FastQCFastQC Report
Sun 1 Jan 2017
ERR1142459_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142459_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503188
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10900.21661883828708156No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8390.16673688561730407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24100.026.454931
CTAACGC1159.985544E-425.893783
GGTATCA18200.025.53661
GTATGAC1552.0836218E-423.0653171
CATGGGT6800.022.7712964
TATCAAC27950.022.5864162
CATGGGG17950.022.2297024
ATCAACG27300.021.8152713
AACGCAG28000.021.2529376
GTACATG39800.021.1094021
CAACGCA27800.020.9944535
TCAACGC28100.020.9822544
ACATGGG38600.020.6747743
TACATGG40750.020.0224042
ATGGGGG10600.019.6646165
ACGCAGA29950.019.4756857
GTAAGGC1856.904221E-419.3153613
CGCAGAG30500.019.1302058
GTATAGG1600.00677113518.6204381
TTTTGCG800.00685021718.58520786-87
Produced by FastQC (version 0.11.5)