FastQCFastQC Report
Sun 1 Jan 2017
ERR1142459_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142459_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503188
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20580.408992265316343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20090.3992543542373824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7910.15719770741750597No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG1900.043.841041
GCGCAGA2950.042.3549081
GGTATCA18900.039.35091
GTATCAA27150.036.597641
ATCAACG32750.029.6100793
TATCAAC33650.028.641332
GCAACGC850.007207425327.9993211
GCGTACA850.007207425327.9993211
TGAGTCG850.007210924827.9965385
TCAACGC34800.027.8658074
CAACGCA34800.027.5238935
AACGCAG36800.026.6720396
ACGCAGA40450.023.9640187
CGCAGAG42400.022.9998728
ACACCGC1400.003157306221.245266
GCAGAGT46750.021.1141649
GAGTACT18750.021.08957312-13
CATGGGT6850.019.9756324
AACGGTT750.00462817819.8902182-83
GTATAAT1805.7783665E-419.8328511
Produced by FastQC (version 0.11.5)