FastQCFastQC Report
Sun 1 Jan 2017
ERR1142461_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142461_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435034
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24310.5588068978516622No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19330.44433308660932247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12340.28365599010652043No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11210.2576810088406883No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9630.221362008486693No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG6470.14872400777870234No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5660.13010477342000856No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5440.12504769742135097No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG5010.11516341251488389No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT4400.10114151997315153No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTAT2401.3020326E-824.783026
GCTATTG2901.4733814E-1024.6064458
GTCTAAC1250.001631935823.7999081
CTATTGA3052.764864E-1023.3962929
CATGGGG15500.022.6430914
GTATCAA44850.022.5528661
ATTTAGG2653.712921E-822.4527441
GTATTAG1653.2109168E-421.6362781
GGCTATT2806.6733264E-821.2425887
GTATTAT1450.003863653420.5171621
GGTATCA36200.020.3811361
GAGTACT20750.020.20396612-13
AGTACTT21600.020.09716212-13
TATCAAC51150.019.8868432
ATCAACG50400.019.8286953
GTACTGC1805.789291E-419.8264146
CAACGCA51800.019.6372995
TCAACGC51900.019.5994644
ATAAACG1856.9567375E-419.2927843
AACGCAG53400.019.2694936
Produced by FastQC (version 0.11.5)