##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142451_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	310103
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.88654414823462	33.0	33.0	33.0	27.0	33.0
2	30.96258339970913	33.0	33.0	33.0	27.0	33.0
3	31.09217905018655	33.0	33.0	33.0	27.0	33.0
4	31.03790353527699	33.0	33.0	33.0	27.0	33.0
5	31.095252222648604	33.0	33.0	33.0	27.0	33.0
6	34.321270674582316	37.0	37.0	37.0	27.0	37.0
7	34.30873612960855	37.0	37.0	37.0	27.0	37.0
8	34.33105774532978	37.0	37.0	37.0	27.0	37.0
9	34.23922696652403	37.0	37.0	37.0	27.0	37.0
10-11	34.35096403453046	37.0	37.0	37.0	27.0	37.0
12-13	34.285593818827934	37.0	37.0	37.0	27.0	37.0
14-15	34.246289136190235	37.0	37.0	37.0	27.0	37.0
16-17	34.22736639116681	37.0	37.0	37.0	27.0	37.0
18-19	34.277085355510906	37.0	37.0	37.0	27.0	37.0
20-21	34.2429192881075	37.0	37.0	37.0	27.0	37.0
22-23	34.35604944163713	37.0	37.0	37.0	27.0	37.0
24-25	34.454503826148084	37.0	37.0	37.0	27.0	37.0
26-27	34.495182245898945	37.0	37.0	37.0	27.0	37.0
28-29	34.544514242042155	37.0	37.0	37.0	27.0	37.0
30-31	34.51279413614186	37.0	37.0	37.0	27.0	37.0
32-33	34.531657223567656	37.0	37.0	37.0	27.0	37.0
34-35	34.59385107528789	37.0	37.0	37.0	27.0	37.0
36-37	34.57567001931616	37.0	37.0	37.0	27.0	37.0
38-39	34.54580413604512	37.0	37.0	37.0	27.0	37.0
40-41	34.5020944653873	37.0	37.0	37.0	27.0	37.0
42-43	34.5026136477235	37.0	37.0	37.0	27.0	37.0
44-45	34.47722853374524	37.0	37.0	37.0	27.0	37.0
46-47	34.45913454561871	37.0	37.0	37.0	27.0	37.0
48-49	34.43861878150163	37.0	37.0	37.0	27.0	37.0
50-51	34.36479653534471	37.0	37.0	37.0	27.0	37.0
52-53	34.37143787709245	37.0	37.0	37.0	27.0	37.0
54-55	34.35122362569856	37.0	37.0	37.0	27.0	37.0
56-57	34.31880536466916	37.0	37.0	37.0	27.0	37.0
58-59	34.260203867747165	37.0	37.0	37.0	27.0	37.0
60-61	34.257383836983195	37.0	37.0	37.0	27.0	37.0
62-63	34.21903690064269	37.0	37.0	37.0	27.0	37.0
64-65	34.16537731011954	37.0	37.0	37.0	27.0	37.0
66-67	34.13785580919888	37.0	37.0	37.0	27.0	37.0
68-69	34.09216453887902	37.0	37.0	37.0	27.0	37.0
70-71	34.031860381873116	37.0	37.0	37.0	27.0	37.0
72-73	33.962119037868064	37.0	37.0	37.0	27.0	37.0
74-75	33.87502217005317	37.0	37.0	37.0	27.0	37.0
76-77	33.83076429444411	37.0	37.0	37.0	27.0	37.0
78-79	33.777001512400716	37.0	37.0	37.0	22.0	37.0
80-81	33.7169053508028	37.0	37.0	37.0	22.0	37.0
82-83	33.63386519962722	37.0	33.0	37.0	22.0	37.0
84-85	33.569101234106085	37.0	33.0	37.0	22.0	37.0
86-87	33.47990828853639	37.0	33.0	37.0	18.0	37.0
88-89	33.41988307110863	37.0	33.0	37.0	14.0	37.0
90-91	33.30740431405049	37.0	33.0	37.0	14.0	37.0
92-93	33.21711495857828	37.0	33.0	37.0	14.0	37.0
94-95	33.151440005417555	37.0	33.0	37.0	14.0	37.0
96-97	33.04668932580465	37.0	33.0	37.0	14.0	37.0
98-99	32.94907176002812	37.0	33.0	37.0	14.0	37.0
100-101	32.83112224002993	37.0	33.0	37.0	14.0	37.0
102-103	32.713570974805144	37.0	33.0	37.0	14.0	37.0
104-105	32.5730692705327	37.0	33.0	37.0	14.0	37.0
106-107	32.48040973483004	37.0	33.0	37.0	14.0	37.0
108-109	32.354833716539346	37.0	33.0	37.0	14.0	37.0
110-111	32.25432356346117	37.0	33.0	37.0	14.0	37.0
112-113	32.11921039138609	37.0	33.0	37.0	14.0	37.0
114-115	32.00547721241007	37.0	33.0	37.0	14.0	37.0
116-117	31.781627394768833	37.0	27.0	37.0	14.0	37.0
118-119	31.679851533200257	37.0	27.0	37.0	14.0	37.0
120-121	31.579512291077478	37.0	27.0	37.0	14.0	37.0
122-123	31.494695310912824	37.0	27.0	37.0	14.0	37.0
124-125	29.911248843126316	35.0	24.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	606.0
1	38.0
2	23.0
3	18.0
4	14.0
5	10.0
6	12.0
7	15.0
8	13.0
9	17.0
10	17.0
11	5.0
12	14.0
13	6.0
14	35.0
15	143.0
16	214.0
17	416.0
18	1064.0
19	1850.0
20	2893.0
21	3479.0
22	3629.0
23	3703.0
24	3917.0
25	4317.0
26	4968.0
27	5880.0
28	6678.0
29	7993.0
30	9465.0
31	11446.0
32	14370.0
33	18598.0
34	27073.0
35	48521.0
36	128643.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.64047868826981	20.007835671083136	12.005413736384712	24.346271904262345
2	17.34191226446238	23.046275738411644	35.50913669111317	24.102675306012802
3	19.825050242230656	27.698760206211592	27.769956925984534	24.706232625573215
4	14.562117137670255	19.492202795279283	33.95497393381032	31.99070613324014
5	15.246464057999159	37.80496488332201	31.05285302780205	15.895718030876784
6	29.438992359893646	36.24069246145984	17.986919309867446	16.33339586877907
7	25.93191558179025	33.68901107786852	21.473922535780705	18.905150804560524
8	25.4863278407529	34.052812858783014	20.278132630455524	20.18272667000857
9	26.108782280184233	18.56595766702893	19.723257258189722	35.60200279459711
10-11	25.110415706442197	27.584115663585862	25.096992779111982	22.20847585085996
12-13	25.96766600173755	26.436352046800653	24.784059820970665	22.811922130491126
14-15	24.463616571840173	26.509764565915876	23.03822465380911	25.988394208434833
16-17	23.889138785244825	28.209774866754906	23.92538274593307	23.9757036020672
18-19	24.012055287372686	27.325479817261773	25.634795325421578	23.027669569943964
20-21	25.724420835368754	26.242199353626066	24.771682480937145	23.261697330068035
22-23	25.8509475100768	25.870842310255366	24.375820862690293	23.902389316977544
24-25	24.755280491492833	26.680692993251515	24.726494717487036	23.83753179776862
26-27	24.434755044346257	26.68651627970164	24.704681748491065	24.174046927461042
28-29	24.12703495094448	26.536390995195518	25.34877365248932	23.987800401370677
30-31	24.50413911434758	26.45168176283093	25.21164547381903	23.832533649002457
32-33	24.193681405310826	26.743499725904236	24.77243583005739	24.290383038727548
34-35	24.212531266724767	26.59341050238573	25.120418323311274	24.07363990757823
36-37	24.32236647430062	26.80991981424199	24.701708656795578	24.166005054661817
38-39	24.224722225815214	26.69299970250029	25.004365484859854	24.07791258682464
40-41	23.88942789689855	26.54943242456583	25.343294201351835	24.21784547718379
42-43	24.10303424234487	26.70540717960658	25.03581395499245	24.1557446230561
44-45	24.206639202726564	26.68938801114941	24.95004106656664	24.153931719557388
46-47	24.110562659557097	26.776651592781995	24.96681535757766	24.14597039008325
48-49	24.38957356238774	26.8008853062762	25.023159262977675	23.786381868358383
50-51	23.712988621335242	27.104761966352264	24.94608149051131	24.236167921801187
52-53	23.74787796478634	26.805548818935833	24.834036636432717	24.612536579845113
54-55	23.83160495264334	26.75352224259416	24.734113934079012	24.680758870683494
56-57	24.092840374827816	26.436969301110384	24.660320375603856	24.80986994845794
58-59	23.902327829681568	26.47782992406545	25.211822962724657	24.40801928352833
60-61	24.07435547594876	26.47220479925509	25.161656396096966	24.291783328699186
62-63	23.520691645260218	26.673704256340702	24.993089043830818	24.812515054568262
64-65	23.451750883443637	26.32597372785733	25.17288983870081	25.04938554999822
66-67	24.039708121704326	26.115639154626308	24.875285719366197	24.969367004303166
68-69	23.904830112665447	26.002777431990907	24.860117984276307	25.23227447106734
70-71	23.685639923050807	26.08335084627944	25.02869428862413	25.202314942045618
72-73	24.076792024026197	25.987864054130068	25.320451687362	24.614892234481733
74-75	24.21219744049043	25.854732635363654	25.291000329800372	24.64206959434554
76-77	23.76000517439322	25.849813236744662	25.351777889170968	25.038403699691152
78-79	23.74762750062243	26.0392921464208	25.29528927517113	24.917791077785644
80-81	23.49378500381566	26.118020255325753	25.446723060804782	24.941471680053805
82-83	23.93309416837364	25.895938991918076	25.541556663179964	24.629410176528314
84-85	23.934127796343272	26.091012414899172	25.14247466982253	24.832385118935026
86-87	23.64348228112886	26.17966848042211	25.274407296405144	24.902441942043886
88-89	24.07410101362764	25.493599970258735	25.397587401340623	25.034711614773002
90-91	24.10222345055865	25.60273337179966	25.44563560753358	24.84940757010811
92-93	23.937590514120203	25.86473311265129	25.498797455260164	24.698878917968344
94-95	23.026088249459388	25.99063266671623	25.88784412343676	25.095434960387625
96-97	23.430954905104205	26.016024978021406	25.441026270879664	25.111993845994725
98-99	23.429338832290426	26.391438692661733	25.22495733567772	24.95426513937012
100-101	23.662931806209542	26.574604026151565	24.956774942070343	24.80568922556855
102-103	23.56759534416979	26.715435567039464	25.337043518729484	24.37992557006126
104-105	23.48662489758387	26.888537311792682	25.185561062639078	24.439276727984367
106-107	23.384612898534332	26.90561220367468	25.324240906226265	24.385533991564724
108-109	23.441769670683406	27.039705765908728	24.991637404314936	24.52688715909293
110-111	23.109192774024237	27.605631982004546	24.861874881832126	24.423300362139095
112-113	23.193120064378203	27.5006746460734	24.56438870602075	24.741816583527648
114-115	23.191131491059267	27.457025184639424	24.8601975411571	24.49164578314421
116-117	23.391036113117487	27.34588219325503	24.73463346373418	24.528448229893304
118-119	23.227880824662314	27.193336780197765	24.93310928714535	24.64567310799457
120-121	23.130167275523938	27.239105601863894	25.20200543205169	24.428721690560476
122-123	23.233436192384122	27.54043006930869	25.144352714994266	24.081781023312924
124-125	23.099168613461806	27.401343530985546	24.885050228930183	24.614437626622465
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	816.0
1	462.0
2	86.5
3	62.0
4	62.0
5	57.0
6	41.0
7	34.5
8	30.0
9	35.5
10	42.0
11	37.5
12	40.5
13	47.0
14	57.0
15	76.0
16	89.5
17	91.0
18	99.5
19	119.0
20	141.0
21	169.5
22	213.0
23	283.5
24	365.0
25	447.0
26	584.0
27	795.5
28	993.5
29	1346.0
30	1911.0
31	2469.5
32	3027.5
33	3605.5
34	4266.5
35	4854.0
36	5366.0
37	6161.0
38	6643.0
39	6851.0
40	6925.5
41	6904.0
42	7276.5
43	8031.5
44	9085.5
45	10231.5
46	11517.5
47	12692.0
48	13780.5
49	14267.5
50	14614.0
51	14599.0
52	13825.0
53	13720.0
54	13387.5
55	12452.5
56	11723.5
57	10413.0
58	9093.0
59	8150.5
60	6935.0
61	5760.0
62	4731.5
63	3712.0
64	2769.5
65	2065.0
66	1577.0
67	1293.5
68	1068.5
69	869.0
70	756.0
71	671.0
72	590.0
73	511.5
74	390.0
75	328.5
76	264.0
77	178.0
78	138.5
79	109.5
80	73.5
81	46.0
82	34.5
83	23.5
84	16.0
85	10.0
86	6.0
87	2.0
88	1.0
89	1.5
90	1.5
91	1.0
92	1.0
93	1.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.40599413743175655
2	0.36407258233554657
3	0.3547208508140844
4	0.348916327800763
5	0.3653624763385069
6	0.3047374581993725
7	0.2992554086867911
8	0.2895812036645889
9	0.3015127231919717
10-11	0.3000615924386414
12-13	0.33779099202523033
14-15	0.38874180514216244
16-17	0.35020622180372324
18-19	0.33118028526005877
20-21	0.3205386597356362
22-23	0.3150566102230549
24-25	0.29748180443272076
26-27	0.3047374581993725
28-29	0.29425706942532
30-31	0.29619191042976045
32-33	0.29167728141939936
34-35	0.2800682353927566
36-37	0.28490533790385775
38-39	0.2770047371357259
40-41	0.2879688361608885
42-43	0.2792620516409064
44-45	0.2737800021283251
46-47	0.27361876537795504
48-49	0.2681367158653738
50-51	0.2708777406216644
52-53	0.27345752862758504
54-55	0.2753923696320255
56-57	0.27120021412240447
58-59	0.2655569278594532
60-61	0.2595911680957617
62-63	0.26168724585057224
64-65	0.2586237475935415
66-67	0.25668890658910104
68-69	0.2658794013601932
70-71	0.260558588597982
72-73	0.24959448957281935
74-75	0.2660406381105632
76-77	0.28635646865718806
78-79	0.26765300561426364
80-81	0.27442494912980525
82-83	0.2684591893661138
84-85	0.2700715568698142
86-87	0.25797880059206135
88-89	0.24782088531874893
90-91	0.23911410079876685
92-93	0.24475738706171818
94-95	0.23540565554025597
96-97	0.22927865902619454
98-99	0.22927865902619454
100-101	0.21799208650029184
102-103	0.22073311125658251
104-105	0.22686010777064397
106-107	0.22186176850917277
108-109	0.22234547876028285
110-111	0.22299042576176303
112-113	0.21976569075436228
114-115	0.20912406522993976
116-117	0.21686342924770158
118-119	0.20735046097586932
120-121	0.20751169772623934
122-123	0.1992886234573674
124-125	0.19993357045884752
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	310103.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.90427997199626
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.71733801162381	62.195346927744055
2	8.148379348578334	11.555099412992616
3	1.796255709638744	3.82086653212567
4	0.6270689435048752	1.778474877280543
5	0.31662008378843387	1.1224859532846017
6	0.20675350382016303	0.8795824992033624
7	0.15247183620979093	0.7567634033724363
8	0.10899777934828278	0.6182727250589213
9	0.08570081719587729	0.546889926265682
>10	0.7505975583937771	10.776191957571628
>50	0.06699013848739524	3.18101727414661
>100	0.022330046162465	2.5279956990093275
>500	4.962232480547777E-4	0.24101281194452182
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	685	0.22089434800695254	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	434	0.13995349932119328	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	389	0.12544219178788982	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	349	0.11254325175828676	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	6.449470014801533E-4	0.0
14-15	0.0	0.0	0.0	6.449470014801533E-4	0.0
16-17	0.0	0.0	0.0	6.449470014801533E-4	0.0
18-19	0.0	0.0	0.0	6.449470014801533E-4	0.0
20-21	0.0	0.0	0.0	0.0017736042540704216	3.2247350074007667E-4
22-23	0.0	0.0	0.0	0.003224735007400767	3.2247350074007667E-4
24-25	0.0	0.0	0.0	0.008384311019241994	3.2247350074007667E-4
26-27	0.0	0.0	0.0	0.013221413530343144	3.2247350074007667E-4
28-29	0.0	0.0	0.0	0.029345088567346977	3.2247350074007667E-4
30-31	0.0	0.0	0.0	0.054659258375443	3.2247350074007667E-4
32-33	0.0	0.0	0.0	0.0933560784642522	4.83710251110115E-4
34-35	0.0	0.0	0.0	0.13431021305824192	6.449470014801533E-4
36-37	0.0	0.0	0.0	0.2044481994692086	6.449470014801533E-4
38-39	0.0	0.0	0.0	0.2950632531771702	6.449470014801533E-4
40-41	0.0	0.0	0.0	0.3843884128821714	6.449470014801533E-4
42-43	0.0	0.0	0.0	0.4654904983183007	6.449470014801533E-4
44-45	0.0	0.0	0.0	0.54707629400554	9.6742050222023E-4
46-47	0.0	0.0	0.0	0.6481717364875541	0.001451130753330345
48-49	0.0	0.0	0.0	0.7431401824555067	0.0016123675037003835
50-51	0.0	0.0	0.0	0.832626578910878	0.0016123675037003835
52-53	0.0	0.0	0.0	0.9237253428699497	0.0016123675037003835
54-55	0.0	0.0	0.0	1.0206286298423428	0.0016123675037003835
56-57	0.0	0.0	0.0	1.1238201500791671	0.0016123675037003835
58-59	0.0	0.0	0.0	1.2416842145996654	0.0016123675037003835
60-61	0.0	0.0	0.0	1.3522926253535115	0.0016123675037003835
62-63	0.0	0.0	0.0	1.4527431208340453	0.0016123675037003835
64-65	0.0	0.0	0.0	1.5488402240545882	0.0016123675037003835
66-67	0.0	0.0	0.0	1.6552564792988136	0.0016123675037003835
68-69	0.0	0.0	0.0	1.7789250668326329	0.0016123675037003835
70-71	0.0	0.0	0.0	1.9162987781479057	0.0016123675037003835
72-73	0.0	0.0	0.0	2.045449415194306	0.0016123675037003835
74-75	0.0	0.0	0.0	2.166215741221465	0.0016123675037003835
76-77	0.0	0.0	0.0	2.303105742285628	0.0016123675037003835
78-79	0.0	0.0	0.0	2.4538621038816135	0.0016123675037003835
80-81	0.0	0.0	0.0	2.6047797022279697	0.0017736042540704216
82-83	0.0	0.0	0.0	2.766338926098748	0.00193484100444046
84-85	0.0	0.0	0.0	2.910484580929562	0.00193484100444046
86-87	0.0	0.0	0.0	3.053662815258156	0.00193484100444046
88-89	0.0	0.0	0.0	3.2174793536341153	0.00193484100444046
90-91	0.0	0.0	0.0	3.393227411537457	0.00193484100444046
92-93	0.0	0.0	0.0	3.5536579781556448	0.00193484100444046
94-95	0.0	0.0	0.0	3.720376778038265	0.0022573145051805366
96-97	0.0	0.0	0.0	3.9043479102104786	0.0022573145051805366
98-99	0.0	0.0	0.0	4.108312399428577	0.0022573145051805366
100-101	0.0	0.0	0.0	4.3284005636836795	0.0022573145051805366
102-103	0.0	0.0	0.0	4.535912261409918	0.0022573145051805366
104-105	0.0	0.0	0.0	4.734233464365066	0.0022573145051805366
106-107	0.0	0.0	0.0	4.937230533080944	0.0022573145051805366
108-109	0.0	0.0	0.0	5.154093962328646	0.0022573145051805366
110-111	0.0	0.0	0.0	5.400624953644434	0.0022573145051805366
112-113	0.0	0.0	0.0	5.670212800263139	0.0022573145051805366
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATA	105	1.09139364E-10	51.10761	1
CTAACGC	80	0.00531812	29.793455	3
ATGGGGG	890	0.0	24.102571	5
CATGGGG	1305	0.0	23.286839	4
GGTATCA	1960	0.0	22.511686	1
TATTCTC	135	0.002529559	22.069225	5
TGGGGGG	495	0.0	21.66095	6
AACGCCT	150	0.004658739	19.862303	5
TAGTGTA	155	0.0056154495	19.23093	9
TACATAA	155	0.005631251	19.221584	2
GTATCAA	2290	0.0	18.734749	2
GTACTTG	160	0.006739127	18.632982	1
AAAGCAG	355	3.9119186E-8	18.463549	5
ACATGGG	2920	0.0	17.957699	3
GTACATG	3140	0.0	17.469906	1
CATGGGC	445	1.551598E-9	17.407413	4
TACATGG	3160	0.0	17.159521	2
ATACCCA	210	0.0016124607	17.019318	6
TAACCTT	105	0.0016201914	17.011053	24-25
TCCCTTA	105	0.001627227	17.000048	34-35
>>END_MODULE