FastQCFastQC Report
Sun 1 Jan 2017
ERR1142460_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142460_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93359
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4160.4455917479835902No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3700.3963195835430971No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2560.2742103064514401No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2080.2227958739917951No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1870.20030205979070043No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1710.1831639156374854No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1660.1778082455896057No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1530.16388350346511854No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1460.15638556539808696No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1310.1403185552544479No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1290.13817628723529599No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1290.13817628723529599No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1270.13603401921614414No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA1120.11996700907250507No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA1070.11461133902462538No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC990.10604226694801787No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC990.10604226694801787No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC950.10175773090971411No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC940.10068659690013818No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGG250.002045655271.446081
GTACATA300.00421357259.53841
TAGTACG300.00421357259.53844
TCTCGGG150.004229176359.5064442-43
TTATGTA350.00775409951.0329132
TGAACCG350.00775409951.0329135
GAACAAA558.461072E-443.3006551
GTAAAAA1401.8408173E-938.2746851
GTACTAG801.1845234E-437.21151
AGTACAA650.001923671136.6390152
GTGATCC851.6920324E-435.022593
TCAGTAA350.002774229534.02194276-77
AGTCCTA350.002781545334.0036828-29
GTCCTAC350.002781545334.0036830-31
GCCTCCG350.002781545334.0036850-51
CGATAGC350.002781545334.0036862-63
CCAATGC452.3838249E-433.05913518-19
TAGGCAT1152.7249735E-531.063515
ATCGTCA400.005333961529.769210-11
CTAGTAC800.005320428429.76923
Produced by FastQC (version 0.11.5)