FastQCFastQC Report
Sun 1 Jan 2017
ERR1142456_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142456_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266595
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5730.21493276318010468No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4240.15904274273711058No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3720.13953750070331403No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3660.13728689585326057No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACC558.4841566E-443.3062489
AGAGGCG800.005349387429.756255
GCATAGC900.00948019326.4649281
CATGGGT3553.6379788E-1223.4697194
TAAGGTG1450.003854988120.5215534
TCAAGAC1500.004690861319.83753
ATGGGGG5700.019.3154618-19
GCTTGTA1550.00565157319.2084161
TACTGAA1550.005663875519.201197
GTTCCGA1550.00567003519.197586
GGTATCA18800.018.6873361
ATGGGTA1600.001759148218.5976565
CGCGCGC800.00683078618.59241344-45
TACAAAA2902.1737196E-618.4693982
CATGGGG10400.018.311544
ATGGGTG1959.86717E-418.3115395
TATTCTC1959.86717E-418.3115395
GTACAAA2000.001165043117.8638271
GACCTAC1700.009628355517.5135561
CTTAATC1700.00965969217.5036753
Produced by FastQC (version 0.11.5)