##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142459_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	503188
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.82240633719405	33.0	33.0	33.0	27.0	33.0
2	30.9327070597868	33.0	33.0	33.0	27.0	33.0
3	31.03232787745336	33.0	33.0	33.0	27.0	33.0
4	31.032133119231776	33.0	33.0	33.0	27.0	33.0
5	31.04215521832794	33.0	33.0	33.0	27.0	33.0
6	34.24273035128024	37.0	37.0	37.0	27.0	37.0
7	34.215841792729556	37.0	37.0	37.0	27.0	37.0
8	34.25646279323036	37.0	37.0	37.0	27.0	37.0
9	34.2333660580141	37.0	37.0	37.0	27.0	37.0
10-11	34.304846299991254	37.0	37.0	37.0	27.0	37.0
12-13	34.23453162635039	37.0	37.0	37.0	27.0	37.0
14-15	34.21713256277972	37.0	37.0	37.0	27.0	37.0
16-17	34.178274322917076	37.0	37.0	37.0	27.0	37.0
18-19	34.219667400653435	37.0	37.0	37.0	27.0	37.0
20-21	34.193984157014874	37.0	37.0	37.0	27.0	37.0
22-23	34.264770821243744	37.0	37.0	37.0	27.0	37.0
24-25	34.32637105813334	37.0	37.0	37.0	27.0	37.0
26-27	34.36279780121943	37.0	37.0	37.0	27.0	37.0
28-29	34.39264946699842	37.0	37.0	37.0	27.0	37.0
30-31	34.33220486180116	37.0	37.0	37.0	27.0	37.0
32-33	34.33868653465504	37.0	37.0	37.0	27.0	37.0
34-35	34.386403292606346	37.0	37.0	37.0	27.0	37.0
36-37	34.38091429048387	37.0	37.0	37.0	27.0	37.0
38-39	34.344194416401024	37.0	37.0	37.0	27.0	37.0
40-41	34.30877127435471	37.0	37.0	37.0	27.0	37.0
42-43	34.315049246007455	37.0	37.0	37.0	27.0	37.0
44-45	34.29589735844257	37.0	37.0	37.0	27.0	37.0
46-47	34.28277303910268	37.0	37.0	37.0	27.0	37.0
48-49	34.27988346303966	37.0	37.0	37.0	27.0	37.0
50-51	34.19495794812277	37.0	37.0	37.0	27.0	37.0
52-53	34.20766492841642	37.0	37.0	37.0	27.0	37.0
54-55	34.16938301390336	37.0	37.0	37.0	27.0	37.0
56-57	34.14332515878757	37.0	37.0	37.0	27.0	37.0
58-59	34.09220311295182	37.0	37.0	37.0	27.0	37.0
60-61	34.07353116529011	37.0	37.0	37.0	27.0	37.0
62-63	34.05524774040717	37.0	37.0	37.0	27.0	37.0
64-65	34.00349968600205	37.0	37.0	37.0	27.0	37.0
66-67	33.95984602176522	37.0	37.0	37.0	27.0	37.0
68-69	33.92990890084819	37.0	37.0	37.0	27.0	37.0
70-71	33.878271143191014	37.0	37.0	37.0	27.0	37.0
72-73	33.8136382425654	37.0	37.0	37.0	27.0	37.0
74-75	33.72434656629331	37.0	35.0	37.0	22.0	37.0
76-77	33.676956723928235	37.0	33.0	37.0	22.0	37.0
78-79	33.61432705072458	37.0	33.0	37.0	22.0	37.0
80-81	33.555193088865394	37.0	33.0	37.0	22.0	37.0
82-83	33.500473977916805	37.0	33.0	37.0	22.0	37.0
84-85	33.43119569624077	37.0	33.0	37.0	14.0	37.0
86-87	33.336459732744025	37.0	33.0	37.0	14.0	37.0
88-89	33.27151680882692	37.0	33.0	37.0	14.0	37.0
90-91	33.170179932748795	37.0	33.0	37.0	14.0	37.0
92-93	33.084932470567665	37.0	33.0	37.0	14.0	37.0
94-95	33.012925586460725	37.0	33.0	37.0	14.0	37.0
96-97	32.906380915284146	37.0	33.0	37.0	14.0	37.0
98-99	32.7810361137388	37.0	33.0	37.0	14.0	37.0
100-101	32.6887823239028	37.0	33.0	37.0	14.0	37.0
102-103	32.56509296724087	37.0	33.0	37.0	14.0	37.0
104-105	32.40505636064454	37.0	33.0	37.0	14.0	37.0
106-107	32.32233280602876	37.0	33.0	37.0	14.0	37.0
108-109	32.20209444581349	37.0	33.0	37.0	14.0	37.0
110-111	32.11676053482992	37.0	33.0	37.0	14.0	37.0
112-113	31.94969176530442	37.0	33.0	37.0	14.0	37.0
114-115	31.832500973791106	37.0	27.0	37.0	14.0	37.0
116-117	31.64273194114327	37.0	27.0	37.0	14.0	37.0
118-119	31.544858979148948	37.0	27.0	37.0	14.0	37.0
120-121	31.444608178255443	37.0	27.0	37.0	14.0	37.0
122-123	31.350634355350287	37.0	27.0	37.0	14.0	37.0
124-125	29.779832786155474	35.0	24.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	962.0
1	63.0
2	30.0
3	25.0
4	26.0
5	20.0
6	20.0
7	23.0
8	21.0
9	25.0
10	14.0
11	15.0
12	22.0
13	18.0
14	39.0
15	134.0
16	249.0
17	622.0
18	1628.0
19	3130.0
20	4584.0
21	5655.0
22	6050.0
23	6626.0
24	7416.0
25	7942.0
26	9183.0
27	10467.0
28	11784.0
29	13805.0
30	16113.0
31	19314.0
32	23714.0
33	30932.0
34	44150.0
35	77612.0
36	200755.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.83179528108944	19.188706539631863	12.119718661146306	24.859779518132388
2	17.565411296338404	22.544608130587235	35.55785593757854	24.332124635495823
3	20.13605387909114	26.4044641307968	27.903210688067265	25.5562713020448
4	14.775700338204325	18.83475049454406	33.64474985642269	32.744799310828924
5	15.947790115306137	36.68574141187687	31.05825941786457	16.308209054952417
6	30.48259339134872	35.20966462430189	18.043834436901292	16.263907547448106
7	26.850978930521148	32.4682443031087	21.341143783098573	19.339632983271578
8	25.708750944170855	33.411989325637805	20.378943844006034	20.500315886185312
9	26.27455123119915	17.928627522891894	19.770941547440135	36.02587969846883
10-11	25.341374987293737	26.704881796855222	25.621411102983533	22.33233211286751
12-13	26.122555998197527	25.40077043630143	25.114050094310652	23.36262347119039
14-15	24.618446054790557	25.73271025240762	23.266686283408724	26.38215740939309
16-17	24.36720567398516	27.557476221291804	23.989398669648708	24.08591943507433
18-19	24.455707408400635	26.75031477702422	25.306577369351334	23.487400445223813
20-21	26.078175915247126	25.624527137686542	24.701280603349886	23.59601634371645
22-23	25.68456557795356	25.563759887726462	24.56302628221485	24.18864825210513
24-25	24.81889311778899	26.143571988802215	24.666612850341785	24.37092204306701
26-27	24.629725417265895	26.15716724620955	24.73018616133318	24.48292117519138
28-29	24.446650104287183	26.058841291148095	25.093599367799424	24.400909236765294
30-31	24.726116338145793	25.97492418557418	24.977053865863027	24.321905610416998
32-33	24.40983173639865	26.020418840341407	24.85916580716204	24.710583616097896
34-35	24.703869622922006	26.107101150643874	24.85392761346018	24.33510161297394
36-37	24.64873428757903	26.16609455103454	24.651623928217344	24.533547233169088
38-39	24.49372240693709	26.05303248446419	24.837069533273088	24.616175575325634
40-41	24.411328012117224	26.097276613554154	25.06681414606439	24.424581228264227
42-43	24.60557387829934	26.06799453971164	24.98560197686352	24.340829605125496
44-45	24.37949295325585	26.27406828043501	24.798760530261895	24.547678236047247
46-47	24.473533186140493	26.019755457958375	24.872567930558986	24.634143425342145
48-49	24.459104979013762	26.267318752721035	24.92983831679706	24.34373795146814
50-51	24.18401912922188	26.49367340838896	24.87825047324898	24.44405698914018
52-53	24.239290386280377	26.150118411758083	24.883506143761938	24.727085058199602
54-55	24.299041210129715	26.04893484951592	25.043914230003956	24.608109710350405
56-57	24.339061335586276	25.93003654391048	24.811302708712756	24.91959941179049
58-59	24.35666388720361	26.035499421692364	24.95908012723764	24.648756563866385
60-61	24.384869263237803	26.108799365617628	24.968146394092113	24.538184977052452
62-63	23.981773334236564	26.25243573446609	24.912333483429038	24.85345744786831
64-65	24.088809904248834	25.878814805369714	25.131593103365834	24.900782187015615
66-67	24.311215595644857	25.8915995098967	24.905814497893154	24.891370396565293
68-69	24.329884377285108	25.81646214018582	24.853304801241705	25.00034868128737
70-71	24.022717016543655	25.76444792888032	25.084850108932894	25.127984945643135
72-73	24.31774218932094	25.72698133166525	25.098959505790152	24.85631697322366
74-75	24.28757018693935	25.690711214368545	25.225554621279443	24.796163977412657
76-77	24.25637750145232	25.615728695742284	25.097278009340684	25.03061579346471
78-79	24.30365017026423	25.554180689306133	25.23168488862587	24.910484251803762
80-81	24.06812928423402	25.79447632711621	25.267814442850312	24.869579945799458
82-83	24.28530581787714	25.636935853613096	25.25765852288068	24.820099805629088
84-85	24.106716772553877	25.753257065318643	25.087647112153434	25.05237904997405
86-87	24.00608863789639	25.927615738411713	25.15617575363331	24.910119870058587
88-89	24.301716598835412	25.450066835198943	25.11429715467319	25.13391941129246
90-91	24.41290332861578	25.606468197332603	25.02850950798316	24.952118966068458
92-93	24.11180380348874	25.914840339331835	25.089519339663518	24.88383651751591
94-95	23.785042447324283	25.8303222490907	25.336829776469322	25.047805527115692
96-97	23.927273596395747	25.70300961910955	25.2264857635386	25.143231020956097
98-99	23.833307273485982	25.95730102310629	25.173414175295466	25.03597752811227
100-101	24.06770108281105	26.086445643363426	24.918993306367614	24.92685996745791
102-103	24.145780886523692	26.033467703387398	25.17944775501099	24.641303655077916
104-105	23.956048990258118	26.28535690028463	25.048749250586088	24.70984485887117
106-107	23.946819146859777	26.252942652629567	25.195878095511237	24.604360104999422
108-109	23.924785255791072	26.29996674028946	24.983121194800333	24.792126809119136
110-111	23.814962935227268	26.50021709082363	24.89902289212777	24.785797081821332
112-113	23.851783105679868	26.557335911757356	24.810924976424747	24.77995600613803
114-115	23.71534654204714	26.600035645405875	24.93575363647859	24.74886417606839
116-117	23.866495793390712	26.703430310887793	24.785885628422317	24.644188267299178
118-119	23.64310868571736	26.456007877551468	24.996191653897508	24.90469178283367
120-121	23.651474178478548	26.45635611277276	25.09090402207982	24.80126568666887
122-123	23.6560531495573	26.59210223703245	24.99912884267496	24.752715770735286
124-125	23.783808539574633	26.594225840944794	24.859369617465447	24.762596002015123
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1495.0
1	848.0
2	174.0
3	141.5
4	116.5
5	98.0
6	85.0
7	72.0
8	79.5
9	77.0
10	72.0
11	72.0
12	67.0
13	71.0
14	81.0
15	93.5
16	110.5
17	129.5
18	145.0
19	182.0
20	223.5
21	287.0
22	378.5
23	506.0
24	644.5
25	833.5
26	1122.0
27	1411.0
28	1732.5
29	2316.5
30	3094.5
31	3772.5
32	4531.0
33	5272.0
34	6014.0
35	7004.0
36	7945.0
37	8882.0
38	9862.0
39	10566.5
40	11117.5
41	11597.5
42	12186.5
43	13385.0
44	14884.5
45	16136.0
46	17600.0
47	18963.5
48	20219.5
49	21446.0
50	22060.5
51	21796.5
52	21231.5
53	21334.0
54	21212.5
55	20385.5
56	19144.0
57	17592.5
58	16431.0
59	15011.0
60	13005.0
61	11103.0
62	9202.5
63	7175.5
64	5604.0
65	4511.0
66	3478.5
67	2733.5
68	2226.5
69	1839.0
70	1561.0
71	1366.5
72	1205.5
73	1114.0
74	913.0
75	734.5
76	588.0
77	392.0
78	276.0
79	196.0
80	131.0
81	95.5
82	62.0
83	30.0
84	12.5
85	10.0
86	9.5
87	5.5
88	1.5
89	0.5
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	1.5
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4000492857540323
2	0.3620913058340024
3	0.351558463238392
4	0.34102562064278164
5	0.3630849702298147
6	0.29452212691876595
7	0.291541133731329
8	0.28319435280650573
9	0.29452212691876595
10-11	0.291541133731329
12-13	0.3287041821347091
14-15	0.3806728300356924
16-17	0.3460933090614244
18-19	0.32641875402434084
20-21	0.31568717854956796
22-23	0.309228359976788
24-25	0.2944227604791847
26-27	0.29799995230410903
28-29	0.28786457546682354
30-31	0.2920379659292352
32-33	0.28726837682933615
34-35	0.2746488390025199
36-37	0.27733173287121315
38-39	0.2709722807380144
40-41	0.2827968870481808
42-43	0.2748475718816824
44-45	0.270376082100527
46-47	0.26868685262764613
48-49	0.2606381710215665
50-51	0.2659045923193717
52-53	0.2658052258797905
54-55	0.26977988346303966
56-57	0.2635197977694222
58-59	0.2568622463174798
60-61	0.25487491752585517
62-63	0.25557048260292375
64-65	0.2508008935030247
66-67	0.2490122975905626
68-69	0.2577565442737108
70-71	0.253086321613393
72-73	0.2414604481823891
74-75	0.26113500321947264
76-77	0.2791203287836753
78-79	0.26252613337360986
80-81	0.2678919211109963
82-83	0.260836903900729
84-85	0.2618305682965413
86-87	0.2522913900967432
88-89	0.2398705851490894
90-91	0.23102697202635994
92-93	0.23877755431369585
94-95	0.22933774255347902
96-97	0.22148779382656186
98-99	0.22625738292646086
100-101	0.21244544782466993
102-103	0.21701630404540648
104-105	0.22436942057441753
106-107	0.21661883828708156
108-109	0.21443277661629453
110-111	0.21741376980373142
112-113	0.2131410129017385
114-115	0.20260817030612813
116-117	0.21095495123095148
118-119	0.19903097848120382
120-121	0.20072020795408474
122-123	0.1952550537771171
124-125	0.19575188597502324
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	503188.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.00433999535039
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.06912619065889	75.66197499604228
2	7.388806200373406	12.413835764318414
3	1.354475447669874	3.4134544806424354
4	0.478958462967646	1.609383582671383
5	0.22895926210892512	0.9616785849641346
6	0.11492620976630602	0.5792580239751438
7	0.0719349614147966	0.422999426936869
8	0.05082289887759992	0.3415475261490573
9	0.04295789339736323	0.3247784534192506
>10	0.18505774890734575	2.7941856161672494
>50	0.010212298537751885	0.5745520854187588
>100	0.0032249359886399437	0.4668658538459097
>500	2.687446657199815E-4	0.18941027629434537
>1k	2.687446657199815E-4	0.24607532915475144
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	1090	0.21661883828708156	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	839	0.16673688561730407	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	2.9809931874369026E-4	0.0
18-19	0.0	0.0	0.0	6.955650770686106E-4	0.0
20-21	0.0	0.0	0.0	0.0014904965937184511	1.9873287916246017E-4
22-23	0.0	0.0	0.0	0.0029809931874369023	3.9746575832492033E-4
24-25	0.0	0.0	0.0	0.006558185012361184	3.9746575832492033E-4
26-27	0.0	0.0	0.0	0.010830941914354079	3.9746575832492033E-4
28-29	0.0	0.0	0.0	0.020171387234989707	3.9746575832492033E-4
30-31	0.0	0.0	0.0	0.04183327106369786	3.9746575832492033E-4
32-33	0.0	0.0	0.0	0.07522039476299117	3.9746575832492033E-4
34-35	0.0	0.0	0.0	0.11218471028720875	3.9746575832492033E-4
36-37	0.0	0.0	0.0	0.1625634951548924	3.9746575832492033E-4
38-39	0.0	0.0	0.0	0.2316231706638473	3.9746575832492033E-4
40-41	0.0	0.0	0.0	0.31220935316422493	3.9746575832492033E-4
42-43	0.0	0.0	0.0	0.37878486768364905	3.9746575832492033E-4
44-45	0.0	0.0	0.0	0.44446608424684214	3.9746575832492033E-4
46-47	0.0	0.0	0.0	0.5258471982638695	3.9746575832492033E-4
48-49	0.0	0.0	0.0	0.6119979013807961	3.9746575832492033E-4
50-51	0.0	0.0	0.0	0.6990429024539536	3.9746575832492033E-4
52-53	0.0	0.0	0.0	0.7720772355461577	5.961986374873805E-4
54-55	0.0	0.0	0.0	0.8505767228153294	7.949315166498407E-4
56-57	0.0	0.0	0.0	0.936329960173931	7.949315166498407E-4
58-59	1.9873287916246017E-4	0.0	0.0	1.0259584886762005	8.942979562310707E-4
60-61	1.9873287916246017E-4	0.0	0.0	1.1195616747617194	9.936643958123007E-4
62-63	1.9873287916246017E-4	0.0	0.0	1.2081965388681766	9.936643958123007E-4
64-65	1.9873287916246017E-4	0.0	0.0	1.2928567453913846	9.936643958123007E-4
66-67	1.9873287916246017E-4	0.0	0.0	1.385466267081091	0.0010930308353935309
68-69	1.9873287916246017E-4	0.0	0.0	1.4890060971247325	0.0011923972749747608
70-71	1.9873287916246017E-4	0.0	0.0	1.6089413896992775	0.0011923972749747608
72-73	1.9873287916246017E-4	0.0	0.0	1.723610260976017	0.0013911301541372212
74-75	1.9873287916246017E-4	0.0	0.0	1.82804438897589	0.0017885959124621415
76-77	1.9873287916246017E-4	0.0	0.0	1.9399309999443548	0.0020866952312058318
78-79	1.9873287916246017E-4	0.0	0.0	2.0617542548709427	0.0021860616707870617
80-81	1.9873287916246017E-4	0.0	0.0	2.1957002154264407	0.0021860616707870617
82-83	1.9873287916246017E-4	0.0	0.0	2.3350119637193254	0.0023847945499495217
84-85	1.9873287916246017E-4	0.0	0.0	2.4641883351749243	0.0023847945499495217
86-87	1.9873287916246017E-4	0.0	0.0	2.5927685079930365	0.0023847945499495217
88-89	1.9873287916246017E-4	0.0	0.0	2.7388371741774447	0.0023847945499495217
90-91	1.9873287916246017E-4	0.0	0.0	2.8902716280992395	0.0023847945499495217
92-93	1.9873287916246017E-4	0.0	0.0	3.057704078793612	0.0023847945499495217
94-95	1.9873287916246017E-4	0.0	0.0	3.213610022496562	0.002583527429111982
96-97	1.9873287916246017E-4	0.0	0.0	3.3692178668807684	0.0027822603082744424
98-99	1.9873287916246017E-4	0.0	0.0	3.5383395470480217	0.0027822603082744424
100-101	1.9873287916246017E-4	0.0	0.0	3.7260427514169656	0.0027822603082744424
102-103	1.9873287916246017E-4	0.0	0.0	3.9160313838962777	0.0028816267478556723
104-105	1.9873287916246017E-4	0.0	0.0	4.11734779048785	0.0029809931874369023
106-107	1.9873287916246017E-4	0.0	0.0	4.310714881912924	0.0029809931874369023
108-109	1.9873287916246017E-4	0.0	0.0	4.51401861729612	0.0029809931874369023
110-111	1.9873287916246017E-4	0.0	0.0	4.738388037870537	0.0031797260665993627
112-113	1.9873287916246017E-4	0.0	0.0	4.966831482467786	0.0031797260665993627
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2410	0.0	26.45493	1
CTAACGC	115	9.985544E-4	25.89378	3
GGTATCA	1820	0.0	25.5366	1
GTATGAC	155	2.0836218E-4	23.065317	1
CATGGGT	680	0.0	22.771296	4
TATCAAC	2795	0.0	22.586416	2
CATGGGG	1795	0.0	22.229702	4
ATCAACG	2730	0.0	21.815271	3
AACGCAG	2800	0.0	21.252937	6
GTACATG	3980	0.0	21.109402	1
CAACGCA	2780	0.0	20.994453	5
TCAACGC	2810	0.0	20.982254	4
ACATGGG	3860	0.0	20.674774	3
TACATGG	4075	0.0	20.022404	2
ATGGGGG	1060	0.0	19.664616	5
ACGCAGA	2995	0.0	19.475685	7
GTAAGGC	185	6.904221E-4	19.315361	3
CGCAGAG	3050	0.0	19.130205	8
GTATAGG	160	0.006771135	18.620438	1
TTTTGCG	80	0.006850217	18.585207	86-87
>>END_MODULE