##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142457_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	354864
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.581163487984128	27.0	14.0	33.0	14.0	33.0
2	26.10635341990171	27.0	27.0	33.0	14.0	33.0
3	25.28739460751161	27.0	14.0	33.0	14.0	33.0
4	30.56538279453537	33.0	27.0	33.0	27.0	33.0
5	32.11171885567428	33.0	33.0	33.0	33.0	33.0
6	35.73239043689977	37.0	37.0	37.0	33.0	37.0
7	35.76963287343884	37.0	37.0	37.0	33.0	37.0
8	36.08931308895802	37.0	37.0	37.0	37.0	37.0
9	36.23422212453222	37.0	37.0	37.0	37.0	37.0
10-11	36.206177577888994	37.0	37.0	37.0	37.0	37.0
12-13	36.252433326570184	37.0	37.0	37.0	37.0	37.0
14-15	36.24593365345597	37.0	37.0	37.0	37.0	37.0
16-17	36.242433721087515	37.0	37.0	37.0	37.0	37.0
18-19	36.2360284503359	37.0	37.0	37.0	37.0	37.0
20-21	36.24735673384733	37.0	37.0	37.0	37.0	37.0
22-23	36.24735532485685	37.0	37.0	37.0	37.0	37.0
24-25	36.228145148563954	37.0	37.0	37.0	37.0	37.0
26-27	36.188975213039356	37.0	37.0	37.0	37.0	37.0
28-29	36.1622184837008	37.0	37.0	37.0	37.0	37.0
30-31	36.107476385319444	37.0	37.0	37.0	37.0	37.0
32-33	36.06159120113621	37.0	37.0	37.0	37.0	37.0
34-35	35.98328655484919	37.0	37.0	37.0	37.0	37.0
36-37	35.90538065286983	37.0	37.0	37.0	37.0	37.0
38-39	35.8515853960954	37.0	37.0	37.0	35.0	37.0
40-41	35.7190416610307	37.0	37.0	37.0	33.0	37.0
42-43	35.678104851436046	37.0	37.0	37.0	33.0	37.0
44-45	35.611483554263046	37.0	37.0	37.0	33.0	37.0
46-47	35.477051771946435	37.0	37.0	37.0	33.0	37.0
48-49	35.337670206050774	37.0	37.0	37.0	33.0	37.0
50-51	35.253560518959375	37.0	37.0	37.0	33.0	37.0
52-53	35.18970084313991	37.0	37.0	37.0	33.0	37.0
54-55	35.14344509445872	37.0	37.0	37.0	33.0	37.0
56-57	35.09514490058163	37.0	37.0	37.0	33.0	37.0
58-59	35.05225100320123	37.0	37.0	37.0	33.0	37.0
60-61	35.02416277785292	37.0	37.0	37.0	33.0	37.0
62-63	35.01750247982326	37.0	37.0	37.0	33.0	37.0
64-65	34.99406815005185	37.0	37.0	37.0	30.0	37.0
66-67	34.964432853149376	37.0	37.0	37.0	27.0	37.0
68-69	34.91945646782993	37.0	37.0	37.0	27.0	37.0
70-71	34.88925616574237	37.0	37.0	37.0	27.0	37.0
72-73	34.876028563055144	37.0	37.0	37.0	27.0	37.0
74-75	34.778274212092526	37.0	37.0	37.0	27.0	37.0
76-77	34.738126437170294	37.0	37.0	37.0	27.0	37.0
78-79	34.710001578069345	37.0	37.0	37.0	27.0	37.0
80-81	34.68928800892736	37.0	37.0	37.0	27.0	37.0
82-83	34.63812051941025	37.0	37.0	37.0	27.0	37.0
84-85	34.559264957843006	37.0	37.0	37.0	27.0	37.0
86-87	34.404585982235446	37.0	37.0	37.0	27.0	37.0
88-89	34.32373388114884	37.0	37.0	37.0	27.0	37.0
90-91	34.295191960863875	37.0	37.0	37.0	27.0	37.0
92-93	34.23609044591731	37.0	37.0	37.0	27.0	37.0
94-95	34.26068719284008	37.0	37.0	37.0	27.0	37.0
96-97	34.22716167320438	37.0	37.0	37.0	27.0	37.0
98-99	34.17100917534605	37.0	37.0	37.0	27.0	37.0
100-101	34.124828103160645	37.0	37.0	37.0	27.0	37.0
102-103	34.00981361873845	37.0	37.0	37.0	22.0	37.0
104-105	33.93676168898507	37.0	37.0	37.0	22.0	37.0
106-107	33.866114905992156	37.0	37.0	37.0	22.0	37.0
108-109	33.76573842373416	37.0	37.0	37.0	22.0	37.0
110-111	33.70357517246043	37.0	33.0	37.0	22.0	37.0
112-113	33.6460982235448	37.0	33.0	37.0	22.0	37.0
114-115	33.534752750349426	37.0	33.0	37.0	22.0	37.0
116-117	33.4277131520808	37.0	33.0	37.0	22.0	37.0
118-119	33.291004441138014	37.0	33.0	37.0	14.0	37.0
120-121	33.20688489111322	37.0	33.0	37.0	14.0	37.0
122-123	33.073667658595966	37.0	33.0	37.0	14.0	37.0
124-125	31.85199823030795	37.0	30.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	143.0
16	65.0
17	25.0
18	49.0
19	211.0
20	838.0
21	2209.0
22	3394.0
23	4310.0
24	3674.0
25	2598.0
26	2465.0
27	2943.0
28	3477.0
29	4653.0
30	6226.0
31	8484.0
32	12195.0
33	18427.0
34	30824.0
35	70248.0
36	177402.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.646931481210046	12.765387694354558	11.915526941854303	21.672153882581096
2	18.13624374408224	20.2846724378917	36.12736146805537	25.451722349970694
3	21.903444155756322	24.997041103302127	28.848397405188496	24.251117335753055
4	14.957561206546732	17.756661707020154	35.54234861806213	31.743428468370983
5	15.513485637957611	35.67783260393565	32.290289946147624	16.518391811959116
6	29.314941103533787	34.919404835709855	18.788254522910446	16.977399537845912
7	25.592713561466645	32.38739640017923	23.334019067157765	18.685870971196362
8	25.673497452545202	31.87728256458812	21.644630055457867	20.80458992740881
9	26.618930068984177	16.013176879029714	21.428490914829343	35.93940213715677
10-11	25.190777311871592	25.267285495288338	27.098973127733437	22.442964065106633
12-13	26.36830781413135	24.373597171870312	27.037438338966957	22.220656675031385
14-15	24.680018260516707	24.399488254655306	25.82792280986519	25.092570674962804
16-17	23.160140222733215	26.607235447946252	26.865644303169667	23.366980026150863
18-19	22.95146873168312	25.298424185039902	29.304747734343294	22.44535934893368
20-21	24.33865415857083	24.356830161456447	29.089213176334	22.215302503638725
22-23	24.186342072374305	24.329213909010083	28.798960727152835	22.685483291462774
24-25	23.295685107534155	25.068899634789666	28.979834528157266	22.655580729518913
26-27	22.976689661391404	25.010003832454125	28.963490238513913	23.049816267640562
28-29	22.80746990396321	24.908415618377745	29.511587537760946	22.772526939898103
30-31	23.21819063077686	24.721442580819694	29.450860047792958	22.609506740610488
32-33	22.867211100590648	24.987600883718834	29.098612426168895	23.04657558952162
34-35	22.933715451553272	24.813872356733846	29.2104862707967	23.04192592091618
36-37	23.210473886438024	24.858431481400594	28.759227139449393	23.171867492711986
38-39	23.098003742278735	24.957307588259166	28.880359799810634	23.064328869651472
40-41	22.869514124803263	24.93782663035131	29.01921773571985	23.173441509125574
42-43	23.232378738243685	24.873295994927613	28.737327838247207	23.156997428581494
44-45	23.10631678614906	25.00746764957843	28.433005320348077	23.45321024392443
46-47	23.16599759613691	24.96678131750297	28.318662629228776	23.548558457131342
48-49	23.329420766290113	25.196321153648338	28.13116916239596	23.343088917665593
50-51	23.06066549438658	25.439182334640876	27.929573019522973	23.570579151449568
52-53	22.936110735380314	25.54556111637134	27.732455250462152	23.785872897786195
54-55	23.118997700527526	25.454822129040984	27.542805130979758	23.883375039451735
56-57	23.31160670003156	25.21768903016367	27.42374543487082	24.046958834933946
58-59	23.085886424094866	25.309273411785927	27.782051715586814	23.822788448532396
60-61	23.02868141034312	25.396067225754095	28.06300441859417	23.512246945308625
62-63	22.707290680373326	25.478352270165473	27.98607353803147	23.82828351142973
64-65	22.72039429189774	25.209235087244693	28.00495401055052	24.065416610307047
66-67	23.033620786833502	25.3171852102356	27.865798669823015	23.78339533310788
68-69	23.065878759186617	25.10017922358988	28.02355268497227	23.810389332251226
70-71	22.79957955723883	25.2052899138825	28.07723522250778	23.91789530637089
72-73	23.063060778213625	25.147803102033457	28.38664953334235	23.402486586410568
74-75	23.026201680032376	25.13604257473352	28.319840711383865	23.517915033850244
76-77	22.913979722291526	25.208436406281493	28.279411205033767	23.598172666393218
78-79	22.989435359561295	25.215787501091967	28.28838171350634	23.506395425840395
80-81	22.617819784480815	25.33026737003472	28.374109518012535	23.67780332747193
82-83	23.001097605135495	25.07372553108449	28.53562003418216	23.38955682959786
84-85	22.874697412515737	25.329515479367657	28.109161298690676	23.686625809425927
86-87	22.649089289067938	25.44003456733641	28.154587161792925	23.756288981802726
88-89	23.104117467599234	24.997740138529508	28.005898238437982	23.892244155433275
90-91	23.15579004107573	25.100078877818397	27.96028170246271	23.78384937864316
92-93	22.956876243140684	25.32367504126169	27.971758664955072	23.747690050642554
94-95	22.401039274311497	25.199724964281987	28.22737225351755	24.17186350788896
96-97	22.70996776229767	25.129627124757654	27.902661301230896	24.257743811713784
98-99	22.705458992740883	25.402689481040625	27.561967401596103	24.329884124622392
100-101	22.880314712115062	25.4679257405654	27.38697078317327	24.264788764146264
102-103	22.830718246990397	25.379441138013437	27.632135127823616	24.15770548717255
104-105	22.86757754103373	25.537359046723996	27.40879866877266	24.186264743469618
106-107	22.822062031145204	25.489074379885814	27.370834202235283	24.3180293867337
108-109	22.887364627042746	25.62075000070452	27.147297613377937	24.344587758874802
110-111	22.66726220333538	25.914017279956713	27.124065129543318	24.29465538716459
112-113	22.663893773389244	25.933878894449702	26.801535236034084	24.600692096126966
114-115	22.772840824711473	26.000842577498105	26.953039689908938	24.27327690788148
116-117	22.856361873844627	25.909080661887373	26.843523152531674	24.391034311736327
118-119	22.68390143829749	25.602625231074438	27.142229135668877	24.571244194959196
120-121	22.703114740701288	25.546340679871836	27.410450852587353	24.340093726839523
122-123	22.53370305243699	26.033917218990936	27.30172685874025	24.130652869831824
124-125	22.51538617611254	25.841590017584203	27.20802899138825	24.434994814915008
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	54.0
1	58.5
2	150.5
3	229.0
4	190.5
5	190.5
6	261.0
7	409.0
8	659.5
9	849.0
10	805.0
11	671.5
12	625.0
13	613.0
14	608.5
15	616.5
16	628.0
17	644.0
18	640.0
19	645.5
20	630.0
21	610.0
22	646.5
23	690.5
24	714.0
25	781.5
26	930.5
27	1094.0
28	1273.5
29	1610.5
30	2199.0
31	2826.5
32	3515.0
33	4309.0
34	5116.0
35	5897.5
36	6619.0
37	7495.5
38	8169.0
39	8611.5
40	8851.0
41	8851.5
42	9121.5
43	9959.5
44	10935.0
45	11906.0
46	12965.5
47	13796.5
48	14800.0
49	15670.0
50	16172.5
51	15515.0
52	14281.0
53	13970.5
54	13752.0
55	13047.0
56	12020.0
57	10677.0
58	9644.0
59	8787.0
60	7648.5
61	6295.0
62	4976.0
63	3927.5
64	2925.0
65	2279.5
66	1776.0
67	1301.5
68	1003.0
69	832.0
70	742.5
71	620.0
72	528.0
73	505.0
74	402.0
75	311.5
76	251.0
77	164.0
78	120.5
79	89.0
80	60.0
81	42.0
82	28.0
83	18.0
84	11.5
85	8.5
86	6.5
87	2.5
88	1.0
89	1.5
90	1.0
91	1.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028179809729924704
2	0.0
3	5.63596194598494E-4
4	0.0
5	0.0014089904864962352
6	0.001127192389196988
7	0.0036633752648902113
8	0.0
9	0.0
10-11	0.0
12-13	1.408990486496235E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.408990486496235E-4
22-23	1.408990486496235E-4
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	1.408990486496235E-4
38-39	0.0
40-41	0.002677081924342847
42-43	4.2269714594887054E-4
44-45	0.0
46-47	0.00521326480003607
48-49	0.006904053383831553
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.006763154335181929
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.08101695297353352
76-77	0.03931083457324496
78-79	2.81798097299247E-4
80-81	0.0
82-83	1.408990486496235E-4
84-85	0.060445691870688496
86-87	0.21768903016366833
88-89	0.24234636367735243
90-91	0.14611231344965958
92-93	0.11807340276838452
94-95	2.81798097299247E-4
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	9.862933405473645E-4
106-107	0.0028179809729924703
108-109	0.0030997790702917173
110-111	5.63596194598494E-4
112-113	0.0
114-115	1.408990486496235E-4
116-117	0.0
118-119	0.0
120-121	8.453942918977411E-4
122-123	0.0
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	354864.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.68701697767655
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.32104016724807	64.34426969312385
2	8.688896449533695	12.80517720388126
3	1.8241779928229191	4.032547041823391
4	0.6635505232889358	1.955802347005519
5	0.3502738346153674	1.2905316999069225
6	0.2135307594022554	0.9440666815998098
7	0.15387969736982016	0.7937255110826769
8	0.1066483807877578	0.6286880836599378
9	0.07847084776532573	0.5204054422382607
>10	0.5448246813593906	7.552175327080943
>50	0.0403987685955679	2.0217873075937294
>100	0.012624615186085746	1.6924716449120927
>500	8.416410124057164E-4	0.38575205684694824
>1k	8.416410124057164E-4	1.0325999592446444
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1705	0.4804657558952162	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1625	0.45792190811127637	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	633	0.17837819559042337	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	611	0.17217863744983994	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	451	0.1270909418819604	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	416	0.11722800847648676	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	2.8179809729924704E-4	0.0
16-17	0.0	0.0	0.0	4.226971459488706E-4	2.8179809729924704E-4
18-19	0.0	0.0	0.0	5.635961945984941E-4	2.8179809729924704E-4
20-21	0.0	0.0	0.0	7.044952432481176E-4	2.8179809729924704E-4
22-23	0.0	0.0	0.0	0.002677081924342847	2.8179809729924704E-4
24-25	0.0	0.0	0.0	0.008172144821678164	2.8179809729924704E-4
26-27	0.0	0.0	0.0	0.013949005816312728	2.8179809729924704E-4
28-29	0.0	0.0	0.0	0.02648902114612922	2.8179809729924704E-4
30-31	0.0	0.0	0.0	0.051428152757112586	2.8179809729924704E-4
32-33	0.0	0.0	0.0	0.08397583299517561	2.8179809729924704E-4
34-35	0.0	0.0	0.0	0.12680914378466115	2.8179809729924704E-4
36-37	0.0	0.0	0.0	0.18373235943910907	2.8179809729924704E-4
38-39	0.0	0.0	0.0	0.27165336579647414	2.8179809729924704E-4
40-41	0.0	0.0	0.0	0.3663375264890212	2.8179809729924704E-4
42-43	0.0	0.0	0.0	0.4511587537760945	2.8179809729924704E-4
44-45	0.0	0.0	0.0	0.5293577257766355	2.8179809729924704E-4
46-47	0.0	0.0	0.0	0.6179832273772488	2.8179809729924704E-4
48-49	0.0	0.0	0.0	0.7059042337346138	2.8179809729924704E-4
50-51	0.0	0.0	0.0	0.797911312502818	2.8179809729924704E-4
52-53	0.0	0.0	0.0	0.8882276026872267	2.8179809729924704E-4
54-55	0.0	8.453942918977412E-4	0.0	0.976430407141891	2.8179809729924704E-4
56-57	0.0	0.0011271923891969882	0.0	1.0728053564182334	2.8179809729924704E-4
58-59	0.0	0.0012680914378466117	0.0	1.1856654943865819	2.8179809729924704E-4
60-61	0.0	0.0014089904864962352	0.0	1.2930305694575952	2.8179809729924704E-4
62-63	0.0	0.0014089904864962352	0.0	1.3913781054150323	2.8179809729924704E-4
64-65	0.0	0.0014089904864962352	0.0	1.4877530546913746	2.8179809729924704E-4
66-67	0.0	0.0014089904864962352	0.0	1.6000495964651247	2.8179809729924704E-4
68-69	0.0	0.0014089904864962352	0.0	1.7188274944767574	2.8179809729924704E-4
70-71	0.0	0.0014089904864962352	0.0	1.8466229316019658	2.8179809729924704E-4
72-73	0.0	0.0014089904864962352	0.0	1.959905766716263	5.635961945984941E-4
74-75	0.0	0.0014089904864962352	0.0	2.075161188511655	8.453942918977411E-4
76-77	0.0	0.0014089904864962352	0.0	2.198447856080076	0.0011271923891969882
78-79	0.0	0.0014089904864962352	0.0	2.33526083231886	0.0014089904864962352
80-81	0.0	0.0014089904864962352	0.0	2.4878545020064022	0.0014089904864962352
82-83	0.0	0.0014089904864962352	0.0	2.636502998331755	0.0016907885837954822
84-85	0.0	0.0014089904864962352	0.0	2.7823335136841156	0.0016907885837954822
86-87	0.0	0.0014089904864962352	0.0	2.9270368366472788	0.0016907885837954822
88-89	0.0	0.0014089904864962352	0.0	3.082448487307814	0.0016907885837954822
90-91	0.0	0.0014089904864962352	0.0	3.254908922854953	0.0016907885837954822
92-93	0.0	0.0014089904864962352	0.0	3.4351188060778215	0.0016907885837954822
94-95	0.0	0.0014089904864962352	0.0	3.6049021597006177	0.0016907885837954822
96-97	0.0	0.0014089904864962352	0.0	3.7831394562423917	0.0016907885837954822
98-99	0.0	0.0014089904864962352	0.0	3.967717209973398	0.0016907885837954822
100-101	0.0	0.0014089904864962352	0.0	4.178079489607287	0.0016907885837954822
102-103	0.0	0.0014089904864962352	0.0	4.392105144506065	0.0016907885837954822
104-105	0.0	0.0014089904864962352	0.0	4.603594616529149	0.0016907885837954822
106-107	0.0	0.0014089904864962352	0.0	4.818042968573876	0.0016907885837954822
108-109	0.0	0.0014089904864962352	0.0	5.036295594932143	0.0016907885837954822
110-111	0.0	0.0014089904864962352	0.0	5.272019703322963	0.0016907885837954822
112-113	0.0	0.0014089904864962352	0.0	5.520706524189549	0.0016907885837954822
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCAACG	125	0.0	57.105927	1
CGTCATA	35	0.0078081246	50.97307	2
GACTTAT	50	5.334064E-4	47.588272	1
GCGCAGA	190	0.0	46.962112	1
GGTTAGG	70	0.002789768	33.991627	1
GTCCTAT	160	6.9667294E-9	33.460506	1
CGTACTT	95	3.2919555E-4	31.299252	2
GTATCAA	2890	0.0	31.286407	1
GTGTTAT	160	2.5999907E-7	29.74267	1
GGTATCA	2140	0.0	29.186733	1
ATCAACG	3265	0.0	26.77452	3
TATCAAC	3360	0.0	26.548473	2
CAACGCA	3370	0.0	26.469694	5
ACATACC	90	0.009544803	26.43048	4
AACGCAG	3510	0.0	25.922201	6
GCGTACT	115	0.0010048841	25.863192	1
GTAAGTA	115	0.0010065386	25.855904	2
TCAACGC	3495	0.0	25.693148	4
TACAGCG	120	0.0012900743	24.778574	7
GCGTAGC	60	0.0012913463	24.778574	20-21
>>END_MODULE