FastQCFastQC Report
Sun 1 Jan 2017
ERR1142450_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142450_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321229
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8760.27270265137954547No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7660.23845916775882625No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7130.22196003474157064No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6880.21417742482777083No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG4640.14444524000012451No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG3950.12296523663803705No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT3450.1074000168104374No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC3450.1074000168104374No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA1251.260396E-633.3664631
GTATAGA800.005320121529.7914851
GTATAAT1004.395531E-429.7914851
GTACAAA1852.6539223E-522.5449071
ATGGGGG8550.021.593055
GAGTGCA1400.003133898421.2696537
CATGGGG13650.020.0698264
TACCGTC1500.004673552219.8516757
TAACGTC905.7924783E-419.82688144-45
CTCTAGG1856.875511E-419.3242051
TGGGGGA5250.019.2754466
CGTTCTA800.00683303218.59205456-57
TATTCTC1650.00810365318.0469785
TGGGGGG4300.017.9965696
GTACAGG1700.0095982317.5244031
GTACAGA2050.001369925817.438921
CTATACT2553.1281044E-416.3484384
TGGGTAC2553.1393085E-416.3407756
CTATTGA2200.002194910516.2524419
GCTATAC2955.169435E-516.1505153
Produced by FastQC (version 0.11.5)