ERR1142449_2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1142449_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	71448
Sequences flagged as poor quality	0
Sequence length	125
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	285	0.39889150151158886	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	251	0.3513044451909081	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	191	0.267327286977942	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	160	0.2239390885679095	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	145	0.202944799014668	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	131	0.18335012876497592	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	126	0.17635203224722876	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	123	0.17215317433658045	No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT	117	0.16375545851528384	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	110	0.1539581233904378	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	108	0.15115888478333894	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	108	0.15115888478333894	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	103	0.14416078826559175	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	96	0.13436345314074571	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	94	0.13156421453364683	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	93	0.1301645952300974	No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	91	0.12736535662299855	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	87	0.12176687940880081	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	85	0.11896764080170194	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	84	0.11756802149815251	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	84	0.11756802149815251	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	83	0.11616840219460307	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	82	0.11476878289105362	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	78	0.10917030567685589	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	77	0.10777068637330646	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	74	0.10357182846265815	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	73	0.10217220915910873	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	73	0.10217220915910873	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAA	30	0.0041919257	59.601128	1
GGGCAAC	30	0.0041919257	59.601128	2
GGCAACT	30	0.0042036483	59.559155	3
TCTCACA	30	0.0042036483	59.559155	4
GCGCCCC	15	0.004224077	59.517242	20-21
ACTCGAG	35	0.007735888	51.050705	7
GATGCTT	65	3.4663055E-5	45.814735	5
GTTGATG	55	8.403727E-4	43.34628	2
CCTATTT	55	8.432966E-4	43.31575	3
ATGCTTC	55	8.4916863E-4	43.254826	6
CTATTTA	65	0.0019173127	36.651787	4
CTATTGA	85	1.6775608E-4	35.059486	9
TACAAAA	70	0.002748493	34.05779	2
CATGGGT	35	0.002776862	34.033806	4
CCTGACG	35	0.0027864615	33.985935	118-119
ATCCTAT	75	0.0038538428	31.787266	1
AGGGCTA	95	3.236687E-4	31.346922	5
TGGGGGA	135	2.2976128E-6	30.83909	6
ACCAGTA	80	0.0052847136	29.800564	2
CCAGTAC	80	0.0053029708	29.779577	3

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content