FastQCFastQC Report
Sun 1 Jan 2017
ERR1142451_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142451_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310103
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15820.5101530781708014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15270.4924170356300971No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7010.22605392401879373No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5350.17252332289594102No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4860.15672212135967725No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4050.13060176779973107No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3330.10738367574644554No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG709.146115E-750.9783671
TATCACG350.007808970850.9701462
TACCCCG350.007808970850.9701468
GCGTAAG602.1951484E-549.56231
GCGCAGA1550.046.0449751
ACTTATG650.001942294636.5939528
GCCCAAC750.003914241731.7198731
GTATCAA23300.031.1412931
GGTATCA16850.029.6491411
ATGGGGC2853.6379788E-1227.1244645
CAACGCA28000.026.9717035
GGCCGAA450.009545363526.43322840-41
ATCAACG28100.026.240863
TATCAAC27900.026.0026912
AACGCAG29600.025.9155656
TCAACGC30000.025.3718094
CATGGGG11250.025.3718074
GTGTAAA1451.3408504E-424.6102471
GTCTTAT1701.3990737E-524.4896071
GTGTTAT1250.001634878923.7899061
Produced by FastQC (version 0.11.5)