FastQCFastQC Report
Sun 1 Jan 2017
ERR1142456_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142456_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266595
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12510.4692511112361447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11850.4444944578855568No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5320.19955363003807272No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5120.1920516138712279No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4240.15904274273711058No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3500.1312852829197847No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3060.11478084735272605No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAGA1550.057.5603141
GCGTACA350.007800606550.981991
GTCAACG751.4701072E-647.583191
CTATTGA1802.1926098E-829.7394969
GGGCTAT1602.5983172E-729.7394946
CACTTAA1055.9058424E-428.3233287
GTATCAA24650.027.9901141
GGTATCA18900.027.6939221
GCTTTAG1306.3755084E-527.4518411
ACTTTAT1107.751512E-427.0359068
GGATAAG1150.001004852525.8604321
GATAACC1651.1087008E-525.233512
ATCAACG27750.024.8632893
GGTTTCA1200.001287917124.7829131
CAACGCA28700.024.6620225
GGCTATT1951.4622565E-624.4016387
TATCAAC28850.024.1214622
TCAACGC29600.023.912164
AACGCAG29700.023.8316486
TTTAGGG2001.8226856E-623.7915972
Produced by FastQC (version 0.11.5)