##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142459_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	503188
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.594527293973623	27.0	14.0	33.0	14.0	33.0
2	26.170713530529344	27.0	27.0	33.0	14.0	33.0
3	25.41204678966907	27.0	14.0	33.0	14.0	33.0
4	30.577098420471078	33.0	27.0	33.0	27.0	33.0
5	32.10087680946287	33.0	33.0	33.0	33.0	33.0
6	35.71757076877827	37.0	37.0	37.0	33.0	37.0
7	35.75829312304745	37.0	37.0	37.0	33.0	37.0
8	36.0620483795321	37.0	37.0	37.0	33.0	37.0
9	36.21632272629713	37.0	37.0	37.0	37.0	37.0
10-11	36.190722950467816	37.0	37.0	37.0	37.0	37.0
12-13	36.23800050875617	37.0	37.0	37.0	37.0	37.0
14-15	36.23156951278648	37.0	37.0	37.0	37.0	37.0
16-17	36.23221042632177	37.0	37.0	37.0	37.0	37.0
18-19	36.2228083738086	37.0	37.0	37.0	37.0	37.0
20-21	36.2313260650095	37.0	37.0	37.0	37.0	37.0
22-23	36.22607752967082	37.0	37.0	37.0	37.0	37.0
24-25	36.2186300150242	37.0	37.0	37.0	37.0	37.0
26-27	36.175464240005724	37.0	37.0	37.0	37.0	37.0
28-29	36.158510338084376	37.0	37.0	37.0	37.0	37.0
30-31	36.101317996454604	37.0	37.0	37.0	37.0	37.0
32-33	36.07254942486705	37.0	37.0	37.0	37.0	37.0
34-35	36.00791155591946	37.0	37.0	37.0	37.0	37.0
36-37	35.9403234973807	37.0	37.0	37.0	37.0	37.0
38-39	35.89326156426624	37.0	37.0	37.0	37.0	37.0
40-41	35.78010902485751	37.0	37.0	37.0	33.0	37.0
42-43	35.75071444470059	37.0	37.0	37.0	33.0	37.0
44-45	35.69009197357647	37.0	37.0	37.0	33.0	37.0
46-47	35.574433412561504	37.0	37.0	37.0	33.0	37.0
48-49	35.47195580975699	37.0	37.0	37.0	33.0	37.0
50-51	35.384995270157475	37.0	37.0	37.0	33.0	37.0
52-53	35.32632236857795	37.0	37.0	37.0	33.0	37.0
54-55	35.28015572708411	37.0	37.0	37.0	33.0	37.0
56-57	35.22959609529639	37.0	37.0	37.0	33.0	37.0
58-59	35.18716960658839	37.0	37.0	37.0	33.0	37.0
60-61	35.15883824733499	37.0	37.0	37.0	33.0	37.0
62-63	35.15549953496506	37.0	37.0	37.0	33.0	37.0
64-65	35.12664749556826	37.0	37.0	37.0	33.0	37.0
66-67	35.084633377584524	37.0	37.0	37.0	33.0	37.0
68-69	35.04276234727378	37.0	37.0	37.0	33.0	37.0
70-71	35.01704830003895	37.0	37.0	37.0	33.0	37.0
72-73	34.987239361828976	37.0	37.0	37.0	27.0	37.0
74-75	34.89782049651423	37.0	37.0	37.0	27.0	37.0
76-77	34.85049623599927	37.0	37.0	37.0	27.0	37.0
78-79	34.83980738809352	37.0	37.0	37.0	27.0	37.0
80-81	34.809096202612146	37.0	37.0	37.0	27.0	37.0
82-83	34.75861407664729	37.0	37.0	37.0	27.0	37.0
84-85	34.68776679889028	37.0	37.0	37.0	27.0	37.0
86-87	34.52869404675787	37.0	37.0	37.0	27.0	37.0
88-89	34.444354793834506	37.0	37.0	37.0	27.0	37.0
90-91	34.40915423261286	37.0	37.0	37.0	27.0	37.0
92-93	34.36467185226993	37.0	37.0	37.0	27.0	37.0
94-95	34.383599171681354	37.0	37.0	37.0	27.0	37.0
96-97	34.350007353116524	37.0	37.0	37.0	27.0	37.0
98-99	34.28583253177739	37.0	37.0	37.0	27.0	37.0
100-101	34.241237867357725	37.0	37.0	37.0	27.0	37.0
102-103	34.13736714707028	37.0	37.0	37.0	27.0	37.0
104-105	34.04554560124645	37.0	37.0	37.0	22.0	37.0
106-107	33.9769837515998	37.0	37.0	37.0	22.0	37.0
108-109	33.89739322082403	37.0	37.0	37.0	22.0	37.0
110-111	33.82517369253639	37.0	37.0	37.0	22.0	37.0
112-113	33.77877950189591	37.0	33.0	37.0	22.0	37.0
114-115	33.65578769763985	37.0	33.0	37.0	22.0	37.0
116-117	33.53436190847158	37.0	33.0	37.0	22.0	37.0
118-119	33.407888304172594	37.0	33.0	37.0	22.0	37.0
120-121	33.333003767975384	37.0	33.0	37.0	22.0	37.0
122-123	33.20949823922669	37.0	33.0	37.0	14.0	37.0
124-125	31.92888940117809	37.0	30.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	8.0
15	180.0
16	76.0
17	18.0
18	51.0
19	252.0
20	962.0
21	2339.0
22	3790.0
23	4995.0
24	4477.0
25	3503.0
26	3446.0
27	4210.0
28	5366.0
29	6919.0
30	9054.0
31	12743.0
32	17755.0
33	26774.0
34	44327.0
35	100196.0
36	251747.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.54310046637006	12.330975639817588	11.797612089838857	22.328311803973488
2	18.707160558599487	19.806751764254642	35.44229083819733	26.04379683894854
3	22.30471894034997	23.9756749505649	28.245078847739897	25.47452726134523
4	15.407561388586375	17.211658465623188	34.66636724246206	32.71441290332838
5	16.386118740336496	34.718054302419404	31.66985305515698	17.22597390208712
6	30.79632018156633	33.68019603439743	18.38140455545464	17.14207922858159
7	27.14159371180428	31.12378643188616	22.297258354615284	19.437361501694276
8	26.164574671892016	31.44232374380947	21.32205060534035	21.071050978958166
9	26.966660572191703	15.864249544901707	20.706177412815883	36.4629124700907
10-11	25.795825814606072	24.53953591898058	26.49854527532453	23.166092991088817
12-13	26.920852643544755	23.560677122665883	26.30170035851411	23.216769875275244
14-15	25.175779231619195	23.92475575729151	24.835647908932646	26.06381710215665
16-17	24.119613345310302	26.03470273535935	25.719611755447268	24.12607216388308
18-19	24.029189885291384	24.67040151990906	27.883415343768135	23.416993251031425
20-21	25.249311387836705	23.798654970527796	27.848350311813125	23.10368332982237
22-23	25.114470138397575	23.77848835822794	27.582136298957842	23.52490520441664
24-25	24.087617351765147	24.45388204806156	27.766162945062284	23.69233765511101
26-27	23.972948480488405	24.484486911452578	27.7030652559282	23.839499352130815
28-29	23.730096902151878	24.326295539639258	28.161840107474738	23.78176745073412
30-31	24.039027162809923	24.263794049142668	28.034750431250348	23.66242835679706
32-33	23.758714436751273	24.251770709953338	27.85469844272916	24.13481641056623
34-35	24.03259222456838	24.365271394857782	27.880660787479815	23.721475593094027
36-37	24.005437353185503	24.47365387749262	27.511993576927818	24.008915192394063
38-39	23.950988497340955	24.399627972050208	27.4976748253138	24.15170870529504
40-41	23.809769868801666	24.582272971632822	27.76012854515228	23.847828614413235
42-43	24.147806032157064	24.364226782713764	27.616003211532902	23.871963973596273
44-45	24.06555800217811	24.459247835798944	27.2747959013331	24.20039826068984
46-47	24.01239364736138	24.537871575897178	27.148964248023994	24.300770528717447
48-49	24.15487261228781	24.766520454179577	27.029567782108284	24.049039151424328
50-51	23.852317622836793	25.081083014698287	26.762263805973113	24.304335556491807
52-53	24.006136871308538	24.77145718896317	26.800023053013984	24.42238288671431
54-55	24.035946803182906	24.838231436361756	26.724405192492668	24.40141656796267
56-57	24.191157181808787	24.641883351749247	26.52507611469272	24.641883351749247
58-59	23.943039182174456	24.75128580172818	26.749644268146298	24.556030747951063
60-61	24.070772893576343	24.764550753497208	26.905106848944826	24.25956950398162
62-63	23.66729731233654	24.983505171029517	26.9227406058968	24.42645691073714
64-65	23.776600395875896	24.685505218725407	26.92929879090916	24.608595594489536
66-67	23.97465556854047	24.685082719199915	26.86714445848935	24.473117253770262
68-69	24.018060844058283	24.553745319840694	26.931782951898693	24.496410884202326
70-71	23.689157929044413	24.538144788826443	27.20603432514289	24.566662956986253
72-73	23.9872572477881	24.64883900251993	27.24687393181077	24.11702981788119
74-75	23.878039709389164	24.77789511995752	27.194945137558847	24.149120033094473
76-77	23.88506729756059	24.73915982425098	27.080061233821745	24.295711644366687
78-79	23.96879480827647	24.702818344328286	27.13542023766583	24.192966609729414
80-81	23.597641438190102	24.838032703482597	27.344650508358704	24.2196753499686
82-83	23.815651406631318	24.875295118325553	27.235645524138096	24.07340795090503
84-85	23.75050333818858	24.800628364909002	27.054788051124245	24.394080245778174
86-87	23.619780359852935	24.922524467529986	27.114345122626442	24.34335004999064
88-89	23.861735940467927	24.597719238657202	27.025796767410753	24.51474805346412
90-91	24.043275388018664	24.709999771124465	26.94024258816435	24.306482252692522
92-93	23.855864208891646	24.989479611337547	26.79003293901358	24.36462324075723
94-95	23.395553347410257	25.02804620544032	27.030381319090797	24.546019128058628
96-97	23.76477578956573	24.819053713522578	26.813735621676194	24.6024348752355
98-99	23.542393697782938	24.952900307638497	26.648489232652604	24.85621676192596
100-101	23.731885498064344	25.107315754747727	26.43952161021328	24.72127713697465
102-103	23.979208566182024	25.006061352814456	26.51643123444915	24.49829884655437
104-105	23.790780959346232	25.170616245607896	26.33054835683737	24.7080544382085
106-107	23.68834482875123	25.14689325607671	26.54810536765298	24.616656547519085
108-109	23.879618137571036	25.10784225071075	26.30665258250019	24.705887029218022
110-111	23.805974969320275	25.21878244970761	26.214445057210856	24.760797523761262
112-113	23.662726456115806	25.39190123770837	26.142614688744565	24.802757617431258
114-115	23.701380001112902	25.477753841506555	26.223598337003267	24.597267820377276
116-117	23.65817910818532	25.450254626754443	26.15198112035772	24.739585144702524
118-119	23.538021574441363	25.35861348044866	26.293651676908038	24.809713268201943
120-121	23.627989705575484	25.26267674910818	26.434710891620377	24.67462265369596
122-123	23.533649451099787	25.505377711710135	26.262251882000363	24.69872095518971
124-125	23.509105940523224	25.517202318020303	26.320182516276226	24.65350922518025
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	111.0
1	110.0
2	207.5
3	302.5
4	243.5
5	211.5
6	290.0
7	496.0
8	874.0
9	1107.5
10	976.5
11	794.0
12	736.5
13	731.0
14	745.5
15	743.5
16	708.5
17	701.5
18	701.0
19	700.5
20	708.0
21	712.0
22	791.0
23	835.0
24	849.5
25	977.0
26	1226.0
27	1502.0
28	1750.0
29	2101.0
30	2713.0
31	3338.0
32	4086.5
33	4901.5
34	5620.0
35	6387.0
36	7233.5
37	8190.5
38	9094.0
39	9847.0
40	10444.0
41	11006.5
42	11618.5
43	12675.0
44	14231.0
45	15484.0
46	16773.0
47	18382.0
48	20010.5
49	21343.5
50	21952.5
51	21639.0
52	21044.5
53	21005.5
54	21028.0
55	20458.0
56	19397.0
57	17932.5
58	16748.0
59	15294.5
60	13419.5
61	11297.5
62	9123.0
63	7332.0
64	5608.0
65	4435.0
66	3531.0
67	2734.5
68	2206.0
69	1831.5
70	1528.5
71	1366.5
72	1243.0
73	1140.5
74	906.0
75	761.0
76	639.5
77	430.0
78	298.0
79	199.5
80	132.5
81	94.0
82	66.5
83	39.0
84	22.0
85	13.5
86	6.0
87	3.5
88	2.0
89	4.0
90	4.0
91	1.5
92	0.5
93	0.5
94	0.5
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030604863391018863
2	1.9873287916246017E-4
3	5.961986374873805E-4
4	0.0
5	0.001192397274974761
6	0.0021860616707870617
7	0.004570856220736583
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	3.9746575832492033E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	9.936643958123008E-5
36-37	3.9746575832492033E-4
38-39	0.0
40-41	0.0036765582645055125
42-43	2.9809931874369026E-4
44-45	0.0
46-47	0.005365787737386424
48-49	0.007750582287335946
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	1.9873287916246017E-4
62-63	0.0
64-65	0.0
66-67	0.007949315166498406
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.07651215847754715
76-77	0.03934911007416711
78-79	4.968321979061503E-4
80-81	0.0
82-83	0.0
84-85	0.05872556579250698
86-87	0.21741376980373142
88-89	0.23977121870950818
90-91	0.14537310110733961
92-93	0.11774923090375763
94-95	9.936643958123008E-5
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.002384794549949522
106-107	0.0032790925061805926
108-109	0.0044714897811553534
110-111	0.0010930308353935309
112-113	0.0
114-115	0.0
116-117	9.936643958123008E-5
118-119	0.0
120-121	5.961986374873805E-4
122-123	0.0
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	503188.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.53266177238467
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.71601610955338	71.44536919144092
2	8.261115673527494	13.30579268797471
3	1.5654889139490857	3.782189676464387
4	0.5464285109074459	1.7602136980678862
5	0.25798877398177905	1.0388261338073406
6	0.16346062566274155	0.7898351567759969
7	0.09159794044458493	0.5163638169809556
8	0.07013536351208616	0.4518550006401667
9	0.05671261838245459	0.4110496302978487
>10	0.24981802537446127	3.546513530136285
>50	0.014441462555150327	0.8316657486328729
>100	0.005946484380658941	1.0125022839090174
>500	2.831659228885189E-4	0.18038047445315986
>1k	5.663318457770378E-4	0.9274429704184591
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2058	0.408992265316343	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2009	0.3992543542373824	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	791	0.15719770741750597	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	9.936643958123008E-5
16-17	0.0	0.0	0.0	2.9809931874369026E-4	1.9873287916246017E-4
18-19	0.0	0.0	0.0	5.961986374873804E-4	1.9873287916246017E-4
20-21	0.0	0.0	0.0	0.001291763714555991	1.9873287916246017E-4
22-23	0.0	0.0	0.0	0.0027822603082744424	1.9873287916246017E-4
24-25	0.0	0.0	0.0	0.006359452133198724	1.9873287916246017E-4
26-27	0.0	0.0	0.0	0.010930308353935308	1.9873287916246017E-4
28-29	0.0	0.0	0.0	0.020270753674570936	1.9873287916246017E-4
30-31	0.0	0.0	0.0	0.041733904624116636	1.9873287916246017E-4
32-33	0.0	0.0	0.0	0.07522039476299117	1.9873287916246017E-4
34-35	0.0	0.0	0.0	0.11208534384762753	1.9873287916246017E-4
36-37	0.0	0.0	0.0	0.16246412871531118	1.9873287916246017E-4
38-39	0.0	0.0	0.0	0.23082823914719747	3.9746575832492033E-4
40-41	0.0	0.0	0.0	0.3112156887684126	3.9746575832492033E-4
42-43	0.0	0.0	0.0	0.3777912032878368	3.9746575832492033E-4
44-45	0.0	0.0	0.0	0.44386988560935475	3.9746575832492033E-4
46-47	0.0	0.0	0.0	0.5257478318242883	3.9746575832492033E-4
48-49	0.0	0.0	0.0	0.6128921993370271	5.961986374873804E-4
50-51	0.0	0.0	0.0	0.7007321319268345	5.961986374873804E-4
52-53	0.0	0.0	0.0	0.774760129414851	5.961986374873804E-4
54-55	0.0	0.0	0.0	0.8534583495631851	5.961986374873804E-4
56-57	0.0	0.0	0.0	0.9391122204822054	5.961986374873804E-4
58-59	0.0	0.0	0.0	1.0302312455781935	5.961986374873804E-4
60-61	0.0	0.0	0.0	1.1247287296199433	5.961986374873804E-4
62-63	0.0	0.0	0.0	1.2139597923638878	5.961986374873804E-4
64-65	0.0	0.0	0.0	1.2994149304037457	5.961986374873804E-4
66-67	0.0	0.0	0.0	1.3926206507309395	6.955650770686106E-4
68-69	0.0	0.0	0.0	1.4966573129724874	7.949315166498407E-4
70-71	0.0	0.0	0.0	1.6182818350199129	8.942979562310707E-4
72-73	0.0	0.0	0.0	1.7346399357695335	9.936643958123007E-4
74-75	0.0	0.0	0.0	1.838477865131919	9.936643958123007E-4
76-77	0.0	1.9873287916246017E-4	0.0	1.9507619418587088	9.936643958123007E-4
78-79	0.0	1.9873287916246017E-4	0.0	2.073876960499853	9.936643958123007E-4
80-81	0.0	1.9873287916246017E-4	0.0	2.208419119692839	9.936643958123007E-4
82-83	0.0	1.9873287916246017E-4	0.0	2.3479296008648856	9.936643958123007E-4
84-85	0.0	1.9873287916246017E-4	0.0	2.477702170957972	9.936643958123007E-4
86-87	0.0	1.9873287916246017E-4	0.0	2.607176641732315	9.936643958123007E-4
88-89	0.0	1.9873287916246017E-4	0.0	2.7531459414771415	9.936643958123007E-4
90-91	0.0	1.9873287916246017E-4	0.0	2.906965189948886	9.936643958123007E-4
92-93	0.0	1.9873287916246017E-4	0.0	3.075590037918233	9.936643958123007E-4
94-95	0.0	1.9873287916246017E-4	0.0	3.232986478214902	0.0011923972749747608
96-97	0.0	1.9873287916246017E-4	0.0	3.3922708808636135	0.0011923972749747608
98-99	0.0	1.9873287916246017E-4	0.0	3.564274187778723	0.001291763714555991
100-101	0.0	1.9873287916246017E-4	0.0	3.753467888741385	0.0013911301541372212
102-103	0.0	1.9873287916246017E-4	0.0	3.947331812364365	0.0013911301541372212
104-105	0.0	1.9873287916246017E-4	0.0	4.153219075176674	0.0015898630332996813
106-107	0.0	1.9873287916246017E-4	0.0	4.352945618734946	0.0015898630332996813
108-109	0.0	1.9873287916246017E-4	0.0	4.561217676097204	0.0015898630332996813
110-111	0.0	1.9873287916246017E-4	0.0	4.790456052211102	0.0016892294728809115
112-113	0.0	1.9873287916246017E-4	0.0	5.026053880458198	0.0017885959124621415
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCAACG	190	0.0	43.84104	1
GCGCAGA	295	0.0	42.354908	1
GGTATCA	1890	0.0	39.3509	1
GTATCAA	2715	0.0	36.59764	1
ATCAACG	3275	0.0	29.610079	3
TATCAAC	3365	0.0	28.64133	2
GCAACGC	85	0.0072074253	27.999321	1
GCGTACA	85	0.0072074253	27.999321	1
TGAGTCG	85	0.0072109248	27.996538	5
TCAACGC	3480	0.0	27.865807	4
CAACGCA	3480	0.0	27.523893	5
AACGCAG	3680	0.0	26.672039	6
ACGCAGA	4045	0.0	23.964018	7
CGCAGAG	4240	0.0	22.999872	8
ACACCGC	140	0.0031573062	21.24526	6
GCAGAGT	4675	0.0	21.114164	9
GAGTACT	1875	0.0	21.089573	12-13
CATGGGT	685	0.0	19.975632	4
AACGGTT	75	0.004628178	19.89021	82-83
GTATAAT	180	5.7783665E-4	19.832851	1
>>END_MODULE