FastQCFastQC Report
Sun 1 Jan 2017
ERR1142458_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142458_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729611
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26650.36526313336832916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24910.3414148087131362No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11040.15131350815708644No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG1950.051.8772351
GCGCAGA5000.047.6049921
GTATCAA37550.041.202721
GGTATCA25050.041.0961271
ATCAACG44500.034.7557753
CAACGCA45650.034.4014555
TATCAAC45200.034.085922
TCAACGC46600.033.827794
AACGCAG47950.032.999456
ACGCAGA51800.030.2002017
CGCAGAG55700.028.0837258
GCAGAGT59500.026.3901049
GAGTACT24650.024.12888112-13
CAGAGTA58500.022.36767810-11
GTACTTT27800.021.2878814-15
GTATACA1450.003863693720.5193941
AGAGTAC56600.020.38634510-11
GTATAGA2357.3815754E-620.2574421
GTACATG50000.019.8750841
GGTCTAG2107.135581E-519.8354131
Produced by FastQC (version 0.11.5)