	37.0	37.0	37.0	33.0	37.0
46-47	35.408448478338514	37.0	37.0	37.0	33.0	37.0
48-49	35.28016919056295	37.0	37.0	37.0	33.0	37.0
50-51	35.19898650124503	37.0	37.0	37.0	33.0	37.0
52-53	35.121085348210315	37.0	37.0	37.0	33.0	37.0
54-55	35.078797986365046	37.0	37.0	37.0	33.0	37.0
56-57	35.03244146813415	37.0	37.0	37.0	33.0	37.0
58-59	34.98489902273434	37.0	37.0	37.0	27.0	37.0
60-61	34.963318488900086	37.0	37.0	37.0	27.0	37.0
62-63	34.95222242494482	37.0	37.0	37.0	27.0	37.0
64-65	34.9186361452099	37.0	37.0	37.0	27.0	37.0
66-67	34.87232074254716	37.0	37.0	37.0	27.0	37.0
68-69	34.838352858945335	37.0	37.0	37.0	27.0	37.0
70-71	34.80016934474633	37.0	37.0	37.0	27.0	37.0
72-73	34.788276666658106	37.0	37.0	37.0	27.0	37.0
74-75	34.67162794522379	37.0	37.0	37.0	27.0	37.0
76-77	34.61855802563042	37.0	37.0	37.0	27.0	37.0
78-79	34.60052499441085	37.0	37.0	37.0	27.0	37.0
80-81	34.58088588631031	37.0	37.0	37.0	27.0	37.0
82-83	34.526312678756355	37.0	37.0	37.0	27.0	37.0
84-85	34.44473553695647	37.0	37.0	37.0	27.0	37.0
86-87	34.26591493702894	37.0	37.0	37.0	27.0	37.0
88-89	34.19301960441684	37.0	37.0	37.0	27.0	37.0
90-91	34.1588320094977	37.0	37.0	37.0	27.0	37.0
92-93	34.11490773409534	37.0	37.0	37.0	27.0	37.0
94-95	34.12830241528266	37.0	37.0	37.0	27.0	37.0
96-97	34.08995957825706	37.0	37.0	37.0	27.0	37.0
98-99	34.03733550560585	37.0	37.0	37.0	27.0	37.0
100-101	33.97815863928027	37.0	37.0	37.0	22.0	37.0
102-103	33.87128000472829	37.0	37.0	37.0	22.0	37.0
104-105	33.795826512860174	37.0	37.0	37.0	22.0	37.0
106-107	33.712018851488	37.0	33.0	37.0	22.0	37.0
108-109	33.62457374719579	37.0	33.0	37.0	22.0	37.0
110-111	33.54448062043392	37.0	33.0	37.0	22.0	37.0
112-113	33.488108606773274	37.0	33.0	37.0	22.0	37.0
114-115	33.37866795838077	37.0	33.0	37.0	18.0	37.0
116-117	33.24208589556132	37.0	33.0	37.0	14.0	37.0
118-119	33.10020120931165	37.0	33.0	37.0	14.0	37.0
120-121	33.01726468403971	37.0	33.0	37.0	14.0	37.0
122-123	32.89144076659977	37.0	33.0	37.0	14.0	37.0
124-125	31.66048691111585	37.0	30.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	4.0
16	2.0
17	9.0
18	48.0
19	236.0
20	983.0
21	2441.0
22	3623.0
23	4743.0
24	4098.0
25	3042.0
26	2985.0
27	3359.0
28	4437.0
29	5868.0
30	7517.0
31	10371.0
32	14382.0
33	21708.0
34	35417.0
35	73815.0
36	190059.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.78726767543059	16.641263362437893	12.647761124976546	25.923707837154968
2	17.424520810901793	20.90341182123979	37.7248186417986	23.94724872605982
3	20.34506329244037	25.517158084626335	28.81848971851182	25.319288904421477
4	14.294598185261611	17.516259922342968	35.81731325180459	32.371828640590834
5	15.5444768525062	34.76068716512265	33.062047875415985	16.63278810695517
6	29.60353192714115	34.774988692899136	19.03267752148349	16.588801858476216
7	25.791387707372238	32.07365810406199	23.33234814119945	18.802606047366325
8	25.786399483999624	31.82319277805045	21.915368742403256	20.475038995546672
9	26.882643319876546	15.84414116002436	21.152931925467755	36.12028359463134
10-11	25.1081210956271	25.303548530503896	27.313457382428748	22.274872991440255
12-13	26.240212567487355	24.13625185341272	27.228656523113376	22.394879055986554
14-15	24.489074822624872	24.272832631370665	26.068426584298475	25.169665961705988
16-17	23.266657586978702	26.548065384032256	26.81364625706995	23.371630771919094
18-19	23.140484187209463	25.337468874230044	29.225973732291394	22.296073206269096
20-21	24.267303961248377	24.405298796078583	28.996774981048194	22.330622261624843
22-23	24.12918511513644	24.27951391119551	28.827024235057703	22.764276738610345
24-25	23.480458541373824	24.77264375672946	28.892680657951882	22.854217043944832
26-27	23.106692329633788	24.899074128799654	28.920305180304616	23.073928361261938
28-29	23.050929340326405	24.790760303946836	29.37270491613709	22.785605439589666
30-31	23.49870357474168	24.516313886526174	29.263620174381405	22.721362364350746
32-33	23.07868234882962	24.86309800666586	29.0577853613159	23.000434283188614
34-35	23.080609641086788	24.80823442041182	29.197064348433884	22.914091590067507
36-37	23.30613278032823	24.84642673132098	28.831686781199366	23.015753707151422
38-39	23.28156198043413	24.819027257051964	28.752502267780557	23.146908494733353
40-41	22.975287528380218	24.704890712875077	29.066353493834413	23.253468264910296
42-43	23.302081098201057	24.728796812503294	28.768031494646607	23.201090594649045
44-45	23.30597434902492	24.88930918136334	28.52752301829386	23.27719345131788
46-47	23.250258911569173	24.853968735303717	28.401121478995396	23.494650874131715
48-49	23.40016807197798	25.040154811253114	28.189705462853265	23.36997165391564
50-51	23.199459330278792	25.349546572374965	27.923381138747054	23.527612958599192
52-53	23.129048919816935	25.218619185038044	27.721914854797802	23.930417040347223
54-55	22.818497894111996	25.287616247844646	27.785900957735766	24.107984900307596
56-57	23.321649659383215	25.07805533641529	27.40378314621467	24.196511857986827
58-59	23.219503169753335	25.039124032820503	27.8326699165097	23.908702880916465
60-61	23.26720142671205	25.245762200096877	27.919562427003715	23.567473946187363
62-63	22.81618514340339	25.249969805754635	28.005997733504305	23.927847317337665
64-65	22.772885310692363	25.015611067283057	28.053023664579197	24.158479957445387
66-67	23.072208266140688	25.028815203633865	27.980751315476688	23.91822521474876
68-69	23.08639151785829	24.919503426725633	27.98274174026782	24.01136331514826
70-71	22.885696150811903	25.052615078620676	28.139623330001257	23.92206544056616
72-73	23.255093833435694	25.14358327315899	28.136925120841223	23.464397772564094
74-75	23.206790663223842	25.035619862767085	28.29130103799109	23.466288436017983
76-77	22.92383829294942	24.883604344752232	28.22533581849733	23.967221543801013
78-79	23.009230468770074	25.148273398672995	28.13005915517569	23.71243697738124
80-81	22.802951069904175	25.222345283401904	28.29611946128327	23.678584185410653
82-83	23.02818729169183	25.006745522900086	28.312951146995864	23.652116038412217
84-85	22.88861374363405	25.262963005820477	27.967615146360565	23.880808104184908
86-87	22.802929899060935	25.415985646740314	27.868043775896467	23.913040678302284
88-89	22.948796244044352	24.858339968614477	27.960301692705315	24.23256209463586
90-91	23.105444895322155	24.980469830875816	27.91816762612242	23.99591764767961
92-93	23.07858557036835	25.032708074166603	27.701069428596053	24.18763692686899
94-95	22.64378582359086	25.12588446767477	27.94847184800583	24.281857860728543
96-97	22.797683137734584	24.971797289970112	27.717032381079644	24.513487191215656
98-99	22.738065563912865	25.121355169126318	27.499505328320662	24.641073938640154
100-101	22.95739142277854	25.424967942705457	27.16492739247636	24.45271324203964
102-103	22.91614736847515	25.325391176085134	27.42395547183969	24.334505983600028
104-105	23.028976866818454	25.480035360206404	27.17429292436102	24.31669484861412
106-107	22.993592189846957	25.241786580218406	27.335921127527886	24.42870010240675
108-109	23.060159843754015	25.411302135533116	27.08169506334644	24.44684295736643
110-111	22.76949248798965	25.771983856854863	27.036812898069734	24.421710757085755
112-113	22.746288677543447	25.835609679632633	26.91373696829219	24.504364674531733
114-115	22.82636487800867	25.733624740297035	26.861092159379563	24.578918222314734
116-117	22.833945570627	25.679660487759755	26.814451626829484	24.671942314783767
118-119	22.647868286277422	25.481501848915705	27.125225171978713	24.74540469282816
120-121	22.574805213494233	25.597336718541207	27.359152396028207	24.468705671936352
122-123	22.56139710700584	25.86374814658728	27.214394560410334	24.36046018599655
124-125	22.529918000138764	25.601507913462008	27.142827774594174	24.72574631180505
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	121.0
1	113.5
2	198.5
3	304.5
4	242.0
5	194.5
6	279.0
7	530.0
8	880.5
9	1016.5
10	898.5
11	745.0
12	688.0
13	659.5
14	646.0
15	630.5
16	628.5
17	639.0
18	611.0
19	616.5
20	656.0
21	674.0
22	667.0
23	698.0
24	785.5
25	861.0
26	974.5
27	1178.0
28	1406.0
29	1824.5
30	2481.5
31	3113.5
32	3851.0
33	4751.5
34	5552.0
35	6333.0
36	7241.0
37	8250.0
38	8812.0
39	9170.0
40	9355.0
41	9460.5
42	9901.5
43	10666.5
44	11779.5
45	12886.0
46	13963.5
47	14918.5
48	15994.0
49	16801.5
50	17478.5
51	17073.5
52	15975.5
53	15779.5
54	15409.0
55	14575.5
56	13683.5
57	12262.0
58	10738.5
59	9596.0
60	8315.5
61	6853.5
62	5523.0
63	4361.5
64	3283.5
65	2538.5
66	1939.5
67	1499.5
68	1214.0
69	985.5
70	859.0
71	730.5
72	623.0
73	572.0
74	436.5
75	364.0
76	300.5
77	197.5
78	142.5
79	84.0
80	63.0
81	46.0
82	26.5
83	14.0
84	8.5
85	4.0
86	0.5
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0231275070860112
2	0.0
3	2.5697230095568E-4
4	0.0
5	0.0030836676114681595
6	0.00282669531051248
7	0.01336255964969536
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.1394460191136E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	1.2848615047784E-4
38-39	0.0
40-41	0.00295518146099032
42-43	3.8545845143351994E-4
44-45	0.0
46-47	0.0046255014172022395
48-49	0.00719522442675904
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	1.2848615047784E-4
62-63	0.0
64-65	0.0
66-67	0.00758068287819256
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.08197416400486192
76-77	0.044199235764376954
78-79	2.5697230095568E-4
80-81	0.0
82-83	0.0
84-85	0.06719825669991032
86-87	0.24219639365072837
88-89	0.27496036202257756
90-91	0.16587562026689143
92-93	0.12373216291015991
94-95	1.2848615047784E-4
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0023127507086011198
106-107	0.003212153761946
108-109	0.00436852911624656
110-111	8.9940305334488E-4
112-113	0.0
114-115	1.2848615047784E-4
116-117	1.2848615047784E-4
118-119	0.0
120-121	7.709169028670399E-4
122-123	0.0
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	389147.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.61580001390223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.90713876886713	63.241227473754705
2	9.537382294587749	14.042040553090073
3	2.163259230660909	4.777501767076845
4	0.8356335400549126	2.4606332627836642
5	0.42287431923475627	1.5565115657900432
6	0.2575225377109165	1.1374636581119655
7	0.17507976109600137	0.9022045675527514
8	0.10571811875485157	0.6226019110482496
9	0.08200995502978493	0.5433505603759586
>10	0.47046106925737985	6.161104383646735
>50	0.030713684130238398	1.5198061747649163
>100	0.010631659890834505	1.552271994206672
>500	7.875303622840374E-4	0.4455353977769663
>1k	7.875303622840374E-4	1.0377467300205203
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1971	0.5064924051836452	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1609	0.41346843223768914	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	994	0.25543046714994594	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	543	0.13953595941893424	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	437	0.11229689551763215	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	418	0.10741442179947425	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5697230095568E-4
2	0.0	0.0	0.0	0.0	2.5697230095568E-4
3	0.0	0.0	0.0	0.0	2.5697230095568E-4
4	0.0	0.0	0.0	0.0	2.5697230095568E-4
5	0.0	0.0	0.0	0.0	2.5697230095568E-4
6	0.0	0.0	0.0	0.0	2.5697230095568E-4
7	0.0	0.0	0.0	0.0	2.5697230095568E-4
8	0.0	0.0	0.0	0.0	2.5697230095568E-4
9	0.0	0.0	0.0	0.0	2.5697230095568E-4
10-11	0.0	0.0	0.0	0.0	2.5697230095568E-4
12-13	0.0	0.0	0.0	0.0	5.1394460191136E-4
14-15	0.0	0.0	0.0	1.2848615047784E-4	7.7091690286704E-4
16-17	0.0	0.0	0.0	3.8545845143352E-4	7.7091690286704E-4
18-19	0.0	0.0	0.0	0.0012848615047784	7.7091690286704E-4
20-21	0.0	0.0	0.0	0.00282669531051248	7.7091690286704E-4
22-23	0.0	0.0	0.0	0.00616733522293632	7.7091690286704E-4
24-25	0.0	0.0	0.0	0.010021919737271519	7.7091690286704E-4
26-27	0.0	0.0	0.0	0.0173456303145084	0.00102788920382272
28-29	0.0	0.0	0.0	0.029423328459425357	0.00102788920382272
30-31	0.0	0.0	0.0	0.0520368909435252	0.00102788920382272
32-33	0.0	0.0	0.0	0.09212456989261128	0.00102788920382272
34-35	0.0	0.0	0.0	0.13478197185125415	0.00102788920382272
36-37	0.0	0.0	0.0	0.203136603905465	0.00102788920382272
38-39	0.0	0.0	0.0	0.2888368662741843	0.00102788920382272
40-41	0.0	0.0	0.0	0.38532996528304214	0.00102788920382272
42-43	0.0	0.0	0.0	0.47655513212230854	0.00102788920382272
44-45	0.0	0.0	0.0	0.5622553944910278	0.00102788920382272
46-47	0.0	0.0	0.0	0.6623461057132651	0.00102788920382272
48-49	0.0	0.0	0.0	0.7691180967603501	0.00102788920382272
50-51	0.0	0.0	0.0	0.8725494478950114	0.00102788920382272
52-53	0.0	0.0	0.0	0.9681431438505244	0.00102788920382272
54-55	0.0	0.0	0.0	1.0651501874612936	0.00102788920382272
56-57	0.0	0.0	0.0	1.1701233724016888	0.00102788920382272
58-59	0.0	0.0	0.0	1.288844575443213	0.00102788920382272
60-61	0.0	0.0	0.0	1.3934323019321746	0.00102788920382272
62-63	0.0	0.0	0.0	1.5073995174060189	0.0012848615047783998
64-65	0.0	0.0	0.0	1.6153278838074043	0.0012848615047783998
66-67	0.0	0.0	0.0	1.7309654192374604	0.0012848615047783998
68-69	0.0	0.0	0.0	1.8697304617535275	0.0012848615047783998
70-71	0.0	0.0	0.0	2.0063112397114713	0.0012848615047783998
72-73	0.0	0.0	0.0	2.1452047683780164	0.0012848615047783998
74-75	0.0	0.0	0.0	2.2689369312881764	0.0012848615047783998
76-77	0.0	0.0	0.0	2.4132268782747905	0.0012848615047783998
78-79	0.0	0.0	0.0	2.575247914027347	0.0012848615047783998
80-81	0.0	0.0	0.0	2.7433077988523618	0.0012848615047783998
82-83	0.0	0.0	0.0	2.9159931850945786	0.0012848615047783998
84-85	0.0	0.0	0.0	3.0816118330605144	0.0012848615047783998
86-87	0.0	0.0	0.0	3.2499286901864846	0.0012848615047783998
88-89	0.0	0.0	0.0	3.432635996165973	0.0012848615047783998
90-91	0.0	0.0	0.0	3.6153433021454617	0.0012848615047783998
92-93	0.0	0.0	0.0	3.810256792420345	0.0012848615047783998
94-95	0.0	0.0	0.0	3.9984890028703806	0.0012848615047783998
96-97	0.0	0.0	0.0	4.1804253919470025	0.0012848615047783998
98-99	0.0	0.0	0.0	4.389472358774448	0.0012848615047783998
100-101	0.0	0.0	0.0	4.615864955916402	0.0012848615047783998
102-103	0.0	0.0	0.0	4.855877085009007	0.0012848615047783998
104-105	0.0	0.0	0.0	5.096917103305435	0.0012848615047783998
106-107	0.0	0.0	0.0	5.324594561952167	0.0012848615047783998
108-109	0.0	0.0	0.0	5.570774026267709	0.00141334765525624
110-111	0.0	0.0	0.0	5.8170819767337285	0.00154183380573408
112-113	0.0	0.0	0.0	6.094098117163951	0.00154183380573408
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTACG	60	2.1976533E-5	49.556725	9
ATCACGC	70	0.0027905544	33.99049	3
GGTATCA	2175	0.0	30.09127	1
TTTTACG	85	0.0072144438	27.992168	2
GTATCAA	2855	0.0	26.883804	1
TAAACCG	90	0.009534358	26.437046	5
CGCACTA	60	0.001291523	24.778362	58-59
GTTTAGA	125	0.0016318059	23.799461	1
ATCAACG	3385	0.0	22.492968	3
GTATTAG	135	0.0025526078	22.036537	1
GAGTACT	1635	0.0	22.005003	12-13
ATGGGGG	730	0.0	22.000694	5
CAACGCA	3515	0.0	21.999533	5
GTATATA	190	3.298964E-5	21.920555	1
TCAACGC	3565	0.0	21.85784	4
AACGCAG	3700	0.0	21.700598	6
TATCAAC	3520	0.0	21.630312	2
CATGGGG	1190	0.0	20.994125	4
TGGGGGG	595	0.0	20.991426	6
TTCAACG	200	4.9089816E-5	20.819176	4
>>END_MODULE