ERR1142462_2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1142462_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	76181
Sequences flagged as poor quality	0
Sequence length	125
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	473	0.6208897231593179	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	370	0.4856854071225109	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	301	0.39511164201047505	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	215	0.2822226014360536	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	200	0.2625326524986545	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	189	0.24809335661122853	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	183	0.24021737703626891	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	180	0.23627938724878908	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	165	0.21658943831139	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	147	0.19296149958651107	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	145	0.19033617306152453	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	142	0.18639818327404473	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	139	0.1824601934865649	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	132	0.17327155064911198	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	130	0.17064622412412545	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	128	0.16802089759913888	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	123	0.16145758128667254	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	116	0.15226893844921963	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	112	0.14701828539924652	No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC	107	0.14045496908678018	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	105	0.13782964256179364	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	102	0.1338916527743138	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	102	0.1338916527743138	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	101	0.13257898951182054	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	101	0.13257898951182054	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	96	0.12601567319935417	No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	93	0.12207768341187435	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	93	0.12207768341187435	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	92	0.12076502014938108	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	91	0.11945235688688781	No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT	90	0.11813969362439454	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	90	0.11813969362439454	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	85	0.11157637731192818	No Hit
TATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAA	79	0.10370039773696853	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	79	0.10370039773696853	No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA	78	0.10238773447447526	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	77	0.10107507121198199	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACAAGA	15	2.686114E-4	119.06399	2
GTACAAA	45	4.2327883E-8	79.375984	1
GTACATA	25	0.0020450559	71.438385	1
GTACAAG	50	8.785173E-8	71.438385	1
ATGGGGT	30	0.004212347	59.531994	5
TACAAGG	30	0.004212347	59.531994	2
CTGTAGA	15	0.0042315177	59.492752	112-113
ACATTCC	15	0.0042315177	59.492752	106-107
CCTGTAG	15	0.0042315177	59.492752	110-111
CATTCCT	15	0.0042315177	59.492752	106-107
CATACCC	15	0.0042315177	59.492752	106-107
ATAGTAA	15	0.0042315177	59.492752	116-117
GCATACC	15	0.0042315177	59.492752	106-107
TACAAAA	90	5.073214E-6	39.687992	2
GGTAAAA	65	0.0019224321	36.63507	1
AATTCAC	65	0.0019224321	36.63507	5
CATACTG	35	0.0027830617	33.995857	64-65
GAGTACT	90	5.857146E-10	33.095158	12-13
CTTCATA	90	2.3645973E-4	33.073326	1
GAACATG	65	1.7157436E-6	32.055687	14-15

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content