FastQCFastQC Report
Sun 1 Jan 2017
ERR1142450_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142450_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321229
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19870.6185618359488091No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19150.5961479193970657No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10820.3368313570692559No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7780.24219482051745017No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7400.23036525344847444No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG5930.18460350715533155No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5590.17401915767256382No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG4930.15347306750013232No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT4460.13884176086218863No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.11487132232768522No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC3680.11456001793113324No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG1300.064.083381
GCGCAGA1700.045.504581
GTACTAG1156.632381E-736.221041
GTACAAC700.002784631734.0034261
GTTGTAT1102.033818E-532.4578131
ACCTTAC750.00391632531.716777
CTTACTC750.00391632531.716779
CGTACTT953.2911473E-431.2994442
GTGTTAT1653.3918514E-728.8513931
GTCCTAA850.007199208728.0028211
CGCATAC850.007221137627.9853863
GGTATCA28600.026.6320531
CTTAAAC900.00954319426.4306413
ATAATGC1250.001635938123.7875798
CATGGGG12700.023.4129714
GCGTACT1300.002047759222.8869231
GTATCAA35450.022.8288171
ATGGGGG8100.022.759725
GGGTACC1852.6935375E-522.5017627
CTACAGG1602.603808E-422.3147491
Produced by FastQC (version 0.11.5)