##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142456_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	266595
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.566661040154543	27.0	14.0	33.0	14.0	33.0
2	25.743209737616983	27.0	27.0	33.0	14.0	33.0
3	24.879225041729963	27.0	14.0	33.0	14.0	33.0
4	30.45579999624899	33.0	27.0	33.0	27.0	33.0
5	32.08696712241415	33.0	33.0	33.0	33.0	33.0
6	35.655233594028395	37.0	37.0	37.0	33.0	37.0
7	35.647806598023216	37.0	37.0	37.0	33.0	37.0
8	35.995228717717886	37.0	37.0	37.0	33.0	37.0
9	36.17867927005383	37.0	37.0	37.0	37.0	37.0
10-11	36.125548116056194	37.0	37.0	37.0	37.0	37.0
12-13	36.189572197528086	37.0	37.0	37.0	37.0	37.0
14-15	36.179031864813666	37.0	37.0	37.0	37.0	37.0
16-17	36.1847709071813	37.0	37.0	37.0	37.0	37.0
18-19	36.16909919540876	37.0	37.0	37.0	37.0	37.0
20-21	36.19078002213095	37.0	37.0	37.0	37.0	37.0
22-23	36.18455147320843	37.0	37.0	37.0	37.0	37.0
24-25	36.156426414598926	37.0	37.0	37.0	37.0	37.0
26-27	36.11395937658246	37.0	37.0	37.0	37.0	37.0
28-29	36.087634801853	37.0	37.0	37.0	37.0	37.0
30-31	36.03360340591534	37.0	37.0	37.0	37.0	37.0
32-33	35.98086985877455	37.0	37.0	37.0	37.0	37.0
34-35	35.90076708115306	37.0	37.0	37.0	33.0	37.0
36-37	35.82738986102515	37.0	37.0	37.0	33.0	37.0
38-39	35.76028620191677	37.0	37.0	37.0	33.0	37.0
40-41	35.61251711397438	37.0	37.0	37.0	33.0	37.0
42-43	35.57387797970705	37.0	37.0	37.0	33.0	37.0
44-45	35.49185093493877	37.0	37.0	37.0	33.0	37.0
46-47	35.34948142313247	37.0	37.0	37.0	33.0	37.0
48-49	35.20456497683753	37.0	37.0	37.0	33.0	37.0
50-51	35.11926330201241	37.0	37.0	37.0	33.0	37.0
52-53	35.03178791800296	37.0	37.0	37.0	33.0	37.0
54-55	34.97957951199385	37.0	37.0	37.0	30.0	37.0
56-57	34.935522046550005	37.0	37.0	37.0	27.0	37.0
58-59	34.87993585776178	37.0	37.0	37.0	27.0	37.0
60-61	34.86390029820514	37.0	37.0	37.0	27.0	37.0
62-63	34.84880249066937	37.0	37.0	37.0	27.0	37.0
64-65	34.8067311840057	37.0	37.0	37.0	27.0	37.0
66-67	34.77635927155423	37.0	37.0	37.0	27.0	37.0
68-69	34.74043211613121	37.0	37.0	37.0	27.0	37.0
70-71	34.70454247078902	37.0	37.0	37.0	27.0	37.0
72-73	34.69806823083704	37.0	37.0	37.0	27.0	37.0
74-75	34.59301187194058	37.0	37.0	37.0	27.0	37.0
76-77	34.528012528367	37.0	37.0	37.0	27.0	37.0
78-79	34.508831748532415	37.0	37.0	37.0	27.0	37.0
80-81	34.47569346761942	37.0	37.0	37.0	27.0	37.0
82-83	34.42499484236389	37.0	37.0	37.0	27.0	37.0
84-85	34.35120876235489	37.0	37.0	37.0	27.0	37.0
86-87	34.18084547722201	37.0	37.0	37.0	27.0	37.0
88-89	34.10280387854236	37.0	37.0	37.0	27.0	37.0
90-91	34.058465087492266	37.0	37.0	37.0	27.0	37.0
92-93	34.00169733115774	37.0	37.0	37.0	27.0	37.0
94-95	34.00743074701326	37.0	37.0	37.0	27.0	37.0
96-97	33.971460454997285	37.0	37.0	37.0	24.5	37.0
98-99	33.89693542639584	37.0	37.0	37.0	22.0	37.0
100-101	33.84686134398619	37.0	37.0	37.0	22.0	37.0
102-103	33.73249685853073	37.0	33.0	37.0	22.0	37.0
104-105	33.64445319679664	37.0	33.0	37.0	22.0	37.0
106-107	33.56266434104165	37.0	33.0	37.0	22.0	37.0
108-109	33.46619028864008	37.0	33.0	37.0	22.0	37.0
110-111	33.38913332958232	37.0	33.0	37.0	18.0	37.0
112-113	33.34006451733904	37.0	33.0	37.0	14.0	37.0
114-115	33.20748513663047	37.0	33.0	37.0	14.0	37.0
116-117	33.07765336934301	37.0	33.0	37.0	14.0	37.0
118-119	32.93469119825953	37.0	33.0	37.0	14.0	37.0
120-121	32.8431609745119	37.0	33.0	37.0	14.0	37.0
122-123	32.73119713422983	37.0	33.0	37.0	14.0	37.0
124-125	31.428427014760217	37.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	83.0
16	50.0
17	12.0
18	36.0
19	171.0
20	742.0
21	1769.0
22	2905.0
23	3598.0
24	2768.0
25	1932.0
26	2014.0
27	2355.0
28	3001.0
29	3962.0
30	5101.0
31	7193.0
32	10094.0
33	15305.0
34	26094.0
35	57865.0
36	119544.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	55.08456770669188	11.711164137889634	11.689027959119356	21.515240196299132
2	18.41369868152066	19.855211087979896	35.8371312290178	25.893959001481647
3	22.030420675556556	24.768656576454923	28.71171627374857	24.489206474239953
4	15.005907837731392	17.419681539413716	35.34387366604775	32.23053695680714
5	15.712597760648176	35.66721056283876	31.910951068099553	16.709240608413513
6	29.473348587718966	34.61495179864211	18.614726733936006	17.296972879702917
7	25.86586541527404	32.278502380121765	23.06036018800899	18.795272016595206
8	25.928468275849138	31.544477578349184	21.49027551154373	21.036778634257956
9	26.938989853523132	15.652956732121758	21.33348337365667	36.07457004069844
10-11	25.45884206380465	24.902567565033102	26.525441212325813	23.11314915883644
12-13	26.746938239651907	24.026332076745625	26.672668279600142	22.554061404002326
14-15	25.02091187006508	24.088223710122094	25.446651287533523	25.444213132279298
16-17	23.458992104127987	26.21973405352688	26.501059659783564	23.820214182561564
18-19	23.293572647649054	24.917946698175133	29.056058815806747	22.732421838369063
20-21	24.659736041066115	23.948918676116723	28.9636507879945	22.42769449482266
22-23	24.55241096044562	23.83109210600349	28.687897372418835	22.928599561132053
24-25	23.716498809054933	24.729833642791498	28.67008008402258	22.883587464130986
26-27	23.304825671899323	24.74446257431685	28.6342579568259	23.31645379695793
28-29	23.011121738967347	24.583356777133854	29.419343948686212	22.986177535212587
30-31	23.455803747257075	24.367486261932893	29.26536506686172	22.911344923948313
32-33	23.06851216264371	24.4856430165607	28.903767887619797	23.54207693317579
34-35	23.293385097244883	24.566852341566797	28.952343442300116	23.1874191188882
36-37	23.474558787674187	24.575667210562838	28.538794801102796	23.41097920066018
38-39	23.510568465275043	24.468200828972787	28.700838350306647	23.320392355445527
40-41	22.943010079109946	24.615234572810035	28.910578376614364	23.531176971465655
42-43	23.443988071794294	24.506836212232034	28.484592734297344	23.564582981676324
44-45	23.51807048144189	24.591233894109042	28.165944597610608	23.72475102683846
46-47	23.489086990852844	24.636373696243794	27.94713140812235	23.927407904781017
48-49	23.820277405257386	24.79260252646979	27.845560859412366	23.541559208860463
50-51	23.335208837375042	25.2240289577824	27.58041223578837	23.860349969054184
52-53	23.336334139800073	25.088617565970857	27.254824734147302	24.32022356008177
54-55	23.400851478834937	25.15182205217652	27.157486074382494	24.28984039460605
56-57	23.728502034921885	24.79885219152647	26.873909863275756	24.598735910275888
58-59	23.279881468144563	24.955456779009356	27.36285376694987	24.40180798589621
60-61	23.440424614115045	24.899754308970536	27.621673324706013	24.038147752208406
62-63	23.087642303869167	24.946641910013316	27.627487387235323	24.3382283988822
64-65	22.91003207111911	24.958082484667756	27.713760573154033	24.418124871059096
66-67	23.46540874604946	24.910579475011957	27.5976029484859	24.026408830452684
68-69	23.30295016785761	24.64468575929781	27.678125996361523	24.374238076483053
70-71	22.905530861419006	24.772970235750858	27.935820251692643	24.385678651137493
72-73	23.38115868639697	24.72458223147471	28.013841219827828	23.880417862300494
74-75	23.29082087521233	24.627945606576763	28.165770432726145	23.915463085484763
76-77	23.268187710947803	24.688233451092003	28.199418023267814	23.84416081469238
78-79	23.065785426528727	24.898534850746827	28.01375874925917	24.021920973465267
80-81	22.73767324968585	24.93932744425064	28.203829779253176	24.11916952681033
82-83	23.05275792869334	24.747088279975245	28.309420656801514	23.890733134529903
84-85	23.037358538008736	25.040672374872635	27.801327411210373	24.120641675908256
86-87	22.80672411038967	25.009443447375094	27.908440844554278	24.275391597680954
88-89	23.262466801871312	24.42724417470345	27.92947054036122	24.380818483064022
90-91	23.350573158456154	24.582525803398386	27.723253857363922	24.343647180781538
92-93	23.086412335284816	24.740980799345085	27.751011104309914	24.421595761060185
94-95	22.311975678417973	24.93843646436817	28.1200474878514	24.62954036936246
96-97	22.982426527129167	24.732834449258238	27.562032296179602	24.722706727432996
98-99	22.644085598004462	25.07455128565802	27.303962940040137	24.97740017629738
100-101	22.994054652187774	25.13494251580112	27.00950880549148	24.861494026519626
102-103	23.033815337872056	25.08074044899567	27.302087435998423	24.583356777133854
104-105	23.036180823469614	25.029492688251505	27.074944249910445	24.85938223836844
106-107	22.828773880392664	25.018099021339967	27.28075802077355	24.87236907749382
108-109	23.019415727639654	25.259201464432007	26.817598691614027	24.903784116314313
110-111	22.755806124305355	25.47061349957895	26.87068514423645	24.902895231879246
112-113	22.581818863819652	25.604943828653955	26.547759710422174	25.265477597104223
114-115	22.833511506217295	25.408391005082613	26.688610063954687	25.0694874247454
116-117	22.89465293797708	25.46296817269641	26.532005476471802	25.110373412854702
118-119	22.4801665447589	25.319304563101337	26.933925992610515	25.26660289952925
120-121	22.536370916770288	25.312882722196907	27.13044391554933	25.020302445483477
122-123	22.51617622235976	25.533862225473097	27.00688309983308	24.943078452334063
124-125	22.487105909713236	25.404452446595023	26.949867776965057	25.15857386672668
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	58.0
1	50.0
2	102.5
3	165.0
4	136.5
5	136.5
6	183.5
7	296.5
8	493.5
9	611.5
10	562.0
11	491.0
12	472.5
13	450.0
14	468.5
15	496.5
16	488.0
17	484.5
18	477.0
19	464.0
20	472.0
21	465.0
22	474.5
23	514.0
24	518.5
25	551.0
26	636.5
27	717.5
28	824.0
29	1033.0
30	1397.0
31	1773.0
32	2221.5
33	2770.0
34	3266.0
35	3803.0
36	4410.5
37	5116.5
38	5654.5
39	5866.5
40	5893.0
41	5985.5
42	6209.5
43	6855.5
44	7812.0
45	8673.0
46	9606.0
47	10401.5
48	11257.5
49	12170.5
50	12761.5
51	12389.5
52	11593.0
53	11296.5
54	10869.0
55	10160.0
56	9606.5
57	8670.5
58	7787.0
59	7134.5
60	6176.0
61	5059.0
62	3929.5
63	3100.5
64	2436.5
65	1899.5
66	1427.0
67	1022.5
68	794.5
69	665.5
70	580.0
71	505.5
72	433.0
73	438.5
74	371.5
75	297.0
76	245.0
77	155.5
78	112.0
79	90.5
80	60.5
81	36.5
82	32.5
83	25.0
84	12.0
85	7.0
86	2.5
87	2.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023631350925561245
2	0.0
3	0.0
4	0.0
5	0.0
6	0.00187550404171121
7	0.004501209700106904
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.87550404171121E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.001500403233368968
42-43	0.0
44-45	0.0
46-47	0.0035634576792512988
48-49	0.006564264145989235
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.006564264145989235
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.075957913689304
76-77	0.03357152234663066
78-79	3.75100808342242E-4
80-81	0.0
82-83	0.0
84-85	0.051201260338716026
86-87	0.20161668448395506
88-89	0.216995817625987
90-91	0.13166038372812694
92-93	0.11328044411935706
94-95	1.87550404171121E-4
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0013128528291978469
106-107	0.002250604850053452
108-109	0.0026257056583956937
110-111	5.62651212513363E-4
112-113	0.0
114-115	0.0
116-117	0.0
118-119	0.0
120-121	5.62651212513363E-4
122-123	0.0
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	266595.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.14759300077446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.8001737522199	61.75623434519449
2	8.803514890388461	12.526977887952317
3	1.8628796612603404	3.976182118463139
4	0.7310906762672157	2.0806136752688333
5	0.40376409668884167	1.4363421809771526
6	0.23409432545444925	0.9993148674734396
7	0.16456364966785916	0.8195815298503615
8	0.11938577242280032	0.6795208277136385
9	0.10000969884794565	0.6403904414787295
>10	0.7195772140425672	9.957309398230306
>50	0.04570969705474234	2.150825528202104
>100	0.012979296694556467	1.5794936770481753
>500	0.0011286344951788232	0.41916405664420314
>1k	0.0011286344951788232	0.9780494655031406
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1251	0.4692511112361447	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1185	0.4444944578855568	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	532	0.19955363003807272	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	512	0.1920516138712279	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	424	0.15904274273711058	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	350	0.1312852829197847	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	306	0.11478084735272605	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.75100808342242E-4	0.0
3	0.0	0.0	0.0	3.75100808342242E-4	0.0
4	0.0	0.0	0.0	3.75100808342242E-4	0.0
5	0.0	0.0	0.0	3.75100808342242E-4	0.0
6	0.0	0.0	0.0	3.75100808342242E-4	0.0
7	0.0	0.0	0.0	3.75100808342242E-4	0.0
8	0.0	0.0	0.0	3.75100808342242E-4	0.0
9	0.0	0.0	0.0	3.75100808342242E-4	0.0
10-11	0.0	0.0	0.0	3.75100808342242E-4	0.0
12-13	0.0	0.0	0.0	7.50201616684484E-4	0.0
14-15	0.0	0.0	0.0	7.50201616684484E-4	0.0
16-17	0.0	0.0	0.0	7.50201616684484E-4	0.0
18-19	0.0	0.0	0.0	7.50201616684484E-4	0.0
20-21	0.0	0.0	0.0	0.001125302425026726	0.0
22-23	0.0	0.0	0.0	0.0052514113167913875	0.0
24-25	0.0	0.0	0.0	0.011253024250267259	0.0
26-27	0.0	0.0	0.0	0.01856749001294098	0.0
28-29	0.0	0.0	0.0	0.03507192557999962	0.0
30-31	3.75100808342242E-4	0.0	0.0	0.05907837731390311	0.0
32-33	3.75100808342242E-4	0.0	0.0	0.09902661340235189	0.0
34-35	3.75100808342242E-4	0.0	0.0	0.14103790393668297	0.0
36-37	3.75100808342242E-4	0.0	0.0	0.2091187006507999	0.0
38-39	3.75100808342242E-4	0.0	0.0	0.30064329788630695	0.0
40-41	3.75100808342242E-4	0.0	0.0	0.40698437705133256	0.0
42-43	3.75100808342242E-4	0.0	0.0	0.5078864944953957	0.0
44-45	3.75100808342242E-4	0.0	0.0	0.5956600836474802	1.87550404171121E-4
46-47	3.75100808342242E-4	0.0	0.0	0.699187906749939	3.75100808342242E-4
48-49	3.75100808342242E-4	0.0	0.0	0.807779590765018	3.75100808342242E-4
50-51	3.75100808342242E-4	0.0	0.0	0.903805397700632	7.50201616684484E-4
52-53	3.75100808342242E-4	0.0	0.0	0.9970179485736792	7.50201616684484E-4
54-55	3.75100808342242E-4	0.0	0.0	1.0930437555092931	7.50201616684484E-4
56-57	3.75100808342242E-4	0.0	0.0	1.2066993004369926	7.50201616684484E-4
58-59	3.75100808342242E-4	0.0	0.0	1.3271066599148522	7.50201616684484E-4
60-61	3.75100808342242E-4	0.0	0.0	1.4559537875804123	7.50201616684484E-4
62-63	3.75100808342242E-4	0.0	0.0	1.5643579211913203	0.001125302425026726
64-65	3.75100808342242E-4	0.0	0.0	1.681952024606613	0.001125302425026726
66-67	3.75100808342242E-4	0.0	0.0	1.8074232449970928	0.001125302425026726
68-69	3.75100808342242E-4	0.0	0.0	1.9388960783210487	0.001125302425026726
70-71	3.75100808342242E-4	0.0	0.0	2.0949380145914214	0.001125302425026726
72-73	3.75100808342242E-4	0.0	0.0	2.2427277330782647	0.001125302425026726
74-75	3.75100808342242E-4	0.0	0.0	2.3661358990228623	0.001125302425026726
76-77	3.75100808342242E-4	0.0	0.0	2.5154260207430745	0.001125302425026726
78-79	3.75100808342242E-4	0.0	0.0	2.679907725201148	0.001125302425026726
80-81	3.75100808342242E-4	0.0	0.0	2.8503910425926966	0.001125302425026726
82-83	3.75100808342242E-4	0.0	0.0	3.0219996624092724	0.001125302425026726
84-85	3.75100808342242E-4	0.0	0.0	3.190232374950768	0.001125302425026726
86-87	3.75100808342242E-4	0.0	0.0	3.3594028395131192	0.001125302425026726
88-89	3.75100808342242E-4	0.0	0.0	3.5407640803465927	0.001125302425026726
90-91	5.62651212513363E-4	0.0	0.0	3.735441399876217	0.001125302425026726
92-93	7.50201616684484E-4	0.0	0.0	3.9205536487931134	0.001125302425026726
94-95	7.50201616684484E-4	0.0	0.0	4.104915696093325	0.001125302425026726
96-97	7.50201616684484E-4	0.0	0.0	4.295466906731184	0.001125302425026726
98-99	7.50201616684484E-4	0.0	0.0	4.508149065061236	0.001125302425026726
100-101	7.50201616684484E-4	0.0	0.0	4.737148108554173	0.001125302425026726
102-103	7.50201616684484E-4	0.0	0.0	4.968772857705508	0.001125302425026726
104-105	7.50201616684484E-4	0.0	0.0	5.192520489881655	0.001125302425026726
106-107	7.50201616684484E-4	0.0	0.0	5.420581781353739	0.001125302425026726
108-109	7.50201616684484E-4	0.0	0.0	5.663834655563683	0.001125302425026726
110-111	7.50201616684484E-4	0.0	0.0	5.907275080177798	0.001125302425026726
112-113	7.50201616684484E-4	0.0	0.0	6.169658095613196	0.001125302425026726
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCAGA	155	0.0	57.560314	1
GCGTACA	35	0.0078006065	50.98199	1
GTCAACG	75	1.4701072E-6	47.58319	1
CTATTGA	180	2.1926098E-8	29.739496	9
GGGCTAT	160	2.5983172E-7	29.739494	6
CACTTAA	105	5.9058424E-4	28.323328	7
GTATCAA	2465	0.0	27.990114	1
GGTATCA	1890	0.0	27.693922	1
GCTTTAG	130	6.3755084E-5	27.451841	1
ACTTTAT	110	7.751512E-4	27.035906	8
GGATAAG	115	0.0010048525	25.860432	1
GATAACC	165	1.1087008E-5	25.23351	2
ATCAACG	2775	0.0	24.863289	3
GGTTTCA	120	0.0012879171	24.782913	1
CAACGCA	2870	0.0	24.662022	5
GGCTATT	195	1.4622565E-6	24.401638	7
TATCAAC	2885	0.0	24.121462	2
TCAACGC	2960	0.0	23.91216	4
AACGCAG	2970	0.0	23.831648	6
TTTAGGG	200	1.8226856E-6	23.791597	2
>>END_MODULE