FastQCFastQC Report
Sun 1 Jan 2017
ERR1142458_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142458_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729611
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15630.21422374388544033No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14830.2032589969175355No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18600.035.8667071
GTATCAA25400.030.0166821
ATCAACG29500.024.0193943
TCAACGC30200.023.0683214
CAACGCA30000.023.023635
TATCAAC31200.022.8999142
AACGCAG30150.022.6959866
CATGGGG24600.022.2684424
GTACATG47300.021.4079061
ACATGGG46400.020.917353
TACATGG47800.020.8015562
ACGCAGA33750.020.1056847
ATGGGGG13550.019.774725
CGCAGAG34900.019.103388
CAGAGTA36450.017.73284310-11
GCAGAGT38200.017.6222469
GTATTAC2452.3076468E-417.0183871
GTACATA2502.6923852E-416.6780191
TGGGGGG8950.016.6209376
GAGTACA38750.016.52657712-13
Produced by FastQC (version 0.11.5)