FastQCFastQC Report
Sun 1 Jan 2017
ERR1142457_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142457_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354864
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17050.4804657558952162No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16250.45792190811127637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6330.17837819559042337No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6110.17217863744983994No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.1270909418819604No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4160.11722800847648676No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG1250.057.1059271
CGTCATA350.007808124650.973072
GACTTAT505.334064E-447.5882721
GCGCAGA1900.046.9621121
GGTTAGG700.00278976833.9916271
GTCCTAT1606.9667294E-933.4605061
CGTACTT953.2919555E-431.2992522
GTATCAA28900.031.2864071
GTGTTAT1602.5999907E-729.742671
GGTATCA21400.029.1867331
ATCAACG32650.026.774523
TATCAAC33600.026.5484732
CAACGCA33700.026.4696945
ACATACC900.00954480326.430484
AACGCAG35100.025.9222016
GCGTACT1150.001004884125.8631921
GTAAGTA1150.001006538625.8559042
TCAACGC34950.025.6931484
TACAGCG1200.001290074324.7785747
GCGTAGC600.001291346324.77857420-21
Produced by FastQC (version 0.11.5)