FastQCFastQC Report
Sun 1 Jan 2017
ERR1142461_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142461_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435034
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12560.288713066105178No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10110.23239562884740045No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9770.22458014775856597No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7190.16527443831976352No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG5200.11953088724099725No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG4970.11424394415149161No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA3900.027.500371
GTACAAC1350.002531878422.0681971
CATGGGG17500.021.10254
GTATACG1754.731498E-420.4288441
CATGGGT4700.020.276944
GTAAAAA4254.0017767E-1119.6277141
ATGGGGG11350.019.417185
GCTTAAA1908.2317105E-418.816041
GTACATA2601.7434002E-518.3335781
CTTATAG1959.824153E-418.3272343
GTATAAA5453.6379788E-1217.492591
GTATCAA43350.016.7687761
TATACAG2502.6895286E-416.6777845
TGGGGGG7150.016.6476766
GTACTGG2553.1196754E-416.3564281
GTCCTAG2200.00219861416.250221
ATGGGGC4450.016.0620695
GTGTAAG2603.6170456E-416.0418821
ACGTTTA1354.8522782E-415.43704926-27
GTACATG56750.015.4341271
Produced by FastQC (version 0.11.5)