##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142451_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	310103
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.511920232954857	27.0	14.0	33.0	14.0	33.0
2	25.70245692560214	27.0	27.0	33.0	14.0	33.0
3	24.87104607178905	27.0	14.0	33.0	14.0	33.0
4	30.380947620629275	33.0	27.0	33.0	27.0	33.0
5	32.05126683714766	33.0	33.0	33.0	33.0	33.0
6	35.63386036252471	37.0	37.0	37.0	33.0	37.0
7	35.632306040251144	37.0	37.0	37.0	33.0	37.0
8	35.98965182536125	37.0	37.0	37.0	33.0	37.0
9	36.151765703653304	37.0	37.0	37.0	37.0	37.0
10-11	36.12415229778493	37.0	37.0	37.0	37.0	37.0
12-13	36.18237166360855	37.0	37.0	37.0	37.0	37.0
14-15	36.16620606701645	37.0	37.0	37.0	37.0	37.0
16-17	36.16257179066311	37.0	37.0	37.0	37.0	37.0
18-19	36.15820066236057	37.0	37.0	37.0	37.0	37.0
20-21	36.18755703750044	37.0	37.0	37.0	37.0	37.0
22-23	36.17748457770483	37.0	37.0	37.0	37.0	37.0
24-25	36.15300883899866	37.0	37.0	37.0	37.0	37.0
26-27	36.11003440792253	37.0	37.0	37.0	37.0	37.0
28-29	36.076776103423704	37.0	37.0	37.0	37.0	37.0
30-31	36.01014179159827	37.0	37.0	37.0	37.0	37.0
32-33	35.96352502233129	37.0	37.0	37.0	37.0	37.0
34-35	35.871997368616235	37.0	37.0	37.0	33.0	37.0
36-37	35.790014930523085	37.0	37.0	37.0	33.0	37.0
38-39	35.72207298865216	37.0	37.0	37.0	33.0	37.0
40-41	35.59331415690916	37.0	37.0	37.0	33.0	37.0
42-43	35.535433388261325	37.0	37.0	37.0	33.0	37.0
44-45	35.447264296056474	37.0	37.0	37.0	33.0	37.0
46-47	35.282767338593956	37.0	37.0	37.0	33.0	37.0
48-49	35.12562761405081	37.0	37.0	37.0	33.0	37.0
50-51	35.01075610361718	37.0	37.0	37.0	30.0	37.0
52-53	34.93347532916482	37.0	37.0	37.0	27.0	37.0
54-55	34.880204319210065	37.0	37.0	37.0	27.0	37.0
56-57	34.83371815171088	37.0	37.0	37.0	27.0	37.0
58-59	34.77686607353041	37.0	37.0	37.0	27.0	37.0
60-61	34.74098767183807	37.0	37.0	37.0	27.0	37.0
62-63	34.73336439828057	37.0	37.0	37.0	27.0	37.0
64-65	34.70877418148164	37.0	37.0	37.0	27.0	37.0
66-67	34.672532997100966	37.0	37.0	37.0	27.0	37.0
68-69	34.648005662634674	37.0	37.0	37.0	27.0	37.0
70-71	34.607730334759744	37.0	37.0	37.0	27.0	37.0
72-73	34.58721295827515	37.0	37.0	37.0	27.0	37.0
74-75	34.49069502713614	37.0	37.0	37.0	27.0	37.0
76-77	34.43415736061889	37.0	37.0	37.0	27.0	37.0
78-79	34.403078976985064	37.0	37.0	37.0	27.0	37.0
80-81	34.385605750347466	37.0	37.0	37.0	27.0	37.0
82-83	34.330174167937756	37.0	37.0	37.0	27.0	37.0
84-85	34.25000725565377	37.0	37.0	37.0	27.0	37.0
86-87	34.08143100840688	37.0	37.0	37.0	27.0	37.0
88-89	34.016573525570536	37.0	37.0	37.0	27.0	37.0
90-91	33.956045249481626	37.0	37.0	37.0	27.0	37.0
92-93	33.89475109882845	37.0	37.0	37.0	22.0	37.0
94-95	33.90061689180692	37.0	37.0	37.0	22.0	37.0
96-97	33.87600087712792	37.0	37.0	37.0	22.0	37.0
98-99	33.815179150153334	37.0	37.0	37.0	22.0	37.0
100-101	33.74430753652818	37.0	35.0	37.0	22.0	37.0
102-103	33.64375546189491	37.0	33.0	37.0	22.0	37.0
104-105	33.54055265508557	37.0	33.0	37.0	22.0	37.0
106-107	33.46877489092334	37.0	33.0	37.0	18.0	37.0
108-109	33.35997716887615	37.0	33.0	37.0	14.0	37.0
110-111	33.295727548588694	37.0	33.0	37.0	14.0	37.0
112-113	33.24177450717987	37.0	33.0	37.0	14.0	37.0
114-115	33.126546663527925	37.0	33.0	37.0	14.0	37.0
116-117	32.99770398867473	37.0	33.0	37.0	14.0	37.0
118-119	32.856612157895924	37.0	33.0	37.0	14.0	37.0
120-121	32.77330757844974	37.0	33.0	37.0	14.0	37.0
122-123	32.63987933041602	37.0	33.0	37.0	14.0	37.0
124-125	31.341657449299102	37.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	217.0
16	75.0
17	17.0
18	31.0
19	222.0
20	831.0
21	2257.0
22	3563.0
23	4788.0
24	3591.0
25	2437.0
26	2397.0
27	2696.0
28	3443.0
29	4499.0
30	5933.0
31	8270.0
32	11911.0
33	17785.0
34	30285.0
35	66865.0
36	137988.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	55.19059937681842	11.689170445968351	11.648528168968655	21.471702008244577
2	18.79536799063537	19.655082343608413	35.5868856476719	25.962664018084315
3	22.508473045878603	24.553935653222663	28.5016817101525	24.435909590746242
4	15.366879284880458	17.45715925727664	34.90174200746851	32.27421945037439
5	15.834722683547406	35.191569089671944	31.970963924191466	17.00274430258919
6	29.577137771242274	34.24368189718833	18.629414287603073	17.54976604396632
7	25.94772401115823	32.1692439169905	22.93403421642453	18.94899785542674
8	25.95879433607543	31.266385684756354	21.299374723882064	21.475445255286147
9	26.642760631145133	15.728644998597241	21.269707161813976	36.35888720844365
10-11	25.744671931584023	24.80401672992522	26.256276140508156	23.195035197982605
12-13	27.00264105797106	23.984611564544682	26.348181088219075	22.664566289265178
14-15	25.040712279468437	23.854332270245692	25.5268410818341	25.57811436845177
16-17	23.462849440347238	26.087944973121836	26.541181478412014	23.908024108118916
18-19	23.3128992625031	24.707113442952824	29.15934383092069	22.820643463623377
20-21	24.67006876758491	23.751662756669166	28.8045081868092	22.773760288936725
22-23	24.746938920294227	23.560720147821854	28.76737729077113	22.924963641112793
24-25	23.725504106700033	24.42720644431044	28.876857044272384	22.97043240471714
26-27	23.392066506934793	24.5178214980184	28.79220775032812	23.29790424471869
28-29	23.01041911880891	24.3854461259646	29.48133362140966	23.12280113381683
30-31	23.527021666994514	24.162294463452465	29.383624150685417	22.9270597188676
32-33	23.25033940335953	24.293379941503307	28.92619548988562	23.530085165251545
34-35	23.42951121000314	24.209414629033947	29.1090848993478	23.251989261615112
36-37	23.51762245587332	24.322681444623775	28.744459475365325	23.41523662413758
38-39	23.445435871307275	24.200346336539795	28.908943157596024	23.445274634556906
40-41	23.18487954556082	24.23342411152657	28.980656021593276	23.60104032131933
42-43	23.581073999725895	24.318249611015712	28.45720366652962	23.643472722728774
44-45	23.582970819372918	24.29579849275886	28.34848421330977	23.772746474558453
46-47	23.513913567865124	24.34366464254272	28.020464828293445	24.121956961298707
48-49	23.85703067429264	24.479127194389843	27.96418994422416	23.69965218709335
50-51	23.485906295650157	24.832878108241456	27.583254596053568	24.09796100005482
52-53	23.341599404068972	24.958320300029346	27.185967243141796	24.51411305275989
54-55	23.488002373404964	24.89817899214132	27.006510739979944	24.60730789447377
56-57	23.71228269316969	24.697922948181734	26.668880984705083	24.920913373943495
58-59	23.329544263590265	24.788577970187276	27.441088027345796	24.44078973887666
60-61	23.63295421198763	24.695504396926182	27.634205409170505	24.037335981915685
62-63	23.239052830833625	24.786925634385994	27.51102053188779	24.463001002892586
64-65	23.03105742285628	24.65761376058922	27.59679848308465	24.71453033346985
66-67	23.635920936787485	24.54729570660569	27.370267768744295	24.446515587862528
68-69	23.409802549475497	24.21389022357088	27.630174490411253	24.746132736542375
70-71	23.199711063743337	24.366581426171305	27.993602125745316	24.44010538434004
72-73	23.642950890510573	24.26097135467893	28.182732833929368	23.913344920881126
74-75	23.50688892366921	24.32994547915897	28.153826543372222	24.009339053799597
76-77	23.30479974063303	24.36694619587045	27.988728505042975	24.33952555845354
78-79	23.04197174151044	24.686110837903076	27.988255458293494	24.28366196229299
80-81	22.82144964737523	24.706146022450607	28.210143081492276	24.26226124868189
82-83	23.133120285840512	24.368838740676484	28.41829972622	24.079741247263005
84-85	23.24427665650259	24.685478276301566	27.880709226723454	24.189535840472388
86-87	22.85959858537129	24.866510167845792	27.821138973214794	24.452752273568123
88-89	23.411589041184435	24.28517870936257	27.84659985197778	24.456632397475218
90-91	23.498667958343425	24.43303463308307	27.765237749253252	24.303059659320255
92-93	23.209659324783498	24.46921332354057	27.924875505443865	24.396251846232072
94-95	22.49143428382551	24.525116695286236	28.210511040704283	24.772937980183972
96-97	22.83241374640039	24.57506054439976	27.653070108963796	24.93945560023605
98-99	22.829672721644098	24.877218214593217	27.237240529759465	25.055868534003217
100-101	23.20261332525	24.940261783987903	26.870104449166888	24.987020441595213
102-103	23.104420144274645	24.858353514799923	27.13082427451524	24.906402066410195
104-105	23.2158606645265	24.969969413141868	26.9396534349348	24.87451648739683
106-107	23.103118565469558	24.918532898842127	26.881160420452478	25.09718811523584
108-109	23.128136166041045	25.065303200340544	26.610328547656824	25.196232085961583
110-111	22.750007255700563	25.475248386815906	26.683886862667322	25.090857494816206
112-113	22.67972254379996	25.43348500336985	26.43927985217815	25.44751260065204
114-115	22.958054191759175	25.43433219661241	26.450286598785883	25.15732701284253
116-117	23.00432921372772	25.364355334123395	26.305495763497554	25.325819688651336
118-119	22.845957633431475	25.138905460443787	26.54214889891423	25.472988007210507
120-121	22.77645025330457	25.293855872764038	26.763054617689075	25.166639256242323
122-123	22.596040670357915	25.49459373176009	26.934437912564533	24.974927685317457
124-125	22.687461907817724	25.20162655633773	26.857527982638025	25.253383553206515
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	38.0
1	31.5
2	94.5
3	161.5
4	143.0
5	136.0
6	169.5
7	300.0
8	522.5
9	682.0
10	686.0
11	613.5
12	595.0
13	624.0
14	632.5
15	651.0
16	648.5
17	624.5
18	621.0
19	618.5
20	616.5
21	620.5
22	638.0
23	645.0
24	654.5
25	648.5
26	727.5
27	850.0
28	962.5
29	1197.5
30	1607.5
31	2080.0
32	2590.5
33	3114.5
34	3674.0
35	4177.0
36	4778.5
37	5617.0
38	6214.5
39	6451.5
40	6446.5
41	6348.0
42	6491.5
43	7300.5
44	8525.0
45	9673.0
46	10894.5
47	12060.0
48	13088.0
49	14071.0
50	14693.0
51	14471.5
52	13861.5
53	13449.0
54	13089.5
55	12597.5
56	11912.0
57	10577.5
58	9339.0
59	8455.5
60	7298.5
61	6066.5
62	4910.0
63	3908.5
64	2878.0
65	2151.5
66	1664.5
67	1246.5
68	1014.0
69	886.5
70	781.5
71	660.5
72	548.0
73	508.5
74	408.5
75	334.0
76	295.0
77	207.0
78	158.0
79	119.0
80	73.5
81	54.5
82	43.5
83	29.0
84	21.0
85	16.5
86	13.5
87	9.5
88	3.5
89	2.5
90	2.0
91	1.0
92	0.5
93	2.0
94	1.5
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026120353559946213
2	0.0
3	6.449470014801533E-4
4	3.2247350074007667E-4
5	0.00193484100444046
6	6.449470014801533E-4
7	0.00580452301332138
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.6123675037003833E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	1.6123675037003833E-4
36-37	4.83710251110115E-4
38-39	0.0
40-41	0.0020960777548104984
42-43	1.6123675037003833E-4
44-45	0.0
46-47	0.003224735007400767
48-49	0.0072556537666517255
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.6123675037003833E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0070944170162816865
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.07142788041392699
76-77	0.03789063633695901
78-79	4.83710251110115E-4
80-81	0.0
82-83	0.0
84-85	0.060625018139134415
86-87	0.20073975421069776
88-89	0.2225067155106529
90-91	0.1380186583167528
92-93	0.11302696200939687
94-95	1.6123675037003833E-4
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	8.061837518501917E-4
106-107	0.002741024756290652
108-109	0.0035472085081408432
110-111	6.449470014801533E-4
112-113	0.0
114-115	1.6123675037003833E-4
116-117	1.6123675037003833E-4
118-119	0.0
120-121	6.449470014801533E-4
122-123	0.0
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	310103.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.04606381753183
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.74421735161233	56.63068051188909
2	9.304941798047874	12.291095596245787
3	2.0610985683289997	4.083823427342418
4	0.7350084806695362	1.941776680829092
5	0.4305081450619757	1.4216684211365251
6	0.2730564501254568	1.0820582238464773
7	0.17948994519434494	0.8298223062437697
8	0.15145632822926916	0.8002475455839488
9	0.10959671643935025	0.651458855533074
>10	0.8912233120766108	11.861846017631972
>50	0.09127688677713003	4.066419441412476
>100	0.026003299139996338	2.8162110163548735
>500	0.0010613591485712792	0.43320931128993445
>1k	0.0010613591485712792	1.0896826446605228
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1582	0.5101530781708014	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1527	0.4924170356300971	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	701	0.22605392401879373	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	535	0.17252332289594102	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	486	0.15672212135967725	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	405	0.13060176779973107	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	333	0.10738367574644554	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.449470014801533E-4
7	0.0	0.0	0.0	0.0	6.449470014801533E-4
8	0.0	0.0	0.0	0.0	6.449470014801533E-4
9	0.0	0.0	0.0	0.0	6.449470014801533E-4
10-11	0.0	0.0	0.0	0.0	6.449470014801533E-4
12-13	0.0	0.0	0.0	6.449470014801533E-4	6.449470014801533E-4
14-15	0.0	0.0	0.0	6.449470014801533E-4	6.449470014801533E-4
16-17	0.0	0.0	0.0	8.061837518501917E-4	6.449470014801533E-4
18-19	0.0	0.0	0.0	9.6742050222023E-4	6.449470014801533E-4
20-21	0.0	0.0	0.0	0.0020960777548104984	6.449470014801533E-4
22-23	0.0	0.0	0.0	0.0035472085081408432	6.449470014801533E-4
24-25	0.0	0.0	0.0	0.00870678451998207	6.449470014801533E-4
26-27	0.0	0.0	0.0	0.013382650280713181	6.449470014801533E-4
28-29	0.0	0.0	0.0	0.030634982570307286	6.449470014801533E-4
30-31	0.0	0.0	0.0	0.05562667887766323	6.449470014801533E-4
32-33	0.0	0.0	0.0	0.09529091946869267	6.449470014801533E-4
34-35	0.0	0.0	0.0	0.13737371131527268	6.449470014801533E-4
36-37	0.0	0.0	0.0	0.20702798747512924	6.449470014801533E-4
38-39	0.0	0.0	0.0	0.2976430411830908	9.6742050222023E-4
40-41	0.0	0.0	0.0	0.38777438463994224	9.6742050222023E-4
42-43	0.0	0.0	0.0	0.46887647007607147	9.6742050222023E-4
44-45	0.0	0.0	0.0	0.5496560820114607	9.6742050222023E-4
46-47	0.0	0.0	0.0	0.6522026552468051	9.6742050222023E-4
48-49	0.0	0.0	0.0	0.7465261542132775	9.6742050222023E-4
50-51	0.0	0.0	0.0	0.8355288404175387	9.6742050222023E-4
52-53	0.0	0.0	0.0	0.9256601838743901	0.0012898940029603067
54-55	0.0	0.0	0.0	1.0225634708467832	0.0016123675037003835
56-57	0.0	0.0	0.0	1.1255937543332375	0.0016123675037003835
58-59	0.0	0.0	0.0	1.2437802923544758	0.0016123675037003835
60-61	0.0	0.0	0.0	1.3558398338616524	0.0016123675037003835
62-63	0.0	0.0	0.0	1.4585476438473668	0.0016123675037003835
64-65	0.0	0.0	0.0	1.55561216757013	0.0016123675037003835
66-67	0.0	0.0	0.0	1.6628346065662054	0.0016123675037003835
68-69	0.0	0.0	0.0	1.7873093778518752	0.0016123675037003835
70-71	0.0	0.0	0.0	1.9225870114123371	0.0016123675037003835
72-73	0.0	0.0	0.0	2.051415174957998	0.0016123675037003835
74-75	0.0	0.0	0.0	2.1731489214873765	0.0016123675037003835
76-77	0.0	0.0	0.0	2.3103613960522793	0.0016123675037003835
78-79	0.0	0.0	0.0	2.4627301251519658	0.0016123675037003835
80-81	0.0	0.0	0.0	2.616388748254612	0.0017736042540704216
82-83	0.0	0.0	0.0	2.7793991028787213	0.00193484100444046
84-85	0.0	0.0	0.0	2.923544757709535	0.00193484100444046
86-87	0.0	0.0	0.0	3.0673679390396096	0.00193484100444046
88-89	0.0	0.0	0.0	3.2329580816696386	0.0020960777548104984
90-91	0.0	0.0	0.0	3.4083836660722406	0.0022573145051805366
92-93	0.0	0.0	0.0	3.569620416442279	0.0022573145051805366
94-95	0.0	0.0	0.0	3.7413375555863695	0.0022573145051805366
96-97	0.0	0.0	0.0	3.9286946595163545	0.0022573145051805366
98-99	0.0	0.0	0.0	4.134110279487783	0.0022573145051805366
100-101	0.0	0.0	0.0	4.355488337745846	0.0022573145051805366
102-103	0.0	0.0	0.0	4.563967455974305	0.0035472085081408437
104-105	0.0	0.0	0.0	4.765997104187964	0.0035472085081408437
106-107	0.0	0.0	0.0	4.972380144661612	0.0035472085081408437
108-109	0.0	0.0	0.0	5.192145835415975	0.0035472085081408437
110-111	0.0	0.0	0.0	5.439805483984354	0.0035472085081408437
112-113	0.0	0.0	0.0	5.711166934857128	0.0035472085081408437
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCAACG	70	9.146115E-7	50.978367	1
TATCACG	35	0.0078089708	50.970146	2
TACCCCG	35	0.0078089708	50.970146	8
GCGTAAG	60	2.1951484E-5	49.5623	1
GCGCAGA	155	0.0	46.044975	1
ACTTATG	65	0.0019422946	36.593952	8
GCCCAAC	75	0.0039142417	31.719873	1
GTATCAA	2330	0.0	31.141293	1
GGTATCA	1685	0.0	29.649141	1
ATGGGGC	285	3.6379788E-12	27.124464	5
CAACGCA	2800	0.0	26.971703	5
GGCCGAA	45	0.0095453635	26.433228	40-41
ATCAACG	2810	0.0	26.24086	3
TATCAAC	2790	0.0	26.002691	2
AACGCAG	2960	0.0	25.915565	6
TCAACGC	3000	0.0	25.371809	4
CATGGGG	1125	0.0	25.371807	4
GTGTAAA	145	1.3408504E-4	24.610247	1
GTCTTAT	170	1.3990737E-5	24.489607	1
GTGTTAT	125	0.0016348789	23.789906	1
>>END_MODULE