##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142458_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	729611
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.50608063749039	27.0	14.0	33.0	14.0	33.0
2	25.87574337557959	27.0	27.0	33.0	14.0	33.0
3	25.102738308495898	27.0	14.0	33.0	14.0	33.0
4	30.461108727801527	33.0	27.0	33.0	27.0	33.0
5	32.07940121516808	33.0	33.0	33.0	33.0	33.0
6	35.66017507959721	37.0	37.0	37.0	33.0	37.0
7	35.680403667159624	37.0	37.0	37.0	33.0	37.0
8	36.010633063372126	37.0	37.0	37.0	33.0	37.0
9	36.16929158140434	37.0	37.0	37.0	37.0	37.0
10-11	36.1511709664465	37.0	37.0	37.0	37.0	37.0
12-13	36.21333628467772	37.0	37.0	37.0	37.0	37.0
14-15	36.19741958386044	37.0	37.0	37.0	37.0	37.0
16-17	36.2038558903306	37.0	37.0	37.0	37.0	37.0
18-19	36.18921452664502	37.0	37.0	37.0	37.0	37.0
20-21	36.206106404645766	37.0	37.0	37.0	37.0	37.0
22-23	36.20289921615765	37.0	37.0	37.0	37.0	37.0
24-25	36.187376560934524	37.0	37.0	37.0	37.0	37.0
26-27	36.153281680237825	37.0	37.0	37.0	37.0	37.0
28-29	36.1258965393888	37.0	37.0	37.0	37.0	37.0
30-31	36.07208567305044	37.0	37.0	37.0	37.0	37.0
32-33	36.044695735124606	37.0	37.0	37.0	37.0	37.0
34-35	35.99063473549604	37.0	37.0	37.0	37.0	37.0
36-37	35.92860853249197	37.0	37.0	37.0	35.0	37.0
38-39	35.88946027403644	37.0	37.0	37.0	35.0	37.0
40-41	35.78008966421833	37.0	37.0	37.0	33.0	37.0
42-43	35.75590006181376	37.0	37.0	37.0	33.0	37.0
44-45	35.70638943217688	37.0	37.0	37.0	33.0	37.0
46-47	35.611810266018466	37.0	37.0	37.0	33.0	37.0
48-49	35.50468605873541	37.0	37.0	37.0	33.0	37.0
50-51	35.42680894339587	37.0	37.0	37.0	33.0	37.0
52-53	35.37068040366716	37.0	37.0	37.0	33.0	37.0
54-55	35.321217744798254	37.0	37.0	37.0	33.0	37.0
56-57	35.27867247067273	37.0	37.0	37.0	33.0	37.0
58-59	35.229709393087546	37.0	37.0	37.0	33.0	37.0
60-61	35.199106099003444	37.0	37.0	37.0	33.0	37.0
62-63	35.187229222147145	37.0	37.0	37.0	33.0	37.0
64-65	35.15405949197586	37.0	37.0	37.0	33.0	37.0
66-67	35.11529842614763	37.0	37.0	37.0	33.0	37.0
68-69	35.08200191608953	37.0	37.0	37.0	33.0	37.0
70-71	35.0457401272733	37.0	37.0	37.0	33.0	37.0
72-73	35.02322470467139	37.0	37.0	37.0	33.0	37.0
74-75	34.927933515256754	37.0	37.0	37.0	27.0	37.0
76-77	34.87989695879037	37.0	37.0	37.0	27.0	37.0
78-79	34.86636440514192	37.0	37.0	37.0	27.0	37.0
80-81	34.83102228447762	37.0	37.0	37.0	27.0	37.0
82-83	34.7871112140579	37.0	37.0	37.0	27.0	37.0
84-85	34.7114524040893	37.0	37.0	37.0	27.0	37.0
86-87	34.54651382723122	37.0	37.0	37.0	27.0	37.0
88-89	34.47995849843272	37.0	37.0	37.0	27.0	37.0
90-91	34.44237751349692	37.0	37.0	37.0	27.0	37.0
92-93	34.390180520852894	37.0	37.0	37.0	27.0	37.0
94-95	34.40069502789843	37.0	37.0	37.0	27.0	37.0
96-97	34.37273903491038	37.0	37.0	37.0	27.0	37.0
98-99	34.309107181772205	37.0	37.0	37.0	27.0	37.0
100-101	34.253944910370045	37.0	37.0	37.0	27.0	37.0
102-103	34.157869056250526	37.0	37.0	37.0	27.0	37.0
104-105	34.070260042680275	37.0	37.0	37.0	27.0	37.0
106-107	34.00625401755182	37.0	37.0	37.0	22.0	37.0
108-109	33.91531172090333	37.0	37.0	37.0	22.0	37.0
110-111	33.84700477377672	37.0	33.0	37.0	22.0	37.0
112-113	33.783007657505166	37.0	33.0	37.0	22.0	37.0
114-115	33.66138599884048	37.0	33.0	37.0	22.0	37.0
116-117	33.550630404420986	37.0	33.0	37.0	22.0	37.0
118-119	33.43289095147962	37.0	33.0	37.0	22.0	37.0
120-121	33.355329072615405	37.0	33.0	37.0	22.0	37.0
122-123	33.215008408590336	37.0	33.0	37.0	18.0	37.0
124-125	31.93995293382364	37.0	30.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	210.0
16	115.0
17	35.0
18	84.0
19	301.0
20	1138.0
21	2862.0
22	4771.0
23	6389.0
24	5846.0
25	4788.0
26	5040.0
27	6059.0
28	8052.0
29	10605.0
30	13963.0
31	19232.0
32	27082.0
33	41106.0
34	68238.0
35	152030.0
36	351664.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	54.38647001242113	11.609373157737435	11.705067987200474	22.299088842640955
2	18.674360274667286	19.521114019818807	35.4405778429572	26.363947862556707
3	22.467787540997985	23.497791282728144	28.237453211240542	25.796967965033325
4	15.646693923200171	16.91408161335287	34.51496756490788	32.924256898539085
5	16.567136562065254	34.74927870942098	31.719104434652102	16.964480293861666
6	31.191809266657987	33.4962068516526	18.439566614812126	16.87241726687728
7	27.234149290064185	30.867918631857265	22.362258471736208	19.535673606342343
8	26.10898136130075	31.5517446968316	21.255847293968976	21.083426647898676
9	26.877884242425072	15.873115948087404	20.71076231032701	36.538237499160516
10-11	25.79888461111469	24.548355219425147	26.634329800400486	23.01843036905968
12-13	26.93716240572031	23.335722734443422	26.443200554816197	23.283914305020073
14-15	25.022786114792677	23.822626029486944	24.830491864843047	26.324095990877332
16-17	24.27814273633484	25.937862778932885	25.676627682422552	24.107366802309723
18-19	24.050966885093565	24.857286965245866	27.558932088469064	23.532814061191512
20-21	25.147904461222065	24.088844786149302	27.490321877661955	23.272928874966677
22-23	25.0782438026865	23.843338329149592	27.418670646872545	23.65974722129136
24-25	24.336461484270387	24.420478857911956	27.38034377222931	23.862715885588347
26-27	24.073787264720515	24.60427542896146	27.44955873746421	23.872378568853815
28-29	23.919321391810154	24.457347819591536	27.718469156852077	23.904861631746233
30-31	24.246344970127918	24.41670972614174	27.668031320799713	23.668913982930633
32-33	23.872652687528014	24.464543434789224	27.55577972371579	24.10702415396698
34-35	24.152029270452207	24.452121305986285	27.540005989505346	23.855843434056162
36-37	24.260426803360698	24.52618522224202	27.295335864365892	23.918052110031386
38-39	24.09256439390305	24.553083766555055	27.28748607134487	24.066865768197026
40-41	23.993928138213665	24.535187329957033	27.501559084149424	23.969325447679875
42-43	24.15987708510226	24.47059081523635	27.28167974556287	24.087852354098523
44-45	24.076254332788295	24.59269391497661	27.0765517515498	24.254500000685297
46-47	24.10983463535688	24.643368452217366	26.954594456045655	24.2922024563801
48-49	24.231682861603538	24.885579995312174	26.82163912225224	24.06109802083205
50-51	23.954956819455848	25.071305120125658	26.71115155884437	24.262586501574127
52-53	24.06049250902193	24.84618515894086	26.65201045488623	24.441311877150977
54-55	24.116549777895344	24.869827894590404	26.658589303066975	24.355033024447273
56-57	24.169660271021133	24.728793836715727	26.42360106961107	24.67794482265207
58-59	24.02821503513516	24.774845773980932	26.658726362404078	24.538212828479832
60-61	24.138410742162606	24.83343864059067	26.776940040651798	24.251210576594925
62-63	23.776505562553197	24.969607091998338	26.80126807298684	24.452619272461625
64-65	23.868472377746496	24.66046975717197	26.87946042480171	24.59159744027982
66-67	24.019477827009208	24.744653363900227	26.725196097581033	24.51067271150953
68-69	24.103529140870958	24.655741210042066	26.76206910257658	24.4786605465104
70-71	23.772393782440233	24.675751873258488	27.059830512423744	24.492023831877535
72-73	23.917265501753672	24.91163099240554	26.971221650989364	24.19988185485142
74-75	23.881687142608293	24.838800823518277	26.989906277475185	24.289605756398245
76-77	23.92658477426573	24.688447357921163	26.999469376730183	24.385498491082924
78-79	23.810424487636528	24.90087156271373	26.941416566955727	24.34728738269402
80-81	23.756563429005322	24.98461508941066	27.04550781169692	24.213313669887103
82-83	23.848119066187323	24.95555850994571	26.986229648401682	24.210092775465284
84-85	23.770957190759294	25.008057161273513	26.81340985922254	24.40757578874466
86-87	23.58901426711422	25.086113598532084	26.9135210710499	24.411351063303798
88-89	23.77484330728943	24.71991070661973	26.900317678372115	24.604928307718726
90-91	24.04187043032964	24.816389667037708	26.72283520981195	24.418904692820703
92-93	23.8517173812817	25.112584798788117	26.612892160091327	24.422805659838858
94-95	23.532640725867243	25.051054673044504	26.758473705130136	24.657830895958114
96-97	23.70345293587953	24.924240451418633	26.677229372912414	24.695077239789423
98-99	23.461269087225933	25.120509422144128	26.523037618676256	24.895183871953684
100-101	23.712978559807897	25.242560761830617	26.287021440192106	24.757439238169383
102-103	23.821323965784508	25.086724295549274	26.469721536544817	24.622230202121404
104-105	23.675801105123824	25.329580599820588	26.375288427998022	24.619329867057566
106-107	23.78298919263427	25.168209760209432	26.336803294978722	24.711997752177577
108-109	23.70571142696583	25.23513206047232	26.247961183678502	24.811195328883347
110-111	23.57041285896869	25.54393972101438	26.148237237144627	24.737410182872296
112-113	23.511638393609747	25.61090773028367	26.053403800107176	24.824050075999402
114-115	23.565381803030522	25.5609671187572	26.15265268249292	24.72099839571936
116-117	23.611312893189513	25.64267211249846	26.019928454927978	24.726086539384053
118-119	23.49258714575301	25.356114422616983	26.243984808343075	24.90731362328693
120-121	23.454266349600125	25.46583425278061	26.3183503402526	24.761549057366658
122-123	23.371837869768957	25.706575147578643	26.168328054264535	24.753258928387865
124-125	23.397605025143537	25.517159143708085	26.196767866712534	24.888467964435844
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	147.0
1	151.5
2	312.0
3	483.5
4	374.5
5	320.5
6	439.5
7	775.5
8	1335.0
9	1632.5
10	1411.0
11	1072.0
12	962.0
13	945.0
14	968.5
15	939.5
16	889.5
17	910.5
18	891.5
19	848.0
20	855.5
21	883.0
22	919.0
23	974.5
24	1082.0
25	1214.5
26	1406.0
27	1718.0
28	2090.5
29	2665.5
30	3506.0
31	4320.5
32	5399.0
33	6586.0
34	7692.5
35	9046.0
36	10551.5
37	12111.0
38	13575.5
39	14844.0
40	15760.0
41	16648.0
42	17856.5
43	19616.5
44	21647.5
45	23350.0
46	25415.0
47	27466.5
48	29297.5
49	30990.5
50	31724.0
51	31177.0
52	30358.5
53	30358.0
54	30144.5
55	29312.5
56	28058.0
57	25780.5
58	23615.0
59	21466.5
60	18921.0
61	16113.5
62	13128.5
63	10479.5
64	8102.0
65	6496.5
66	5240.5
67	3996.5
68	3253.0
69	2780.0
70	2352.5
71	2069.0
72	1826.5
73	1698.5
74	1391.0
75	1139.5
76	956.5
77	724.5
78	538.0
79	366.0
80	270.5
81	199.5
82	130.0
83	81.5
84	50.0
85	27.0
86	16.5
87	13.0
88	7.0
89	7.0
90	7.0
91	4.0
92	3.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028645401453651326
2	1.370593370988102E-4
3	2.741186741976204E-4
4	0.0
5	0.002192949393580963
6	8.223560225928611E-4
7	0.005208254809754787
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.0558900564821527E-4
22-23	6.85296685494051E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	2.0558900564821527E-4
36-37	1.370593370988102E-4
38-39	0.0
40-41	0.002192949393580963
42-43	6.85296685494051E-5
44-45	0.0
46-47	0.005687962489600623
48-49	0.008497678900126232
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.008703267905774447
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.07456027938175273
76-77	0.03837661438766685
78-79	2.741186741976204E-4
80-81	0.0
82-83	0.0
84-85	0.06112846434606934
86-87	0.2060001836595117
88-89	0.22936880063485887
90-91	0.1411711172117745
92-93	0.11334807178071604
94-95	1.370593370988102E-4
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0013020637024386968
106-107	0.002192949393580963
108-109	0.002878246079075014
110-111	4.7970767984583567E-4
112-113	0.0
114-115	6.85296685494051E-5
116-117	1.370593370988102E-4
118-119	0.0
120-121	8.223560225928611E-4
122-123	0.0
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	729611.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.93313215490086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.63307807369173	70.04786410805147
2	8.90421362668518	14.234833691145916
3	1.8613463869913232	4.463497402122741
4	0.6466454818105903	2.0675359501974184
5	0.3160345786026314	1.2630816868481272
6	0.18155156091415528	0.8707190946887825
7	0.11091945378370728	0.6206297550489746
8	0.0745086584660255	0.47645683550793516
9	0.043430241528463684	0.31243637120525564
>10	0.2083791671991983	2.8179466712422783
>50	0.011367341207986425	0.6039117315182068
>100	0.007510497081375706	1.0028882247868607
>500	4.0597281508772246E-4	0.20249223496135327
>1k	6.089592226315836E-4	1.0157062426747367
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2665	0.36526313336832916	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2491	0.3414148087131362	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1104	0.15131350815708644	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.370593370988102E-4	0.0
5	0.0	0.0	0.0	1.370593370988102E-4	0.0
6	0.0	0.0	0.0	1.370593370988102E-4	0.0
7	0.0	0.0	0.0	1.370593370988102E-4	0.0
8	0.0	0.0	0.0	1.370593370988102E-4	0.0
9	0.0	0.0	0.0	1.370593370988102E-4	0.0
10-11	0.0	0.0	0.0	2.741186741976204E-4	0.0
12-13	0.0	0.0	0.0	4.7970767984583567E-4	2.055890056482153E-4
14-15	0.0	6.85296685494051E-5	0.0	5.482373483952407E-4	3.426483427470255E-4
16-17	0.0	1.370593370988102E-4	0.0	6.852966854940509E-4	4.111780112964306E-4
18-19	0.0	1.370593370988102E-4	0.0	6.852966854940509E-4	4.111780112964306E-4
20-21	0.0	1.370593370988102E-4	0.0	0.0010964746967904815	4.111780112964306E-4
22-23	0.0	1.370593370988102E-4	0.0	0.0023300087306797734	4.7970767984583567E-4
24-25	0.0	1.370593370988102E-4	0.0	0.005139725141205382	6.852966854940509E-4
26-27	0.0	1.370593370988102E-4	0.0	0.008840327242873258	6.852966854940509E-4
28-29	0.0	1.370593370988102E-4	0.0	0.01857154017688878	8.223560225928612E-4
30-31	0.0	1.370593370988102E-4	0.0	0.03645778366828351	8.223560225928612E-4
32-33	0.0	1.370593370988102E-4	0.0	0.06469200711063841	8.223560225928612E-4
34-35	0.0	1.370593370988102E-4	0.0	0.09573594696351892	0.0013705933709881018
36-37	0.0	1.370593370988102E-4	0.0	0.13829287113269947	0.0013705933709881018
38-39	0.0	1.370593370988102E-4	0.0	0.20079192884975694	0.0013705933709881018
40-41	0.0	1.370593370988102E-4	0.0	0.27405014452907095	0.0013705933709881018
42-43	0.0	1.370593370988102E-4	0.0	0.33785126594856707	0.0013705933709881018
44-45	0.0	1.370593370988102E-4	0.0	0.40439357411003946	0.0013705933709881018
46-47	0.0	1.370593370988102E-4	0.0	0.4894388927798512	0.0013705933709881018
48-49	0.0	1.370593370988102E-4	0.0	0.5698241939883033	0.0013705933709881018
50-51	0.0	1.370593370988102E-4	0.0	0.650963321550799	0.0013705933709881018
52-53	0.0	1.370593370988102E-4	0.0	0.7290871436971208	0.0013705933709881018
54-55	0.0	1.370593370988102E-4	0.0	0.8052236054555099	0.0013705933709881018
56-57	0.0	1.370593370988102E-4	0.0	0.8863627330180055	0.0013705933709881018
58-59	0.0	1.370593370988102E-4	0.0	0.9790833745653507	0.0013705933709881018
60-61	0.0	1.370593370988102E-4	0.0	1.069474007382016	0.0013705933709881018
62-63	0.0	1.370593370988102E-4	0.0	1.1631540642890528	0.0013705933709881018
64-65	0.0	1.370593370988102E-4	0.0	1.2515573367177852	0.0013705933709881018
66-67	0.0	2.741186741976204E-4	0.0	1.3431129738997902	0.0013705933709881018
68-69	0.0	2.741186741976204E-4	0.0	1.4594763510966802	0.0013705933709881018
70-71	0.0	2.741186741976204E-4	0.0	1.5859135895703327	0.0013705933709881018
72-73	0.0	2.741186741976204E-4	0.0	1.699467250356697	0.0016447120451857223
74-75	0.0	2.741186741976204E-4	0.0	1.8031526388719468	0.0016447120451857223
76-77	0.0	2.741186741976204E-4	0.0	1.9145818799332797	0.0016447120451857223
78-79	0.0	2.741186741976204E-4	0.0	2.0496538566441567	0.0016447120451857223
80-81	0.0	2.741186741976204E-4	0.0	2.1887690837994493	0.0016447120451857223
82-83	0.0	2.741186741976204E-4	0.0	2.338095231568603	0.0016447120451857223
84-85	0.0	2.741186741976204E-4	0.0	2.471385436897196	0.0016447120451857223
86-87	0.0	2.741186741976204E-4	0.0	2.60919860035005	0.0016447120451857223
88-89	0.0	2.741186741976204E-4	0.0	2.763184765580563	0.0016447120451857223
90-91	0.0	2.741186741976204E-4	0.0	2.924092427334566	0.0017817713822845325
92-93	0.0	2.741186741976204E-4	0.0	3.0830812583691856	0.0017817713822845325
94-95	0.0	2.741186741976204E-4	0.0	3.2519383616749202	0.0019188307193833427
96-97	0.0	2.741186741976204E-4	0.0	3.41092719270954	0.0019873603879327476
98-99	0.0	2.741186741976204E-4	0.0	3.5939014077364515	0.0020558900564821526
100-101	0.0	2.741186741976204E-4	0.0	3.7915409718329354	0.0020558900564821526
102-103	0.0	2.741186741976204E-4	0.0	3.9961705621214594	0.002192949393580963
104-105	0.0	2.741186741976204E-4	0.0	4.200046326055939	0.002192949393580963
106-107	0.0	2.741186741976204E-4	0.0	4.406115039384	0.002192949393580963
108-109	0.0	2.741186741976204E-4	0.0	4.619173778904101	0.0023300087306797734
110-111	0.0	2.741186741976204E-4	0.0	4.845321685117138	0.0024670680677785833
112-113	0.0	2.741186741976204E-4	0.0	5.08462728769166	0.0024670680677785833
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCAACG	195	0.0	51.877235	1
GCGCAGA	500	0.0	47.604992	1
GTATCAA	3755	0.0	41.20272	1
GGTATCA	2505	0.0	41.096127	1
ATCAACG	4450	0.0	34.755775	3
CAACGCA	4565	0.0	34.401455	5
TATCAAC	4520	0.0	34.08592	2
TCAACGC	4660	0.0	33.82779	4
AACGCAG	4795	0.0	32.99945	6
ACGCAGA	5180	0.0	30.200201	7
CGCAGAG	5570	0.0	28.083725	8
GCAGAGT	5950	0.0	26.390104	9
GAGTACT	2465	0.0	24.128881	12-13
CAGAGTA	5850	0.0	22.367678	10-11
GTACTTT	2780	0.0	21.28788	14-15
GTATACA	145	0.0038636937	20.519394	1
AGAGTAC	5660	0.0	20.386345	10-11
GTATAGA	235	7.3815754E-6	20.257442	1
GTACATG	5000	0.0	19.875084	1
GGTCTAG	210	7.135581E-5	19.835413	1
>>END_MODULE