##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142450_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	321229
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.552471912560822	33.0	33.0	33.0	27.0	33.0
2	30.678142384404897	33.0	33.0	33.0	27.0	33.0
3	30.805696247848108	33.0	33.0	33.0	27.0	33.0
4	30.72039261710493	33.0	33.0	33.0	27.0	33.0
5	30.84304966239038	33.0	33.0	33.0	27.0	33.0
6	33.965750290291346	37.0	37.0	37.0	27.0	37.0
7	34.00840210566294	37.0	37.0	37.0	27.0	37.0
8	33.95008234001289	37.0	37.0	37.0	27.0	37.0
9	33.828636268830024	37.0	37.0	37.0	14.0	37.0
10-11	33.99091458118663	37.0	37.0	37.0	27.0	37.0
12-13	33.912509455871046	37.0	37.0	37.0	20.5	37.0
14-15	33.86631810951066	37.0	37.0	37.0	20.5	37.0
16-17	33.86585115291583	37.0	37.0	37.0	20.5	37.0
18-19	33.898284401470605	37.0	37.0	37.0	27.0	37.0
20-21	33.880410859542565	37.0	37.0	37.0	24.5	37.0
22-23	33.98172176235645	37.0	37.0	37.0	27.0	37.0
24-25	34.08177188236429	37.0	37.0	37.0	27.0	37.0
26-27	34.14658234468246	37.0	37.0	37.0	27.0	37.0
28-29	34.210516485124316	37.0	37.0	37.0	27.0	37.0
30-31	34.16010696419066	37.0	37.0	37.0	27.0	37.0
32-33	34.17781862783248	37.0	37.0	37.0	27.0	37.0
34-35	34.253149622232115	37.0	37.0	37.0	27.0	37.0
36-37	34.22768025302821	37.0	37.0	37.0	27.0	37.0
38-39	34.19463529133422	37.0	37.0	37.0	27.0	37.0
40-41	34.14520170968375	37.0	37.0	37.0	27.0	37.0
42-43	34.14342260505745	37.0	37.0	37.0	27.0	37.0
44-45	34.12339639322726	37.0	37.0	37.0	27.0	37.0
46-47	34.11032160857208	37.0	37.0	37.0	27.0	37.0
48-49	34.09541012797724	37.0	37.0	37.0	27.0	37.0
50-51	34.014354245725016	37.0	37.0	37.0	27.0	37.0
52-53	34.02578378664442	37.0	37.0	37.0	27.0	37.0
54-55	33.99207107701982	37.0	37.0	37.0	27.0	37.0
56-57	33.95133845325297	37.0	37.0	37.0	27.0	37.0
58-59	33.901346080210686	37.0	37.0	37.0	27.0	37.0
60-61	33.879757120309804	37.0	37.0	37.0	27.0	37.0
62-63	33.874656397772306	37.0	37.0	37.0	27.0	37.0
64-65	33.8212894850714	37.0	37.0	37.0	24.5	37.0
66-67	33.758353075220484	37.0	37.0	37.0	22.0	37.0
68-69	33.71308630291786	37.0	37.0	37.0	22.0	37.0
70-71	33.64642202291823	37.0	37.0	37.0	22.0	37.0
72-73	33.56258307936083	37.0	33.0	37.0	18.0	37.0
74-75	33.49123989428103	37.0	33.0	37.0	14.0	37.0
76-77	33.4280155278633	37.0	33.0	37.0	14.0	37.0
78-79	33.350838498392115	37.0	33.0	37.0	14.0	37.0
80-81	33.3155739363507	37.0	33.0	37.0	14.0	37.0
82-83	33.229680383776056	37.0	33.0	37.0	14.0	37.0
84-85	33.14981835388461	37.0	33.0	37.0	14.0	37.0
86-87	33.05928636580134	37.0	33.0	37.0	14.0	37.0
88-89	32.98729878062068	37.0	33.0	37.0	14.0	37.0
90-91	32.861453978314536	37.0	33.0	37.0	14.0	37.0
92-93	32.77970700030197	37.0	33.0	37.0	14.0	37.0
94-95	32.68784574244542	37.0	33.0	37.0	14.0	37.0
96-97	32.58832017034577	37.0	33.0	37.0	14.0	37.0
98-99	32.48228677983619	37.0	33.0	37.0	14.0	37.0
100-101	32.352656516067974	37.0	33.0	37.0	14.0	37.0
102-103	32.23431881928468	37.0	33.0	37.0	14.0	37.0
104-105	32.089156022650506	37.0	33.0	37.0	14.0	37.0
106-107	31.99382372077241	37.0	33.0	37.0	14.0	37.0
108-109	31.884496729747312	37.0	30.0	37.0	14.0	37.0
110-111	31.77975058291748	37.0	27.0	37.0	14.0	37.0
112-113	31.62974077682899	37.0	27.0	37.0	14.0	37.0
114-115	31.51278060200044	37.0	27.0	37.0	14.0	37.0
116-117	31.29445037652267	37.0	27.0	37.0	14.0	37.0
118-119	31.208614415261387	37.0	27.0	37.0	14.0	37.0
120-121	31.106187486185867	37.0	27.0	37.0	14.0	37.0
122-123	31.000758026205602	37.0	27.0	37.0	14.0	37.0
124-125	29.501095791475862	35.0	20.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	634.0
1	35.0
2	26.0
3	15.0
4	8.0
5	7.0
6	15.0
7	13.0
8	15.0
9	10.0
10	13.0
11	8.0
12	16.0
13	11.0
14	35.0
15	143.0
16	232.0
17	595.0
18	1370.0
19	2699.0
20	4144.0
21	4691.0
22	4746.0
23	4775.0
24	4838.0
25	5184.0
26	5805.0
27	6599.0
28	7658.0
29	8904.0
30	10471.0
31	12198.0
32	15181.0
33	19747.0
34	27787.0
35	48831.0
36	123770.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.64969982217472	20.69336233541786	11.916794019570156	23.74014382283727
2	16.9358995585851	24.13505484103753	35.37879521534987	23.550250385027507
3	19.558508411892223	29.161122252000677	27.752684746143895	23.527684589963204
4	14.23810550053253	20.481683111106946	33.67971290162383	31.600498486736694
5	14.598257381448517	38.8855738800911	30.725757826631632	15.79041091182875
6	28.610589021336647	37.070316646580956	18.007518092752285	16.311576239330115
7	25.105680193315145	34.972182850149544	21.507870598739956	18.41426635779536
8	24.88792877389708	34.95685726059213	20.240250739857398	19.914963225653384
9	25.79840459576328	19.760221046223016	19.883544857084875	34.55782950092883
10-11	24.44010483741303	28.880084794048184	24.665046338573642	22.014764029965143
12-13	25.11129675752063	27.78804038069659	24.678133368727604	22.422529493055176
14-15	24.214349880873336	27.570050384720542	23.02198961059251	25.193610123813613
16-17	23.49822273031104	29.291684626773367	23.943945726119306	23.266146916796295
18-19	23.66257971430891	28.217928458554802	25.566198010031044	22.55329381710524
20-21	25.44439673679691	26.954525937780556	24.851399352043405	22.749677973379132
22-23	25.40335045510609	26.76086243774492	24.356840642612916	23.478946464536072
24-25	24.29435326512836	27.474875823033386	24.555195139693232	23.67557577214502
26-27	24.075536850518585	27.24715417709979	24.720100907291425	23.9572080650902
28-29	23.90368471823753	27.03880145977654	25.404825754004552	23.65268806798138
30-31	24.512	27.06122926829268	25.091746341463416	23.335024390243902
32-33	23.571970441965664	27.42500444869021	25.012253409882025	23.990771699462098
34-35	23.684797859904265	27.44588501355508	25.14440807904869	23.724909047491956
36-37	23.935638039672916	27.61168055015772	24.66298001058236	23.789701399587006
38-39	23.982176579838622	27.228785916064492	24.74349570021694	24.04554180387995
40-41	23.54635146039917	27.53882197224516	25.014632867214733	23.900193700140942
42-43	23.94830239447127	27.47227363959009	24.86242169039496	23.71700227554368
44-45	23.863478522791297	27.53121737216913	24.74869254575505	23.856611559284524
46-47	23.528199941942674	27.523386802258614	25.134296585542476	23.814116670256233
48-49	23.854430824210553	27.37021980508587	25.131438291497282	23.643911079206298
50-51	23.46573777553046	27.762015344182885	24.91947113133697	23.852775748949693
52-53	23.190298944214245	27.673759861413487	24.816037330961134	24.31990386341113
54-55	23.35045424253633	27.46165743510988	24.793167867627435	24.394720454726354
56-57	23.550517117873536	26.94662457101924	24.723805837727834	24.779052473379394
58-59	23.563963677164335	26.98214288500942	25.125583442180123	24.328309995646126
60-61	23.58613048702464	27.235460262472106	25.19669803223944	23.981711218263815
62-63	23.011597712916032	27.26461261891089	25.153554218371575	24.5702354498015
64-65	22.8444630312185	26.899934148724014	25.367097456767546	24.88850536328994
66-67	23.526492856384937	26.77810165217554	24.971912391628646	24.723493099810874
68-69	23.58201129209959	26.361809107961587	24.849953651830052	25.206225948108774
70-71	23.25904379660001	26.453202585413198	25.051105916426614	25.236647701560173
72-73	23.685528604975932	26.547040420668296	25.200308947783146	24.56712202657263
74-75	23.850247350926203	26.100282463833707	25.330139359228454	24.719330826011642
76-77	23.46850221639508	26.253199725291875	25.493538115752013	24.78475994256103
78-79	23.50922478128014	26.346106427539194	25.565331926701145	24.57933686447952
80-81	23.348877714414137	26.30511934526318	25.569882605587864	24.776120334734824
82-83	23.527245260404637	26.268313846422753	25.546528250298078	24.657912642874532
84-85	23.437951112477506	26.554479951683657	25.15867454668317	24.848894389155664
86-87	23.22848836937135	26.452950841927375	25.366426829325384	24.952133959375892
88-89	23.760619748636692	25.716915923576817	25.529368627175636	24.993095700610855
90-91	23.860223391690603	26.002087568668998	25.36208706939996	24.775601970240444
92-93	23.388151222480534	26.47079933219953	25.574729681234494	24.56631976408544
94-95	22.546991425468978	26.64891227019134	25.912370040314027	24.891726264025664
96-97	23.083211259783436	26.57240872564574	25.395510501445383	24.94886951312544
98-99	23.037322234217143	26.857687531590674	25.121838592725254	24.98315164146693
100-101	23.109764753046075	27.298068388680818	24.87244002614375	24.719726832129357
102-103	23.269911442303343	27.245258177586496	25.169760284283154	24.31507009582701
104-105	23.139455432106853	27.723229467943376	24.90694547145028	24.23036962849949
106-107	22.976126592704446	27.669222897505335	25.143358999638092	24.211291510152126
108-109	23.0448367466957	27.895872298805568	24.86853784129074	24.19075311320799
110-111	22.734462748034446	28.240047422937725	24.7415137900911	24.28397603893673
112-113	22.774439515047142	28.315331673561584	24.49286485339723	24.41736395799405
114-115	22.648952082966144	28.409727215583757	24.60300501147195	24.33831568997815
116-117	22.862531237228225	28.276048182547086	24.638490504818567	24.222930075406126
118-119	22.662359741584496	27.941722374902128	24.98120529433601	24.41471258917737
120-121	22.621509743551353	28.083482286295393	25.11970827055473	24.17529969959853
122-123	22.456373473325005	28.455927421430143	25.122079412833504	23.965619692411348
124-125	22.505072749703277	28.25065388172578	24.843762428431727	24.400510940139213
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1183.0
1	700.5
2	162.5
3	104.0
4	89.5
5	73.5
6	71.5
7	70.0
8	62.5
9	64.5
10	66.5
11	67.5
12	65.5
13	73.5
14	86.0
15	99.5
16	117.0
17	127.0
18	150.0
19	174.5
20	218.5
21	274.5
22	325.0
23	415.5
24	549.5
25	688.0
26	872.0
27	1084.5
28	1296.5
29	1714.0
30	2359.0
31	2871.5
32	3514.5
33	4257.0
34	4927.0
35	5545.5
36	6193.5
37	7015.0
38	7466.5
39	7647.0
40	7626.5
41	7589.0
42	7837.5
43	8608.0
44	9593.5
45	10436.0
46	11404.5
47	12434.5
48	13685.0
49	14735.0
50	15937.5
51	15325.0
52	13404.5
53	12841.0
54	12282.5
55	11632.0
56	11026.0
57	9891.0
58	8949.0
59	8088.5
60	6879.5
61	5804.0
62	4774.5
63	3687.0
64	2806.5
65	2183.5
66	1665.5
67	1268.0
68	1035.5
69	898.0
70	791.0
71	714.5
72	644.0
73	615.0
74	493.5
75	406.0
76	312.5
77	210.0
78	151.0
79	95.0
80	72.0
81	44.5
82	33.5
83	27.5
84	15.5
85	8.5
86	4.0
87	3.0
88	3.5
89	1.5
90	0.5
91	0.5
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.38975310448309464
2	0.34928353293133557
3	0.33838787905201584
4	0.3299826603451121
5	0.3536417944830635
6	0.291069610776113
7	0.28733395801748907
8	0.2792400437071373
9	0.290758306379561
10-11	0.2874896102157651
12-13	0.3195539630606203
14-15	0.370919188491699
16-17	0.3346522262933919
18-19	0.31846439767268836
20-21	0.30865830918130055
22-23	0.3032104822416407
24-25	0.2864000448278331
26-27	0.291069610776113
28-29	0.2820417832761052
30-31	0.2853104794399011
32-33	0.2832870008623132
34-35	0.27067917280195747
36-37	0.27534873875023735
38-39	0.26896699862092155
40-41	0.27659395633644535
42-43	0.26958960741402554
44-45	0.2660096068536776
46-47	0.2664765634485056
48-49	0.25884960573298177
50-51	0.26367482387953767
52-53	0.2650756936640216
54-55	0.26787743323298957
56-57	0.2638304760778136
58-59	0.2566704749571178
60-61	0.25340177879332193
62-63	0.25558090956918583
64-65	0.25215656120711394
66-67	0.24997743043124998
68-69	0.2577600403450498
70-71	0.25402438758642587
72-73	0.24484090788814208
74-75	0.2596278667243618
76-77	0.2767496085347213
78-79	0.2621183018967777
80-81	0.2664765634485056
82-83	0.2624296062933297
84-85	0.26165134530194967
86-87	0.25013308262952594
88-89	0.2417278639226222
90-91	0.2367469935777903
92-93	0.2409496029312422
94-95	0.2319217754312344
96-97	0.22585133969847057
98-99	0.22507307870709056
100-101	0.2158895990088068
102-103	0.21900264297432673
104-105	0.2235165567243306
106-107	0.2194695995691547
108-109	0.21775742538811876
110-111	0.22071481715536267
112-113	0.21884699077605071
114-115	0.206861771508799
116-117	0.21729046879329075
118-119	0.20546090172431508
120-121	0.20483829293121109
122-123	0.1987678571984472
124-125	0.20032437918120716
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	321229.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.20419188017637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.26939497437365	63.01216139996574
2	8.560235647120535	12.361697944084336
3	1.8798850892918304	4.072067510997293
4	0.6930280418626574	2.0015811885197685
5	0.34456669778919885	1.2439579981345028
6	0.22301050470797087	0.9661375963937581
7	0.14985791198704645	0.7574258602312698
8	0.1118206631797417	0.6459136496318912
9	0.0803814658259127	0.5223490903893636
>10	0.6126552592697763	8.555740659167355
>50	0.05483644259517695	2.5876181703999683
>100	0.018436390182860453	2.2346835723002285
>500	0.0018909118136267128	1.0386653597845725
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	876	0.27270265137954547	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	766	0.23845916775882625	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	713	0.22196003474157064	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	688	0.21417742482777083	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	464	0.14444524000012451	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	395	0.12296523663803705	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	345	0.1074000168104374	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	345	0.1074000168104374	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.113043965519925E-4
2	0.0	0.0	0.0	0.0	3.113043965519925E-4
3	0.0	0.0	0.0	0.0	3.113043965519925E-4
4	0.0	0.0	0.0	0.0	3.113043965519925E-4
5	0.0	0.0	0.0	0.0	3.113043965519925E-4
6	0.0	0.0	0.0	0.0	3.113043965519925E-4
7	0.0	0.0	0.0	0.0	3.113043965519925E-4
8	0.0	0.0	0.0	0.0	3.113043965519925E-4
9	0.0	0.0	0.0	0.0	6.22608793103985E-4
10-11	0.0	0.0	0.0	1.5565219827599625E-4	6.22608793103985E-4
12-13	0.0	0.0	0.0	6.22608793103985E-4	6.22608793103985E-4
14-15	0.0	0.0	0.0	6.22608793103985E-4	9.339131896559775E-4
16-17	0.0	0.0	0.0	9.339131896559775E-4	9.339131896559775E-4
18-19	0.0	0.0	0.0	0.0014008697844839664	9.339131896559775E-4
20-21	0.0	0.0	0.0	0.0026460873706919364	9.339131896559775E-4
22-23	0.0	0.0	0.0	0.00498087034483188	9.339131896559775E-4
24-25	0.0	0.0	0.0	0.010273045086215751	9.339131896559775E-4
26-27	0.0	0.0	0.0	0.021013046767259493	9.339131896559775E-4
28-29	0.0	0.0	0.0	0.03844609297417108	9.339131896559775E-4
30-31	0.0	0.0	0.0	0.0782930557328261	9.339131896559775E-4
32-33	0.0	0.0	0.0	0.13479480370701274	9.339131896559775E-4
34-35	0.0	0.0	0.0	0.1950322044398233	9.339131896559775E-4
36-37	0.0	0.0	0.0	0.2745704777588574	9.339131896559775E-4
38-39	0.0	0.0	0.0	0.37885745060377485	9.339131896559775E-4
40-41	0.0	0.0	0.0	0.496997469095256	0.0010895653879319737
42-43	0.0	0.0	0.0	0.6061096600867294	0.00124521758620797
44-45	0.0	0.0	0.0	0.7114861983195788	0.00124521758620797
46-47	0.0	0.0	0.0	0.8355410003455479	0.00124521758620797
48-49	0.0	0.0	0.0	0.9446531913370213	0.00124521758620797
50-51	0.0	0.0	0.0	1.0487845119836627	0.00124521758620797
52-53	0.0	0.0	0.0	1.1592975727596202	0.0014008697844839664
54-55	0.0	0.0	0.0	1.2670088939666095	0.0015565219827599625
56-57	0.0	0.0	0.0	1.3781445635356708	0.0015565219827599625
58-59	0.0	0.0	0.0	1.5060906705185397	0.0015565219827599625
60-61	0.0	0.0	0.0	1.6324802555186486	0.0015565219827599625
62-63	0.0	0.0	0.0	1.7545115789670298	0.0015565219827599625
64-65	0.0	0.0	0.0	1.8633124655619513	0.0015565219827599625
66-67	0.0	0.0	0.0	1.9792733532775686	0.0015565219827599625
68-69	0.0	0.0	0.0	2.110021199829405	0.0015565219827599625
70-71	0.0	0.0	0.0	2.2600699189674653	0.0015565219827599625
72-73	0.0	0.0	0.0	2.406227333148626	0.0021791307758639474
74-75	0.0	0.0	0.0	2.533083874743563	0.0021791307758639474
76-77	0.0	0.0	0.0	2.6778404191402396	0.0021791307758639474
78-79	0.0	0.0	0.0	2.8390960965541714	0.0021791307758639474
80-81	0.0	0.0	0.0	3.0098465580629394	0.0021791307758639474
82-83	0.0	0.0	0.0	3.187134411899299	0.0021791307758639474
84-85	0.0	0.0	0.0	3.354460525045995	0.0021791307758639474
86-87	0.0	0.0	0.0	3.514782289270271	0.0021791307758639474
88-89	0.0	0.0	0.0	3.6844431853911073	0.0021791307758639474
90-91	0.0	0.0	0.0	3.8762066936671347	0.0021791307758639474
92-93	0.0	0.0	0.0	4.060498896425914	0.0021791307758639474
94-95	0.0	0.0	0.0	4.248215447546766	0.00249043517241594
96-97	0.0	0.0	0.0	4.4262815623745055	0.00249043517241594
98-99	0.0	0.0	0.0	4.631742464098821	0.00249043517241594
100-101	0.0	0.0	0.0	4.856192934012807	0.00249043517241594
102-103	0.0	0.0	0.0	5.06834688026299	0.00249043517241594
104-105	0.0	0.0	0.0	5.2812790875045525	0.00249043517241594
106-107	0.0	0.0	0.0	5.5265869519875235	0.00249043517241594
108-109	0.0	0.0	0.0	5.767069598323937	0.00249043517241594
110-111	0.0	0.0	0.0	6.006929635867247	0.0028017395689679324
112-113	0.0	0.0	0.0	6.260642719057121	0.0028017395689679324
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATA	125	1.260396E-6	33.366463	1
GTATAGA	80	0.0053201215	29.791485	1
GTATAAT	100	4.395531E-4	29.791485	1
GTACAAA	185	2.6539223E-5	22.544907	1
ATGGGGG	855	0.0	21.59305	5
GAGTGCA	140	0.0031338984	21.269653	7
CATGGGG	1365	0.0	20.069826	4
TACCGTC	150	0.0046735522	19.851675	7
TAACGTC	90	5.7924783E-4	19.826881	44-45
CTCTAGG	185	6.875511E-4	19.324205	1
TGGGGGA	525	0.0	19.275446	6
CGTTCTA	80	0.006833032	18.592054	56-57
TATTCTC	165	0.008103653	18.046978	5
TGGGGGG	430	0.0	17.996569	6
GTACAGG	170	0.00959823	17.524403	1
GTACAGA	205	0.0013699258	17.43892	1
CTATACT	255	3.1281044E-4	16.348438	4
TGGGTAC	255	3.1393085E-4	16.340775	6
CTATTGA	220	0.0021949105	16.252441	9
GCTATAC	295	5.169435E-5	16.150515	3
>>END_MODULE