FastQCFastQC Report
Sun 1 Jan 2017
ERR1142451_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142451_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310103
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6850.22089434800695254No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4340.13995349932119328No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3890.12544219178788982No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3490.11254325175828676No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA1051.09139364E-1051.107611
CTAACGC800.0053181229.7934553
ATGGGGG8900.024.1025715
CATGGGG13050.023.2868394
GGTATCA19600.022.5116861
TATTCTC1350.00252955922.0692255
TGGGGGG4950.021.660956
AACGCCT1500.00465873919.8623035
TAGTGTA1550.005615449519.230939
TACATAA1550.00563125119.2215842
GTATCAA22900.018.7347492
GTACTTG1600.00673912718.6329821
AAAGCAG3553.9119186E-818.4635495
ACATGGG29200.017.9576993
GTACATG31400.017.4699061
CATGGGC4451.551598E-917.4074134
TACATGG31600.017.1595212
ATACCCA2100.001612460717.0193186
TAACCTT1050.001620191417.01105324-25
TCCCTTA1050.00162722717.00004834-35
Produced by FastQC (version 0.11.5)