##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142457_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	354864
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.795411763379775	33.0	33.0	33.0	27.0	33.0
2	30.883496212633574	33.0	33.0	33.0	27.0	33.0
3	31.015186099463456	33.0	33.0	33.0	27.0	33.0
4	30.959643694485774	33.0	33.0	33.0	27.0	33.0
5	31.0010849226746	33.0	33.0	33.0	27.0	33.0
6	34.22121432436088	37.0	37.0	37.0	27.0	37.0
7	34.18715338834032	37.0	37.0	37.0	27.0	37.0
8	34.18994318950359	37.0	37.0	37.0	27.0	37.0
9	34.17672967672122	37.0	37.0	37.0	27.0	37.0
10-11	34.25615869741648	37.0	37.0	37.0	27.0	37.0
12-13	34.172876087740654	37.0	37.0	37.0	27.0	37.0
14-15	34.16060941656522	37.0	37.0	37.0	27.0	37.0
16-17	34.12385871770594	37.0	37.0	37.0	27.0	37.0
18-19	34.15932019027008	37.0	37.0	37.0	27.0	37.0
20-21	34.1502082487939	37.0	37.0	37.0	27.0	37.0
22-23	34.226555807295185	37.0	37.0	37.0	27.0	37.0
24-25	34.30397842553767	37.0	37.0	37.0	27.0	37.0
26-27	34.337985819919744	37.0	37.0	37.0	27.0	37.0
28-29	34.38539694079985	37.0	37.0	37.0	27.0	37.0
30-31	34.346675064249965	37.0	37.0	37.0	27.0	37.0
32-33	34.365868332657016	37.0	37.0	37.0	27.0	37.0
34-35	34.422487206366384	37.0	37.0	37.0	27.0	37.0
36-37	34.40773648496325	37.0	37.0	37.0	27.0	37.0
38-39	34.38110233779702	37.0	37.0	37.0	27.0	37.0
40-41	34.34426850849903	37.0	37.0	37.0	27.0	37.0
42-43	34.35448791649759	37.0	37.0	37.0	27.0	37.0
44-45	34.32928671265611	37.0	37.0	37.0	27.0	37.0
46-47	34.30225804815366	37.0	37.0	37.0	27.0	37.0
48-49	34.29265155101673	37.0	37.0	37.0	27.0	37.0
50-51	34.223699783579065	37.0	37.0	37.0	27.0	37.0
52-53	34.24562508453943	37.0	37.0	37.0	27.0	37.0
54-55	34.21869223139005	37.0	37.0	37.0	27.0	37.0
56-57	34.174933213850935	37.0	37.0	37.0	27.0	37.0
58-59	34.12578480770098	37.0	37.0	37.0	27.0	37.0
60-61	34.12441949591957	37.0	37.0	37.0	27.0	37.0
62-63	34.090964425808195	37.0	37.0	37.0	27.0	37.0
64-65	34.05788555615672	37.0	37.0	37.0	27.0	37.0
66-67	34.00772549483746	37.0	37.0	37.0	27.0	37.0
68-69	33.9792991117724	37.0	37.0	37.0	27.0	37.0
70-71	33.91269190450426	37.0	37.0	37.0	27.0	37.0
72-73	33.84832640110014	37.0	37.0	37.0	27.0	37.0
74-75	33.76599908697416	37.0	37.0	37.0	24.5	37.0
76-77	33.70759220433744	37.0	37.0	37.0	22.0	37.0
78-79	33.65621759321881	37.0	33.0	37.0	22.0	37.0
80-81	33.59625659407548	37.0	33.0	37.0	22.0	37.0
82-83	33.53539665900176	37.0	33.0	37.0	22.0	37.0
84-85	33.46560795121511	37.0	33.0	37.0	14.0	37.0
86-87	33.38026680643853	37.0	33.0	37.0	14.0	37.0
88-89	33.30993000135263	37.0	33.0	37.0	14.0	37.0
90-91	33.18962053068218	37.0	33.0	37.0	14.0	37.0
92-93	33.105334719779975	37.0	33.0	37.0	14.0	37.0
94-95	33.044991884214795	37.0	33.0	37.0	14.0	37.0
96-97	32.94846194598494	37.0	33.0	37.0	14.0	37.0
98-99	32.82858644438433	37.0	33.0	37.0	14.0	37.0
100-101	32.706421333243156	37.0	33.0	37.0	14.0	37.0
102-103	32.59558309662293	37.0	33.0	37.0	14.0	37.0
104-105	32.443598110825555	37.0	33.0	37.0	14.0	37.0
106-107	32.34370350331395	37.0	33.0	37.0	14.0	37.0
108-109	32.235588845304115	37.0	33.0	37.0	14.0	37.0
110-111	32.15864528382704	37.0	33.0	37.0	14.0	37.0
112-113	31.994407716759095	37.0	33.0	37.0	14.0	37.0
114-115	31.887818995446143	37.0	30.0	37.0	14.0	37.0
116-117	31.679542021732267	37.0	27.0	37.0	14.0	37.0
118-119	31.581611546958833	37.0	27.0	37.0	14.0	37.0
120-121	31.47152852923937	37.0	27.0	37.0	14.0	37.0
122-123	31.39467937012489	37.0	27.0	37.0	14.0	37.0
124-125	29.831357928671267	35.0	24.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	688.0
1	39.0
2	20.0
3	19.0
4	18.0
5	14.0
6	20.0
7	13.0
8	7.0
9	18.0
10	11.0
11	8.0
12	13.0
13	17.0
14	39.0
15	128.0
16	209.0
17	529.0
18	1133.0
19	2215.0
20	3382.0
21	3969.0
22	4371.0
23	4543.0
24	4944.0
25	5615.0
26	6124.0
27	7114.0
28	8450.0
29	9634.0
30	11366.0
31	13285.0
32	16546.0
33	21710.0
34	31077.0
35	54605.0
36	142971.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.1763976947691	20.30374274664245	12.318047174055119	24.20181238453334
2	16.84304897225019	23.298867608629255	36.24698801995543	23.61109539916513
3	19.444947175531073	28.015632423138705	28.22687374160125	24.31254665972898
4	14.268749222434598	19.767691733490167	34.32004026375019	31.643518780325053
5	14.888580293178494	38.11376999940606	31.542001227469292	15.455648479946149
6	29.015072508005414	36.826605393092024	18.40863934792255	15.74968275098001
7	25.784881995687353	33.719474456185374	21.89558646066193	18.600057087465345
8	25.10180723742776	34.535332268867194	20.684461149340834	19.678399344364216
9	25.989989175195088	18.575421757575246	20.127636603102733	35.30695246412694
10-11	24.537239191413896	27.761267849446412	25.974238859331173	21.727254099808523
12-13	25.33587406418927	26.73973152692647	25.4115022730871	22.512892135797163
14-15	24.04322663860697	26.85782333706708	23.66419447178756	25.434755552538384
16-17	23.439759879317585	28.75990900749458	24.39563360388853	23.404697509299304
18-19	23.53435919472802	27.817420042463088	25.929480746015905	22.71874001679299
20-21	25.19123350558012	26.722938974886645	25.26727461866371	22.818552900869527
22-23	25.18383262221707	26.443914620823577	25.00335654413043	23.368896212828925
24-25	24.149868938864042	27.30254279799628	25.180270883231948	23.367317379907725
26-27	23.84547119471828	27.164211585421	25.24037374513883	23.749943474721896
28-29	23.665908352291193	26.903533933390793	25.82258269630216	23.607975018015853
30-31	24.095432154938774	26.792814892480564	25.679750623864255	23.432002328716408
32-33	23.527441876238143	27.277261718716883	25.44714390276298	23.748152502281993
34-35	23.839772136446864	27.006380405542874	25.607805683025518	23.54604177498474
36-37	23.89663235678509	27.200695145987723	25.2212245589991	23.681447938228082
38-39	23.71031774063661	27.078877099786663	25.48197962730111	23.72882553227561
40-41	23.515215910792854	27.05250188626993	25.802335866257103	23.629946336680106
42-43	23.68731880167508	27.103878562507415	25.47909826106122	23.729704374756285
44-45	23.69767274299301	27.11657759148043	25.43043588211277	23.75531378341379
46-47	23.721287690314632	27.07246401381101	25.436644670201357	23.769603625672996
48-49	23.781250132438263	27.212213421258213	25.430830497388673	23.575705948914855
50-51	23.35235275254177	27.509313111250183	25.457673904782357	23.680660231425694
52-53	23.34387219999548	27.22630026446057	25.419718134761872	24.010109400782078
54-55	23.42838978561085	27.084660756542934	25.393282237841277	24.093667220004946
56-57	23.6935525566717	26.80193193039061	25.225139957592464	24.27937555534523
58-59	23.53855194313546	26.940310314043238	25.58692409397566	23.934213648845645
60-61	23.565871325336605	27.094904203086067	25.640435281288664	23.698789190288664
62-63	23.13637968783106	27.10784659933611	25.629917367045696	24.12585634578713
64-65	23.033288086658917	26.87723084702622	25.714471355465125	24.37500971084974
66-67	23.650213852681777	26.72296896452362	25.39578283641541	24.231034346379193
68-69	23.505663751566203	26.585518481529768	25.358266365127278	24.550551401776747
70-71	23.4115824073067	26.681008485026545	25.522033242319758	24.385375865346994
72-73	23.654917691259243	26.603484438669216	25.676654825884	24.064943044187544
74-75	23.560273028286	26.573218140107812	25.80419600176293	24.062312829843258
76-77	23.40926655655572	26.40077087080426	25.8464955472886	24.34346702535142
78-79	23.461654190569963	26.567799662940555	25.673306615321373	24.29723953116811
80-81	23.20886951288597	26.692735741238067	25.826466420762213	24.27192832511376
82-83	23.5929311051152	26.437582110214862	25.888061704502114	24.081425080167822
84-85	23.50390041165518	26.65728646623552	25.66403060431659	24.174782517792714
86-87	23.19673543644751	26.641109883807978	25.784571575471137	24.37758310427337
88-89	23.56505367763477	26.161297020012064	25.907491705777918	24.366157596575253
90-91	23.523106341992502	26.380434813310472	25.8119914020286	24.284467442668422
92-93	23.466604901045542	26.534030644598637	25.882808207860148	24.116556246495673
94-95	22.72209237461075	26.64924904144218	26.30946412557637	24.3191944583707
96-97	23.19684585708072	26.500533786722553	25.8475629388207	24.45505741737603
98-99	23.072480222781437	26.858183009526392	25.706147671781487	24.363189095910684
100-101	23.22433614698382	26.981957430946014	25.47280906618621	24.320897355883954
102-103	23.16100572448481	27.064076386711967	25.815110719500247	23.95980716930298
104-105	23.034646369791925	27.25039009821296	25.744646299185913	23.9703172328092
106-107	23.03931951233977	27.1943721953465	25.717101714492273	24.049206577821458
108-109	23.03578867103935	27.30698899298912	25.65883208483659	23.998390251134943
110-111	22.86045513338266	27.660310539009416	25.573011686162435	23.906222641445492
112-113	22.86883058383967	27.772310012411904	25.317746201236673	24.041113202511752
114-115	22.76619505934741	27.829961583369457	25.406367757702743	23.997475599580394
116-117	22.896072631259397	27.66144005873757	25.335940754128224	24.106546555874818
118-119	22.780626507112725	27.474347317833487	25.51870090412645	24.226325270927337
120-121	22.699427212826368	27.58174253237723	25.727561129535744	23.99126912526066
122-123	22.711564360796537	27.74907321921154	25.752506472642672	23.786855947349252
124-125	22.85370219782702	27.691660454925348	25.383998554358385	24.070638792889252
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1068.0
1	614.0
2	144.5
3	111.0
4	92.0
5	69.5
6	53.0
7	63.0
8	64.0
9	62.0
10	60.0
11	56.5
12	54.0
13	55.0
14	68.0
15	83.0
16	88.5
17	106.0
18	134.0
19	152.5
20	184.0
21	243.5
22	308.5
23	393.0
24	473.5
25	598.0
26	831.0
27	1073.0
28	1353.5
29	1875.5
30	2499.5
31	3135.5
32	3917.0
33	4724.0
34	5574.0
35	6370.5
36	7192.0
37	8239.5
38	9011.5
39	9310.0
40	9359.0
41	9392.5
42	9732.0
43	10446.0
44	11382.0
45	12354.5
46	13403.5
47	14370.0
48	15316.5
49	15847.5
50	16267.5
51	15920.0
52	14692.5
53	14160.5
54	13623.0
55	12724.0
56	11817.0
57	10391.5
58	9290.0
59	8410.5
60	7178.5
61	6085.0
62	4923.0
63	3832.5
64	2925.5
65	2228.5
66	1704.0
67	1312.5
68	1044.5
69	860.0
70	730.5
71	649.0
72	534.0
73	466.5
74	390.5
75	309.5
76	238.0
77	176.5
78	134.0
79	88.0
80	68.5
81	46.0
82	26.0
83	16.0
84	11.0
85	7.0
86	2.0
87	1.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.39648992290004054
2	0.3595743721538392
3	0.34942964065106635
4	0.33703052436989944
5	0.3638013436133279
6	0.29335181928851617
7	0.28771585734253124
8	0.2843342801749403
9	0.2950426078723116
10-11	0.29208372785066955
12-13	0.3268857928671266
14-15	0.37535506560259707
16-17	0.34055300058614
18-19	0.32265882140763785
20-21	0.3122322918075657
22-23	0.3036374498399387
24-25	0.28785675639118086
26-27	0.29307002119121695
28-29	0.2843342801749403
30-31	0.28771585734253124
32-33	0.2837706839803418
34-35	0.27278055818567115
36-37	0.27658483249921095
38-39	0.2702443753099779
40-41	0.2792619144235538
42-43	0.2733441543802696
44-45	0.26756729338563506
46-47	0.26587650480183955
48-49	0.26080413905045313
50-51	0.26136773524505164
52-53	0.2637630190720952
54-55	0.26813088958023357
56-57	0.2585497542720592
58-59	0.2536182875693223
60-61	0.24967311420713284
62-63	0.2505185084990306
64-65	0.2475596284773885
66-67	0.2485459218179359
68-69	0.2543227828125705
70-71	0.25009581135308173
72-73	0.24276906082330132
74-75	0.2558726723477163
76-77	0.2757394382073132
78-79	0.2593951485639569
80-81	0.26277672573154787
82-83	0.258972451418008
84-85	0.261226836196402
86-87	0.24826412372063664
88-89	0.2402328779476081
90-91	0.2330470264664773
92-93	0.2360059064881194
94-95	0.2300881464448352
96-97	0.22262049686640517
98-99	0.22402948735290137
100-101	0.21332115965553003
102-103	0.21726633301771947
104-105	0.22163420352585778
106-107	0.215575544433924
108-109	0.21740723206636908
110-111	0.21698453492042022
112-113	0.21656183777447133
114-115	0.20303552910410752
116-117	0.21036227963388793
118-119	0.20007664908246542
120-121	0.20247193290950902
122-123	0.19190450426078723
124-125	0.19613147572027592
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	354864.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.03453795823553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.73331940191946	68.35530261627405
2	7.763759872645863	11.961553092159273
3	1.590007716340094	3.674565293348652
4	0.6178952596104721	1.9039710332270687
5	0.30425087145630175	1.171891265301336
6	0.18525257250832072	0.856250779725181
7	0.1260097161422783	0.6794970182888176
8	0.08967191137221647	0.5526267408312435
9	0.07485330505171331	0.5189660792375034
>10	0.47368291900317044	6.988802820672195
>50	0.029555305278109886	1.5082883052862368
>100	0.010931414280832356	1.3932017906656373
>500	8.097343911727671E-4	0.43508316498288874
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	780	0.21980251589341268	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	615	0.17330582983903692	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	495	0.1394900581631273	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	389	0.10961945984940709	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	2.8179809729924704E-4	0.0
16-17	0.0	0.0	0.0	4.226971459488706E-4	0.0
18-19	0.0	0.0	0.0	5.635961945984941E-4	0.0
20-21	0.0	0.0	0.0	7.044952432481176E-4	0.0
22-23	0.0	0.0	0.0	0.002677081924342847	0.0
24-25	0.0	0.0	0.0	0.008172144821678164	0.0
26-27	0.0	0.0	0.0	0.013949005816312728	0.0
28-29	0.0	0.0	0.0	0.02648902114612922	0.0
30-31	0.0	0.0	0.0	0.051428152757112586	0.0
32-33	0.0	0.0	0.0	0.08397583299517561	0.0
34-35	0.0	0.0	0.0	0.12680914378466115	0.0
36-37	0.0	0.0	0.0	0.1838732584877587	0.0
38-39	0.0	0.0	0.0	0.27165336579647414	0.0
40-41	0.0	0.0	0.0	0.3660557283917219	0.0
42-43	0.0	0.0	0.0	0.4500315613868975	0.0
44-45	0.0	0.0	0.0	0.526962441949592	0.0
46-47	0.0	0.0	0.0	0.6154470445015555	0.0
48-49	0.0	0.0	0.0	0.7029453537129717	0.0
50-51	0.0	0.0	0.0	0.7943888362865774	0.0
52-53	0.0	0.0	0.0	0.8825916407412417	0.0
54-55	0.0	0.0	0.0	0.9699490509040083	0.0
56-57	0.0	0.0	0.0	1.0653377068398036	0.0
58-59	0.0	0.0	0.0	1.177352450516254	0.0
60-61	0.0	0.0	0.0	1.2841539293926687	0.0
62-63	0.0	0.0	0.0	1.3826423643987558	0.0
64-65	0.0	0.0	0.0	1.4792991117723973	0.0
66-67	0.0	0.0	0.0	1.5917365525947968	0.0
68-69	0.0	0.0	0.0	1.7093872582172325	0.0
70-71	0.0	0.0	0.0	1.8363373010505433	0.0
72-73	0.0	0.0	0.0	1.9489156409215926	0.0
74-75	0.0	0.0	0.0	2.0643119617656343	0.0
76-77	0.0	0.0	0.0	2.1873168312367555	0.0
78-79	0.0	0.0	0.0	2.3230026150863425	0.0
80-81	0.0	0.0	0.0	2.474046395238739	0.0
82-83	0.0	0.0	0.0	2.6221312953694937	0.0
84-85	0.0	0.0	0.0	2.7679618107218538	0.0
86-87	0.0	0.0	0.0	2.910128950809324	0.0
88-89	0.0	0.0	0.0	3.065822399567158	0.0
90-91	0.0	0.0	0.0	3.2351830560440056	0.0
92-93	0.0	0.0	0.0	3.4142657468776774	0.0
94-95	0.0	0.0	0.0	3.5809493214301815	0.0
96-97	0.0	0.0	0.0	3.7576367284368093	0.0
98-99	0.0	0.0	0.0	3.939255602146174	0.0
100-101	0.0	0.0	0.0	4.145954506515173	0.0
102-103	0.0	0.0	0.0	4.3540624013706655	0.0
104-105	0.0	0.0	0.0	4.557379728572073	0.0
106-107	0.0	0.0	0.0	4.765487623427567	0.0
108-109	0.0	0.0	0.0	4.979795076423644	0.0
110-111	0.0	0.0	0.0	5.2114331124036255	0.0
112-113	0.0	0.0	0.0	5.4529340817890795	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTAAG	30	0.004211246	59.581482	1
TCTAACG	85	0.0071755513	28.022488	2
GGTATCA	1790	0.0	27.627167	1
CTAACGC	90	0.00948303	26.465685	3
TATTCTC	215	1.2175042E-7	24.92698	5
GTATCAA	2295	0.0	23.365288	1
CATGGGG	1430	0.0	23.319412	4
TCTACAC	155	2.0908604E-4	23.050756	3
TGGGGGG	475	0.0	22.559097	6
GTACATG	3450	0.0	20.896694	1
ATCAACG	2505	0.0	20.44355	3
GTACAAA	350	1.4988473E-9	20.427937	1
ATGGGGG	875	0.0	20.416384	5
TATCAAC	2550	0.0	20.316303	2
ACATGGG	3300	0.0	20.210157	3
CAACGCA	2510	0.0	20.165585	5
CATGGGT	415	1.8189894E-12	20.08841	4
AACGAAT	90	5.774125E-4	19.836643	30-31
TACATGG	3535	0.0	19.708887	2
TCAACGC	2600	0.0	19.696577	4
>>END_MODULE